Expressions of matrix metalloproteinases-1 and-9 and opioid growth factor in rabbit cornea after lamellar keratectomy and treatment with 1% nalbuphine

Detalhes bibliográficos
Autor(a) principal: Silva, Miguel Ladino
Data de Publicação: 2015
Outros Autores: Ribeiro, Alexandre Pinto, Silva, Germana Alegro, Barbosa Sanchez, Irma Ximena, Renzo, Roberta, Uscategui, Ricardo, Lima, Tiago Barbalho, Aldrovani, Marcela, Laus, Jose Luiz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.5935/0004-2749.20150037
http://hdl.handle.net/11449/158450
Resumo: Purposes: To evaluate the effects of nalbuphine 1% on the expression of metalloproteinase 1 (MMP-1), metalloproteinase 9 (MMP-9), and opioid growth factor (OGF) in rabbit corneas after lamellar keratectomy. Methods: The rabbits were assigned to two groups: group nalbuphine (GN, n=30), which received 30 mu L of nalbuphine 1% in 4 daily applications at regular intervals until corneal epithelialization, and group control (GC, n=30), which received physiological saline solution under the same conditions adopted in GN. The corneas were collected for immunohistochemistry on days 1, 3, 5, 7, and 9 after lamellar keratectomy, and the expressions of MMP-1, MMP-9, and OGF were analyzed. Results: The expressions of MMP-1 and MMP-9 increased until day 5 of the evaluation, with no differences observed between GN and GC (p>0.05). On days 7 and 9, significant reductions were observed in the expression of MMP-1 (p<0.01), with no differences observed between GN and GC (p>0.05). The expression of OGF was constant in all periods (p>0.05), restricted to the corneal epithelium, and there was no difference between the groups (p>0.05). Conclusions: The study results showed that nalbuphine 1% did not alter the expression patterns of MMP-1, MMP-9, and OGF in rabbit corneas after lamellar keratectomy.
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spelling Expressions of matrix metalloproteinases-1 and-9 and opioid growth factor in rabbit cornea after lamellar keratectomy and treatment with 1% nalbuphineCorneaPhotorefractive keratectomyNalbuphine/therapeutic useMatrix metalloproteinasesReceptorsopioidImmunohistochemistryAnimalsRabbitsPurposes: To evaluate the effects of nalbuphine 1% on the expression of metalloproteinase 1 (MMP-1), metalloproteinase 9 (MMP-9), and opioid growth factor (OGF) in rabbit corneas after lamellar keratectomy. Methods: The rabbits were assigned to two groups: group nalbuphine (GN, n=30), which received 30 mu L of nalbuphine 1% in 4 daily applications at regular intervals until corneal epithelialization, and group control (GC, n=30), which received physiological saline solution under the same conditions adopted in GN. The corneas were collected for immunohistochemistry on days 1, 3, 5, 7, and 9 after lamellar keratectomy, and the expressions of MMP-1, MMP-9, and OGF were analyzed. Results: The expressions of MMP-1 and MMP-9 increased until day 5 of the evaluation, with no differences observed between GN and GC (p>0.05). On days 7 and 9, significant reductions were observed in the expression of MMP-1 (p<0.01), with no differences observed between GN and GC (p>0.05). The expression of OGF was constant in all periods (p>0.05), restricted to the corneal epithelium, and there was no difference between the groups (p>0.05). Conclusions: The study results showed that nalbuphine 1% did not alter the expression patterns of MMP-1, MMP-9, and OGF in rabbit corneas after lamellar keratectomy.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Sao Paulo, Dept Med & Surg, Coll Agr & Vet Sci, Jaboticabal, SP, BrazilLa Salle Univ, Coll Agr Sci, Bogota, ColombiaUniv Fed Mato Grosso, Coll Vet & Agr Sci, Dept Vet Clin Med, Cuiaba, MT, BrazilUniv Tolima, Coll Vet Med & Anim Sci, Ibague, ColombiaFAPESP: 2009/50082-8CNPq: 300833/2010-5Consel Brasil OftalmologiaUniversidade Estadual Paulista (Unesp)La Salle UnivUniversidade Federal de Mato Grosso do Sul (UFMS)Univ TolimaSilva, Miguel LadinoRibeiro, Alexandre PintoSilva, Germana AlegroBarbosa Sanchez, Irma XimenaRenzo, RobertaUscategui, RicardoLima, Tiago BarbalhoAldrovani, MarcelaLaus, Jose Luiz2018-11-26T15:27:42Z2018-11-26T15:27:42Z2015-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article141-145application/pdfhttp://dx.doi.org/10.5935/0004-2749.20150037Arquivos Brasileiros De Oftalmologia. Sao Paulo: Consel Brasil Oftalmologia, v. 78, n. 3, p. 141-145, 2015.0004-2749http://hdl.handle.net/11449/15845010.5935/0004-2749.20150037WOS:000358772000002WOS000358772000002.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivos Brasileiros De Oftalmologia0,518info:eu-repo/semantics/openAccess2024-01-09T06:25:56Zoai:repositorio.unesp.br:11449/158450Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:30:13.945976Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Expressions of matrix metalloproteinases-1 and-9 and opioid growth factor in rabbit cornea after lamellar keratectomy and treatment with 1% nalbuphine
title Expressions of matrix metalloproteinases-1 and-9 and opioid growth factor in rabbit cornea after lamellar keratectomy and treatment with 1% nalbuphine
spellingShingle Expressions of matrix metalloproteinases-1 and-9 and opioid growth factor in rabbit cornea after lamellar keratectomy and treatment with 1% nalbuphine
Silva, Miguel Ladino
Cornea
Photorefractive keratectomy
Nalbuphine/therapeutic use
Matrix metalloproteinases
Receptors
opioid
Immunohistochemistry
Animals
Rabbits
title_short Expressions of matrix metalloproteinases-1 and-9 and opioid growth factor in rabbit cornea after lamellar keratectomy and treatment with 1% nalbuphine
title_full Expressions of matrix metalloproteinases-1 and-9 and opioid growth factor in rabbit cornea after lamellar keratectomy and treatment with 1% nalbuphine
title_fullStr Expressions of matrix metalloproteinases-1 and-9 and opioid growth factor in rabbit cornea after lamellar keratectomy and treatment with 1% nalbuphine
title_full_unstemmed Expressions of matrix metalloproteinases-1 and-9 and opioid growth factor in rabbit cornea after lamellar keratectomy and treatment with 1% nalbuphine
title_sort Expressions of matrix metalloproteinases-1 and-9 and opioid growth factor in rabbit cornea after lamellar keratectomy and treatment with 1% nalbuphine
author Silva, Miguel Ladino
author_facet Silva, Miguel Ladino
Ribeiro, Alexandre Pinto
Silva, Germana Alegro
Barbosa Sanchez, Irma Ximena
Renzo, Roberta
Uscategui, Ricardo
Lima, Tiago Barbalho
Aldrovani, Marcela
Laus, Jose Luiz
author_role author
author2 Ribeiro, Alexandre Pinto
Silva, Germana Alegro
Barbosa Sanchez, Irma Ximena
Renzo, Roberta
Uscategui, Ricardo
Lima, Tiago Barbalho
Aldrovani, Marcela
Laus, Jose Luiz
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
La Salle Univ
Universidade Federal de Mato Grosso do Sul (UFMS)
Univ Tolima
dc.contributor.author.fl_str_mv Silva, Miguel Ladino
Ribeiro, Alexandre Pinto
Silva, Germana Alegro
Barbosa Sanchez, Irma Ximena
Renzo, Roberta
Uscategui, Ricardo
Lima, Tiago Barbalho
Aldrovani, Marcela
Laus, Jose Luiz
dc.subject.por.fl_str_mv Cornea
Photorefractive keratectomy
Nalbuphine/therapeutic use
Matrix metalloproteinases
Receptors
opioid
Immunohistochemistry
Animals
Rabbits
topic Cornea
Photorefractive keratectomy
Nalbuphine/therapeutic use
Matrix metalloproteinases
Receptors
opioid
Immunohistochemistry
Animals
Rabbits
description Purposes: To evaluate the effects of nalbuphine 1% on the expression of metalloproteinase 1 (MMP-1), metalloproteinase 9 (MMP-9), and opioid growth factor (OGF) in rabbit corneas after lamellar keratectomy. Methods: The rabbits were assigned to two groups: group nalbuphine (GN, n=30), which received 30 mu L of nalbuphine 1% in 4 daily applications at regular intervals until corneal epithelialization, and group control (GC, n=30), which received physiological saline solution under the same conditions adopted in GN. The corneas were collected for immunohistochemistry on days 1, 3, 5, 7, and 9 after lamellar keratectomy, and the expressions of MMP-1, MMP-9, and OGF were analyzed. Results: The expressions of MMP-1 and MMP-9 increased until day 5 of the evaluation, with no differences observed between GN and GC (p>0.05). On days 7 and 9, significant reductions were observed in the expression of MMP-1 (p<0.01), with no differences observed between GN and GC (p>0.05). The expression of OGF was constant in all periods (p>0.05), restricted to the corneal epithelium, and there was no difference between the groups (p>0.05). Conclusions: The study results showed that nalbuphine 1% did not alter the expression patterns of MMP-1, MMP-9, and OGF in rabbit corneas after lamellar keratectomy.
publishDate 2015
dc.date.none.fl_str_mv 2015-05-01
2018-11-26T15:27:42Z
2018-11-26T15:27:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.5935/0004-2749.20150037
Arquivos Brasileiros De Oftalmologia. Sao Paulo: Consel Brasil Oftalmologia, v. 78, n. 3, p. 141-145, 2015.
0004-2749
http://hdl.handle.net/11449/158450
10.5935/0004-2749.20150037
WOS:000358772000002
WOS000358772000002.pdf
url http://dx.doi.org/10.5935/0004-2749.20150037
http://hdl.handle.net/11449/158450
identifier_str_mv Arquivos Brasileiros De Oftalmologia. Sao Paulo: Consel Brasil Oftalmologia, v. 78, n. 3, p. 141-145, 2015.
0004-2749
10.5935/0004-2749.20150037
WOS:000358772000002
WOS000358772000002.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos Brasileiros De Oftalmologia
0,518
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 141-145
application/pdf
dc.publisher.none.fl_str_mv Consel Brasil Oftalmologia
publisher.none.fl_str_mv Consel Brasil Oftalmologia
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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