Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.virusres.2022.198969 http://hdl.handle.net/11449/245548 |
Resumo: | Begomoviruses (single-stranded DNA plant viruses transmitted by whiteflies) are economically important pathogens causing epidemics worldwide. Tomato-infecting begomoviruses emerged in Brazil in the 1990 ' s following the introduction of Bemisia tabaci Middle East-Asia Minor 1. It is believed that these viruses evolved from indigenous viruses infecting non-cultivated hosts. However, tomato-infecting viruses are rarely found in non-cultivated hosts, and vice-versa. It is possible that viral populations in a given host are composed primarily of viruses which are well adapted to this host, but also include a small proportion of poorly adapted viruses. Following transfer to a new host, the composition of the viral population would shift rapidly, with the viruses which are better adapted to the new host becoming predominant. To test this hypothesis, we collected tomato and Sida plants growing next to each other at two locations in 2014 and 2018. Total DNA was extracted from tomato and Sida samples from each location and year and used as a template for high-throughput sequencing. Reads were mapped following a highly stringent set of criteria. For the 2014 samples, >98% of the Sida reads mapped to Sida micrantha mosaic virus (SiMMV), but 0.1% of the reads mapped to tomato severe rugose virus (ToSRV). Conversely, >99% of the tomato reads mapped to ToSRV, with 0.18% mapping to SiMMV. For the 2018 samples, 41% of the Sida reads mapped to three Sida-adapted viruses and 0.1% of the reads mapped to ToSRV, while 99.9% of the tomato reads mapped to ToSRV. These results are consistent with the hypothesis that viral populations in a single plant are composed primarily of the virus that is better adapted to the host but also include a small proportion of viruses that are poorly adapted. |
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Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted virusesGeminivirusBegomovirusNon -cultivated hostHTSQuasispeciesBegomoviruses (single-stranded DNA plant viruses transmitted by whiteflies) are economically important pathogens causing epidemics worldwide. Tomato-infecting begomoviruses emerged in Brazil in the 1990 ' s following the introduction of Bemisia tabaci Middle East-Asia Minor 1. It is believed that these viruses evolved from indigenous viruses infecting non-cultivated hosts. However, tomato-infecting viruses are rarely found in non-cultivated hosts, and vice-versa. It is possible that viral populations in a given host are composed primarily of viruses which are well adapted to this host, but also include a small proportion of poorly adapted viruses. Following transfer to a new host, the composition of the viral population would shift rapidly, with the viruses which are better adapted to the new host becoming predominant. To test this hypothesis, we collected tomato and Sida plants growing next to each other at two locations in 2014 and 2018. Total DNA was extracted from tomato and Sida samples from each location and year and used as a template for high-throughput sequencing. Reads were mapped following a highly stringent set of criteria. For the 2014 samples, >98% of the Sida reads mapped to Sida micrantha mosaic virus (SiMMV), but 0.1% of the reads mapped to tomato severe rugose virus (ToSRV). Conversely, >99% of the tomato reads mapped to ToSRV, with 0.18% mapping to SiMMV. For the 2018 samples, 41% of the Sida reads mapped to three Sida-adapted viruses and 0.1% of the reads mapped to ToSRV, while 99.9% of the tomato reads mapped to ToSRV. These results are consistent with the hypothesis that viral populations in a single plant are composed primarily of the virus that is better adapted to the host but also include a small proportion of viruses that are poorly adapted.Coordenação de Aperfeiçoamento de Pessoa de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Univ Fed Vicosa, Dep Fitopatol, BR-36570900 Vicosa, MG, BrazilUniv Fed Vicosa, Dep Microbiol, BR-36570900 Vicosa, MG, BrazilUniv Sao Paulo, Dep Fitopatol & Nematol, Piracicaba, SP, BrazilNorth Carolina State Univ, Dep Entomol & Plant Pathol, Raleigh, NC USAUniv Estadual Paulista UNESP, Fac Ciencias Agron, Dep Protecao Vegetal, Botucatu, SP, BrazilUniv Estadual Paulista UNESP, Fac Ciencias Agron, Dep Protecao Vegetal, Botucatu, SP, Brazil: 001Elsevier B.V.Universidade Federal de Viçosa (UFV)Universidade de São Paulo (USP)North Carolina State UnivUniversidade Estadual Paulista (UNESP)Quadros, Ayane F. F.Ferro, Camila G.Rezende, Rafael R. deGodinho, Marcio T.Xavier, Cesar A. D.Nogueira, Angelica M. [UNESP]Alfenas-Zerbini, P.Zerbini, F. Murilo2023-07-29T11:58:06Z2023-07-29T11:58:06Z2022-11-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9http://dx.doi.org/10.1016/j.virusres.2022.198969Virus Research. Amsterdam: Elsevier, v. 323, 9 p., 2023.0168-1702http://hdl.handle.net/11449/24554810.1016/j.virusres.2022.198969WOS:000896582500005Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVirus Researchinfo:eu-repo/semantics/openAccess2024-04-30T18:06:52Zoai:repositorio.unesp.br:11449/245548Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:53:59.091293Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses |
title |
Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses |
spellingShingle |
Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses Quadros, Ayane F. F. Geminivirus Begomovirus Non -cultivated host HTS Quasispecies |
title_short |
Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses |
title_full |
Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses |
title_fullStr |
Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses |
title_full_unstemmed |
Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses |
title_sort |
Begomovirus populations in single plants are complex and may include both well-adapted and poorly-adapted viruses |
author |
Quadros, Ayane F. F. |
author_facet |
Quadros, Ayane F. F. Ferro, Camila G. Rezende, Rafael R. de Godinho, Marcio T. Xavier, Cesar A. D. Nogueira, Angelica M. [UNESP] Alfenas-Zerbini, P. Zerbini, F. Murilo |
author_role |
author |
author2 |
Ferro, Camila G. Rezende, Rafael R. de Godinho, Marcio T. Xavier, Cesar A. D. Nogueira, Angelica M. [UNESP] Alfenas-Zerbini, P. Zerbini, F. Murilo |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Viçosa (UFV) Universidade de São Paulo (USP) North Carolina State Univ Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Quadros, Ayane F. F. Ferro, Camila G. Rezende, Rafael R. de Godinho, Marcio T. Xavier, Cesar A. D. Nogueira, Angelica M. [UNESP] Alfenas-Zerbini, P. Zerbini, F. Murilo |
dc.subject.por.fl_str_mv |
Geminivirus Begomovirus Non -cultivated host HTS Quasispecies |
topic |
Geminivirus Begomovirus Non -cultivated host HTS Quasispecies |
description |
Begomoviruses (single-stranded DNA plant viruses transmitted by whiteflies) are economically important pathogens causing epidemics worldwide. Tomato-infecting begomoviruses emerged in Brazil in the 1990 ' s following the introduction of Bemisia tabaci Middle East-Asia Minor 1. It is believed that these viruses evolved from indigenous viruses infecting non-cultivated hosts. However, tomato-infecting viruses are rarely found in non-cultivated hosts, and vice-versa. It is possible that viral populations in a given host are composed primarily of viruses which are well adapted to this host, but also include a small proportion of poorly adapted viruses. Following transfer to a new host, the composition of the viral population would shift rapidly, with the viruses which are better adapted to the new host becoming predominant. To test this hypothesis, we collected tomato and Sida plants growing next to each other at two locations in 2014 and 2018. Total DNA was extracted from tomato and Sida samples from each location and year and used as a template for high-throughput sequencing. Reads were mapped following a highly stringent set of criteria. For the 2014 samples, >98% of the Sida reads mapped to Sida micrantha mosaic virus (SiMMV), but 0.1% of the reads mapped to tomato severe rugose virus (ToSRV). Conversely, >99% of the tomato reads mapped to ToSRV, with 0.18% mapping to SiMMV. For the 2018 samples, 41% of the Sida reads mapped to three Sida-adapted viruses and 0.1% of the reads mapped to ToSRV, while 99.9% of the tomato reads mapped to ToSRV. These results are consistent with the hypothesis that viral populations in a single plant are composed primarily of the virus that is better adapted to the host but also include a small proportion of viruses that are poorly adapted. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-07 2023-07-29T11:58:06Z 2023-07-29T11:58:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.virusres.2022.198969 Virus Research. Amsterdam: Elsevier, v. 323, 9 p., 2023. 0168-1702 http://hdl.handle.net/11449/245548 10.1016/j.virusres.2022.198969 WOS:000896582500005 |
url |
http://dx.doi.org/10.1016/j.virusres.2022.198969 http://hdl.handle.net/11449/245548 |
identifier_str_mv |
Virus Research. Amsterdam: Elsevier, v. 323, 9 p., 2023. 0168-1702 10.1016/j.virusres.2022.198969 WOS:000896582500005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Virus Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128433438326784 |