Corneal angiogenesis based on different protocols of alkaline cauterization in murine models
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/s0102-865020170080000002 http://hdl.handle.net/11449/179172 |
Resumo: | Purpose: To establish and compare protocols of alkaline cauterization for inducing corneal angiogenesis in murine models. Methods: Twenty-four adult Wistar rats were distributed into four groups (G1, G2, G3, and G4). The right eye cornea from each rat was cauterized using filter paper (3 mm), soaked in a solution of silver and potassium nitrates (3:1). Cauterization times were 10 (G1 and G4), or 20 seconds (G2 and G3). Cauterized corneas were washed with Ringer’s lactate solution. The filter paper was either removed before washing (G1 and G2), or kept on the corneas (G3 and G4). Corneas were photographed at multiple time points (2, 4, 6, 8, 11, 13, and 15 days after the procedure), and neovascularization parameters were assayed. Results: Neovascularization was observed in 66% of G1 corneas, and 100% of G2, G3, and G4 corneas. On day 15, G1 corneas showed smaller vascularized areas (12.63 ± 12.59%) compared to those in the G3 (41.95 ± 17.32%) and G4 (33 ± 11.74%) (P < 0.05) groups. Conclusions: The silver and potassium nitrate solution effectively induced corneal angiogenesis. The G2, G3, and G4 protocols showed excellent reproducibility, and induced vascularization in 100% of corneas. |
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Corneal angiogenesis based on different protocols of alkaline cauterization in murine modelsCauteryCorneal neovascularizationRatsSilverPurpose: To establish and compare protocols of alkaline cauterization for inducing corneal angiogenesis in murine models. Methods: Twenty-four adult Wistar rats were distributed into four groups (G1, G2, G3, and G4). The right eye cornea from each rat was cauterized using filter paper (3 mm), soaked in a solution of silver and potassium nitrates (3:1). Cauterization times were 10 (G1 and G4), or 20 seconds (G2 and G3). Cauterized corneas were washed with Ringer’s lactate solution. The filter paper was either removed before washing (G1 and G2), or kept on the corneas (G3 and G4). Corneas were photographed at multiple time points (2, 4, 6, 8, 11, 13, and 15 days after the procedure), and neovascularization parameters were assayed. Results: Neovascularization was observed in 66% of G1 corneas, and 100% of G2, G3, and G4 corneas. On day 15, G1 corneas showed smaller vascularized areas (12.63 ± 12.59%) compared to those in the G3 (41.95 ± 17.32%) and G4 (33 ± 11.74%) (P < 0.05) groups. Conclusions: The silver and potassium nitrate solution effectively induced corneal angiogenesis. The G2, G3, and G4 protocols showed excellent reproducibility, and induced vascularization in 100% of corneas.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Ophthalmology Unit Department of Veterinary Clinical Medicine and Surgery Faculty of Agrarian and Veterinary Sciences Universidade Esatdual de São Paulo FCAV-UNESPOphthalmology Unit Department of Veterinary Clinical Medicine and Surgery Faculty of Agrarian and Veterinary Sciences Universidade Esatdual de São Paulo FCAV-UNESPFAPESP: 2009/51773-4CNPq: 300833/2010-5Universidade Estadual Paulista (Unesp)Claros-Chacaltana, Flor Diana Yokoay [UNESP]Kobashigawa, Karina Kamachi [UNESP]Padua, Ivan Ricardo Martinez [UNESP]Valdetaro, Gisele Pereira [UNESP]Aldrovani, Marcela [UNESP]Laus, José Luiz [UNESP]2018-12-11T17:34:04Z2018-12-11T17:34:04Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article607-616application/pdfhttp://dx.doi.org/10.1590/s0102-865020170080000002Acta Cirurgica Brasileira, v. 32, n. 8, p. 607-616, 2017.1678-26740102-8650http://hdl.handle.net/11449/17917210.1590/s0102-865020170080000002S0102-865020170008006072-s2.0-85029455855S0102-86502017000800607.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cirurgica Brasileira0,395info:eu-repo/semantics/openAccess2024-06-06T14:09:56Zoai:repositorio.unesp.br:11449/179172Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:53:34.556536Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Corneal angiogenesis based on different protocols of alkaline cauterization in murine models |
title |
Corneal angiogenesis based on different protocols of alkaline cauterization in murine models |
spellingShingle |
Corneal angiogenesis based on different protocols of alkaline cauterization in murine models Claros-Chacaltana, Flor Diana Yokoay [UNESP] Cautery Corneal neovascularization Rats Silver |
title_short |
Corneal angiogenesis based on different protocols of alkaline cauterization in murine models |
title_full |
Corneal angiogenesis based on different protocols of alkaline cauterization in murine models |
title_fullStr |
Corneal angiogenesis based on different protocols of alkaline cauterization in murine models |
title_full_unstemmed |
Corneal angiogenesis based on different protocols of alkaline cauterization in murine models |
title_sort |
Corneal angiogenesis based on different protocols of alkaline cauterization in murine models |
author |
Claros-Chacaltana, Flor Diana Yokoay [UNESP] |
author_facet |
Claros-Chacaltana, Flor Diana Yokoay [UNESP] Kobashigawa, Karina Kamachi [UNESP] Padua, Ivan Ricardo Martinez [UNESP] Valdetaro, Gisele Pereira [UNESP] Aldrovani, Marcela [UNESP] Laus, José Luiz [UNESP] |
author_role |
author |
author2 |
Kobashigawa, Karina Kamachi [UNESP] Padua, Ivan Ricardo Martinez [UNESP] Valdetaro, Gisele Pereira [UNESP] Aldrovani, Marcela [UNESP] Laus, José Luiz [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Claros-Chacaltana, Flor Diana Yokoay [UNESP] Kobashigawa, Karina Kamachi [UNESP] Padua, Ivan Ricardo Martinez [UNESP] Valdetaro, Gisele Pereira [UNESP] Aldrovani, Marcela [UNESP] Laus, José Luiz [UNESP] |
dc.subject.por.fl_str_mv |
Cautery Corneal neovascularization Rats Silver |
topic |
Cautery Corneal neovascularization Rats Silver |
description |
Purpose: To establish and compare protocols of alkaline cauterization for inducing corneal angiogenesis in murine models. Methods: Twenty-four adult Wistar rats were distributed into four groups (G1, G2, G3, and G4). The right eye cornea from each rat was cauterized using filter paper (3 mm), soaked in a solution of silver and potassium nitrates (3:1). Cauterization times were 10 (G1 and G4), or 20 seconds (G2 and G3). Cauterized corneas were washed with Ringer’s lactate solution. The filter paper was either removed before washing (G1 and G2), or kept on the corneas (G3 and G4). Corneas were photographed at multiple time points (2, 4, 6, 8, 11, 13, and 15 days after the procedure), and neovascularization parameters were assayed. Results: Neovascularization was observed in 66% of G1 corneas, and 100% of G2, G3, and G4 corneas. On day 15, G1 corneas showed smaller vascularized areas (12.63 ± 12.59%) compared to those in the G3 (41.95 ± 17.32%) and G4 (33 ± 11.74%) (P < 0.05) groups. Conclusions: The silver and potassium nitrate solution effectively induced corneal angiogenesis. The G2, G3, and G4 protocols showed excellent reproducibility, and induced vascularization in 100% of corneas. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:34:04Z 2018-12-11T17:34:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/s0102-865020170080000002 Acta Cirurgica Brasileira, v. 32, n. 8, p. 607-616, 2017. 1678-2674 0102-8650 http://hdl.handle.net/11449/179172 10.1590/s0102-865020170080000002 S0102-86502017000800607 2-s2.0-85029455855 S0102-86502017000800607.pdf |
url |
http://dx.doi.org/10.1590/s0102-865020170080000002 http://hdl.handle.net/11449/179172 |
identifier_str_mv |
Acta Cirurgica Brasileira, v. 32, n. 8, p. 607-616, 2017. 1678-2674 0102-8650 10.1590/s0102-865020170080000002 S0102-86502017000800607 2-s2.0-85029455855 S0102-86502017000800607.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Cirurgica Brasileira 0,395 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
607-616 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128873134555136 |