Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle

Detalhes bibliográficos
Autor(a) principal: Franco, Marília Masello Junqueira [UNESP]
Data de Publicação: 2017
Outros Autores: Ribeiro, Márcio Garcia [UNESP], Pavan, Fernando Rogério [UNESP], Miyata, Marcelo [UNESP], Heinemann, Marcos Bryan, de Souza Filho, Antonio Francisco, Cardoso, Rosilene Fressatti, de Almeida, Aryadne Larissa, Sakate, Ricardo Ichiro, Paes, Antonio Carlos [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.tube.2017.02.006
http://hdl.handle.net/11449/174312
Resumo: In developing nations, 10–20% of the human cases of tuberculosis are caused by Mycobacterium bovis. However, this percentage may be underestimated because most laboratories in developing countries do not routinely perform mycobacterial cultures, and only a few have the systems in place to identify M. bovis. There are few studies investigating genotypic diversity and drug resistance in M. bovis from animal and/or human infections. The genotypic diversity of M. bovis strains obtained from bovine lymph nodes were investigated by spacer oligonucleotide typing (spoligotyping) and mycobacterial interspersed repetitive unit–variable-number tandem repeat typing (MIRU-VNTR). The phenotypic resistance to isoniazid and rifampicin and MIC values of the isolates were determined using the resazurin microtiter assay plate method (REMA). The evaluation of the possible genetic basis for such resistance was performed with GenoType MTBDRplus. Sixty-seven isolates were obtained, of which 11 (16%) were MDR-TB, 8 (12%) were isoniazid-resistant, and 2 (3%) were rifampicin-resistant. Mutations associated with drug resistance were not found. Genotyping techniques enabled the grouping of the strains into 12 clusters and 21 isolates with unique profiles. The high frequency of M. bovis reinforces the impact of the pathogen as a major causal agent of bovine tuberculosis in the study area. The resistance of the strains to drugs used for first-line treatment of human tuberculosis raises public health concerns. Further studies are required to elucidate the basis of drug resistance and genotypic diversity in M. bovis.
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spelling Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattleBovine tuberculosisMIRU-VNTRMultidrug-resistantMycobacterium bovisSpoligotypingIn developing nations, 10–20% of the human cases of tuberculosis are caused by Mycobacterium bovis. However, this percentage may be underestimated because most laboratories in developing countries do not routinely perform mycobacterial cultures, and only a few have the systems in place to identify M. bovis. There are few studies investigating genotypic diversity and drug resistance in M. bovis from animal and/or human infections. The genotypic diversity of M. bovis strains obtained from bovine lymph nodes were investigated by spacer oligonucleotide typing (spoligotyping) and mycobacterial interspersed repetitive unit–variable-number tandem repeat typing (MIRU-VNTR). The phenotypic resistance to isoniazid and rifampicin and MIC values of the isolates were determined using the resazurin microtiter assay plate method (REMA). The evaluation of the possible genetic basis for such resistance was performed with GenoType MTBDRplus. Sixty-seven isolates were obtained, of which 11 (16%) were MDR-TB, 8 (12%) were isoniazid-resistant, and 2 (3%) were rifampicin-resistant. Mutations associated with drug resistance were not found. Genotyping techniques enabled the grouping of the strains into 12 clusters and 21 isolates with unique profiles. The high frequency of M. bovis reinforces the impact of the pathogen as a major causal agent of bovine tuberculosis in the study area. The resistance of the strains to drugs used for first-line treatment of human tuberculosis raises public health concerns. Further studies are required to elucidate the basis of drug resistance and genotypic diversity in M. bovis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences UNESP-Univ. Estadual Paulista, P.O. Box: 560, Prof. Dr. Walter Mauricio Correa Street, s/n, Rubiao Junior districtLaboratory of Tuberculosis Research School of Pharmaceutical Sciences UNESP- Univ. Estadual Paulista, Araraquara-Jau, km 01Department of Preventive Veterinary Medicine and Animal Health USP-Univ. de Sao Paulo, Prof. Dr. Orlando Marques de Paiva Avenue, 87Department of Clinical Analysis and Biomedicine UEM-Univ. Estadual de Maringá, Colombo Avenue, 5790Federal Veterinary Inspection Service State of Sao Paulo, Dr. Gabriel de Oliveira Rocha Street, 704Department of Pathophysiology and Host Defense JATA-Japan Anti-tuberculosis Association RIT-Research Institute of Tuberculosis, Zip Code: 204-8533, 3-1-24 MatsuyamaDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences UNESP-Univ. Estadual Paulista, P.O. Box: 560, Prof. Dr. Walter Mauricio Correa Street, s/n, Rubiao Junior districtLaboratory of Tuberculosis Research School of Pharmaceutical Sciences UNESP- Univ. Estadual Paulista, Araraquara-Jau, km 01FAPESP: #2014/04929-7FAPESP: 2014/04929-7Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Universidade Estadual de Maringá (UEM)State of Sao PauloRIT-Research Institute of TuberculosisFranco, Marília Masello Junqueira [UNESP]Ribeiro, Márcio Garcia [UNESP]Pavan, Fernando Rogério [UNESP]Miyata, Marcelo [UNESP]Heinemann, Marcos Bryande Souza Filho, Antonio FranciscoCardoso, Rosilene Fressattide Almeida, Aryadne LarissaSakate, Ricardo IchiroPaes, Antonio Carlos [UNESP]2018-12-11T17:10:23Z2018-12-11T17:10:23Z2017-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article30-37application/pdfhttp://dx.doi.org/10.1016/j.tube.2017.02.006Tuberculosis, v. 104, p. 30-37.1873-281X1472-9792http://hdl.handle.net/11449/17431210.1016/j.tube.2017.02.0062-s2.0-850147677652-s2.0-85014767765.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTuberculosis1,264info:eu-repo/semantics/openAccess2024-09-03T14:11:32Zoai:repositorio.unesp.br:11449/174312Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T14:11:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle
title Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle
spellingShingle Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle
Franco, Marília Masello Junqueira [UNESP]
Bovine tuberculosis
MIRU-VNTR
Multidrug-resistant
Mycobacterium bovis
Spoligotyping
title_short Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle
title_full Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle
title_fullStr Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle
title_full_unstemmed Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle
title_sort Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle
author Franco, Marília Masello Junqueira [UNESP]
author_facet Franco, Marília Masello Junqueira [UNESP]
Ribeiro, Márcio Garcia [UNESP]
Pavan, Fernando Rogério [UNESP]
Miyata, Marcelo [UNESP]
Heinemann, Marcos Bryan
de Souza Filho, Antonio Francisco
Cardoso, Rosilene Fressatti
de Almeida, Aryadne Larissa
Sakate, Ricardo Ichiro
Paes, Antonio Carlos [UNESP]
author_role author
author2 Ribeiro, Márcio Garcia [UNESP]
Pavan, Fernando Rogério [UNESP]
Miyata, Marcelo [UNESP]
Heinemann, Marcos Bryan
de Souza Filho, Antonio Francisco
Cardoso, Rosilene Fressatti
de Almeida, Aryadne Larissa
Sakate, Ricardo Ichiro
Paes, Antonio Carlos [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
Universidade Estadual de Maringá (UEM)
State of Sao Paulo
RIT-Research Institute of Tuberculosis
dc.contributor.author.fl_str_mv Franco, Marília Masello Junqueira [UNESP]
Ribeiro, Márcio Garcia [UNESP]
Pavan, Fernando Rogério [UNESP]
Miyata, Marcelo [UNESP]
Heinemann, Marcos Bryan
de Souza Filho, Antonio Francisco
Cardoso, Rosilene Fressatti
de Almeida, Aryadne Larissa
Sakate, Ricardo Ichiro
Paes, Antonio Carlos [UNESP]
dc.subject.por.fl_str_mv Bovine tuberculosis
MIRU-VNTR
Multidrug-resistant
Mycobacterium bovis
Spoligotyping
topic Bovine tuberculosis
MIRU-VNTR
Multidrug-resistant
Mycobacterium bovis
Spoligotyping
description In developing nations, 10–20% of the human cases of tuberculosis are caused by Mycobacterium bovis. However, this percentage may be underestimated because most laboratories in developing countries do not routinely perform mycobacterial cultures, and only a few have the systems in place to identify M. bovis. There are few studies investigating genotypic diversity and drug resistance in M. bovis from animal and/or human infections. The genotypic diversity of M. bovis strains obtained from bovine lymph nodes were investigated by spacer oligonucleotide typing (spoligotyping) and mycobacterial interspersed repetitive unit–variable-number tandem repeat typing (MIRU-VNTR). The phenotypic resistance to isoniazid and rifampicin and MIC values of the isolates were determined using the resazurin microtiter assay plate method (REMA). The evaluation of the possible genetic basis for such resistance was performed with GenoType MTBDRplus. Sixty-seven isolates were obtained, of which 11 (16%) were MDR-TB, 8 (12%) were isoniazid-resistant, and 2 (3%) were rifampicin-resistant. Mutations associated with drug resistance were not found. Genotyping techniques enabled the grouping of the strains into 12 clusters and 21 isolates with unique profiles. The high frequency of M. bovis reinforces the impact of the pathogen as a major causal agent of bovine tuberculosis in the study area. The resistance of the strains to drugs used for first-line treatment of human tuberculosis raises public health concerns. Further studies are required to elucidate the basis of drug resistance and genotypic diversity in M. bovis.
publishDate 2017
dc.date.none.fl_str_mv 2017-05-01
2018-12-11T17:10:23Z
2018-12-11T17:10:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.tube.2017.02.006
Tuberculosis, v. 104, p. 30-37.
1873-281X
1472-9792
http://hdl.handle.net/11449/174312
10.1016/j.tube.2017.02.006
2-s2.0-85014767765
2-s2.0-85014767765.pdf
url http://dx.doi.org/10.1016/j.tube.2017.02.006
http://hdl.handle.net/11449/174312
identifier_str_mv Tuberculosis, v. 104, p. 30-37.
1873-281X
1472-9792
10.1016/j.tube.2017.02.006
2-s2.0-85014767765
2-s2.0-85014767765.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Tuberculosis
1,264
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 30-37
application/pdf
dc.source.none.fl_str_mv Scopus
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instname:Universidade Estadual Paulista (UNESP)
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instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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