Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses

Detalhes bibliográficos
Autor(a) principal: Quadros, Ayane F. F.
Data de Publicação: 2022
Outros Autores: Ferro, Camila G., Rezende, Rafael R. de, Godinho, Marcio T., Xavier, Cesar A. D., Nogueira, Angelica M. [UNESP], Alfenas-Zerbini, P., Zerbini, F. Murilo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.virusres.2022.198969
http://hdl.handle.net/11449/245548
Resumo: Begomoviruses (single-stranded DNA plant viruses transmitted by whiteflies) are economically important pathogens causing epidemics worldwide. Tomato-infecting begomoviruses emerged in Brazil in the 1990 ' s following the introduction of Bemisia tabaci Middle East-Asia Minor 1. It is believed that these viruses evolved from indigenous viruses infecting non-cultivated hosts. However, tomato-infecting viruses are rarely found in non-cultivated hosts, and vice-versa. It is possible that viral populations in a given host are composed primarily of viruses which are well adapted to this host, but also include a small proportion of poorly adapted viruses. Following transfer to a new host, the composition of the viral population would shift rapidly, with the viruses which are better adapted to the new host becoming predominant. To test this hypothesis, we collected tomato and Sida plants growing next to each other at two locations in 2014 and 2018. Total DNA was extracted from tomato and Sida samples from each location and year and used as a template for high-throughput sequencing. Reads were mapped following a highly stringent set of criteria. For the 2014 samples, >98% of the Sida reads mapped to Sida micrantha mosaic virus (SiMMV), but 0.1% of the reads mapped to tomato severe rugose virus (ToSRV). Conversely, >99% of the tomato reads mapped to ToSRV, with 0.18% mapping to SiMMV. For the 2018 samples, 41% of the Sida reads mapped to three Sida-adapted viruses and 0.1% of the reads mapped to ToSRV, while 99.9% of the tomato reads mapped to ToSRV. These results are consistent with the hypothesis that viral populations in a single plant are composed primarily of the virus that is better adapted to the host but also include a small proportion of viruses that are poorly adapted.
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spelling Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted virusesGeminivirusBegomovirusNon -cultivated hostHTSQuasispeciesBegomoviruses (single-stranded DNA plant viruses transmitted by whiteflies) are economically important pathogens causing epidemics worldwide. Tomato-infecting begomoviruses emerged in Brazil in the 1990 ' s following the introduction of Bemisia tabaci Middle East-Asia Minor 1. It is believed that these viruses evolved from indigenous viruses infecting non-cultivated hosts. However, tomato-infecting viruses are rarely found in non-cultivated hosts, and vice-versa. It is possible that viral populations in a given host are composed primarily of viruses which are well adapted to this host, but also include a small proportion of poorly adapted viruses. Following transfer to a new host, the composition of the viral population would shift rapidly, with the viruses which are better adapted to the new host becoming predominant. To test this hypothesis, we collected tomato and Sida plants growing next to each other at two locations in 2014 and 2018. Total DNA was extracted from tomato and Sida samples from each location and year and used as a template for high-throughput sequencing. Reads were mapped following a highly stringent set of criteria. For the 2014 samples, >98% of the Sida reads mapped to Sida micrantha mosaic virus (SiMMV), but 0.1% of the reads mapped to tomato severe rugose virus (ToSRV). Conversely, >99% of the tomato reads mapped to ToSRV, with 0.18% mapping to SiMMV. For the 2018 samples, 41% of the Sida reads mapped to three Sida-adapted viruses and 0.1% of the reads mapped to ToSRV, while 99.9% of the tomato reads mapped to ToSRV. These results are consistent with the hypothesis that viral populations in a single plant are composed primarily of the virus that is better adapted to the host but also include a small proportion of viruses that are poorly adapted.Coordenação de Aperfeiçoamento de Pessoa de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Univ Fed Vicosa, Dep Fitopatol, BR-36570900 Vicosa, MG, BrazilUniv Fed Vicosa, Dep Microbiol, BR-36570900 Vicosa, MG, BrazilUniv Sao Paulo, Dep Fitopatol & Nematol, Piracicaba, SP, BrazilNorth Carolina State Univ, Dep Entomol & Plant Pathol, Raleigh, NC USAUniv Estadual Paulista UNESP, Fac Ciencias Agron, Dep Protecao Vegetal, Botucatu, SP, BrazilUniv Estadual Paulista UNESP, Fac Ciencias Agron, Dep Protecao Vegetal, Botucatu, SP, Brazil: 001Elsevier B.V.Universidade Federal de Viçosa (UFV)Universidade de São Paulo (USP)North Carolina State UnivUniversidade Estadual Paulista (UNESP)Quadros, Ayane F. F.Ferro, Camila G.Rezende, Rafael R. deGodinho, Marcio T.Xavier, Cesar A. D.Nogueira, Angelica M. [UNESP]Alfenas-Zerbini, P.Zerbini, F. Murilo2023-07-29T11:58:06Z2023-07-29T11:58:06Z2022-11-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9http://dx.doi.org/10.1016/j.virusres.2022.198969Virus Research. Amsterdam: Elsevier, v. 323, 9 p., 2023.0168-1702http://hdl.handle.net/11449/24554810.1016/j.virusres.2022.198969WOS:000896582500005Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVirus Researchinfo:eu-repo/semantics/openAccess2024-04-30T18:06:52Zoai:repositorio.unesp.br:11449/245548Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:53:59.091293Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses
title Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses
spellingShingle Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses
Quadros, Ayane F. F.
Geminivirus
Begomovirus
Non -cultivated host
HTS
Quasispecies
title_short Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses
title_full Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses
title_fullStr Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses
title_full_unstemmed Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses
title_sort Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses
author Quadros, Ayane F. F.
author_facet Quadros, Ayane F. F.
Ferro, Camila G.
Rezende, Rafael R. de
Godinho, Marcio T.
Xavier, Cesar A. D.
Nogueira, Angelica M. [UNESP]
Alfenas-Zerbini, P.
Zerbini, F. Murilo
author_role author
author2 Ferro, Camila G.
Rezende, Rafael R. de
Godinho, Marcio T.
Xavier, Cesar A. D.
Nogueira, Angelica M. [UNESP]
Alfenas-Zerbini, P.
Zerbini, F. Murilo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Viçosa (UFV)
Universidade de São Paulo (USP)
North Carolina State Univ
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Quadros, Ayane F. F.
Ferro, Camila G.
Rezende, Rafael R. de
Godinho, Marcio T.
Xavier, Cesar A. D.
Nogueira, Angelica M. [UNESP]
Alfenas-Zerbini, P.
Zerbini, F. Murilo
dc.subject.por.fl_str_mv Geminivirus
Begomovirus
Non -cultivated host
HTS
Quasispecies
topic Geminivirus
Begomovirus
Non -cultivated host
HTS
Quasispecies
description Begomoviruses (single-stranded DNA plant viruses transmitted by whiteflies) are economically important pathogens causing epidemics worldwide. Tomato-infecting begomoviruses emerged in Brazil in the 1990 ' s following the introduction of Bemisia tabaci Middle East-Asia Minor 1. It is believed that these viruses evolved from indigenous viruses infecting non-cultivated hosts. However, tomato-infecting viruses are rarely found in non-cultivated hosts, and vice-versa. It is possible that viral populations in a given host are composed primarily of viruses which are well adapted to this host, but also include a small proportion of poorly adapted viruses. Following transfer to a new host, the composition of the viral population would shift rapidly, with the viruses which are better adapted to the new host becoming predominant. To test this hypothesis, we collected tomato and Sida plants growing next to each other at two locations in 2014 and 2018. Total DNA was extracted from tomato and Sida samples from each location and year and used as a template for high-throughput sequencing. Reads were mapped following a highly stringent set of criteria. For the 2014 samples, >98% of the Sida reads mapped to Sida micrantha mosaic virus (SiMMV), but 0.1% of the reads mapped to tomato severe rugose virus (ToSRV). Conversely, >99% of the tomato reads mapped to ToSRV, with 0.18% mapping to SiMMV. For the 2018 samples, 41% of the Sida reads mapped to three Sida-adapted viruses and 0.1% of the reads mapped to ToSRV, while 99.9% of the tomato reads mapped to ToSRV. These results are consistent with the hypothesis that viral populations in a single plant are composed primarily of the virus that is better adapted to the host but also include a small proportion of viruses that are poorly adapted.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-07
2023-07-29T11:58:06Z
2023-07-29T11:58:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.virusres.2022.198969
Virus Research. Amsterdam: Elsevier, v. 323, 9 p., 2023.
0168-1702
http://hdl.handle.net/11449/245548
10.1016/j.virusres.2022.198969
WOS:000896582500005
url http://dx.doi.org/10.1016/j.virusres.2022.198969
http://hdl.handle.net/11449/245548
identifier_str_mv Virus Research. Amsterdam: Elsevier, v. 323, 9 p., 2023.
0168-1702
10.1016/j.virusres.2022.198969
WOS:000896582500005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Virus Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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