Effect of a probiotic beverage consumption (Enterococcus faecium CRL 183 and Bifidobacterium longum ATCC 15707) in rats with chemically induced colitis
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0175935 http://hdl.handle.net/11449/174523 |
Resumo: | Background Some probiotic strains have the potential to assist in relieving the symptoms of inflammatory bowel disease. The impact of daily ingestion of a soy-based product fermented by Enterococcus faecium CRL 183 and Lactobacillus helveticus 416 with the addition of Bifidobacterium longum ATCC 15707 on chemically induced colitis has been investigated thereof within a period of 30 days. Methods Colitis was induced by dextran sulfate sodium. The animals were randomly assigned into five groups: Group C: negative control; Group CL: positive control; Group CLF: DSS with the fermented product; Group CLP: DSS with the non-fermented product (placebo); Group CLS: DSS with sulfasalazine. The following parameters were monitored: disease activity index, fecal microbial analyses, gastrointestinal survival of probiotic microorganisms and short-chain fatty acids concentration in the feces. At the end of the protocol the animals' colons were removed so as to conduct a macroscopical and histopathological analysis, cytokines and nitrite quantification. Results Animals belonging to the CLF group showed fewer symptoms of colitis during the induction period and a lower degree of inflammation and ulceration in their colon compared to the CL, CLS and CLP groups (p<0.05). The colon of the animals in groups CL and CLS presented severe crypt damage, which was absent in CLF and CLP groups. A significant increase in the population of Lactobacillus spp. and Bifidobacterium spp. at the end of the protocol was verified only in the CLF animals (p<0.05). This group also showed an increase in short-chain fatty acids (propionate and acetate). Furthermore, the intestinal survival of E. faecium CRL 183 and B. longum ATCC 15707 in the CLF group has been confirmed by biochemical and molecular analyzes. Conclusions The obtained results suggest that a regular intake of the probiotic product, and placebo to a lesser extent, can reduce the severity of DSS-induced colitis on rats. |
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Effect of a probiotic beverage consumption (Enterococcus faecium CRL 183 and Bifidobacterium longum ATCC 15707) in rats with chemically induced colitisBackground Some probiotic strains have the potential to assist in relieving the symptoms of inflammatory bowel disease. The impact of daily ingestion of a soy-based product fermented by Enterococcus faecium CRL 183 and Lactobacillus helveticus 416 with the addition of Bifidobacterium longum ATCC 15707 on chemically induced colitis has been investigated thereof within a period of 30 days. Methods Colitis was induced by dextran sulfate sodium. The animals were randomly assigned into five groups: Group C: negative control; Group CL: positive control; Group CLF: DSS with the fermented product; Group CLP: DSS with the non-fermented product (placebo); Group CLS: DSS with sulfasalazine. The following parameters were monitored: disease activity index, fecal microbial analyses, gastrointestinal survival of probiotic microorganisms and short-chain fatty acids concentration in the feces. At the end of the protocol the animals' colons were removed so as to conduct a macroscopical and histopathological analysis, cytokines and nitrite quantification. Results Animals belonging to the CLF group showed fewer symptoms of colitis during the induction period and a lower degree of inflammation and ulceration in their colon compared to the CL, CLS and CLP groups (p<0.05). The colon of the animals in groups CL and CLS presented severe crypt damage, which was absent in CLF and CLP groups. A significant increase in the population of Lactobacillus spp. and Bifidobacterium spp. at the end of the protocol was verified only in the CLF animals (p<0.05). This group also showed an increase in short-chain fatty acids (propionate and acetate). Furthermore, the intestinal survival of E. faecium CRL 183 and B. longum ATCC 15707 in the CLF group has been confirmed by biochemical and molecular analyzes. Conclusions The obtained results suggest that a regular intake of the probiotic product, and placebo to a lesser extent, can reduce the severity of DSS-induced colitis on rats.Universidade Estadual Paulista (UNESP) Faculdade de Ciências Farmacêuticas Departamento de Alimentos e NutricËãoDepartamento de Tecnologia Bioquímico-Farmacêutica Faculdade de Ciências Farmacêuticas Universidade de São PauloUniversidade de São Paulo (USP) Faculdade de Medicina de Ribeirão Preto Departamento de Bioquimica e ImunologiaUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Farmacêuticas Departamento de Ciências BioloÂgicasUniversidade Estadual Paulista (UNESP) Faculdade de Odontologia Departametno de Fisiologia e PatologiaUniversidade de São Paulo (USP) Faculdade de Engenharia Departamento de Hidraúlica e SaneamentoCentro de Referencia Para Lactobacilos CERELAUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Farmacêuticas Departamento de Alimentos e NutricËãoUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Farmacêuticas Departamento de Ciências BioloÂgicasUniversidade Estadual Paulista (UNESP) Faculdade de Odontologia Departametno de Fisiologia e PatologiaUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)CERELACeliberto, Larissa Sbaglia [UNESP]Bedani, RaquelDejani, Naiara Naiana [UNESP]De Medeiros, Alexandra Ivo [UNESP]Zuanon, José Antonio Sampaio [UNESP]Spolidorio, Luis Carlos [UNESP]Adorno, Maria Angela TallaricoVaresche, Maria Bernadete AmâncioGalvão, Fábio Carrilho [UNESP]Valentini, Sandro Roberto [UNESP]De Valdez, Graciela FontRossi, Elizeu Antonio [UNESP]Cavallini, Daniela Cardoso Umbelino [UNESP]2018-12-11T17:11:33Z2018-12-11T17:11:33Z2017-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0175935PLoS ONE, v. 12, n. 4, 2017.1932-6203http://hdl.handle.net/11449/17452310.1371/journal.pone.01759352-s2.0-850185773632-s2.0-85018577363.pdf2640929291808415Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONE1,164info:eu-repo/semantics/openAccess2024-06-24T13:07:39Zoai:repositorio.unesp.br:11449/174523Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:32:35.448184Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of a probiotic beverage consumption (Enterococcus faecium CRL 183 and Bifidobacterium longum ATCC 15707) in rats with chemically induced colitis |
title |
Effect of a probiotic beverage consumption (Enterococcus faecium CRL 183 and Bifidobacterium longum ATCC 15707) in rats with chemically induced colitis |
spellingShingle |
Effect of a probiotic beverage consumption (Enterococcus faecium CRL 183 and Bifidobacterium longum ATCC 15707) in rats with chemically induced colitis Celiberto, Larissa Sbaglia [UNESP] |
title_short |
Effect of a probiotic beverage consumption (Enterococcus faecium CRL 183 and Bifidobacterium longum ATCC 15707) in rats with chemically induced colitis |
title_full |
Effect of a probiotic beverage consumption (Enterococcus faecium CRL 183 and Bifidobacterium longum ATCC 15707) in rats with chemically induced colitis |
title_fullStr |
Effect of a probiotic beverage consumption (Enterococcus faecium CRL 183 and Bifidobacterium longum ATCC 15707) in rats with chemically induced colitis |
title_full_unstemmed |
Effect of a probiotic beverage consumption (Enterococcus faecium CRL 183 and Bifidobacterium longum ATCC 15707) in rats with chemically induced colitis |
title_sort |
Effect of a probiotic beverage consumption (Enterococcus faecium CRL 183 and Bifidobacterium longum ATCC 15707) in rats with chemically induced colitis |
author |
Celiberto, Larissa Sbaglia [UNESP] |
author_facet |
Celiberto, Larissa Sbaglia [UNESP] Bedani, Raquel Dejani, Naiara Naiana [UNESP] De Medeiros, Alexandra Ivo [UNESP] Zuanon, José Antonio Sampaio [UNESP] Spolidorio, Luis Carlos [UNESP] Adorno, Maria Angela Tallarico Varesche, Maria Bernadete Amâncio Galvão, Fábio Carrilho [UNESP] Valentini, Sandro Roberto [UNESP] De Valdez, Graciela Font Rossi, Elizeu Antonio [UNESP] Cavallini, Daniela Cardoso Umbelino [UNESP] |
author_role |
author |
author2 |
Bedani, Raquel Dejani, Naiara Naiana [UNESP] De Medeiros, Alexandra Ivo [UNESP] Zuanon, José Antonio Sampaio [UNESP] Spolidorio, Luis Carlos [UNESP] Adorno, Maria Angela Tallarico Varesche, Maria Bernadete Amâncio Galvão, Fábio Carrilho [UNESP] Valentini, Sandro Roberto [UNESP] De Valdez, Graciela Font Rossi, Elizeu Antonio [UNESP] Cavallini, Daniela Cardoso Umbelino [UNESP] |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) CERELA |
dc.contributor.author.fl_str_mv |
Celiberto, Larissa Sbaglia [UNESP] Bedani, Raquel Dejani, Naiara Naiana [UNESP] De Medeiros, Alexandra Ivo [UNESP] Zuanon, José Antonio Sampaio [UNESP] Spolidorio, Luis Carlos [UNESP] Adorno, Maria Angela Tallarico Varesche, Maria Bernadete Amâncio Galvão, Fábio Carrilho [UNESP] Valentini, Sandro Roberto [UNESP] De Valdez, Graciela Font Rossi, Elizeu Antonio [UNESP] Cavallini, Daniela Cardoso Umbelino [UNESP] |
description |
Background Some probiotic strains have the potential to assist in relieving the symptoms of inflammatory bowel disease. The impact of daily ingestion of a soy-based product fermented by Enterococcus faecium CRL 183 and Lactobacillus helveticus 416 with the addition of Bifidobacterium longum ATCC 15707 on chemically induced colitis has been investigated thereof within a period of 30 days. Methods Colitis was induced by dextran sulfate sodium. The animals were randomly assigned into five groups: Group C: negative control; Group CL: positive control; Group CLF: DSS with the fermented product; Group CLP: DSS with the non-fermented product (placebo); Group CLS: DSS with sulfasalazine. The following parameters were monitored: disease activity index, fecal microbial analyses, gastrointestinal survival of probiotic microorganisms and short-chain fatty acids concentration in the feces. At the end of the protocol the animals' colons were removed so as to conduct a macroscopical and histopathological analysis, cytokines and nitrite quantification. Results Animals belonging to the CLF group showed fewer symptoms of colitis during the induction period and a lower degree of inflammation and ulceration in their colon compared to the CL, CLS and CLP groups (p<0.05). The colon of the animals in groups CL and CLS presented severe crypt damage, which was absent in CLF and CLP groups. A significant increase in the population of Lactobacillus spp. and Bifidobacterium spp. at the end of the protocol was verified only in the CLF animals (p<0.05). This group also showed an increase in short-chain fatty acids (propionate and acetate). Furthermore, the intestinal survival of E. faecium CRL 183 and B. longum ATCC 15707 in the CLF group has been confirmed by biochemical and molecular analyzes. Conclusions The obtained results suggest that a regular intake of the probiotic product, and placebo to a lesser extent, can reduce the severity of DSS-induced colitis on rats. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04-01 2018-12-11T17:11:33Z 2018-12-11T17:11:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0175935 PLoS ONE, v. 12, n. 4, 2017. 1932-6203 http://hdl.handle.net/11449/174523 10.1371/journal.pone.0175935 2-s2.0-85018577363 2-s2.0-85018577363.pdf 2640929291808415 |
url |
http://dx.doi.org/10.1371/journal.pone.0175935 http://hdl.handle.net/11449/174523 |
identifier_str_mv |
PLoS ONE, v. 12, n. 4, 2017. 1932-6203 10.1371/journal.pone.0175935 2-s2.0-85018577363 2-s2.0-85018577363.pdf 2640929291808415 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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PLoS ONE 1,164 |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
application/pdf |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128944503783424 |