Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.apsusc.2019.05.154 http://hdl.handle.net/11449/189185 |
Resumo: | Percutaneous medical devices are prone to bacterial contamination that causes dramatic clinical conditions. At the percutaneous level of dental implant systems, microbial pathogens induce biofilm formation that may result in bone resorption and dental implant loss. In view of peri-implantitis caused by bacterial inflammation at the percutaneous abutment region, we here establish a novel drug release system based on layer-by-layer (LbL)-deposited poly(acrylic acid) (PAA) and poly-L-lysine (PLL) coatings on titanium (Ti). Detailed multilayer coating characterization was performed by different microscopy and spectroscopy techniques to probe physical and chemical properties. Our data revealed a significant difference in roughness average between ten double layers coated (141 nm ±30) and uncoated Ti discs (115 nm ±40). Although roughness of the coatings increased significantly after immersion in water for 24 h at 37 °C, this physical property remained below 200 nm. Coating stability was confirmed under neutral and acidic pH, mimicking healthy and diseased/inflammatory environments, respectively. LbL coatings supported in vitro human keratinocytes growth, demonstrating absence of cytotoxic effects. Tetracycline (TC) showed an initial burst release under neutral and acidic conditions, which further demonstrated robust antibacterial efficacy against Porphyromonas gingivalis. However, a convenient pH-dependent 2-folds increase in TC release was observed for coatings incubated at pH = 4.5. Sustained TC release was observed from coatings up till 15 days of incubation in both pH conditions. These results demonstrate the potential application of this simple surface modification to leverage anti-bacterial efficacy at the percutaneous abutment region. |
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Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant componentsAnti-bacterialLayer-by-layerPorphyromonas gingivalisSurface modificationPercutaneous medical devices are prone to bacterial contamination that causes dramatic clinical conditions. At the percutaneous level of dental implant systems, microbial pathogens induce biofilm formation that may result in bone resorption and dental implant loss. In view of peri-implantitis caused by bacterial inflammation at the percutaneous abutment region, we here establish a novel drug release system based on layer-by-layer (LbL)-deposited poly(acrylic acid) (PAA) and poly-L-lysine (PLL) coatings on titanium (Ti). Detailed multilayer coating characterization was performed by different microscopy and spectroscopy techniques to probe physical and chemical properties. Our data revealed a significant difference in roughness average between ten double layers coated (141 nm ±30) and uncoated Ti discs (115 nm ±40). Although roughness of the coatings increased significantly after immersion in water for 24 h at 37 °C, this physical property remained below 200 nm. Coating stability was confirmed under neutral and acidic pH, mimicking healthy and diseased/inflammatory environments, respectively. LbL coatings supported in vitro human keratinocytes growth, demonstrating absence of cytotoxic effects. Tetracycline (TC) showed an initial burst release under neutral and acidic conditions, which further demonstrated robust antibacterial efficacy against Porphyromonas gingivalis. However, a convenient pH-dependent 2-folds increase in TC release was observed for coatings incubated at pH = 4.5. Sustained TC release was observed from coatings up till 15 days of incubation in both pH conditions. These results demonstrate the potential application of this simple surface modification to leverage anti-bacterial efficacy at the percutaneous abutment region.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Regenerative Biomaterials Radboudumc, Philips van Leydenlaan 25Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara Sao Paulo State University (Unesp), Humaita, Sao PauloDepartment of Tumor Immunology Radboudumc and Radboud Institute for Molecular Life Sciences (RIMLS), Geert Grooteplein ZuidDepartment of Preventive Dentistry Academic Centre for Dentistry Amsterdam (ACTA) University of Amsterdam and Vrije Universiteit Amsterdam, Gustav MahlerlaanDepartment of Organic Chemistry Radboud University, Heyendaalseweg 135Faculty of Applied Sciences Delft University of Technology, Lorentzweg 1Department of Dental Materials and Prosthodontics School of Dentistry at Araraquara Sao Paulo State University (Unesp), Humaita, Sao PauloFAPESP: 2015/03567-7FAPESP: 2016/19650-3RadboudumcUniversidade Estadual Paulista (Unesp)Radboudumc and Radboud Institute for Molecular Life Sciences (RIMLS)University of Amsterdam and Vrije Universiteit AmsterdamRadboud UniversityDelft University of Technologyde Avila, Erica D. [UNESP]Castro, Antonio G.B.Tagit, OyaKrom, Bastiaan P.Löwik, Dennisvan Well, Ad A.Bannenberg, Lars J.Vergani, Carlos Eduardo [UNESP]van den Beucken, Jeroen J.J.P.2019-10-06T16:32:33Z2019-10-06T16:32:33Z2019-09-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article194-204http://dx.doi.org/10.1016/j.apsusc.2019.05.154Applied Surface Science, v. 488, p. 194-204.0169-4332http://hdl.handle.net/11449/18918510.1016/j.apsusc.2019.05.1542-s2.0-85066443452Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied Surface Scienceinfo:eu-repo/semantics/openAccess2021-10-23T19:49:55Zoai:repositorio.unesp.br:11449/189185Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:49:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components |
title |
Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components |
spellingShingle |
Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components de Avila, Erica D. [UNESP] Anti-bacterial Layer-by-layer Porphyromonas gingivalis Surface modification |
title_short |
Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components |
title_full |
Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components |
title_fullStr |
Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components |
title_full_unstemmed |
Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components |
title_sort |
Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components |
author |
de Avila, Erica D. [UNESP] |
author_facet |
de Avila, Erica D. [UNESP] Castro, Antonio G.B. Tagit, Oya Krom, Bastiaan P. Löwik, Dennis van Well, Ad A. Bannenberg, Lars J. Vergani, Carlos Eduardo [UNESP] van den Beucken, Jeroen J.J.P. |
author_role |
author |
author2 |
Castro, Antonio G.B. Tagit, Oya Krom, Bastiaan P. Löwik, Dennis van Well, Ad A. Bannenberg, Lars J. Vergani, Carlos Eduardo [UNESP] van den Beucken, Jeroen J.J.P. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Radboudumc Universidade Estadual Paulista (Unesp) Radboudumc and Radboud Institute for Molecular Life Sciences (RIMLS) University of Amsterdam and Vrije Universiteit Amsterdam Radboud University Delft University of Technology |
dc.contributor.author.fl_str_mv |
de Avila, Erica D. [UNESP] Castro, Antonio G.B. Tagit, Oya Krom, Bastiaan P. Löwik, Dennis van Well, Ad A. Bannenberg, Lars J. Vergani, Carlos Eduardo [UNESP] van den Beucken, Jeroen J.J.P. |
dc.subject.por.fl_str_mv |
Anti-bacterial Layer-by-layer Porphyromonas gingivalis Surface modification |
topic |
Anti-bacterial Layer-by-layer Porphyromonas gingivalis Surface modification |
description |
Percutaneous medical devices are prone to bacterial contamination that causes dramatic clinical conditions. At the percutaneous level of dental implant systems, microbial pathogens induce biofilm formation that may result in bone resorption and dental implant loss. In view of peri-implantitis caused by bacterial inflammation at the percutaneous abutment region, we here establish a novel drug release system based on layer-by-layer (LbL)-deposited poly(acrylic acid) (PAA) and poly-L-lysine (PLL) coatings on titanium (Ti). Detailed multilayer coating characterization was performed by different microscopy and spectroscopy techniques to probe physical and chemical properties. Our data revealed a significant difference in roughness average between ten double layers coated (141 nm ±30) and uncoated Ti discs (115 nm ±40). Although roughness of the coatings increased significantly after immersion in water for 24 h at 37 °C, this physical property remained below 200 nm. Coating stability was confirmed under neutral and acidic pH, mimicking healthy and diseased/inflammatory environments, respectively. LbL coatings supported in vitro human keratinocytes growth, demonstrating absence of cytotoxic effects. Tetracycline (TC) showed an initial burst release under neutral and acidic conditions, which further demonstrated robust antibacterial efficacy against Porphyromonas gingivalis. However, a convenient pH-dependent 2-folds increase in TC release was observed for coatings incubated at pH = 4.5. Sustained TC release was observed from coatings up till 15 days of incubation in both pH conditions. These results demonstrate the potential application of this simple surface modification to leverage anti-bacterial efficacy at the percutaneous abutment region. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:32:33Z 2019-10-06T16:32:33Z 2019-09-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.apsusc.2019.05.154 Applied Surface Science, v. 488, p. 194-204. 0169-4332 http://hdl.handle.net/11449/189185 10.1016/j.apsusc.2019.05.154 2-s2.0-85066443452 |
url |
http://dx.doi.org/10.1016/j.apsusc.2019.05.154 http://hdl.handle.net/11449/189185 |
identifier_str_mv |
Applied Surface Science, v. 488, p. 194-204. 0169-4332 10.1016/j.apsusc.2019.05.154 2-s2.0-85066443452 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Applied Surface Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
194-204 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964639450103808 |