Influences of the alcoholic extract of Artemisia annua on gastrointestinal microbiota and performance of Nile tilapia
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.aquaculture.2022.738521 http://hdl.handle.net/11449/241238 |
Resumo: | There is growing interest in strategies for manipulating the gut microbiota of fish for beneficial communities to increase growth, improve lipid metabolism, stimulate the immune response, and improve the physiological state of the gut. We investigated the effects of diets supplemented with an alcoholic extract of Artemisia annua (ae-Aa) on gastrointestinal (GI) microbiota and intestinal morphology along with its effects on the growth performance of the Nile tilapia (Oreochromis niloticus). Juveniles were fed with ae-Aa supplementation (0.00, 0.10, 0.25, and 0.50% in the diets) for 30 days. The different ae-Aa supply levels affected bacterial interactions, resulting in a more complex network and increasing the proportion of positive correlations between bacterial groups. The different amounts of ae-Aa caused variations in specific bacterial abundance. The phyla Fusobacteriota and Bacteroidota were dominant in the intestinal microbiota in all experimental groups. The abundance of bacteria belonging to Fusobacteriaceae, Stenotrophomonas, and Clostridium correlated positively with protein efficiency, specific growth rate, weight gain, and final weight, while the opposite was observed for Acinetobacter. Diets supplemented with 0.25 and 0.5% of ae-Aa increased (p < 0.05) perimeter, height, and thickness of villi, crypt depth, villus:crypt ratio, and number of goblet cells in the intestine of Nile tilapia compared to control group. Nile tilapia supplemented with ae-Aa at all levels improved growth performance (weight gain, specific growth rate, and protein efficiency rate; p < 0.05) and feed conversion ratio (p < 0.05) compared to the control group. The weight gain was about 21% better in supplemented fish with ae-Aa than unsupplemented ones. The ae-Aa supplementation of Nile tilapia for 30 days modulated the GI microbiota communities providing variations in specific bacterial abundance, affecting the bacterial interactions, resulting in a more complex, stable, and connected network while changing their intestinal morphology and improving the performance of the fish. |
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Influences of the alcoholic extract of Artemisia annua on gastrointestinal microbiota and performance of Nile tilapiaArtemisiaGastrointestinal microbiotaGrowth promoterHerbal immunostimulantsIntestinal morphologyNatural additivesThere is growing interest in strategies for manipulating the gut microbiota of fish for beneficial communities to increase growth, improve lipid metabolism, stimulate the immune response, and improve the physiological state of the gut. We investigated the effects of diets supplemented with an alcoholic extract of Artemisia annua (ae-Aa) on gastrointestinal (GI) microbiota and intestinal morphology along with its effects on the growth performance of the Nile tilapia (Oreochromis niloticus). Juveniles were fed with ae-Aa supplementation (0.00, 0.10, 0.25, and 0.50% in the diets) for 30 days. The different ae-Aa supply levels affected bacterial interactions, resulting in a more complex network and increasing the proportion of positive correlations between bacterial groups. The different amounts of ae-Aa caused variations in specific bacterial abundance. The phyla Fusobacteriota and Bacteroidota were dominant in the intestinal microbiota in all experimental groups. The abundance of bacteria belonging to Fusobacteriaceae, Stenotrophomonas, and Clostridium correlated positively with protein efficiency, specific growth rate, weight gain, and final weight, while the opposite was observed for Acinetobacter. Diets supplemented with 0.25 and 0.5% of ae-Aa increased (p < 0.05) perimeter, height, and thickness of villi, crypt depth, villus:crypt ratio, and number of goblet cells in the intestine of Nile tilapia compared to control group. Nile tilapia supplemented with ae-Aa at all levels improved growth performance (weight gain, specific growth rate, and protein efficiency rate; p < 0.05) and feed conversion ratio (p < 0.05) compared to the control group. The weight gain was about 21% better in supplemented fish with ae-Aa than unsupplemented ones. The ae-Aa supplementation of Nile tilapia for 30 days modulated the GI microbiota communities providing variations in specific bacterial abundance, affecting the bacterial interactions, resulting in a more complex, stable, and connected network while changing their intestinal morphology and improving the performance of the fish.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Joint Graduate Program in Physiological Sciences Federal University of São Carlos-UFSCar/São Paulo State University, UNESP Campus AraraquaraGraduate Program in Animal Science State University of Maringá, UEM, Campus MaringáEmbrapa Environment Brazilian Agricultural Research Corporation (EMBRAPA), Rod. SP 340, Km 127,5, Caixa Postal 69Cellular and Molecular Biology Laboratory Center for Nuclear Energy in Agriculture (CENA) University of São Paulo (USP), SPChemical Biological and Agricultural Pluridisciplinary Research Center (CPQBA) Campinas State University (UNICAMP), SPJoint Graduate Program in Physiological Sciences Federal University of São Carlos-UFSCar/São Paulo State University, UNESP Campus AraraquaraUniversidade Estadual Paulista (UNESP)Universidade Estadual de Maringá (UEM)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade de São Paulo (USP)Universidade Estadual de Campinas (UNICAMP)Soares, Michelly Pereira [UNESP]Cardoso, Israel Luz [UNESP]Araújo, Fabrício EugênioDe Angelis, Carolina Fernandes [UNESP]Mendes, RodrigoMendes, Lucas WilliamFernandes, Marisa Narciso [UNESP]Jonsson, Claudio MartinSonia Claudiado Nascimento de, QueirozDuarte, Marta Cristina TeixeiraRantin, Francisco Tadeu [UNESP]Sampaio, Fernanda Garcia2023-03-01T20:52:59Z2023-03-01T20:52:59Z2022-11-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.aquaculture.2022.738521Aquaculture, v. 560.0044-8486http://hdl.handle.net/11449/24123810.1016/j.aquaculture.2022.7385212-s2.0-85132916744Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquacultureinfo:eu-repo/semantics/openAccess2023-03-01T20:52:59Zoai:repositorio.unesp.br:11449/241238Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:49:34.645194Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influences of the alcoholic extract of Artemisia annua on gastrointestinal microbiota and performance of Nile tilapia |
title |
Influences of the alcoholic extract of Artemisia annua on gastrointestinal microbiota and performance of Nile tilapia |
spellingShingle |
Influences of the alcoholic extract of Artemisia annua on gastrointestinal microbiota and performance of Nile tilapia Soares, Michelly Pereira [UNESP] Artemisia Gastrointestinal microbiota Growth promoter Herbal immunostimulants Intestinal morphology Natural additives |
title_short |
Influences of the alcoholic extract of Artemisia annua on gastrointestinal microbiota and performance of Nile tilapia |
title_full |
Influences of the alcoholic extract of Artemisia annua on gastrointestinal microbiota and performance of Nile tilapia |
title_fullStr |
Influences of the alcoholic extract of Artemisia annua on gastrointestinal microbiota and performance of Nile tilapia |
title_full_unstemmed |
Influences of the alcoholic extract of Artemisia annua on gastrointestinal microbiota and performance of Nile tilapia |
title_sort |
Influences of the alcoholic extract of Artemisia annua on gastrointestinal microbiota and performance of Nile tilapia |
author |
Soares, Michelly Pereira [UNESP] |
author_facet |
Soares, Michelly Pereira [UNESP] Cardoso, Israel Luz [UNESP] Araújo, Fabrício Eugênio De Angelis, Carolina Fernandes [UNESP] Mendes, Rodrigo Mendes, Lucas William Fernandes, Marisa Narciso [UNESP] Jonsson, Claudio Martin Sonia Claudiado Nascimento de, Queiroz Duarte, Marta Cristina Teixeira Rantin, Francisco Tadeu [UNESP] Sampaio, Fernanda Garcia |
author_role |
author |
author2 |
Cardoso, Israel Luz [UNESP] Araújo, Fabrício Eugênio De Angelis, Carolina Fernandes [UNESP] Mendes, Rodrigo Mendes, Lucas William Fernandes, Marisa Narciso [UNESP] Jonsson, Claudio Martin Sonia Claudiado Nascimento de, Queiroz Duarte, Marta Cristina Teixeira Rantin, Francisco Tadeu [UNESP] Sampaio, Fernanda Garcia |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Estadual de Maringá (UEM) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade de São Paulo (USP) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Soares, Michelly Pereira [UNESP] Cardoso, Israel Luz [UNESP] Araújo, Fabrício Eugênio De Angelis, Carolina Fernandes [UNESP] Mendes, Rodrigo Mendes, Lucas William Fernandes, Marisa Narciso [UNESP] Jonsson, Claudio Martin Sonia Claudiado Nascimento de, Queiroz Duarte, Marta Cristina Teixeira Rantin, Francisco Tadeu [UNESP] Sampaio, Fernanda Garcia |
dc.subject.por.fl_str_mv |
Artemisia Gastrointestinal microbiota Growth promoter Herbal immunostimulants Intestinal morphology Natural additives |
topic |
Artemisia Gastrointestinal microbiota Growth promoter Herbal immunostimulants Intestinal morphology Natural additives |
description |
There is growing interest in strategies for manipulating the gut microbiota of fish for beneficial communities to increase growth, improve lipid metabolism, stimulate the immune response, and improve the physiological state of the gut. We investigated the effects of diets supplemented with an alcoholic extract of Artemisia annua (ae-Aa) on gastrointestinal (GI) microbiota and intestinal morphology along with its effects on the growth performance of the Nile tilapia (Oreochromis niloticus). Juveniles were fed with ae-Aa supplementation (0.00, 0.10, 0.25, and 0.50% in the diets) for 30 days. The different ae-Aa supply levels affected bacterial interactions, resulting in a more complex network and increasing the proportion of positive correlations between bacterial groups. The different amounts of ae-Aa caused variations in specific bacterial abundance. The phyla Fusobacteriota and Bacteroidota were dominant in the intestinal microbiota in all experimental groups. The abundance of bacteria belonging to Fusobacteriaceae, Stenotrophomonas, and Clostridium correlated positively with protein efficiency, specific growth rate, weight gain, and final weight, while the opposite was observed for Acinetobacter. Diets supplemented with 0.25 and 0.5% of ae-Aa increased (p < 0.05) perimeter, height, and thickness of villi, crypt depth, villus:crypt ratio, and number of goblet cells in the intestine of Nile tilapia compared to control group. Nile tilapia supplemented with ae-Aa at all levels improved growth performance (weight gain, specific growth rate, and protein efficiency rate; p < 0.05) and feed conversion ratio (p < 0.05) compared to the control group. The weight gain was about 21% better in supplemented fish with ae-Aa than unsupplemented ones. The ae-Aa supplementation of Nile tilapia for 30 days modulated the GI microbiota communities providing variations in specific bacterial abundance, affecting the bacterial interactions, resulting in a more complex, stable, and connected network while changing their intestinal morphology and improving the performance of the fish. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-15 2023-03-01T20:52:59Z 2023-03-01T20:52:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.aquaculture.2022.738521 Aquaculture, v. 560. 0044-8486 http://hdl.handle.net/11449/241238 10.1016/j.aquaculture.2022.738521 2-s2.0-85132916744 |
url |
http://dx.doi.org/10.1016/j.aquaculture.2022.738521 http://hdl.handle.net/11449/241238 |
identifier_str_mv |
Aquaculture, v. 560. 0044-8486 10.1016/j.aquaculture.2022.738521 2-s2.0-85132916744 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquaculture |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129466338115584 |