Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle

Detalhes bibliográficos
Autor(a) principal: Oliveira, M. L.
Data de Publicação: 2022
Outros Autores: Mello, B. P., Gonella-Diaza, A. M., Scolari, S. C., Pugliesi, G., Martins, T., Feltrin, I. R. [UNESP], Sartori, R., Canavessi, A. M.O., Binelli, M., Membrive, C. M.B. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.domaniend.2021.106653
http://hdl.handle.net/11449/222281
Resumo: In cattle, 17β-estradiol (E2) stimulates prostaglandin F2α (PGF2α) synthesis, which causes luteolysis. Except for the well-established upregulation of oxytocin receptor gene (OXTR), molecular mechanisms of E2-induced PGF2α release in vivo remain unknown. We hypothesized that E2-induced PGF2α release requires de novo transcription of components of the PGF2α synthesis machinery. Beef cows (n = 52) were assigned to remain untreated (Control; n = 10), to receive 50% ethanol infusion intravenously (Placebo; n = 21), or 3 mg E2 in 50% ethanol infusion intravenously (Estradiol; n = 21) on day 15 (D15) after estrus. We collected a single endometrial biopsy per animal at the time of the treatment (0h; Control B0h group), 4 hours (4h; Placebo B4h group and Estradiol B4h group), or 7 hours (7h; Placebo B7h group and Estradiol B7h group) post-treatment. Compared to the Placebo group, the Estradiol group presented significantly greater 13,14-dihydro-15-keto-PGF2α concentrations between 4h and 7h and underwent earlier luteolysis. At 4h, the qPCR analysis showed a lower abundance of ESR1, ESR2 and aldo-keto reductase family 1 member B1 (AKR1B1) genes in the Estradiol B4h group, and a greater abundance of OXTR compared to the Placebo B4h group. Similarly, the E2 treatment significantly reduced the abundance of AKR1B1, and AKR1C4 in the Estradiol B7h group, compared to the placebo group. Overall, E2-induced PGF2α release and luteolysis involved an unexpected and transient downregulation of components of the PGF2α-synthesis cascade, except for OXTR, which was upregulated. Collectively, our data suggest that E2 connects newly-synthesized OXTR to pre-existing cellular machinery to synthesize PGF2α and cause luteal regression.
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spelling Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattleEndometriumEstrogenLuteolysisProstaglandinIn cattle, 17β-estradiol (E2) stimulates prostaglandin F2α (PGF2α) synthesis, which causes luteolysis. Except for the well-established upregulation of oxytocin receptor gene (OXTR), molecular mechanisms of E2-induced PGF2α release in vivo remain unknown. We hypothesized that E2-induced PGF2α release requires de novo transcription of components of the PGF2α synthesis machinery. Beef cows (n = 52) were assigned to remain untreated (Control; n = 10), to receive 50% ethanol infusion intravenously (Placebo; n = 21), or 3 mg E2 in 50% ethanol infusion intravenously (Estradiol; n = 21) on day 15 (D15) after estrus. We collected a single endometrial biopsy per animal at the time of the treatment (0h; Control B0h group), 4 hours (4h; Placebo B4h group and Estradiol B4h group), or 7 hours (7h; Placebo B7h group and Estradiol B7h group) post-treatment. Compared to the Placebo group, the Estradiol group presented significantly greater 13,14-dihydro-15-keto-PGF2α concentrations between 4h and 7h and underwent earlier luteolysis. At 4h, the qPCR analysis showed a lower abundance of ESR1, ESR2 and aldo-keto reductase family 1 member B1 (AKR1B1) genes in the Estradiol B4h group, and a greater abundance of OXTR compared to the Placebo B4h group. Similarly, the E2 treatment significantly reduced the abundance of AKR1B1, and AKR1C4 in the Estradiol B7h group, compared to the placebo group. Overall, E2-induced PGF2α release and luteolysis involved an unexpected and transient downregulation of components of the PGF2α-synthesis cascade, except for OXTR, which was upregulated. Collectively, our data suggest that E2 connects newly-synthesized OXTR to pre-existing cellular machinery to synthesize PGF2α and cause luteal regression.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Agrarian Sciences Center State University of Maranhão Tocantine RegionDepartment of Animal Reproduction University of São PauloNorth Florida Research and Education Center Institute of Food and Agricultural Sciences University of FloridaDepartment of Animal Sciences University of FloridaDepartment of Pharmacology and Biotechnology São Paulo State UniversityDepartment of Animal Science Luiz de Queiroz College of Agriculture University of São PauloDepartment of Animal Sciences São Paulo State UniversityDepartment of Pharmacology and Biotechnology São Paulo State UniversityDepartment of Animal Sciences São Paulo State UniversityFAPESP: 2015/03331-3)State University of Maranhão Tocantine RegionUniversidade de São Paulo (USP)University of FloridaUniversidade Estadual Paulista (UNESP)Oliveira, M. L.Mello, B. P.Gonella-Diaza, A. M.Scolari, S. C.Pugliesi, G.Martins, T.Feltrin, I. R. [UNESP]Sartori, R.Canavessi, A. M.O.Binelli, M.Membrive, C. M.B. [UNESP]2022-04-28T19:43:42Z2022-04-28T19:43:42Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.domaniend.2021.106653Domestic Animal Endocrinology, v. 78.0739-7240http://hdl.handle.net/11449/22228110.1016/j.domaniend.2021.1066532-s2.0-85113497255Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDomestic Animal Endocrinologyinfo:eu-repo/semantics/openAccess2022-04-28T19:43:42Zoai:repositorio.unesp.br:11449/222281Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:26:35.086205Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle
title Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle
spellingShingle Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle
Oliveira, M. L.
Endometrium
Estrogen
Luteolysis
Prostaglandin
title_short Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle
title_full Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle
title_fullStr Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle
title_full_unstemmed Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle
title_sort Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle
author Oliveira, M. L.
author_facet Oliveira, M. L.
Mello, B. P.
Gonella-Diaza, A. M.
Scolari, S. C.
Pugliesi, G.
Martins, T.
Feltrin, I. R. [UNESP]
Sartori, R.
Canavessi, A. M.O.
Binelli, M.
Membrive, C. M.B. [UNESP]
author_role author
author2 Mello, B. P.
Gonella-Diaza, A. M.
Scolari, S. C.
Pugliesi, G.
Martins, T.
Feltrin, I. R. [UNESP]
Sartori, R.
Canavessi, A. M.O.
Binelli, M.
Membrive, C. M.B. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv State University of Maranhão Tocantine Region
Universidade de São Paulo (USP)
University of Florida
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Oliveira, M. L.
Mello, B. P.
Gonella-Diaza, A. M.
Scolari, S. C.
Pugliesi, G.
Martins, T.
Feltrin, I. R. [UNESP]
Sartori, R.
Canavessi, A. M.O.
Binelli, M.
Membrive, C. M.B. [UNESP]
dc.subject.por.fl_str_mv Endometrium
Estrogen
Luteolysis
Prostaglandin
topic Endometrium
Estrogen
Luteolysis
Prostaglandin
description In cattle, 17β-estradiol (E2) stimulates prostaglandin F2α (PGF2α) synthesis, which causes luteolysis. Except for the well-established upregulation of oxytocin receptor gene (OXTR), molecular mechanisms of E2-induced PGF2α release in vivo remain unknown. We hypothesized that E2-induced PGF2α release requires de novo transcription of components of the PGF2α synthesis machinery. Beef cows (n = 52) were assigned to remain untreated (Control; n = 10), to receive 50% ethanol infusion intravenously (Placebo; n = 21), or 3 mg E2 in 50% ethanol infusion intravenously (Estradiol; n = 21) on day 15 (D15) after estrus. We collected a single endometrial biopsy per animal at the time of the treatment (0h; Control B0h group), 4 hours (4h; Placebo B4h group and Estradiol B4h group), or 7 hours (7h; Placebo B7h group and Estradiol B7h group) post-treatment. Compared to the Placebo group, the Estradiol group presented significantly greater 13,14-dihydro-15-keto-PGF2α concentrations between 4h and 7h and underwent earlier luteolysis. At 4h, the qPCR analysis showed a lower abundance of ESR1, ESR2 and aldo-keto reductase family 1 member B1 (AKR1B1) genes in the Estradiol B4h group, and a greater abundance of OXTR compared to the Placebo B4h group. Similarly, the E2 treatment significantly reduced the abundance of AKR1B1, and AKR1C4 in the Estradiol B7h group, compared to the placebo group. Overall, E2-induced PGF2α release and luteolysis involved an unexpected and transient downregulation of components of the PGF2α-synthesis cascade, except for OXTR, which was upregulated. Collectively, our data suggest that E2 connects newly-synthesized OXTR to pre-existing cellular machinery to synthesize PGF2α and cause luteal regression.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-28T19:43:42Z
2022-04-28T19:43:42Z
2022-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.domaniend.2021.106653
Domestic Animal Endocrinology, v. 78.
0739-7240
http://hdl.handle.net/11449/222281
10.1016/j.domaniend.2021.106653
2-s2.0-85113497255
url http://dx.doi.org/10.1016/j.domaniend.2021.106653
http://hdl.handle.net/11449/222281
identifier_str_mv Domestic Animal Endocrinology, v. 78.
0739-7240
10.1016/j.domaniend.2021.106653
2-s2.0-85113497255
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Domestic Animal Endocrinology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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