Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.domaniend.2021.106653 http://hdl.handle.net/11449/222281 |
Resumo: | In cattle, 17β-estradiol (E2) stimulates prostaglandin F2α (PGF2α) synthesis, which causes luteolysis. Except for the well-established upregulation of oxytocin receptor gene (OXTR), molecular mechanisms of E2-induced PGF2α release in vivo remain unknown. We hypothesized that E2-induced PGF2α release requires de novo transcription of components of the PGF2α synthesis machinery. Beef cows (n = 52) were assigned to remain untreated (Control; n = 10), to receive 50% ethanol infusion intravenously (Placebo; n = 21), or 3 mg E2 in 50% ethanol infusion intravenously (Estradiol; n = 21) on day 15 (D15) after estrus. We collected a single endometrial biopsy per animal at the time of the treatment (0h; Control B0h group), 4 hours (4h; Placebo B4h group and Estradiol B4h group), or 7 hours (7h; Placebo B7h group and Estradiol B7h group) post-treatment. Compared to the Placebo group, the Estradiol group presented significantly greater 13,14-dihydro-15-keto-PGF2α concentrations between 4h and 7h and underwent earlier luteolysis. At 4h, the qPCR analysis showed a lower abundance of ESR1, ESR2 and aldo-keto reductase family 1 member B1 (AKR1B1) genes in the Estradiol B4h group, and a greater abundance of OXTR compared to the Placebo B4h group. Similarly, the E2 treatment significantly reduced the abundance of AKR1B1, and AKR1C4 in the Estradiol B7h group, compared to the placebo group. Overall, E2-induced PGF2α release and luteolysis involved an unexpected and transient downregulation of components of the PGF2α-synthesis cascade, except for OXTR, which was upregulated. Collectively, our data suggest that E2 connects newly-synthesized OXTR to pre-existing cellular machinery to synthesize PGF2α and cause luteal regression. |
id |
UNSP_f6974706a8cb8c237bcecef44b12ec56 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/222281 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattleEndometriumEstrogenLuteolysisProstaglandinIn cattle, 17β-estradiol (E2) stimulates prostaglandin F2α (PGF2α) synthesis, which causes luteolysis. Except for the well-established upregulation of oxytocin receptor gene (OXTR), molecular mechanisms of E2-induced PGF2α release in vivo remain unknown. We hypothesized that E2-induced PGF2α release requires de novo transcription of components of the PGF2α synthesis machinery. Beef cows (n = 52) were assigned to remain untreated (Control; n = 10), to receive 50% ethanol infusion intravenously (Placebo; n = 21), or 3 mg E2 in 50% ethanol infusion intravenously (Estradiol; n = 21) on day 15 (D15) after estrus. We collected a single endometrial biopsy per animal at the time of the treatment (0h; Control B0h group), 4 hours (4h; Placebo B4h group and Estradiol B4h group), or 7 hours (7h; Placebo B7h group and Estradiol B7h group) post-treatment. Compared to the Placebo group, the Estradiol group presented significantly greater 13,14-dihydro-15-keto-PGF2α concentrations between 4h and 7h and underwent earlier luteolysis. At 4h, the qPCR analysis showed a lower abundance of ESR1, ESR2 and aldo-keto reductase family 1 member B1 (AKR1B1) genes in the Estradiol B4h group, and a greater abundance of OXTR compared to the Placebo B4h group. Similarly, the E2 treatment significantly reduced the abundance of AKR1B1, and AKR1C4 in the Estradiol B7h group, compared to the placebo group. Overall, E2-induced PGF2α release and luteolysis involved an unexpected and transient downregulation of components of the PGF2α-synthesis cascade, except for OXTR, which was upregulated. Collectively, our data suggest that E2 connects newly-synthesized OXTR to pre-existing cellular machinery to synthesize PGF2α and cause luteal regression.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Agrarian Sciences Center State University of Maranhão Tocantine RegionDepartment of Animal Reproduction University of São PauloNorth Florida Research and Education Center Institute of Food and Agricultural Sciences University of FloridaDepartment of Animal Sciences University of FloridaDepartment of Pharmacology and Biotechnology São Paulo State UniversityDepartment of Animal Science Luiz de Queiroz College of Agriculture University of São PauloDepartment of Animal Sciences São Paulo State UniversityDepartment of Pharmacology and Biotechnology São Paulo State UniversityDepartment of Animal Sciences São Paulo State UniversityFAPESP: 2015/03331-3)State University of Maranhão Tocantine RegionUniversidade de São Paulo (USP)University of FloridaUniversidade Estadual Paulista (UNESP)Oliveira, M. L.Mello, B. P.Gonella-Diaza, A. M.Scolari, S. C.Pugliesi, G.Martins, T.Feltrin, I. R. [UNESP]Sartori, R.Canavessi, A. M.O.Binelli, M.Membrive, C. M.B. [UNESP]2022-04-28T19:43:42Z2022-04-28T19:43:42Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.domaniend.2021.106653Domestic Animal Endocrinology, v. 78.0739-7240http://hdl.handle.net/11449/22228110.1016/j.domaniend.2021.1066532-s2.0-85113497255Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDomestic Animal Endocrinologyinfo:eu-repo/semantics/openAccess2022-04-28T19:43:42Zoai:repositorio.unesp.br:11449/222281Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:26:35.086205Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle |
title |
Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle |
spellingShingle |
Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle Oliveira, M. L. Endometrium Estrogen Luteolysis Prostaglandin |
title_short |
Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle |
title_full |
Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle |
title_fullStr |
Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle |
title_full_unstemmed |
Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle |
title_sort |
Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle |
author |
Oliveira, M. L. |
author_facet |
Oliveira, M. L. Mello, B. P. Gonella-Diaza, A. M. Scolari, S. C. Pugliesi, G. Martins, T. Feltrin, I. R. [UNESP] Sartori, R. Canavessi, A. M.O. Binelli, M. Membrive, C. M.B. [UNESP] |
author_role |
author |
author2 |
Mello, B. P. Gonella-Diaza, A. M. Scolari, S. C. Pugliesi, G. Martins, T. Feltrin, I. R. [UNESP] Sartori, R. Canavessi, A. M.O. Binelli, M. Membrive, C. M.B. [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
State University of Maranhão Tocantine Region Universidade de São Paulo (USP) University of Florida Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Oliveira, M. L. Mello, B. P. Gonella-Diaza, A. M. Scolari, S. C. Pugliesi, G. Martins, T. Feltrin, I. R. [UNESP] Sartori, R. Canavessi, A. M.O. Binelli, M. Membrive, C. M.B. [UNESP] |
dc.subject.por.fl_str_mv |
Endometrium Estrogen Luteolysis Prostaglandin |
topic |
Endometrium Estrogen Luteolysis Prostaglandin |
description |
In cattle, 17β-estradiol (E2) stimulates prostaglandin F2α (PGF2α) synthesis, which causes luteolysis. Except for the well-established upregulation of oxytocin receptor gene (OXTR), molecular mechanisms of E2-induced PGF2α release in vivo remain unknown. We hypothesized that E2-induced PGF2α release requires de novo transcription of components of the PGF2α synthesis machinery. Beef cows (n = 52) were assigned to remain untreated (Control; n = 10), to receive 50% ethanol infusion intravenously (Placebo; n = 21), or 3 mg E2 in 50% ethanol infusion intravenously (Estradiol; n = 21) on day 15 (D15) after estrus. We collected a single endometrial biopsy per animal at the time of the treatment (0h; Control B0h group), 4 hours (4h; Placebo B4h group and Estradiol B4h group), or 7 hours (7h; Placebo B7h group and Estradiol B7h group) post-treatment. Compared to the Placebo group, the Estradiol group presented significantly greater 13,14-dihydro-15-keto-PGF2α concentrations between 4h and 7h and underwent earlier luteolysis. At 4h, the qPCR analysis showed a lower abundance of ESR1, ESR2 and aldo-keto reductase family 1 member B1 (AKR1B1) genes in the Estradiol B4h group, and a greater abundance of OXTR compared to the Placebo B4h group. Similarly, the E2 treatment significantly reduced the abundance of AKR1B1, and AKR1C4 in the Estradiol B7h group, compared to the placebo group. Overall, E2-induced PGF2α release and luteolysis involved an unexpected and transient downregulation of components of the PGF2α-synthesis cascade, except for OXTR, which was upregulated. Collectively, our data suggest that E2 connects newly-synthesized OXTR to pre-existing cellular machinery to synthesize PGF2α and cause luteal regression. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:43:42Z 2022-04-28T19:43:42Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.domaniend.2021.106653 Domestic Animal Endocrinology, v. 78. 0739-7240 http://hdl.handle.net/11449/222281 10.1016/j.domaniend.2021.106653 2-s2.0-85113497255 |
url |
http://dx.doi.org/10.1016/j.domaniend.2021.106653 http://hdl.handle.net/11449/222281 |
identifier_str_mv |
Domestic Animal Endocrinology, v. 78. 0739-7240 10.1016/j.domaniend.2021.106653 2-s2.0-85113497255 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Domestic Animal Endocrinology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128652966100992 |