In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/jemt.23695 http://hdl.handle.net/11449/207099 |
Resumo: | Paraffin has been the most traditional embedding medium used in histological techniques, however it can cause several technical artifacts. On the other hand, the use of plastic resins gives a more consistent and precise support to the tissues, allowing a better perfection of the histological sections. Thus, in an attempt to retrieve samples previously embedded in paraffin, we have described this new protocol which allows the material to be deparaffinized and re-embedded into historesin (glycol methacrylate). Paraffin embedded biological materials (en bloc) were deparaffinized and re-embedded with historesin. The histological sections of the samples (in paraffin and historesin) were analyzed under light microscope and the quality of the material was compared. As expected, samples embedded in historesin showed a better quality in their morphology, even if they were previously embedded in paraffin. Therefore, this new technique proposed here allows the recovery of old materials, through reprocessing to historesin, ensuring that materials already collected that cannot be obtained again are analyzed with greater clarity and great detail. |
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Repositório Institucional da UNESP |
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In totum deparaffinization of biological samples and re-embedded in historesin for better diagnosticcytochemistryembedding mediumglycol methacrylatehistologyparaffinParaffin has been the most traditional embedding medium used in histological techniques, however it can cause several technical artifacts. On the other hand, the use of plastic resins gives a more consistent and precise support to the tissues, allowing a better perfection of the histological sections. Thus, in an attempt to retrieve samples previously embedded in paraffin, we have described this new protocol which allows the material to be deparaffinized and re-embedded into historesin (glycol methacrylate). Paraffin embedded biological materials (en bloc) were deparaffinized and re-embedded with historesin. The histological sections of the samples (in paraffin and historesin) were analyzed under light microscope and the quality of the material was compared. As expected, samples embedded in historesin showed a better quality in their morphology, even if they were previously embedded in paraffin. Therefore, this new technique proposed here allows the recovery of old materials, through reprocessing to historesin, ensuring that materials already collected that cannot be obtained again are analyzed with greater clarity and great detail.Department of Cell and Developmental Biology Institute of Biomedical Sciences Federal University of Alfenas (UNIFAL)Department of Morphology Institute of Biosciences of Botucatu São Paulo State University (UNESP)Department of Morphology Institute of Biosciences of Botucatu São Paulo State University (UNESP)Federal University of Alfenas (UNIFAL)Universidade Estadual Paulista (Unesp)Mazzoni, Talita Sarah [UNESP]Quagio-Grassiotto, Irani [UNESP]2021-06-25T10:48:59Z2021-06-25T10:48:59Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/jemt.23695Microscopy Research and Technique.1097-00291059-910Xhttp://hdl.handle.net/11449/20709910.1002/jemt.236952-s2.0-85099101836Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicroscopy Research and Techniqueinfo:eu-repo/semantics/openAccess2021-10-23T16:16:02Zoai:repositorio.unesp.br:11449/207099Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:23:36.602908Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic |
title |
In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic |
spellingShingle |
In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic Mazzoni, Talita Sarah [UNESP] cytochemistry embedding medium glycol methacrylate histology paraffin |
title_short |
In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic |
title_full |
In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic |
title_fullStr |
In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic |
title_full_unstemmed |
In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic |
title_sort |
In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic |
author |
Mazzoni, Talita Sarah [UNESP] |
author_facet |
Mazzoni, Talita Sarah [UNESP] Quagio-Grassiotto, Irani [UNESP] |
author_role |
author |
author2 |
Quagio-Grassiotto, Irani [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Federal University of Alfenas (UNIFAL) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Mazzoni, Talita Sarah [UNESP] Quagio-Grassiotto, Irani [UNESP] |
dc.subject.por.fl_str_mv |
cytochemistry embedding medium glycol methacrylate histology paraffin |
topic |
cytochemistry embedding medium glycol methacrylate histology paraffin |
description |
Paraffin has been the most traditional embedding medium used in histological techniques, however it can cause several technical artifacts. On the other hand, the use of plastic resins gives a more consistent and precise support to the tissues, allowing a better perfection of the histological sections. Thus, in an attempt to retrieve samples previously embedded in paraffin, we have described this new protocol which allows the material to be deparaffinized and re-embedded into historesin (glycol methacrylate). Paraffin embedded biological materials (en bloc) were deparaffinized and re-embedded with historesin. The histological sections of the samples (in paraffin and historesin) were analyzed under light microscope and the quality of the material was compared. As expected, samples embedded in historesin showed a better quality in their morphology, even if they were previously embedded in paraffin. Therefore, this new technique proposed here allows the recovery of old materials, through reprocessing to historesin, ensuring that materials already collected that cannot be obtained again are analyzed with greater clarity and great detail. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:48:59Z 2021-06-25T10:48:59Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/jemt.23695 Microscopy Research and Technique. 1097-0029 1059-910X http://hdl.handle.net/11449/207099 10.1002/jemt.23695 2-s2.0-85099101836 |
url |
http://dx.doi.org/10.1002/jemt.23695 http://hdl.handle.net/11449/207099 |
identifier_str_mv |
Microscopy Research and Technique. 1097-0029 1059-910X 10.1002/jemt.23695 2-s2.0-85099101836 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microscopy Research and Technique |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129062199099392 |