Assessment of the relationship between the MLST genetic diversity of Listeria monocytogenes and growth under selective and non-selective conditions
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.fm.2023.104303 http://hdl.handle.net/11449/247308 |
Resumo: | Listeria monocytogenes can grow under stressful conditions and contaminate various food categories. Progresss in DNA sequencing-based identification methods, such as multi-locus sequence typing (MLST) now allow for more accurate characterization of pathogens. L. monocytogenes MLST genetic diversity is reflected by the different prevalence of the “clonal complexes” (CCs) in foods or infections. Better understanding of the growth potentials of L. monocytogenes is essential for quantitative risk assessment and efficient detection across CCs genetic diversity. Using optical density measurements taken with an automated spectrophotometer, we compared the maximal growth rate and lag phase of 39 strains from 13 different CCs and various food origins, in 3 broths mimicking stresful food conditions (8 °C, aw 0.95 and pH5) and in ISO Standard enrichment broths (Half Fraser and Fraser). This is important as growth could influence risk through pathogen multiplication in food. Besides, enrichment problems could lead to a lack of detection of some CCs. Despite small differences highlighting natural intraspecific variability, our results show that growth performances of L. monocytogenes strains under the conditions tested in selective and non-selective broth do not appear to be strongly correlated to CCs and cannot explain higher CC “virulence” or prevalence. |
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Assessment of the relationship between the MLST genetic diversity of Listeria monocytogenes and growth under selective and non-selective conditionsClonal complexGrowthListeria monocytogenesMLSTSelective enrichmentListeria monocytogenes can grow under stressful conditions and contaminate various food categories. Progresss in DNA sequencing-based identification methods, such as multi-locus sequence typing (MLST) now allow for more accurate characterization of pathogens. L. monocytogenes MLST genetic diversity is reflected by the different prevalence of the “clonal complexes” (CCs) in foods or infections. Better understanding of the growth potentials of L. monocytogenes is essential for quantitative risk assessment and efficient detection across CCs genetic diversity. Using optical density measurements taken with an automated spectrophotometer, we compared the maximal growth rate and lag phase of 39 strains from 13 different CCs and various food origins, in 3 broths mimicking stresful food conditions (8 °C, aw 0.95 and pH5) and in ISO Standard enrichment broths (Half Fraser and Fraser). This is important as growth could influence risk through pathogen multiplication in food. Besides, enrichment problems could lead to a lack of detection of some CCs. Despite small differences highlighting natural intraspecific variability, our results show that growth performances of L. monocytogenes strains under the conditions tested in selective and non-selective broth do not appear to be strongly correlated to CCs and cannot explain higher CC “virulence” or prevalence.Agence Nationale de Sécurité Sanitaire French Agency for Food Environmental and Occupational Health Safety. Laboratory for Food Safety (Anses, 14 rue Pierre et Marie CurieSchool of Veterinary Medicine and Animal Science (FMVZ-UNESP)Agence Nationale de Sécurité Sanitaire Risk Assessment DepartmentPasteur InstituteSchool of Veterinary Medicine and Animal Science (FMVZ-UNESP)Environmental and Occupational Health Safety. Laboratory for Food Safety (AnsesUniversidade Estadual Paulista (UNESP)Risk Assessment DepartmentPasteur InstituteRosa Rodrigues de Souza, Carolina [UNESP]Bergis, HélèneNg, PatriciaGuillier, LaurentFélix, BenjaminLeclercq, AlexandreGnanou Besse, Nathalie2023-07-29T13:12:30Z2023-07-29T13:12:30Z2023-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.fm.2023.104303Food Microbiology, v. 114.1095-99980740-0020http://hdl.handle.net/11449/24730810.1016/j.fm.2023.1043032-s2.0-85158033896Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFood Microbiologyinfo:eu-repo/semantics/openAccess2023-07-29T13:12:30Zoai:repositorio.unesp.br:11449/247308Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:12:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Assessment of the relationship between the MLST genetic diversity of Listeria monocytogenes and growth under selective and non-selective conditions |
title |
Assessment of the relationship between the MLST genetic diversity of Listeria monocytogenes and growth under selective and non-selective conditions |
spellingShingle |
Assessment of the relationship between the MLST genetic diversity of Listeria monocytogenes and growth under selective and non-selective conditions Rosa Rodrigues de Souza, Carolina [UNESP] Clonal complex Growth Listeria monocytogenes MLST Selective enrichment |
title_short |
Assessment of the relationship between the MLST genetic diversity of Listeria monocytogenes and growth under selective and non-selective conditions |
title_full |
Assessment of the relationship between the MLST genetic diversity of Listeria monocytogenes and growth under selective and non-selective conditions |
title_fullStr |
Assessment of the relationship between the MLST genetic diversity of Listeria monocytogenes and growth under selective and non-selective conditions |
title_full_unstemmed |
Assessment of the relationship between the MLST genetic diversity of Listeria monocytogenes and growth under selective and non-selective conditions |
title_sort |
Assessment of the relationship between the MLST genetic diversity of Listeria monocytogenes and growth under selective and non-selective conditions |
author |
Rosa Rodrigues de Souza, Carolina [UNESP] |
author_facet |
Rosa Rodrigues de Souza, Carolina [UNESP] Bergis, Hélène Ng, Patricia Guillier, Laurent Félix, Benjamin Leclercq, Alexandre Gnanou Besse, Nathalie |
author_role |
author |
author2 |
Bergis, Hélène Ng, Patricia Guillier, Laurent Félix, Benjamin Leclercq, Alexandre Gnanou Besse, Nathalie |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Environmental and Occupational Health Safety. Laboratory for Food Safety (Anses Universidade Estadual Paulista (UNESP) Risk Assessment Department Pasteur Institute |
dc.contributor.author.fl_str_mv |
Rosa Rodrigues de Souza, Carolina [UNESP] Bergis, Hélène Ng, Patricia Guillier, Laurent Félix, Benjamin Leclercq, Alexandre Gnanou Besse, Nathalie |
dc.subject.por.fl_str_mv |
Clonal complex Growth Listeria monocytogenes MLST Selective enrichment |
topic |
Clonal complex Growth Listeria monocytogenes MLST Selective enrichment |
description |
Listeria monocytogenes can grow under stressful conditions and contaminate various food categories. Progresss in DNA sequencing-based identification methods, such as multi-locus sequence typing (MLST) now allow for more accurate characterization of pathogens. L. monocytogenes MLST genetic diversity is reflected by the different prevalence of the “clonal complexes” (CCs) in foods or infections. Better understanding of the growth potentials of L. monocytogenes is essential for quantitative risk assessment and efficient detection across CCs genetic diversity. Using optical density measurements taken with an automated spectrophotometer, we compared the maximal growth rate and lag phase of 39 strains from 13 different CCs and various food origins, in 3 broths mimicking stresful food conditions (8 °C, aw 0.95 and pH5) and in ISO Standard enrichment broths (Half Fraser and Fraser). This is important as growth could influence risk through pathogen multiplication in food. Besides, enrichment problems could lead to a lack of detection of some CCs. Despite small differences highlighting natural intraspecific variability, our results show that growth performances of L. monocytogenes strains under the conditions tested in selective and non-selective broth do not appear to be strongly correlated to CCs and cannot explain higher CC “virulence” or prevalence. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:12:30Z 2023-07-29T13:12:30Z 2023-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.fm.2023.104303 Food Microbiology, v. 114. 1095-9998 0740-0020 http://hdl.handle.net/11449/247308 10.1016/j.fm.2023.104303 2-s2.0-85158033896 |
url |
http://dx.doi.org/10.1016/j.fm.2023.104303 http://hdl.handle.net/11449/247308 |
identifier_str_mv |
Food Microbiology, v. 114. 1095-9998 0740-0020 10.1016/j.fm.2023.104303 2-s2.0-85158033896 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Food Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803047014910394368 |