Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.tube.2017.02.006 http://hdl.handle.net/11449/174312 |
Resumo: | In developing nations, 10–20% of the human cases of tuberculosis are caused by Mycobacterium bovis. However, this percentage may be underestimated because most laboratories in developing countries do not routinely perform mycobacterial cultures, and only a few have the systems in place to identify M. bovis. There are few studies investigating genotypic diversity and drug resistance in M. bovis from animal and/or human infections. The genotypic diversity of M. bovis strains obtained from bovine lymph nodes were investigated by spacer oligonucleotide typing (spoligotyping) and mycobacterial interspersed repetitive unit–variable-number tandem repeat typing (MIRU-VNTR). The phenotypic resistance to isoniazid and rifampicin and MIC values of the isolates were determined using the resazurin microtiter assay plate method (REMA). The evaluation of the possible genetic basis for such resistance was performed with GenoType MTBDRplus. Sixty-seven isolates were obtained, of which 11 (16%) were MDR-TB, 8 (12%) were isoniazid-resistant, and 2 (3%) were rifampicin-resistant. Mutations associated with drug resistance were not found. Genotyping techniques enabled the grouping of the strains into 12 clusters and 21 isolates with unique profiles. The high frequency of M. bovis reinforces the impact of the pathogen as a major causal agent of bovine tuberculosis in the study area. The resistance of the strains to drugs used for first-line treatment of human tuberculosis raises public health concerns. Further studies are required to elucidate the basis of drug resistance and genotypic diversity in M. bovis. |
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Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattleBovine tuberculosisMIRU-VNTRMultidrug-resistantMycobacterium bovisSpoligotypingIn developing nations, 10–20% of the human cases of tuberculosis are caused by Mycobacterium bovis. However, this percentage may be underestimated because most laboratories in developing countries do not routinely perform mycobacterial cultures, and only a few have the systems in place to identify M. bovis. There are few studies investigating genotypic diversity and drug resistance in M. bovis from animal and/or human infections. The genotypic diversity of M. bovis strains obtained from bovine lymph nodes were investigated by spacer oligonucleotide typing (spoligotyping) and mycobacterial interspersed repetitive unit–variable-number tandem repeat typing (MIRU-VNTR). The phenotypic resistance to isoniazid and rifampicin and MIC values of the isolates were determined using the resazurin microtiter assay plate method (REMA). The evaluation of the possible genetic basis for such resistance was performed with GenoType MTBDRplus. Sixty-seven isolates were obtained, of which 11 (16%) were MDR-TB, 8 (12%) were isoniazid-resistant, and 2 (3%) were rifampicin-resistant. Mutations associated with drug resistance were not found. Genotyping techniques enabled the grouping of the strains into 12 clusters and 21 isolates with unique profiles. The high frequency of M. bovis reinforces the impact of the pathogen as a major causal agent of bovine tuberculosis in the study area. The resistance of the strains to drugs used for first-line treatment of human tuberculosis raises public health concerns. Further studies are required to elucidate the basis of drug resistance and genotypic diversity in M. bovis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences UNESP-Univ. Estadual Paulista, P.O. Box: 560, Prof. Dr. Walter Mauricio Correa Street, s/n, Rubiao Junior districtLaboratory of Tuberculosis Research School of Pharmaceutical Sciences UNESP- Univ. Estadual Paulista, Araraquara-Jau, km 01Department of Preventive Veterinary Medicine and Animal Health USP-Univ. de Sao Paulo, Prof. Dr. Orlando Marques de Paiva Avenue, 87Department of Clinical Analysis and Biomedicine UEM-Univ. Estadual de Maringá, Colombo Avenue, 5790Federal Veterinary Inspection Service State of Sao Paulo, Dr. Gabriel de Oliveira Rocha Street, 704Department of Pathophysiology and Host Defense JATA-Japan Anti-tuberculosis Association RIT-Research Institute of Tuberculosis, Zip Code: 204-8533, 3-1-24 MatsuyamaDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences UNESP-Univ. Estadual Paulista, P.O. Box: 560, Prof. Dr. Walter Mauricio Correa Street, s/n, Rubiao Junior districtLaboratory of Tuberculosis Research School of Pharmaceutical Sciences UNESP- Univ. Estadual Paulista, Araraquara-Jau, km 01FAPESP: #2014/04929-7FAPESP: 2014/04929-7Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Universidade Estadual de Maringá (UEM)State of Sao PauloRIT-Research Institute of TuberculosisFranco, Marília Masello Junqueira [UNESP]Ribeiro, Márcio Garcia [UNESP]Pavan, Fernando Rogério [UNESP]Miyata, Marcelo [UNESP]Heinemann, Marcos Bryande Souza Filho, Antonio FranciscoCardoso, Rosilene Fressattide Almeida, Aryadne LarissaSakate, Ricardo IchiroPaes, Antonio Carlos [UNESP]2018-12-11T17:10:23Z2018-12-11T17:10:23Z2017-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article30-37application/pdfhttp://dx.doi.org/10.1016/j.tube.2017.02.006Tuberculosis, v. 104, p. 30-37.1873-281X1472-9792http://hdl.handle.net/11449/17431210.1016/j.tube.2017.02.0062-s2.0-850147677652-s2.0-85014767765.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTuberculosis1,264info:eu-repo/semantics/openAccess2024-09-03T14:11:32Zoai:repositorio.unesp.br:11449/174312Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T14:11:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle |
title |
Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle |
spellingShingle |
Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle Franco, Marília Masello Junqueira [UNESP] Bovine tuberculosis MIRU-VNTR Multidrug-resistant Mycobacterium bovis Spoligotyping |
title_short |
Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle |
title_full |
Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle |
title_fullStr |
Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle |
title_full_unstemmed |
Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle |
title_sort |
Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle |
author |
Franco, Marília Masello Junqueira [UNESP] |
author_facet |
Franco, Marília Masello Junqueira [UNESP] Ribeiro, Márcio Garcia [UNESP] Pavan, Fernando Rogério [UNESP] Miyata, Marcelo [UNESP] Heinemann, Marcos Bryan de Souza Filho, Antonio Francisco Cardoso, Rosilene Fressatti de Almeida, Aryadne Larissa Sakate, Ricardo Ichiro Paes, Antonio Carlos [UNESP] |
author_role |
author |
author2 |
Ribeiro, Márcio Garcia [UNESP] Pavan, Fernando Rogério [UNESP] Miyata, Marcelo [UNESP] Heinemann, Marcos Bryan de Souza Filho, Antonio Francisco Cardoso, Rosilene Fressatti de Almeida, Aryadne Larissa Sakate, Ricardo Ichiro Paes, Antonio Carlos [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Universidade Estadual de Maringá (UEM) State of Sao Paulo RIT-Research Institute of Tuberculosis |
dc.contributor.author.fl_str_mv |
Franco, Marília Masello Junqueira [UNESP] Ribeiro, Márcio Garcia [UNESP] Pavan, Fernando Rogério [UNESP] Miyata, Marcelo [UNESP] Heinemann, Marcos Bryan de Souza Filho, Antonio Francisco Cardoso, Rosilene Fressatti de Almeida, Aryadne Larissa Sakate, Ricardo Ichiro Paes, Antonio Carlos [UNESP] |
dc.subject.por.fl_str_mv |
Bovine tuberculosis MIRU-VNTR Multidrug-resistant Mycobacterium bovis Spoligotyping |
topic |
Bovine tuberculosis MIRU-VNTR Multidrug-resistant Mycobacterium bovis Spoligotyping |
description |
In developing nations, 10–20% of the human cases of tuberculosis are caused by Mycobacterium bovis. However, this percentage may be underestimated because most laboratories in developing countries do not routinely perform mycobacterial cultures, and only a few have the systems in place to identify M. bovis. There are few studies investigating genotypic diversity and drug resistance in M. bovis from animal and/or human infections. The genotypic diversity of M. bovis strains obtained from bovine lymph nodes were investigated by spacer oligonucleotide typing (spoligotyping) and mycobacterial interspersed repetitive unit–variable-number tandem repeat typing (MIRU-VNTR). The phenotypic resistance to isoniazid and rifampicin and MIC values of the isolates were determined using the resazurin microtiter assay plate method (REMA). The evaluation of the possible genetic basis for such resistance was performed with GenoType MTBDRplus. Sixty-seven isolates were obtained, of which 11 (16%) were MDR-TB, 8 (12%) were isoniazid-resistant, and 2 (3%) were rifampicin-resistant. Mutations associated with drug resistance were not found. Genotyping techniques enabled the grouping of the strains into 12 clusters and 21 isolates with unique profiles. The high frequency of M. bovis reinforces the impact of the pathogen as a major causal agent of bovine tuberculosis in the study area. The resistance of the strains to drugs used for first-line treatment of human tuberculosis raises public health concerns. Further studies are required to elucidate the basis of drug resistance and genotypic diversity in M. bovis. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-01 2018-12-11T17:10:23Z 2018-12-11T17:10:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.tube.2017.02.006 Tuberculosis, v. 104, p. 30-37. 1873-281X 1472-9792 http://hdl.handle.net/11449/174312 10.1016/j.tube.2017.02.006 2-s2.0-85014767765 2-s2.0-85014767765.pdf |
url |
http://dx.doi.org/10.1016/j.tube.2017.02.006 http://hdl.handle.net/11449/174312 |
identifier_str_mv |
Tuberculosis, v. 104, p. 30-37. 1873-281X 1472-9792 10.1016/j.tube.2017.02.006 2-s2.0-85014767765 2-s2.0-85014767765.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Tuberculosis 1,264 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
30-37 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021374577606656 |