Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients

Detalhes bibliográficos
Autor(a) principal: Moro Lima Dos Santos, Ana Cláudia [UNESP]
Data de Publicação: 2022
Outros Autores: Monteiro, Aydir Cecília Marinho [UNESP], Barbosa, Thaís Alves [UNESP], Riboli, Danilo Flávio Moraes [UNESP], Camargo, Carlos Henrique, Ferreira, Adriano Martison [UNESP], Mondelli, Alessandro Lia [UNESP], Montelli, Augusto Cezar [UNESP], Hernandes, Rodrigo Tavanelli [UNESP], Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP], Barretti, Pasqual [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/pathogens11020218
http://hdl.handle.net/11449/230370
Resumo: (1) Background: Peritonitis due to nonfermenting Gram-negative bacilli (NF-GNB) is a dramatic complication of peritoneal dialysis (PD) with bad outcomes. Previous studies of PD-related peritonitis due to Pseudomonas species have shown a low-resolution rate, without a high resistance rate to antipseudomonal antibiotics. This suggests that bacterial virulence factors can act and influence peritonitis evolution. This study aimed to describe the microbiological characteristics of NF-GNB causing PD-related peritonitis and analyze their influence on the outcome. (2) Methods: We analyze the 48 isolates from NF-GNB peritonitis, which were stored in our culture collection regarding bacterial resistance, biofilm, and other virulence factors’ production, and clonal profile. Additionally, we collected data on treatment and outcomes from patients’ clinical registers. (3) Results: The etiologies were species of Pseudomonas (50%), Acinetobacter (36%), and other NF-GNB (14%). There was a high (75%) proportion of biofilm producer lineages. The in vitro susceptibility rate of Pseudomonas spp. to amikacin, ciprofloxacin, and ceftazidime was significantly greater than that of Acinetobacter spp. and other species; however, there was a similar low-resolution rate (<45%) among the episodes attributable to them. Pseudomonas species have a polyclonal profile, while we found a clone of five multiresistant Acinetobacter baumannii over an 8-year interval (2000–2008), which suggest an origin from the healthcare environment. (4) Conclusions: We are not able to identify any predictor of outcome, but it is possible that biofilm and others virulence factors can act in concert and contribute to the bad outcome.
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spelling Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis PatientsAcinetobacterAntimicrobial resistanceBiofilmClonal profileNonfermenting Gram-negative bacilliPeritoneal dialysisPeritonitisPseudomonasVirulence factors(1) Background: Peritonitis due to nonfermenting Gram-negative bacilli (NF-GNB) is a dramatic complication of peritoneal dialysis (PD) with bad outcomes. Previous studies of PD-related peritonitis due to Pseudomonas species have shown a low-resolution rate, without a high resistance rate to antipseudomonal antibiotics. This suggests that bacterial virulence factors can act and influence peritonitis evolution. This study aimed to describe the microbiological characteristics of NF-GNB causing PD-related peritonitis and analyze their influence on the outcome. (2) Methods: We analyze the 48 isolates from NF-GNB peritonitis, which were stored in our culture collection regarding bacterial resistance, biofilm, and other virulence factors’ production, and clonal profile. Additionally, we collected data on treatment and outcomes from patients’ clinical registers. (3) Results: The etiologies were species of Pseudomonas (50%), Acinetobacter (36%), and other NF-GNB (14%). There was a high (75%) proportion of biofilm producer lineages. The in vitro susceptibility rate of Pseudomonas spp. to amikacin, ciprofloxacin, and ceftazidime was significantly greater than that of Acinetobacter spp. and other species; however, there was a similar low-resolution rate (<45%) among the episodes attributable to them. Pseudomonas species have a polyclonal profile, while we found a clone of five multiresistant Acinetobacter baumannii over an 8-year interval (2000–2008), which suggest an origin from the healthcare environment. (4) Conclusions: We are not able to identify any predictor of outcome, but it is possible that biofilm and others virulence factors can act in concert and contribute to the bad outcome.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Ciências Químicas e Biológicas Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SPCentro de Bacteriologia Instituto Adolfo Lutz, SPHospital das Clínicas da Faculdade de Medicina de Botucatu, SPDepartamento de Clínica Médica Faculdade de Medicina Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SPDepartamento de Ciências Químicas e Biológicas Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SPHospital das Clínicas da Faculdade de Medicina de Botucatu, SPDepartamento de Clínica Médica Faculdade de Medicina Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SPFAPESP: 2013/21379-8Universidade Estadual Paulista (UNESP)Instituto Adolfo LutzMoro Lima Dos Santos, Ana Cláudia [UNESP]Monteiro, Aydir Cecília Marinho [UNESP]Barbosa, Thaís Alves [UNESP]Riboli, Danilo Flávio Moraes [UNESP]Camargo, Carlos HenriqueFerreira, Adriano Martison [UNESP]Mondelli, Alessandro Lia [UNESP]Montelli, Augusto Cezar [UNESP]Hernandes, Rodrigo Tavanelli [UNESP]Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]Barretti, Pasqual [UNESP]2022-04-29T08:39:30Z2022-04-29T08:39:30Z2022-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/pathogens11020218Pathogens, v. 11, n. 2, 2022.2076-0817http://hdl.handle.net/11449/23037010.3390/pathogens110202182-s2.0-85124426014Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPathogensinfo:eu-repo/semantics/openAccess2024-09-30T17:35:29Zoai:repositorio.unesp.br:11449/230370Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-30T17:35:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients
title Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients
spellingShingle Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients
Moro Lima Dos Santos, Ana Cláudia [UNESP]
Acinetobacter
Antimicrobial resistance
Biofilm
Clonal profile
Nonfermenting Gram-negative bacilli
Peritoneal dialysis
Peritonitis
Pseudomonas
Virulence factors
title_short Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients
title_full Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients
title_fullStr Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients
title_full_unstemmed Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients
title_sort Phenotypic and Molecular Characterization of Nonfermenting Gram-Negative Bacilli Causing Peritonitis in Peritoneal Dialysis Patients
author Moro Lima Dos Santos, Ana Cláudia [UNESP]
author_facet Moro Lima Dos Santos, Ana Cláudia [UNESP]
Monteiro, Aydir Cecília Marinho [UNESP]
Barbosa, Thaís Alves [UNESP]
Riboli, Danilo Flávio Moraes [UNESP]
Camargo, Carlos Henrique
Ferreira, Adriano Martison [UNESP]
Mondelli, Alessandro Lia [UNESP]
Montelli, Augusto Cezar [UNESP]
Hernandes, Rodrigo Tavanelli [UNESP]
Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]
Barretti, Pasqual [UNESP]
author_role author
author2 Monteiro, Aydir Cecília Marinho [UNESP]
Barbosa, Thaís Alves [UNESP]
Riboli, Danilo Flávio Moraes [UNESP]
Camargo, Carlos Henrique
Ferreira, Adriano Martison [UNESP]
Mondelli, Alessandro Lia [UNESP]
Montelli, Augusto Cezar [UNESP]
Hernandes, Rodrigo Tavanelli [UNESP]
Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]
Barretti, Pasqual [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Instituto Adolfo Lutz
dc.contributor.author.fl_str_mv Moro Lima Dos Santos, Ana Cláudia [UNESP]
Monteiro, Aydir Cecília Marinho [UNESP]
Barbosa, Thaís Alves [UNESP]
Riboli, Danilo Flávio Moraes [UNESP]
Camargo, Carlos Henrique
Ferreira, Adriano Martison [UNESP]
Mondelli, Alessandro Lia [UNESP]
Montelli, Augusto Cezar [UNESP]
Hernandes, Rodrigo Tavanelli [UNESP]
Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP]
Barretti, Pasqual [UNESP]
dc.subject.por.fl_str_mv Acinetobacter
Antimicrobial resistance
Biofilm
Clonal profile
Nonfermenting Gram-negative bacilli
Peritoneal dialysis
Peritonitis
Pseudomonas
Virulence factors
topic Acinetobacter
Antimicrobial resistance
Biofilm
Clonal profile
Nonfermenting Gram-negative bacilli
Peritoneal dialysis
Peritonitis
Pseudomonas
Virulence factors
description (1) Background: Peritonitis due to nonfermenting Gram-negative bacilli (NF-GNB) is a dramatic complication of peritoneal dialysis (PD) with bad outcomes. Previous studies of PD-related peritonitis due to Pseudomonas species have shown a low-resolution rate, without a high resistance rate to antipseudomonal antibiotics. This suggests that bacterial virulence factors can act and influence peritonitis evolution. This study aimed to describe the microbiological characteristics of NF-GNB causing PD-related peritonitis and analyze their influence on the outcome. (2) Methods: We analyze the 48 isolates from NF-GNB peritonitis, which were stored in our culture collection regarding bacterial resistance, biofilm, and other virulence factors’ production, and clonal profile. Additionally, we collected data on treatment and outcomes from patients’ clinical registers. (3) Results: The etiologies were species of Pseudomonas (50%), Acinetobacter (36%), and other NF-GNB (14%). There was a high (75%) proportion of biofilm producer lineages. The in vitro susceptibility rate of Pseudomonas spp. to amikacin, ciprofloxacin, and ceftazidime was significantly greater than that of Acinetobacter spp. and other species; however, there was a similar low-resolution rate (<45%) among the episodes attributable to them. Pseudomonas species have a polyclonal profile, while we found a clone of five multiresistant Acinetobacter baumannii over an 8-year interval (2000–2008), which suggest an origin from the healthcare environment. (4) Conclusions: We are not able to identify any predictor of outcome, but it is possible that biofilm and others virulence factors can act in concert and contribute to the bad outcome.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:39:30Z
2022-04-29T08:39:30Z
2022-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/pathogens11020218
Pathogens, v. 11, n. 2, 2022.
2076-0817
http://hdl.handle.net/11449/230370
10.3390/pathogens11020218
2-s2.0-85124426014
url http://dx.doi.org/10.3390/pathogens11020218
http://hdl.handle.net/11449/230370
identifier_str_mv Pathogens, v. 11, n. 2, 2022.
2076-0817
10.3390/pathogens11020218
2-s2.0-85124426014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pathogens
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
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instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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