In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic

Detalhes bibliográficos
Autor(a) principal: Mazzoni, Talita Sarah [UNESP]
Data de Publicação: 2021
Outros Autores: Quagio-Grassiotto, Irani [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/jemt.23695
http://hdl.handle.net/11449/207099
Resumo: Paraffin has been the most traditional embedding medium used in histological techniques, however it can cause several technical artifacts. On the other hand, the use of plastic resins gives a more consistent and precise support to the tissues, allowing a better perfection of the histological sections. Thus, in an attempt to retrieve samples previously embedded in paraffin, we have described this new protocol which allows the material to be deparaffinized and re-embedded into historesin (glycol methacrylate). Paraffin embedded biological materials (en bloc) were deparaffinized and re-embedded with historesin. The histological sections of the samples (in paraffin and historesin) were analyzed under light microscope and the quality of the material was compared. As expected, samples embedded in historesin showed a better quality in their morphology, even if they were previously embedded in paraffin. Therefore, this new technique proposed here allows the recovery of old materials, through reprocessing to historesin, ensuring that materials already collected that cannot be obtained again are analyzed with greater clarity and great detail.
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spelling In totum deparaffinization of biological samples and re-embedded in historesin for better diagnosticcytochemistryembedding mediumglycol methacrylatehistologyparaffinParaffin has been the most traditional embedding medium used in histological techniques, however it can cause several technical artifacts. On the other hand, the use of plastic resins gives a more consistent and precise support to the tissues, allowing a better perfection of the histological sections. Thus, in an attempt to retrieve samples previously embedded in paraffin, we have described this new protocol which allows the material to be deparaffinized and re-embedded into historesin (glycol methacrylate). Paraffin embedded biological materials (en bloc) were deparaffinized and re-embedded with historesin. The histological sections of the samples (in paraffin and historesin) were analyzed under light microscope and the quality of the material was compared. As expected, samples embedded in historesin showed a better quality in their morphology, even if they were previously embedded in paraffin. Therefore, this new technique proposed here allows the recovery of old materials, through reprocessing to historesin, ensuring that materials already collected that cannot be obtained again are analyzed with greater clarity and great detail.Department of Cell and Developmental Biology Institute of Biomedical Sciences Federal University of Alfenas (UNIFAL)Department of Morphology Institute of Biosciences of Botucatu São Paulo State University (UNESP)Department of Morphology Institute of Biosciences of Botucatu São Paulo State University (UNESP)Federal University of Alfenas (UNIFAL)Universidade Estadual Paulista (Unesp)Mazzoni, Talita Sarah [UNESP]Quagio-Grassiotto, Irani [UNESP]2021-06-25T10:48:59Z2021-06-25T10:48:59Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/jemt.23695Microscopy Research and Technique.1097-00291059-910Xhttp://hdl.handle.net/11449/20709910.1002/jemt.236952-s2.0-85099101836Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicroscopy Research and Techniqueinfo:eu-repo/semantics/openAccess2021-10-23T16:16:02Zoai:repositorio.unesp.br:11449/207099Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:23:36.602908Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic
title In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic
spellingShingle In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic
Mazzoni, Talita Sarah [UNESP]
cytochemistry
embedding medium
glycol methacrylate
histology
paraffin
title_short In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic
title_full In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic
title_fullStr In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic
title_full_unstemmed In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic
title_sort In totum deparaffinization of biological samples and re-embedded in historesin for better diagnostic
author Mazzoni, Talita Sarah [UNESP]
author_facet Mazzoni, Talita Sarah [UNESP]
Quagio-Grassiotto, Irani [UNESP]
author_role author
author2 Quagio-Grassiotto, Irani [UNESP]
author2_role author
dc.contributor.none.fl_str_mv Federal University of Alfenas (UNIFAL)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Mazzoni, Talita Sarah [UNESP]
Quagio-Grassiotto, Irani [UNESP]
dc.subject.por.fl_str_mv cytochemistry
embedding medium
glycol methacrylate
histology
paraffin
topic cytochemistry
embedding medium
glycol methacrylate
histology
paraffin
description Paraffin has been the most traditional embedding medium used in histological techniques, however it can cause several technical artifacts. On the other hand, the use of plastic resins gives a more consistent and precise support to the tissues, allowing a better perfection of the histological sections. Thus, in an attempt to retrieve samples previously embedded in paraffin, we have described this new protocol which allows the material to be deparaffinized and re-embedded into historesin (glycol methacrylate). Paraffin embedded biological materials (en bloc) were deparaffinized and re-embedded with historesin. The histological sections of the samples (in paraffin and historesin) were analyzed under light microscope and the quality of the material was compared. As expected, samples embedded in historesin showed a better quality in their morphology, even if they were previously embedded in paraffin. Therefore, this new technique proposed here allows the recovery of old materials, through reprocessing to historesin, ensuring that materials already collected that cannot be obtained again are analyzed with greater clarity and great detail.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:48:59Z
2021-06-25T10:48:59Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/jemt.23695
Microscopy Research and Technique.
1097-0029
1059-910X
http://hdl.handle.net/11449/207099
10.1002/jemt.23695
2-s2.0-85099101836
url http://dx.doi.org/10.1002/jemt.23695
http://hdl.handle.net/11449/207099
identifier_str_mv Microscopy Research and Technique.
1097-0029
1059-910X
10.1002/jemt.23695
2-s2.0-85099101836
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microscopy Research and Technique
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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