Genetic diversity of Curtobacterium flaccumfaciens revealed by multilocus sequence analysis
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10658-018-01648-0 http://hdl.handle.net/11449/187168 |
Resumo: | Bacterial wilt caused by Curtobacterium flaccumfaciens pv. flaccumfaciens is among the diseases that affect Phaseolus vulgaris L. This disease has been frequently detected in bean fields and causes severe production losses in Brazil. The aim of this research was to examine the genetic diversity existing among twenty-four isolates of C. flaccumfaciens collected from their native and alternative host, and a collection of sixty strains belonging to four phytopathogenic pathovars preserved at the French Collection for Plant-associated Bacteria (CIRM-CFBP) by multilocus sequence analysis (MLSA) based on six housekeeping genes (atpD, dnaK, gyrB, ppK, recA and rpoB). A phylogenetic tree with the concatenated sequences of six genes showed high genetic diversity among the strains. For instance, strains belonging to C. f. pv. flaccumfaciens do not cluster together within the species. Similar results were obtained with a minimal MLSA scheme using gyrB and recA, which we propose for reliable identification at the species level of Curtobacterium isolates. No correlation was identified between phylogeny and pathogenicity in the Curtobacterium flaccumfaciens strains analyzed in this work. The specific primers CffFOR2 and CffREV4 designed by Tegli et al. (Letters in Applied Microbiology, 35(4), 331–337, 2002) to detect C. f. pv. flaccumfaciens in naturally infected bean seeds proved to be efficient for the detection of bean-pathogenic strains. |
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Genetic diversity of Curtobacterium flaccumfaciens revealed by multilocus sequence analysisCurtobacteriumgyrBMLSAPhylogenyrecABacterial wilt caused by Curtobacterium flaccumfaciens pv. flaccumfaciens is among the diseases that affect Phaseolus vulgaris L. This disease has been frequently detected in bean fields and causes severe production losses in Brazil. The aim of this research was to examine the genetic diversity existing among twenty-four isolates of C. flaccumfaciens collected from their native and alternative host, and a collection of sixty strains belonging to four phytopathogenic pathovars preserved at the French Collection for Plant-associated Bacteria (CIRM-CFBP) by multilocus sequence analysis (MLSA) based on six housekeeping genes (atpD, dnaK, gyrB, ppK, recA and rpoB). A phylogenetic tree with the concatenated sequences of six genes showed high genetic diversity among the strains. For instance, strains belonging to C. f. pv. flaccumfaciens do not cluster together within the species. Similar results were obtained with a minimal MLSA scheme using gyrB and recA, which we propose for reliable identification at the species level of Curtobacterium isolates. No correlation was identified between phylogeny and pathogenicity in the Curtobacterium flaccumfaciens strains analyzed in this work. The specific primers CffFOR2 and CffREV4 designed by Tegli et al. (Letters in Applied Microbiology, 35(4), 331–337, 2002) to detect C. f. pv. flaccumfaciens in naturally infected bean seeds proved to be efficient for the detection of bean-pathogenic strains.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Instituto Federal de Educação Ciência e Tecnologia de Minas Gerais, Campus Santa Luzia, Rua Érico Veríssimo, 317Departamento de Agronomia Universidade Estadual de Londrina (UEL), Rod PR 445, Km 380, C.P. 10.011Faculdade de Ciências Agronômicas Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Rod Alcides Soares, Km 3IRHS INRA AGROCAMPUS-Ouest Université d’Angers SFR 4207 QUASAV CIRM-CFBP, 42 Rue Georges MorelFaculdade de Ciências Agronômicas Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Rod Alcides Soares, Km 3Ciência e Tecnologia de Minas GeraisUniversidade Estadual de Londrina (UEL)Universidade Estadual Paulista (Unesp)CIRM-CFBPGonçalves, R. M.Balbi-Peña, M. I.Soman, J. M. [UNESP]Maringoni, A. C. [UNESP]Taghouti, G.Fischer-Le Saux, M.Portier, P.2019-10-06T15:27:32Z2019-10-06T15:27:32Z2019-06-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article189-202http://dx.doi.org/10.1007/s10658-018-01648-0European Journal of Plant Pathology, v. 154, n. 2, p. 189-202, 2019.1573-84690929-1873http://hdl.handle.net/11449/18716810.1007/s10658-018-01648-02-s2.0-85058424426Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEuropean Journal of Plant Pathologyinfo:eu-repo/semantics/openAccess2021-10-23T05:09:15Zoai:repositorio.unesp.br:11449/187168Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T05:09:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic diversity of Curtobacterium flaccumfaciens revealed by multilocus sequence analysis |
title |
Genetic diversity of Curtobacterium flaccumfaciens revealed by multilocus sequence analysis |
spellingShingle |
Genetic diversity of Curtobacterium flaccumfaciens revealed by multilocus sequence analysis Gonçalves, R. M. Curtobacterium gyrB MLSA Phylogeny recA |
title_short |
Genetic diversity of Curtobacterium flaccumfaciens revealed by multilocus sequence analysis |
title_full |
Genetic diversity of Curtobacterium flaccumfaciens revealed by multilocus sequence analysis |
title_fullStr |
Genetic diversity of Curtobacterium flaccumfaciens revealed by multilocus sequence analysis |
title_full_unstemmed |
Genetic diversity of Curtobacterium flaccumfaciens revealed by multilocus sequence analysis |
title_sort |
Genetic diversity of Curtobacterium flaccumfaciens revealed by multilocus sequence analysis |
author |
Gonçalves, R. M. |
author_facet |
Gonçalves, R. M. Balbi-Peña, M. I. Soman, J. M. [UNESP] Maringoni, A. C. [UNESP] Taghouti, G. Fischer-Le Saux, M. Portier, P. |
author_role |
author |
author2 |
Balbi-Peña, M. I. Soman, J. M. [UNESP] Maringoni, A. C. [UNESP] Taghouti, G. Fischer-Le Saux, M. Portier, P. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Ciência e Tecnologia de Minas Gerais Universidade Estadual de Londrina (UEL) Universidade Estadual Paulista (Unesp) CIRM-CFBP |
dc.contributor.author.fl_str_mv |
Gonçalves, R. M. Balbi-Peña, M. I. Soman, J. M. [UNESP] Maringoni, A. C. [UNESP] Taghouti, G. Fischer-Le Saux, M. Portier, P. |
dc.subject.por.fl_str_mv |
Curtobacterium gyrB MLSA Phylogeny recA |
topic |
Curtobacterium gyrB MLSA Phylogeny recA |
description |
Bacterial wilt caused by Curtobacterium flaccumfaciens pv. flaccumfaciens is among the diseases that affect Phaseolus vulgaris L. This disease has been frequently detected in bean fields and causes severe production losses in Brazil. The aim of this research was to examine the genetic diversity existing among twenty-four isolates of C. flaccumfaciens collected from their native and alternative host, and a collection of sixty strains belonging to four phytopathogenic pathovars preserved at the French Collection for Plant-associated Bacteria (CIRM-CFBP) by multilocus sequence analysis (MLSA) based on six housekeeping genes (atpD, dnaK, gyrB, ppK, recA and rpoB). A phylogenetic tree with the concatenated sequences of six genes showed high genetic diversity among the strains. For instance, strains belonging to C. f. pv. flaccumfaciens do not cluster together within the species. Similar results were obtained with a minimal MLSA scheme using gyrB and recA, which we propose for reliable identification at the species level of Curtobacterium isolates. No correlation was identified between phylogeny and pathogenicity in the Curtobacterium flaccumfaciens strains analyzed in this work. The specific primers CffFOR2 and CffREV4 designed by Tegli et al. (Letters in Applied Microbiology, 35(4), 331–337, 2002) to detect C. f. pv. flaccumfaciens in naturally infected bean seeds proved to be efficient for the detection of bean-pathogenic strains. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:27:32Z 2019-10-06T15:27:32Z 2019-06-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10658-018-01648-0 European Journal of Plant Pathology, v. 154, n. 2, p. 189-202, 2019. 1573-8469 0929-1873 http://hdl.handle.net/11449/187168 10.1007/s10658-018-01648-0 2-s2.0-85058424426 |
url |
http://dx.doi.org/10.1007/s10658-018-01648-0 http://hdl.handle.net/11449/187168 |
identifier_str_mv |
European Journal of Plant Pathology, v. 154, n. 2, p. 189-202, 2019. 1573-8469 0929-1873 10.1007/s10658-018-01648-0 2-s2.0-85058424426 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
European Journal of Plant Pathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
189-202 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046898302451712 |