Identification of Aortic Proteins Involved in Arterial Stiffness in Spontaneously Hypertensive Rats Treated With Perindopril:A Proteomic Approach

Detalhes bibliográficos
Autor(a) principal: Miotto, Danyelle S. [UNESP]
Data de Publicação: 2021
Outros Autores: Dionizio, Aline, Jacomini, André M. [UNESP], Zago, Anderson S. [UNESP], Buzalaf, Marília Afonso Rabelo, Amaral, Sandra L. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fphys.2021.624515
http://hdl.handle.net/11449/207913
Resumo: Arterial stiffness, frequently associated with hypertension, is associated with disorganization of the vascular wall and has been recognized as an independent predictor of all-cause mortality. The identification of the molecular mechanisms involved in aortic stiffness would be an emerging target for hypertension therapeutic intervention. This study evaluated the effects of perindopril on pulse wave velocity (PWV) and on the differentially expressed proteins in aorta of spontaneously hypertensive rats (SHR), using a proteomic approach. SHR and Wistar rats were treated with perindopril (SHRP) or water (SHRc and Wistar rats) for 8 weeks. At the end, SHRC presented higher systolic blood pressure (SBP, +70%) and PWV (+31%) compared with Wistar rats. SHRP had higher values of nitrite concentration and lower PWV compared with SHRC. From 21 upregulated proteins in the aortic wall from SHRC, most of them were involved with the actin cytoskeleton organization, like Tropomyosin and Cofilin-1. After perindopril treatment, there was an upregulation of the GDP dissociation inhibitors (GDIs), which normally inhibits the RhoA/Rho-kinase/cofilin-1 pathway and may contribute to decreased arterial stiffening. In conclusion, the results of the present study revealed that treatment with perindopril reduced SBP and PWV in SHR. In addition, the proteomic analysis in aorta suggested, for the first time, that the RhoA/Rho-kinase/Cofilin-1 pathway may be inhibited by perindopril-induced upregulation of GDIs or increases in NO bioavailability in SHR. Therefore, we may propose that activation of GDIs or inhibition of RhoA/Rho-kinase pathway could be a possible strategy to treat arterial stiffness.
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spelling Identification of Aortic Proteins Involved in Arterial Stiffness in Spontaneously Hypertensive Rats Treated With Perindopril:A Proteomic ApproachACE inhibitoraorta arteryhypertensionproteomic analysispulse wave velocityArterial stiffness, frequently associated with hypertension, is associated with disorganization of the vascular wall and has been recognized as an independent predictor of all-cause mortality. The identification of the molecular mechanisms involved in aortic stiffness would be an emerging target for hypertension therapeutic intervention. This study evaluated the effects of perindopril on pulse wave velocity (PWV) and on the differentially expressed proteins in aorta of spontaneously hypertensive rats (SHR), using a proteomic approach. SHR and Wistar rats were treated with perindopril (SHRP) or water (SHRc and Wistar rats) for 8 weeks. At the end, SHRC presented higher systolic blood pressure (SBP, +70%) and PWV (+31%) compared with Wistar rats. SHRP had higher values of nitrite concentration and lower PWV compared with SHRC. From 21 upregulated proteins in the aortic wall from SHRC, most of them were involved with the actin cytoskeleton organization, like Tropomyosin and Cofilin-1. After perindopril treatment, there was an upregulation of the GDP dissociation inhibitors (GDIs), which normally inhibits the RhoA/Rho-kinase/cofilin-1 pathway and may contribute to decreased arterial stiffening. In conclusion, the results of the present study revealed that treatment with perindopril reduced SBP and PWV in SHR. In addition, the proteomic analysis in aorta suggested, for the first time, that the RhoA/Rho-kinase/Cofilin-1 pathway may be inhibited by perindopril-induced upregulation of GDIs or increases in NO bioavailability in SHR. Therefore, we may propose that activation of GDIs or inhibition of RhoA/Rho-kinase pathway could be a possible strategy to treat arterial stiffness.Joint Graduate Program in Physiological Sciences Federal University of Sao Carlos and São Paulo State University UFSCar/UNESPDepartment of Biological Sciences Bauru School of Dentistry University of São PauloPost-Graduate Program in Movement Sciences São Paulo State UniversityDepartment of Physical Education School of Sciences São Paulo State UniversityJoint Graduate Program in Physiological Sciences Federal University of Sao Carlos and São Paulo State University UFSCar/UNESPPost-Graduate Program in Movement Sciences São Paulo State UniversityDepartment of Physical Education School of Sciences São Paulo State UniversityUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Miotto, Danyelle S. [UNESP]Dionizio, AlineJacomini, André M. [UNESP]Zago, Anderson S. [UNESP]Buzalaf, Marília Afonso RabeloAmaral, Sandra L. [UNESP]2021-06-25T11:03:07Z2021-06-25T11:03:07Z2021-02-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fphys.2021.624515Frontiers in Physiology, v. 12.1664-042Xhttp://hdl.handle.net/11449/20791310.3389/fphys.2021.6245152-s2.0-85101585223Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Physiologyinfo:eu-repo/semantics/openAccess2024-04-23T15:23:49Zoai:repositorio.unesp.br:11449/207913Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:02:56.464273Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Identification of Aortic Proteins Involved in Arterial Stiffness in Spontaneously Hypertensive Rats Treated With Perindopril:A Proteomic Approach
title Identification of Aortic Proteins Involved in Arterial Stiffness in Spontaneously Hypertensive Rats Treated With Perindopril:A Proteomic Approach
spellingShingle Identification of Aortic Proteins Involved in Arterial Stiffness in Spontaneously Hypertensive Rats Treated With Perindopril:A Proteomic Approach
Miotto, Danyelle S. [UNESP]
ACE inhibitor
aorta artery
hypertension
proteomic analysis
pulse wave velocity
title_short Identification of Aortic Proteins Involved in Arterial Stiffness in Spontaneously Hypertensive Rats Treated With Perindopril:A Proteomic Approach
title_full Identification of Aortic Proteins Involved in Arterial Stiffness in Spontaneously Hypertensive Rats Treated With Perindopril:A Proteomic Approach
title_fullStr Identification of Aortic Proteins Involved in Arterial Stiffness in Spontaneously Hypertensive Rats Treated With Perindopril:A Proteomic Approach
title_full_unstemmed Identification of Aortic Proteins Involved in Arterial Stiffness in Spontaneously Hypertensive Rats Treated With Perindopril:A Proteomic Approach
title_sort Identification of Aortic Proteins Involved in Arterial Stiffness in Spontaneously Hypertensive Rats Treated With Perindopril:A Proteomic Approach
author Miotto, Danyelle S. [UNESP]
author_facet Miotto, Danyelle S. [UNESP]
Dionizio, Aline
Jacomini, André M. [UNESP]
Zago, Anderson S. [UNESP]
Buzalaf, Marília Afonso Rabelo
Amaral, Sandra L. [UNESP]
author_role author
author2 Dionizio, Aline
Jacomini, André M. [UNESP]
Zago, Anderson S. [UNESP]
Buzalaf, Marília Afonso Rabelo
Amaral, Sandra L. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Miotto, Danyelle S. [UNESP]
Dionizio, Aline
Jacomini, André M. [UNESP]
Zago, Anderson S. [UNESP]
Buzalaf, Marília Afonso Rabelo
Amaral, Sandra L. [UNESP]
dc.subject.por.fl_str_mv ACE inhibitor
aorta artery
hypertension
proteomic analysis
pulse wave velocity
topic ACE inhibitor
aorta artery
hypertension
proteomic analysis
pulse wave velocity
description Arterial stiffness, frequently associated with hypertension, is associated with disorganization of the vascular wall and has been recognized as an independent predictor of all-cause mortality. The identification of the molecular mechanisms involved in aortic stiffness would be an emerging target for hypertension therapeutic intervention. This study evaluated the effects of perindopril on pulse wave velocity (PWV) and on the differentially expressed proteins in aorta of spontaneously hypertensive rats (SHR), using a proteomic approach. SHR and Wistar rats were treated with perindopril (SHRP) or water (SHRc and Wistar rats) for 8 weeks. At the end, SHRC presented higher systolic blood pressure (SBP, +70%) and PWV (+31%) compared with Wistar rats. SHRP had higher values of nitrite concentration and lower PWV compared with SHRC. From 21 upregulated proteins in the aortic wall from SHRC, most of them were involved with the actin cytoskeleton organization, like Tropomyosin and Cofilin-1. After perindopril treatment, there was an upregulation of the GDP dissociation inhibitors (GDIs), which normally inhibits the RhoA/Rho-kinase/cofilin-1 pathway and may contribute to decreased arterial stiffening. In conclusion, the results of the present study revealed that treatment with perindopril reduced SBP and PWV in SHR. In addition, the proteomic analysis in aorta suggested, for the first time, that the RhoA/Rho-kinase/Cofilin-1 pathway may be inhibited by perindopril-induced upregulation of GDIs or increases in NO bioavailability in SHR. Therefore, we may propose that activation of GDIs or inhibition of RhoA/Rho-kinase pathway could be a possible strategy to treat arterial stiffness.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T11:03:07Z
2021-06-25T11:03:07Z
2021-02-10
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fphys.2021.624515
Frontiers in Physiology, v. 12.
1664-042X
http://hdl.handle.net/11449/207913
10.3389/fphys.2021.624515
2-s2.0-85101585223
url http://dx.doi.org/10.3389/fphys.2021.624515
http://hdl.handle.net/11449/207913
identifier_str_mv Frontiers in Physiology, v. 12.
1664-042X
10.3389/fphys.2021.624515
2-s2.0-85101585223
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Physiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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