Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca

Detalhes bibliográficos
Autor(a) principal: Morais, Glaucia Almeida de
Data de Publicação: 1998
Outros Autores: Takaki, Massanori [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1516-89131998000100003
http://hdl.handle.net/11449/26518
Resumo: Determination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.
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spelling Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. cariocadeterminationselective inactivationPhaseolus vulgarisDetermination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.UEMSUNESP Departamento de BotânicaUNESP Departamento de BotânicaBrazilian Archives of Biology and TechnologyUniversidade Estadual de Mato Grosso do Sul (UEMS)Universidade Estadual Paulista (Unesp)Morais, Glaucia Almeida deTakaki, Massanori [UNESP]2014-05-20T15:07:27Z2014-05-20T15:07:27Z1998-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17-25application/pdfhttp://dx.doi.org/10.1590/S1516-89131998000100003Brazilian Archives of Biology and Technology. Brazilian Archives of Biology and Technology, v. 41, n. 1, p. 17-25, 1998.1516-8913http://hdl.handle.net/11449/2651810.1590/S1516-89131998000100003S1516-89131998000100003S1516-89131998000100003.pdf1474830375607171SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Archives of Biology and Technology0.6760,281info:eu-repo/semantics/openAccess2023-10-30T06:07:43Zoai:repositorio.unesp.br:11449/26518Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:26:09.386799Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
title Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
spellingShingle Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
Morais, Glaucia Almeida de
determination
selective inactivation
Phaseolus vulgaris
title_short Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
title_full Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
title_fullStr Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
title_full_unstemmed Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
title_sort Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
author Morais, Glaucia Almeida de
author_facet Morais, Glaucia Almeida de
Takaki, Massanori [UNESP]
author_role author
author2 Takaki, Massanori [UNESP]
author2_role author
dc.contributor.none.fl_str_mv Universidade Estadual de Mato Grosso do Sul (UEMS)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Morais, Glaucia Almeida de
Takaki, Massanori [UNESP]
dc.subject.por.fl_str_mv determination
selective inactivation
Phaseolus vulgaris
topic determination
selective inactivation
Phaseolus vulgaris
description Determination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.
publishDate 1998
dc.date.none.fl_str_mv 1998-06-01
2014-05-20T15:07:27Z
2014-05-20T15:07:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1516-89131998000100003
Brazilian Archives of Biology and Technology. Brazilian Archives of Biology and Technology, v. 41, n. 1, p. 17-25, 1998.
1516-8913
http://hdl.handle.net/11449/26518
10.1590/S1516-89131998000100003
S1516-89131998000100003
S1516-89131998000100003.pdf
1474830375607171
url http://dx.doi.org/10.1590/S1516-89131998000100003
http://hdl.handle.net/11449/26518
identifier_str_mv Brazilian Archives of Biology and Technology. Brazilian Archives of Biology and Technology, v. 41, n. 1, p. 17-25, 1998.
1516-8913
10.1590/S1516-89131998000100003
S1516-89131998000100003
S1516-89131998000100003.pdf
1474830375607171
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Archives of Biology and Technology
0.676
0,281
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 17-25
application/pdf
dc.publisher.none.fl_str_mv Brazilian Archives of Biology and Technology
publisher.none.fl_str_mv Brazilian Archives of Biology and Technology
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128650967515136

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