Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/pathogens11020218 http://hdl.handle.net/11449/230370 |
Resumo: | (1) Background: Peritonitis due to nonfermenting Gram-negative bacilli (NF-GNB) is a dramatic complication of peritoneal dialysis (PD) with bad outcomes. Previous studies of PD-related peritonitis due to Pseudomonas species have shown a low-resolution rate, without a high resistance rate to antipseudomonal antibiotics. This suggests that bacterial virulence factors can act and influence peritonitis evolution. This study aimed to describe the microbiological characteristics of NF-GNB causing PD-related peritonitis and analyze their influence on the outcome. (2) Methods: We analyze the 48 isolates from NF-GNB peritonitis, which were stored in our culture collection regarding bacterial resistance, biofilm, and other virulence factors’ production, and clonal profile. Additionally, we collected data on treatment and outcomes from patients’ clinical registers. (3) Results: The etiologies were species of Pseudomonas (50%), Acinetobacter (36%), and other NF-GNB (14%). There was a high (75%) proportion of biofilm producer lineages. The in vitro susceptibility rate of Pseudomonas spp. to amikacin, ciprofloxacin, and ceftazidime was significantly greater than that of Acinetobacter spp. and other species; however, there was a similar low-resolution rate (<45%) among the episodes attributable to them. Pseudomonas species have a polyclonal profile, while we found a clone of five multiresistant Acinetobacter baumannii over an 8-year interval (2000–2008), which suggest an origin from the healthcare environment. (4) Conclusions: We are not able to identify any predictor of outcome, but it is possible that biofilm and others virulence factors can act in concert and contribute to the bad outcome. |
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Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis PatientsAcinetobacterAntimicrobial resistanceBiofilmClonal profileNonfermenting Gram-negative bacilliPeritoneal dialysisPeritonitisPseudomonasVirulence factors(1) Background: Peritonitis due to nonfermenting Gram-negative bacilli (NF-GNB) is a dramatic complication of peritoneal dialysis (PD) with bad outcomes. Previous studies of PD-related peritonitis due to Pseudomonas species have shown a low-resolution rate, without a high resistance rate to antipseudomonal antibiotics. This suggests that bacterial virulence factors can act and influence peritonitis evolution. This study aimed to describe the microbiological characteristics of NF-GNB causing PD-related peritonitis and analyze their influence on the outcome. (2) Methods: We analyze the 48 isolates from NF-GNB peritonitis, which were stored in our culture collection regarding bacterial resistance, biofilm, and other virulence factors’ production, and clonal profile. Additionally, we collected data on treatment and outcomes from patients’ clinical registers. (3) Results: The etiologies were species of Pseudomonas (50%), Acinetobacter (36%), and other NF-GNB (14%). There was a high (75%) proportion of biofilm producer lineages. The in vitro susceptibility rate of Pseudomonas spp. to amikacin, ciprofloxacin, and ceftazidime was significantly greater than that of Acinetobacter spp. and other species; however, there was a similar low-resolution rate (<45%) among the episodes attributable to them. Pseudomonas species have a polyclonal profile, while we found a clone of five multiresistant Acinetobacter baumannii over an 8-year interval (2000–2008), which suggest an origin from the healthcare environment. (4) Conclusions: We are not able to identify any predictor of outcome, but it is possible that biofilm and others virulence factors can act in concert and contribute to the bad outcome.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Ciências Químicas e Biológicas Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SPCentro de Bacteriologia Instituto Adolfo Lutz, SPHospital das Clínicas da Faculdade de Medicina de Botucatu, SPDepartamento de Clínica Médica Faculdade de Medicina Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SPDepartamento de Ciências Químicas e Biológicas Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SPHospital das Clínicas da Faculdade de Medicina de Botucatu, SPDepartamento de Clínica Médica Faculdade de Medicina Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SPFAPESP: 2013/21379-8Universidade Estadual Paulista (UNESP)Instituto Adolfo LutzMoro Lima Dos Santos, Ana Cláudia [UNESP]Monteiro, Aydir Cecília Marinho [UNESP]Barbosa, Thaís Alves [UNESP]Riboli, Danilo Flávio Moraes [UNESP]Camargo, Carlos HenriqueFerreira, Adriano Martison [UNESP]Mondelli, Alessandro Lia [UNESP]Montelli, Augusto Cezar [UNESP]Hernandes, Rodrigo Tavanelli [UNESP]Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]Barretti, Pasqual [UNESP]2022-04-29T08:39:30Z2022-04-29T08:39:30Z2022-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/pathogens11020218Pathogens, v. 11, n. 2, 2022.2076-0817http://hdl.handle.net/11449/23037010.3390/pathogens110202182-s2.0-85124426014Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPathogensinfo:eu-repo/semantics/openAccess2024-09-30T17:35:29Zoai:repositorio.unesp.br:11449/230370Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-30T17:35:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients |
title |
Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients |
spellingShingle |
Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients Moro Lima Dos Santos, Ana Cláudia [UNESP] Acinetobacter Antimicrobial resistance Biofilm Clonal profile Nonfermenting Gram-negative bacilli Peritoneal dialysis Peritonitis Pseudomonas Virulence factors |
title_short |
Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients |
title_full |
Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients |
title_fullStr |
Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients |
title_full_unstemmed |
Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients |
title_sort |
Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients |
author |
Moro Lima Dos Santos, Ana Cláudia [UNESP] |
author_facet |
Moro Lima Dos Santos, Ana Cláudia [UNESP] Monteiro, Aydir Cecília Marinho [UNESP] Barbosa, Thaís Alves [UNESP] Riboli, Danilo Flávio Moraes [UNESP] Camargo, Carlos Henrique Ferreira, Adriano Martison [UNESP] Mondelli, Alessandro Lia [UNESP] Montelli, Augusto Cezar [UNESP] Hernandes, Rodrigo Tavanelli [UNESP] Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP] Barretti, Pasqual [UNESP] |
author_role |
author |
author2 |
Monteiro, Aydir Cecília Marinho [UNESP] Barbosa, Thaís Alves [UNESP] Riboli, Danilo Flávio Moraes [UNESP] Camargo, Carlos Henrique Ferreira, Adriano Martison [UNESP] Mondelli, Alessandro Lia [UNESP] Montelli, Augusto Cezar [UNESP] Hernandes, Rodrigo Tavanelli [UNESP] Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP] Barretti, Pasqual [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Instituto Adolfo Lutz |
dc.contributor.author.fl_str_mv |
Moro Lima Dos Santos, Ana Cláudia [UNESP] Monteiro, Aydir Cecília Marinho [UNESP] Barbosa, Thaís Alves [UNESP] Riboli, Danilo Flávio Moraes [UNESP] Camargo, Carlos Henrique Ferreira, Adriano Martison [UNESP] Mondelli, Alessandro Lia [UNESP] Montelli, Augusto Cezar [UNESP] Hernandes, Rodrigo Tavanelli [UNESP] Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP] Barretti, Pasqual [UNESP] |
dc.subject.por.fl_str_mv |
Acinetobacter Antimicrobial resistance Biofilm Clonal profile Nonfermenting Gram-negative bacilli Peritoneal dialysis Peritonitis Pseudomonas Virulence factors |
topic |
Acinetobacter Antimicrobial resistance Biofilm Clonal profile Nonfermenting Gram-negative bacilli Peritoneal dialysis Peritonitis Pseudomonas Virulence factors |
description |
(1) Background: Peritonitis due to nonfermenting Gram-negative bacilli (NF-GNB) is a dramatic complication of peritoneal dialysis (PD) with bad outcomes. Previous studies of PD-related peritonitis due to Pseudomonas species have shown a low-resolution rate, without a high resistance rate to antipseudomonal antibiotics. This suggests that bacterial virulence factors can act and influence peritonitis evolution. This study aimed to describe the microbiological characteristics of NF-GNB causing PD-related peritonitis and analyze their influence on the outcome. (2) Methods: We analyze the 48 isolates from NF-GNB peritonitis, which were stored in our culture collection regarding bacterial resistance, biofilm, and other virulence factors’ production, and clonal profile. Additionally, we collected data on treatment and outcomes from patients’ clinical registers. (3) Results: The etiologies were species of Pseudomonas (50%), Acinetobacter (36%), and other NF-GNB (14%). There was a high (75%) proportion of biofilm producer lineages. The in vitro susceptibility rate of Pseudomonas spp. to amikacin, ciprofloxacin, and ceftazidime was significantly greater than that of Acinetobacter spp. and other species; however, there was a similar low-resolution rate (<45%) among the episodes attributable to them. Pseudomonas species have a polyclonal profile, while we found a clone of five multiresistant Acinetobacter baumannii over an 8-year interval (2000–2008), which suggest an origin from the healthcare environment. (4) Conclusions: We are not able to identify any predictor of outcome, but it is possible that biofilm and others virulence factors can act in concert and contribute to the bad outcome. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:39:30Z 2022-04-29T08:39:30Z 2022-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/pathogens11020218 Pathogens, v. 11, n. 2, 2022. 2076-0817 http://hdl.handle.net/11449/230370 10.3390/pathogens11020218 2-s2.0-85124426014 |
url |
http://dx.doi.org/10.3390/pathogens11020218 http://hdl.handle.net/11449/230370 |
identifier_str_mv |
Pathogens, v. 11, n. 2, 2022. 2076-0817 10.3390/pathogens11020218 2-s2.0-85124426014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Pathogens |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546482011209728 |