Corneal angiogenesis based on different protocols of alkaline cauterization in murine models

Detalhes bibliográficos
Autor(a) principal: Claros-Chacaltana, Flor Diana Yokoay [UNESP]
Data de Publicação: 2017
Outros Autores: Kobashigawa, Karina Kamachi [UNESP], Padua, Ivan Ricardo Martinez [UNESP], Valdetaro, Gisele Pereira [UNESP], Aldrovani, Marcela [UNESP], Laus, José Luiz [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/s0102-865020170080000002
http://hdl.handle.net/11449/179172
Resumo: Purpose: To establish and compare protocols of alkaline cauterization for inducing corneal angiogenesis in murine models. Methods: Twenty-four adult Wistar rats were distributed into four groups (G1, G2, G3, and G4). The right eye cornea from each rat was cauterized using filter paper (3 mm), soaked in a solution of silver and potassium nitrates (3:1). Cauterization times were 10 (G1 and G4), or 20 seconds (G2 and G3). Cauterized corneas were washed with Ringer’s lactate solution. The filter paper was either removed before washing (G1 and G2), or kept on the corneas (G3 and G4). Corneas were photographed at multiple time points (2, 4, 6, 8, 11, 13, and 15 days after the procedure), and neovascularization parameters were assayed. Results: Neovascularization was observed in 66% of G1 corneas, and 100% of G2, G3, and G4 corneas. On day 15, G1 corneas showed smaller vascularized areas (12.63 ± 12.59%) compared to those in the G3 (41.95 ± 17.32%) and G4 (33 ± 11.74%) (P < 0.05) groups. Conclusions: The silver and potassium nitrate solution effectively induced corneal angiogenesis. The G2, G3, and G4 protocols showed excellent reproducibility, and induced vascularization in 100% of corneas.
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spelling Corneal angiogenesis based on different protocols of alkaline cauterization in murine modelsCauteryCorneal neovascularizationRatsSilverPurpose: To establish and compare protocols of alkaline cauterization for inducing corneal angiogenesis in murine models. Methods: Twenty-four adult Wistar rats were distributed into four groups (G1, G2, G3, and G4). The right eye cornea from each rat was cauterized using filter paper (3 mm), soaked in a solution of silver and potassium nitrates (3:1). Cauterization times were 10 (G1 and G4), or 20 seconds (G2 and G3). Cauterized corneas were washed with Ringer’s lactate solution. The filter paper was either removed before washing (G1 and G2), or kept on the corneas (G3 and G4). Corneas were photographed at multiple time points (2, 4, 6, 8, 11, 13, and 15 days after the procedure), and neovascularization parameters were assayed. Results: Neovascularization was observed in 66% of G1 corneas, and 100% of G2, G3, and G4 corneas. On day 15, G1 corneas showed smaller vascularized areas (12.63 ± 12.59%) compared to those in the G3 (41.95 ± 17.32%) and G4 (33 ± 11.74%) (P < 0.05) groups. Conclusions: The silver and potassium nitrate solution effectively induced corneal angiogenesis. The G2, G3, and G4 protocols showed excellent reproducibility, and induced vascularization in 100% of corneas.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Ophthalmology Unit Department of Veterinary Clinical Medicine and Surgery Faculty of Agrarian and Veterinary Sciences Universidade Esatdual de São Paulo FCAV-UNESPOphthalmology Unit Department of Veterinary Clinical Medicine and Surgery Faculty of Agrarian and Veterinary Sciences Universidade Esatdual de São Paulo FCAV-UNESPFAPESP: 2009/51773-4CNPq: 300833/2010-5Universidade Estadual Paulista (Unesp)Claros-Chacaltana, Flor Diana Yokoay [UNESP]Kobashigawa, Karina Kamachi [UNESP]Padua, Ivan Ricardo Martinez [UNESP]Valdetaro, Gisele Pereira [UNESP]Aldrovani, Marcela [UNESP]Laus, José Luiz [UNESP]2018-12-11T17:34:04Z2018-12-11T17:34:04Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article607-616application/pdfhttp://dx.doi.org/10.1590/s0102-865020170080000002Acta Cirurgica Brasileira, v. 32, n. 8, p. 607-616, 2017.1678-26740102-8650http://hdl.handle.net/11449/17917210.1590/s0102-865020170080000002S0102-865020170008006072-s2.0-85029455855S0102-86502017000800607.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cirurgica Brasileira0,395info:eu-repo/semantics/openAccess2024-06-06T14:09:56Zoai:repositorio.unesp.br:11449/179172Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:53:34.556536Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Corneal angiogenesis based on different protocols of alkaline cauterization in murine models
title Corneal angiogenesis based on different protocols of alkaline cauterization in murine models
spellingShingle Corneal angiogenesis based on different protocols of alkaline cauterization in murine models
Claros-Chacaltana, Flor Diana Yokoay [UNESP]
Cautery
Corneal neovascularization
Rats
Silver
title_short Corneal angiogenesis based on different protocols of alkaline cauterization in murine models
title_full Corneal angiogenesis based on different protocols of alkaline cauterization in murine models
title_fullStr Corneal angiogenesis based on different protocols of alkaline cauterization in murine models
title_full_unstemmed Corneal angiogenesis based on different protocols of alkaline cauterization in murine models
title_sort Corneal angiogenesis based on different protocols of alkaline cauterization in murine models
author Claros-Chacaltana, Flor Diana Yokoay [UNESP]
author_facet Claros-Chacaltana, Flor Diana Yokoay [UNESP]
Kobashigawa, Karina Kamachi [UNESP]
Padua, Ivan Ricardo Martinez [UNESP]
Valdetaro, Gisele Pereira [UNESP]
Aldrovani, Marcela [UNESP]
Laus, José Luiz [UNESP]
author_role author
author2 Kobashigawa, Karina Kamachi [UNESP]
Padua, Ivan Ricardo Martinez [UNESP]
Valdetaro, Gisele Pereira [UNESP]
Aldrovani, Marcela [UNESP]
Laus, José Luiz [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Claros-Chacaltana, Flor Diana Yokoay [UNESP]
Kobashigawa, Karina Kamachi [UNESP]
Padua, Ivan Ricardo Martinez [UNESP]
Valdetaro, Gisele Pereira [UNESP]
Aldrovani, Marcela [UNESP]
Laus, José Luiz [UNESP]
dc.subject.por.fl_str_mv Cautery
Corneal neovascularization
Rats
Silver
topic Cautery
Corneal neovascularization
Rats
Silver
description Purpose: To establish and compare protocols of alkaline cauterization for inducing corneal angiogenesis in murine models. Methods: Twenty-four adult Wistar rats were distributed into four groups (G1, G2, G3, and G4). The right eye cornea from each rat was cauterized using filter paper (3 mm), soaked in a solution of silver and potassium nitrates (3:1). Cauterization times were 10 (G1 and G4), or 20 seconds (G2 and G3). Cauterized corneas were washed with Ringer’s lactate solution. The filter paper was either removed before washing (G1 and G2), or kept on the corneas (G3 and G4). Corneas were photographed at multiple time points (2, 4, 6, 8, 11, 13, and 15 days after the procedure), and neovascularization parameters were assayed. Results: Neovascularization was observed in 66% of G1 corneas, and 100% of G2, G3, and G4 corneas. On day 15, G1 corneas showed smaller vascularized areas (12.63 ± 12.59%) compared to those in the G3 (41.95 ± 17.32%) and G4 (33 ± 11.74%) (P < 0.05) groups. Conclusions: The silver and potassium nitrate solution effectively induced corneal angiogenesis. The G2, G3, and G4 protocols showed excellent reproducibility, and induced vascularization in 100% of corneas.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
2018-12-11T17:34:04Z
2018-12-11T17:34:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/s0102-865020170080000002
Acta Cirurgica Brasileira, v. 32, n. 8, p. 607-616, 2017.
1678-2674
0102-8650
http://hdl.handle.net/11449/179172
10.1590/s0102-865020170080000002
S0102-86502017000800607
2-s2.0-85029455855
S0102-86502017000800607.pdf
url http://dx.doi.org/10.1590/s0102-865020170080000002
http://hdl.handle.net/11449/179172
identifier_str_mv Acta Cirurgica Brasileira, v. 32, n. 8, p. 607-616, 2017.
1678-2674
0102-8650
10.1590/s0102-865020170080000002
S0102-86502017000800607
2-s2.0-85029455855
S0102-86502017000800607.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
0,395
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 607-616
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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