Cytogenotoxic evaluation of the acetonitrile extract, citrinin and dicitrinin-A from Penicillium citrinum

• Autor(a) principal: de Oliveira Filho, José Williams Gomes
• Data de Publicação: 2020
• Outros Autores: Andrade, Teresinha de Jesus Aguiar dos Santos, de Lima, Rosália Maria Tôrres, Silva, Dulce Helena Siqueira [UNESP], dos Reis, Antonielly Campinho, Santos, José Victor de Oliveira, de Meneses, Ag-Anne Pereira Melo, de Carvalho, Ricardo Melo, da Mata, Ana Maria Oliveira, de Alencar, Marcus Vinícius Oliveira Barros, Dias, Ana Carolina Soares, da Silva, Felipe Cavalcanti Carneiro, Islam, Muhammad Torequl, Clark, Cain C. T., Sousa, João Marcelo de Castro e, Melo-Cavalcante, Ana Amélia de Carvalho
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1080/01480545.2020.1769642; http://hdl.handle.net/11449/200574
• topic: cytotoxicity; Endophytic fungi; genotoxicity; mutagenicity; mycotoxins; Penicillium citrinum

Endophytic fungi are promising sources of bioactive substances; however, their secondary metabolites are toxic to plants, animals, and humans. This study aimed toevaluate the toxic, cytotoxic, mutagenic and oxidant/antioxidant activities of acetonitrile extract (AEPc), citrinin (CIT) and dicitrinin-A (DIC-A) of Penicillium citrinum. For this, the test substances at 0.5; 1.0; 1.5 and 2 μg/mLwere exposed for 24 and 48 h in Artemia salina, and 48 h in Allium cepa test systems. The oxidant/antioxidant test was evaluated in pre-, co- and post-treatment with the stressor hydrogen peroxide (H2O2) in Saccharomyces cerevisiae. The results suggest that the AEPc, CIT and DIC-A at 0.5; 1.0; 1.5 and 2 μg/mL showed toxicity in A. saline, with LC50 (24 h) of 2.03 μg/mL, 1.71 μg/mL and 2.29 μg/mL, and LC50 (48 h) of 0.51 μg/mL, 0.54 μg/mL and 0.54 μg/mL, respectively.In A. cepa, the test substances also exerted cytotoxic and mutagenic effects. The AEPc, CIT and DIC-A at lower concentrations modulated the damage induced by H2O2 in the proficient and mutant strains of S. cerevisiae for cytoplasmic and mitochondrial superoxide dismutase. Moreover, the AEPc at 2 μg/mL and CIT at the two highest concentrations did not affect the H2O2-induced DNA damage in the test strains. In conclusion, AEPc, CIT and DIC-A of P. citrinum may exert their toxic, cytotoxic and mutagenic effects in the test systems possibly through oxidative stress induction pathway.