Lethal salinity and survival for Macrobrachium carcinus larvae subjected to osmotic stress
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ecss.2021.107602 http://hdl.handle.net/11449/229591 |
Resumo: | The present study aimed to establish an adequate salinity range for the larviculture of the Macrobrachium carcinus by determining the lethal salinity (LS50) and survival of the larvae subjected to osmotic stress tests. Newly hatched larvae were acclimated at the salinities of 16 and 20 and reared for 20–22 days until reaching Zoea stage VI (ZVI). The larvae were then subjected to two osmotic stress tests carried out in 2-L plastic recipients (1–1.6L used volume) with a density of 50 larvae/L and with constant aeration. Each salinity had three replicates. Survival was recorded every 24 h after exposure to the salinities. In Test 1, ZVI larvae were transferred without acclimatization from salinity 16 to 0, 4, 8, 12, 16 (control), 20, 24 and 28 for 96 h. In Test 2, ZVI larvae were transferred without acclimatization from salinity 20 to 0, 4, 8, 12, 16, 20 (control), 24, 28, 32, 36 and 40 for 120 h. In both tests, 100% mortality of ZVI M. carcinus larvae was observed during the first hours upon altering the salinity to 0 and 4. Total mortality occurred at 72 h in the salinity of 40. In Test 1 (96 h), the best survival was shown with salinities between 16 and 24, with a maximum of 46.7% in 20 and 24. In Test 2 (120 h), the best survival was observed in salinities between 16 and 32, with a maximum of 71.3% in 28. The linear model generated from both tests (R2 = 92.8%) indicated that survival is optimized by using a salinity between 16 and 28. The LS50 (120 h) was estimated at 11.58 in the lower range and 33.70 in the upper range. In general, M. carcinus larvae in the Pre-culture phase (Zoea stages I to VI) of the larviculture showed more resistance to osmotic stress when reared in brackishwater with a salinity of 20 when compared with the salinity of 16, and the salinity range of 16–28 was the most adequate for the cultivation of ZVI M. carcinus larvae. |
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Lethal salinity and survival for Macrobrachium carcinus larvae subjected to osmotic stressAquacultureConservationFreshwater prawnsLarvaeLethal salinityLogistic modelThe present study aimed to establish an adequate salinity range for the larviculture of the Macrobrachium carcinus by determining the lethal salinity (LS50) and survival of the larvae subjected to osmotic stress tests. Newly hatched larvae were acclimated at the salinities of 16 and 20 and reared for 20–22 days until reaching Zoea stage VI (ZVI). The larvae were then subjected to two osmotic stress tests carried out in 2-L plastic recipients (1–1.6L used volume) with a density of 50 larvae/L and with constant aeration. Each salinity had three replicates. Survival was recorded every 24 h after exposure to the salinities. In Test 1, ZVI larvae were transferred without acclimatization from salinity 16 to 0, 4, 8, 12, 16 (control), 20, 24 and 28 for 96 h. In Test 2, ZVI larvae were transferred without acclimatization from salinity 20 to 0, 4, 8, 12, 16, 20 (control), 24, 28, 32, 36 and 40 for 120 h. In both tests, 100% mortality of ZVI M. carcinus larvae was observed during the first hours upon altering the salinity to 0 and 4. Total mortality occurred at 72 h in the salinity of 40. In Test 1 (96 h), the best survival was shown with salinities between 16 and 24, with a maximum of 46.7% in 20 and 24. In Test 2 (120 h), the best survival was observed in salinities between 16 and 32, with a maximum of 71.3% in 28. The linear model generated from both tests (R2 = 92.8%) indicated that survival is optimized by using a salinity between 16 and 28. The LS50 (120 h) was estimated at 11.58 in the lower range and 33.70 in the upper range. In general, M. carcinus larvae in the Pre-culture phase (Zoea stages I to VI) of the larviculture showed more resistance to osmotic stress when reared in brackishwater with a salinity of 20 when compared with the salinity of 16, and the salinity range of 16–28 was the most adequate for the cultivation of ZVI M. carcinus larvae.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Ciência e Tecnologia do Estado de PernambucoInstituto Chico Mendes de Conservação da BiodiversidadeMinistério do Meio AmbienteInstituto Agronômico de Pernambuco – IPA Departamento de Assistência Técnica e Extensão Rural – DEAT, Av. General San Martin, 1371, BongiLaboratório de Produção de Camarão – LPC Centro de Ciências Agrárias/Universidade Estadual do Maranhão – UEMA Cidade Universitária Campus Paulo VI, s/n, Jardim São CristovãoPrograma de Pós-Graduação em Recursos Pesqueiros e Aquicultura Universidade Federal Rural de Pernambuco – UFRPECentro de Aquicultura da Universidade Estadual Paulista – UNESPLaboratório de Modelagem Estatística Aplicada Departamento de Pesca e Aquicultura UFRPE, Av. Dom Manoel de Medeiros, s/n, Dois IrmãosLaboratório de Sistemas de Produção Aquícola Departamento de Pesca e Aquicultura UFRPE, Av. Dom Manoel de Medeiros, s/n, Dois IrmãosCentro de Aquicultura da Universidade Estadual Paulista – UNESPInstituto Agronômico de Pernambuco – IPAUniversidade Estadual de Maringá (UEM)Universidade Federal Rural de Pernambuco – UFRPEUniversidade Estadual Paulista (UNESP)UFRPELima, João Paulo V.Melo, Fabiana P.Ferreira, Maria Gabriela P.Flickinger, Dallas L. [UNESP]Andrade, Humber A.Correia, Eudes S.2022-04-29T08:33:20Z2022-04-29T08:33:20Z2021-11-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.ecss.2021.107602Estuarine, Coastal and Shelf Science, v. 262.0272-7714http://hdl.handle.net/11449/22959110.1016/j.ecss.2021.1076022-s2.0-85115788719Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEstuarine, Coastal and Shelf Scienceinfo:eu-repo/semantics/openAccess2024-04-09T15:43:16Zoai:repositorio.unesp.br:11449/229591Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:02:31.430643Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Lethal salinity and survival for Macrobrachium carcinus larvae subjected to osmotic stress |
title |
Lethal salinity and survival for Macrobrachium carcinus larvae subjected to osmotic stress |
spellingShingle |
Lethal salinity and survival for Macrobrachium carcinus larvae subjected to osmotic stress Lima, João Paulo V. Aquaculture Conservation Freshwater prawns Larvae Lethal salinity Logistic model |
title_short |
Lethal salinity and survival for Macrobrachium carcinus larvae subjected to osmotic stress |
title_full |
Lethal salinity and survival for Macrobrachium carcinus larvae subjected to osmotic stress |
title_fullStr |
Lethal salinity and survival for Macrobrachium carcinus larvae subjected to osmotic stress |
title_full_unstemmed |
Lethal salinity and survival for Macrobrachium carcinus larvae subjected to osmotic stress |
title_sort |
Lethal salinity and survival for Macrobrachium carcinus larvae subjected to osmotic stress |
author |
Lima, João Paulo V. |
author_facet |
Lima, João Paulo V. Melo, Fabiana P. Ferreira, Maria Gabriela P. Flickinger, Dallas L. [UNESP] Andrade, Humber A. Correia, Eudes S. |
author_role |
author |
author2 |
Melo, Fabiana P. Ferreira, Maria Gabriela P. Flickinger, Dallas L. [UNESP] Andrade, Humber A. Correia, Eudes S. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Instituto Agronômico de Pernambuco – IPA Universidade Estadual de Maringá (UEM) Universidade Federal Rural de Pernambuco – UFRPE Universidade Estadual Paulista (UNESP) UFRPE |
dc.contributor.author.fl_str_mv |
Lima, João Paulo V. Melo, Fabiana P. Ferreira, Maria Gabriela P. Flickinger, Dallas L. [UNESP] Andrade, Humber A. Correia, Eudes S. |
dc.subject.por.fl_str_mv |
Aquaculture Conservation Freshwater prawns Larvae Lethal salinity Logistic model |
topic |
Aquaculture Conservation Freshwater prawns Larvae Lethal salinity Logistic model |
description |
The present study aimed to establish an adequate salinity range for the larviculture of the Macrobrachium carcinus by determining the lethal salinity (LS50) and survival of the larvae subjected to osmotic stress tests. Newly hatched larvae were acclimated at the salinities of 16 and 20 and reared for 20–22 days until reaching Zoea stage VI (ZVI). The larvae were then subjected to two osmotic stress tests carried out in 2-L plastic recipients (1–1.6L used volume) with a density of 50 larvae/L and with constant aeration. Each salinity had three replicates. Survival was recorded every 24 h after exposure to the salinities. In Test 1, ZVI larvae were transferred without acclimatization from salinity 16 to 0, 4, 8, 12, 16 (control), 20, 24 and 28 for 96 h. In Test 2, ZVI larvae were transferred without acclimatization from salinity 20 to 0, 4, 8, 12, 16, 20 (control), 24, 28, 32, 36 and 40 for 120 h. In both tests, 100% mortality of ZVI M. carcinus larvae was observed during the first hours upon altering the salinity to 0 and 4. Total mortality occurred at 72 h in the salinity of 40. In Test 1 (96 h), the best survival was shown with salinities between 16 and 24, with a maximum of 46.7% in 20 and 24. In Test 2 (120 h), the best survival was observed in salinities between 16 and 32, with a maximum of 71.3% in 28. The linear model generated from both tests (R2 = 92.8%) indicated that survival is optimized by using a salinity between 16 and 28. The LS50 (120 h) was estimated at 11.58 in the lower range and 33.70 in the upper range. In general, M. carcinus larvae in the Pre-culture phase (Zoea stages I to VI) of the larviculture showed more resistance to osmotic stress when reared in brackishwater with a salinity of 20 when compared with the salinity of 16, and the salinity range of 16–28 was the most adequate for the cultivation of ZVI M. carcinus larvae. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-05 2022-04-29T08:33:20Z 2022-04-29T08:33:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ecss.2021.107602 Estuarine, Coastal and Shelf Science, v. 262. 0272-7714 http://hdl.handle.net/11449/229591 10.1016/j.ecss.2021.107602 2-s2.0-85115788719 |
url |
http://dx.doi.org/10.1016/j.ecss.2021.107602 http://hdl.handle.net/11449/229591 |
identifier_str_mv |
Estuarine, Coastal and Shelf Science, v. 262. 0272-7714 10.1016/j.ecss.2021.107602 2-s2.0-85115788719 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Estuarine, Coastal and Shelf Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129385811673088 |