Development and cross-validation of microsatellite markers for Rauvolfia weddeliana Mull.Arg. (Apocynaceae) species complex
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s40415-018-0465-9 http://hdl.handle.net/11449/164548 |
Resumo: | Rauvolfia weddeliana is an endemic species restricted to plateau landscapes in South American savannahs. Rapid loss of habitat and expansion of agriculture in Central-West Brazil have critically reduced the original extent of savannahs, representing a major threat to its biotic diversity. Due to the discontinuous distribution of R. weddeliana and the vulnerability of its habitats, it is crucial to estimate the genetic diversity of remaining populations. The application of microsatellite markers is a useful approach with relative low cost and high informative potential for studies related to conservation genetics and population genetics. The development of specific libraries for endangered species may aid future studies about the connectivity of populations, reproductive biology, and genetic diversity. We developed microsatellite markers for R. weddeliana and tested the transferability of the markers to a closely related species, R. gracilis. Ten microsatellite markers were identified, and a set of primers for their amplification is presented. Most identified motifs were dimers, with lengths from 18 to 74 base pairs. Nine markers presented high informative potential (PIC > 0.5). The set of markers developed in this study will support further investigations in population genetics of R. weddeliana and possibility of closely related species. |
id |
UNSP_f8285412dbd0319058b8d608991cfb8e |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/164548 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Development and cross-validation of microsatellite markers for Rauvolfia weddeliana Mull.Arg. (Apocynaceae) species complexCerradoConservation geneticsPopulation geneticsSSRRauvolfia weddeliana is an endemic species restricted to plateau landscapes in South American savannahs. Rapid loss of habitat and expansion of agriculture in Central-West Brazil have critically reduced the original extent of savannahs, representing a major threat to its biotic diversity. Due to the discontinuous distribution of R. weddeliana and the vulnerability of its habitats, it is crucial to estimate the genetic diversity of remaining populations. The application of microsatellite markers is a useful approach with relative low cost and high informative potential for studies related to conservation genetics and population genetics. The development of specific libraries for endangered species may aid future studies about the connectivity of populations, reproductive biology, and genetic diversity. We developed microsatellite markers for R. weddeliana and tested the transferability of the markers to a closely related species, R. gracilis. Ten microsatellite markers were identified, and a set of primers for their amplification is presented. Most identified motifs were dimers, with lengths from 18 to 74 base pairs. Nine markers presented high informative potential (PIC > 0.5). The set of markers developed in this study will support further investigations in population genetics of R. weddeliana and possibility of closely related species.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundo de Apoio ao Ensino, a Pesquisa e Extensao-FAEPEXPrograma de Apoio a Pos-Graduacao-PROAP, from Universidade Estadual Paulista Julio de Mesquita FilhoUniv Estadual Paulista, Dept Bot, Inst Biociencias Botucatu, Rua Prof Dr Antonio Celso Wagner Zanin S-N, BR-18618689 Botucatu, SP, BrazilUniv Estadual Campinas, Dept Biol Vegetal, Rua Monteiro Lobato 255 Cidade Univ Zeferino Vaz, BR-13083970 Campinas, SP, BrazilUniv Estadual Campinas, CBMEG, Av Candido Rondon,400 Cidade Univ Zeferino Vaz, BR-13083875 Campinas, SP, BrazilUniv Florida, Dept Biol, Gainesville, FL USAUniv Estadual Paulista, Dept Bot, Inst Biociencias Botucatu, Rua Prof Dr Antonio Celso Wagner Zanin S-N, BR-18618689 Botucatu, SP, BrazilFundo de Apoio ao Ensino, a Pesquisa e Extensao-FAEPEX: 519.292Soc Botanica Sao PauloUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Univ FloridaVidal Jr, Joao de Deus [UNESP]Cortez, Maria BeatrizAlves, Fabio MatosKoehler, SamanthaSouza, Anete Pereira deKoch, Ingrid2018-11-26T17:54:59Z2018-11-26T17:54:59Z2018-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article681-686application/pdfhttp://dx.doi.org/10.1007/s40415-018-0465-9Brazilian Journal Of Botany. Sao Paulo: Soc Botanica Sao Paulo, v. 41, n. 3, p. 681-686, 2018.1806-9959http://hdl.handle.net/11449/16454810.1007/s40415-018-0465-9WOS:000442282500017WOS000442282500017.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Botanyinfo:eu-repo/semantics/openAccess2023-11-25T06:16:20Zoai:repositorio.unesp.br:11449/164548Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-25T06:16:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Development and cross-validation of microsatellite markers for Rauvolfia weddeliana Mull.Arg. (Apocynaceae) species complex |
title |
Development and cross-validation of microsatellite markers for Rauvolfia weddeliana Mull.Arg. (Apocynaceae) species complex |
spellingShingle |
Development and cross-validation of microsatellite markers for Rauvolfia weddeliana Mull.Arg. (Apocynaceae) species complex Vidal Jr, Joao de Deus [UNESP] Cerrado Conservation genetics Population genetics SSR |
title_short |
Development and cross-validation of microsatellite markers for Rauvolfia weddeliana Mull.Arg. (Apocynaceae) species complex |
title_full |
Development and cross-validation of microsatellite markers for Rauvolfia weddeliana Mull.Arg. (Apocynaceae) species complex |
title_fullStr |
Development and cross-validation of microsatellite markers for Rauvolfia weddeliana Mull.Arg. (Apocynaceae) species complex |
title_full_unstemmed |
Development and cross-validation of microsatellite markers for Rauvolfia weddeliana Mull.Arg. (Apocynaceae) species complex |
title_sort |
Development and cross-validation of microsatellite markers for Rauvolfia weddeliana Mull.Arg. (Apocynaceae) species complex |
author |
Vidal Jr, Joao de Deus [UNESP] |
author_facet |
Vidal Jr, Joao de Deus [UNESP] Cortez, Maria Beatriz Alves, Fabio Matos Koehler, Samantha Souza, Anete Pereira de Koch, Ingrid |
author_role |
author |
author2 |
Cortez, Maria Beatriz Alves, Fabio Matos Koehler, Samantha Souza, Anete Pereira de Koch, Ingrid |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Univ Florida |
dc.contributor.author.fl_str_mv |
Vidal Jr, Joao de Deus [UNESP] Cortez, Maria Beatriz Alves, Fabio Matos Koehler, Samantha Souza, Anete Pereira de Koch, Ingrid |
dc.subject.por.fl_str_mv |
Cerrado Conservation genetics Population genetics SSR |
topic |
Cerrado Conservation genetics Population genetics SSR |
description |
Rauvolfia weddeliana is an endemic species restricted to plateau landscapes in South American savannahs. Rapid loss of habitat and expansion of agriculture in Central-West Brazil have critically reduced the original extent of savannahs, representing a major threat to its biotic diversity. Due to the discontinuous distribution of R. weddeliana and the vulnerability of its habitats, it is crucial to estimate the genetic diversity of remaining populations. The application of microsatellite markers is a useful approach with relative low cost and high informative potential for studies related to conservation genetics and population genetics. The development of specific libraries for endangered species may aid future studies about the connectivity of populations, reproductive biology, and genetic diversity. We developed microsatellite markers for R. weddeliana and tested the transferability of the markers to a closely related species, R. gracilis. Ten microsatellite markers were identified, and a set of primers for their amplification is presented. Most identified motifs were dimers, with lengths from 18 to 74 base pairs. Nine markers presented high informative potential (PIC > 0.5). The set of markers developed in this study will support further investigations in population genetics of R. weddeliana and possibility of closely related species. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:54:59Z 2018-11-26T17:54:59Z 2018-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s40415-018-0465-9 Brazilian Journal Of Botany. Sao Paulo: Soc Botanica Sao Paulo, v. 41, n. 3, p. 681-686, 2018. 1806-9959 http://hdl.handle.net/11449/164548 10.1007/s40415-018-0465-9 WOS:000442282500017 WOS000442282500017.pdf |
url |
http://dx.doi.org/10.1007/s40415-018-0465-9 http://hdl.handle.net/11449/164548 |
identifier_str_mv |
Brazilian Journal Of Botany. Sao Paulo: Soc Botanica Sao Paulo, v. 41, n. 3, p. 681-686, 2018. 1806-9959 10.1007/s40415-018-0465-9 WOS:000442282500017 WOS000442282500017.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal Of Botany |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
681-686 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Botanica Sao Paulo |
publisher.none.fl_str_mv |
Soc Botanica Sao Paulo |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965066233118720 |