The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model

Detalhes bibliográficos
Autor(a) principal: Mello, Glauco Reggiani
Data de Publicação: 2011
Outros Autores: Pizzolatti, Marcos Longo, Wasilewski, Daniel, Santhiago, Marcony R., Budel, Vinícius, Moreira, Hamilton
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/19273
Resumo: PURPOSE: To evaluate the use of subconjunctival bevacizumab on corneal neovascularization in an experimental rabbit model for its effect on vessel extension, inflammation, and corneal epithelialization. METHODS: In this prospective, randomized, blinded, experimental study, 20 rabbits were submitted to a chemical trauma with sodium hydroxide and subsequently divided into two groups. The experimental group received a subconjunctival injection of bevacizumab (0.15 m; 3.75 mg), and the control group received an injection of 0.15 ml saline solution. After 14 days, two blinded digital photograph analyses were conducted to evaluate the inflammation/diameter of the vessels according to pre-established criteria. A histopathological analysis of the cornea evaluated the state of the epithelium and the number of polymorphonuclear cells. RESULTS: A concordance analysis using Kappa's statistic showed a satisfactory level of agreement between the two blinded digital photography analyses. The neovascular vessel length was greater in the control group (p
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spelling The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model Antiangiogenic drugsCorneal neovascularizationCorneaNeovascularizationPathologyEye burns PURPOSE: To evaluate the use of subconjunctival bevacizumab on corneal neovascularization in an experimental rabbit model for its effect on vessel extension, inflammation, and corneal epithelialization. METHODS: In this prospective, randomized, blinded, experimental study, 20 rabbits were submitted to a chemical trauma with sodium hydroxide and subsequently divided into two groups. The experimental group received a subconjunctival injection of bevacizumab (0.15 m; 3.75 mg), and the control group received an injection of 0.15 ml saline solution. After 14 days, two blinded digital photograph analyses were conducted to evaluate the inflammation/diameter of the vessels according to pre-established criteria. A histopathological analysis of the cornea evaluated the state of the epithelium and the number of polymorphonuclear cells. RESULTS: A concordance analysis using Kappa's statistic showed a satisfactory level of agreement between the two blinded digital photography analyses. The neovascular vessel length was greater in the control group (pHospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1927310.1590/S1807-59322011000800023Clinics; v. 66 n. 8 (2011); 1443-1450 Clinics; Vol. 66 Núm. 8 (2011); 1443-1450 Clinics; Vol. 66 No. 8 (2011); 1443-1450 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/19273/21336Mello, Glauco ReggianiPizzolatti, Marcos LongoWasilewski, DanielSanthiago, Marcony R.Budel, ViníciusMoreira, Hamiltoninfo:eu-repo/semantics/openAccess2012-05-23T16:31:29Zoai:revistas.usp.br:article/19273Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-23T16:31:29Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model
title The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model
spellingShingle The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model
Mello, Glauco Reggiani
Antiangiogenic drugs
Corneal neovascularization
Cornea
Neovascularization
Pathology
Eye burns
title_short The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model
title_full The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model
title_fullStr The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model
title_full_unstemmed The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model
title_sort The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model
author Mello, Glauco Reggiani
author_facet Mello, Glauco Reggiani
Pizzolatti, Marcos Longo
Wasilewski, Daniel
Santhiago, Marcony R.
Budel, Vinícius
Moreira, Hamilton
author_role author
author2 Pizzolatti, Marcos Longo
Wasilewski, Daniel
Santhiago, Marcony R.
Budel, Vinícius
Moreira, Hamilton
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Mello, Glauco Reggiani
Pizzolatti, Marcos Longo
Wasilewski, Daniel
Santhiago, Marcony R.
Budel, Vinícius
Moreira, Hamilton
dc.subject.por.fl_str_mv Antiangiogenic drugs
Corneal neovascularization
Cornea
Neovascularization
Pathology
Eye burns
topic Antiangiogenic drugs
Corneal neovascularization
Cornea
Neovascularization
Pathology
Eye burns
description PURPOSE: To evaluate the use of subconjunctival bevacizumab on corneal neovascularization in an experimental rabbit model for its effect on vessel extension, inflammation, and corneal epithelialization. METHODS: In this prospective, randomized, blinded, experimental study, 20 rabbits were submitted to a chemical trauma with sodium hydroxide and subsequently divided into two groups. The experimental group received a subconjunctival injection of bevacizumab (0.15 m; 3.75 mg), and the control group received an injection of 0.15 ml saline solution. After 14 days, two blinded digital photograph analyses were conducted to evaluate the inflammation/diameter of the vessels according to pre-established criteria. A histopathological analysis of the cornea evaluated the state of the epithelium and the number of polymorphonuclear cells. RESULTS: A concordance analysis using Kappa's statistic showed a satisfactory level of agreement between the two blinded digital photography analyses. The neovascular vessel length was greater in the control group (p
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/19273
10.1590/S1807-59322011000800023
url https://www.revistas.usp.br/clinics/article/view/19273
identifier_str_mv 10.1590/S1807-59322011000800023
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/19273/21336
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; v. 66 n. 8 (2011); 1443-1450
Clinics; Vol. 66 Núm. 8 (2011); 1443-1450
Clinics; Vol. 66 No. 8 (2011); 1443-1450
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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