Atividade antimicrobiana de nanopartículas de CeO2 in vitro frente às bactérias Escherichia coli e Staphylococcus aureus

• Autor(a) principal: Souza, Jacqueline Stella Barbosa de [UNESP]
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/155402; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-05-02/000882763.pdf
• topic: Nanopartículas; Óxidos de cério; Microondas; Agentes anti-infecciosos; Nanoparticles

Cerium oxide nanoparticles were obtained by the microwave-assisted hydrothermal synthesis technique and evaluated against the growth of Staphylococcus aureus and Escherichia coli species. Such interactions were monitored as a function of particle size of cerium oxide, growth medium, pH, surfactant and minimum inhibitory concentration. The goal of this study was to investigate the bactericidal properties of crystals of CeO2 synthesized from aqueous solutions of their salts and processed in hydrothermal system assisted by microwave radiation. The obtained crystals were characterized by X ray diffraction (DRX), Raman scattering spectroscopy, FT-IR, absorption spectroscopy, UV-Vis, field emission scanning electron microscopy (FEG-SEM) and transmission electron microscopy (TEM), specific surface area (BET). The antimicrobial activity of these crystals was evaluated against the bacteria Staphylococcus aureus and Escherichia coli, as well as the influence of the hexamethyltriethylamine (HMTA) and polyethylene glycol (PEG) surfactants on the structural, morphological and biological characteristics of these nanoparticles. The microwave-assisted hydrothermal synthesis (HAM) method has been shown to be an efficient synthesis method for obtaining nanoparticles, allowing the use of low synthesis temperatures (100ºC) and low processing time. The results indicate the formation of nanophos with cubic structure, space group Fm3m, monodisperse particles with band gap around 3.0 eV. Data indicate that the nanoparticles obtained are free of contaminants and that the growth mechanism of these in the hydrothermal reactor corresponds to the dissolution-precipitation process. The microbiological assays indicated the inhibition of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) when placed in front of the colloidal solution of 1000 ppm, ... (Complete abstract click electronic access below)