Detection of Prostate Cancer Biomarker PCA3 by Using Aptasensors
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.2174/0929867329666220607162250 http://hdl.handle.net/11449/247599 |
Resumo: | Background: Prostate cancer cells have very high PCA3 messenger RNA lev-els, which turns them into one of the new biomarkers for prostate cancer prognosis and di-agnosis. Objective: Our goal here is to develop a new aptasensor to detect PCA3 release by the cancer cell. Methods: DNA hairpin containing PCA3 aptamer was thiolated, conjugated to methy-lene blue (MB) redox probe, and immobilized on gold electrode through self-assembly to detect label-free cancer cells. Results: Our data have evidenced stable and sensitive sensors presenting a wide linear detection range (0-150ng/mL). In addition, monitoring PCA3 released by different types of prostate cells can provide in-depth knowledge about prostate cancer dynamics; therefore, it is a powerful platform for earlier clinical diagnostic. The released PCA3 can vary de-pending on the type of adopted prostate cells. Conclusion: PCA3 release was monitored in a group of cells for 2 h; it showed signifi-cantly higher expression in both LNCaP and PC-3 cells. This strategy provides a unique and simple methodology to achieve more sensitive and specific PCA3 detection; thus, it emerged as a promising tool for early cost-effective diagnosis. |
id |
UNSP_f1f58a318eef0499bba26fd1ac10c027 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/247599 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Detection of Prostate Cancer Biomarker PCA3 by Using AptasensorsaptamerAptasensorbiomarkercancer cellPCA3releasing proteinBackground: Prostate cancer cells have very high PCA3 messenger RNA lev-els, which turns them into one of the new biomarkers for prostate cancer prognosis and di-agnosis. Objective: Our goal here is to develop a new aptasensor to detect PCA3 release by the cancer cell. Methods: DNA hairpin containing PCA3 aptamer was thiolated, conjugated to methy-lene blue (MB) redox probe, and immobilized on gold electrode through self-assembly to detect label-free cancer cells. Results: Our data have evidenced stable and sensitive sensors presenting a wide linear detection range (0-150ng/mL). In addition, monitoring PCA3 released by different types of prostate cells can provide in-depth knowledge about prostate cancer dynamics; therefore, it is a powerful platform for earlier clinical diagnostic. The released PCA3 can vary de-pending on the type of adopted prostate cells. Conclusion: PCA3 release was monitored in a group of cells for 2 h; it showed signifi-cantly higher expression in both LNCaP and PC-3 cells. This strategy provides a unique and simple methodology to achieve more sensitive and specific PCA3 detection; thus, it emerged as a promising tool for early cost-effective diagnosis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Chemistry and Biochemistry Institute of Bioscience UNESP, SPDepartment of Chemistry and Biochemistry Institute of Bioscience UNESP, SPFAPESP: 2019/13411-5Universidade Estadual Paulista (UNESP)Crulhas, Bruno P. [UNESP]Basso, Caroline R. [UNESP]Castro, Gustavo R. [UNESP]Pedrosa, Valber A. [UNESP]2023-07-29T13:20:29Z2023-07-29T13:20:29Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5895-5902http://dx.doi.org/10.2174/0929867329666220607162250Current Medicinal Chemistry, v. 29, n. 37, p. 5895-5902, 2022.1875-533X0929-8673http://hdl.handle.net/11449/24759910.2174/09298673296662206071622502-s2.0-85137349286Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCurrent Medicinal Chemistryinfo:eu-repo/semantics/openAccess2023-07-29T13:20:29Zoai:repositorio.unesp.br:11449/247599Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:20:22.694782Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Detection of Prostate Cancer Biomarker PCA3 by Using Aptasensors |
title |
Detection of Prostate Cancer Biomarker PCA3 by Using Aptasensors |
spellingShingle |
Detection of Prostate Cancer Biomarker PCA3 by Using Aptasensors Crulhas, Bruno P. [UNESP] aptamer Aptasensor biomarker cancer cell PCA3 releasing protein |
title_short |
Detection of Prostate Cancer Biomarker PCA3 by Using Aptasensors |
title_full |
Detection of Prostate Cancer Biomarker PCA3 by Using Aptasensors |
title_fullStr |
Detection of Prostate Cancer Biomarker PCA3 by Using Aptasensors |
title_full_unstemmed |
Detection of Prostate Cancer Biomarker PCA3 by Using Aptasensors |
title_sort |
Detection of Prostate Cancer Biomarker PCA3 by Using Aptasensors |
author |
Crulhas, Bruno P. [UNESP] |
author_facet |
Crulhas, Bruno P. [UNESP] Basso, Caroline R. [UNESP] Castro, Gustavo R. [UNESP] Pedrosa, Valber A. [UNESP] |
author_role |
author |
author2 |
Basso, Caroline R. [UNESP] Castro, Gustavo R. [UNESP] Pedrosa, Valber A. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Crulhas, Bruno P. [UNESP] Basso, Caroline R. [UNESP] Castro, Gustavo R. [UNESP] Pedrosa, Valber A. [UNESP] |
dc.subject.por.fl_str_mv |
aptamer Aptasensor biomarker cancer cell PCA3 releasing protein |
topic |
aptamer Aptasensor biomarker cancer cell PCA3 releasing protein |
description |
Background: Prostate cancer cells have very high PCA3 messenger RNA lev-els, which turns them into one of the new biomarkers for prostate cancer prognosis and di-agnosis. Objective: Our goal here is to develop a new aptasensor to detect PCA3 release by the cancer cell. Methods: DNA hairpin containing PCA3 aptamer was thiolated, conjugated to methy-lene blue (MB) redox probe, and immobilized on gold electrode through self-assembly to detect label-free cancer cells. Results: Our data have evidenced stable and sensitive sensors presenting a wide linear detection range (0-150ng/mL). In addition, monitoring PCA3 released by different types of prostate cells can provide in-depth knowledge about prostate cancer dynamics; therefore, it is a powerful platform for earlier clinical diagnostic. The released PCA3 can vary de-pending on the type of adopted prostate cells. Conclusion: PCA3 release was monitored in a group of cells for 2 h; it showed signifi-cantly higher expression in both LNCaP and PC-3 cells. This strategy provides a unique and simple methodology to achieve more sensitive and specific PCA3 detection; thus, it emerged as a promising tool for early cost-effective diagnosis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-01 2023-07-29T13:20:29Z 2023-07-29T13:20:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2174/0929867329666220607162250 Current Medicinal Chemistry, v. 29, n. 37, p. 5895-5902, 2022. 1875-533X 0929-8673 http://hdl.handle.net/11449/247599 10.2174/0929867329666220607162250 2-s2.0-85137349286 |
url |
http://dx.doi.org/10.2174/0929867329666220607162250 http://hdl.handle.net/11449/247599 |
identifier_str_mv |
Current Medicinal Chemistry, v. 29, n. 37, p. 5895-5902, 2022. 1875-533X 0929-8673 10.2174/0929867329666220607162250 2-s2.0-85137349286 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Current Medicinal Chemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
5895-5902 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128498503516160 |