Produção de glicose e frutose por invertase de Saccharomyces cerevisiae imobilizada em suporte MANAE-agarose

Detalhes bibliográficos
Autor(a) principal: Goulart, Antonio José [UNESP]
Data de Publicação: 2013
Outros Autores: Francisco dos Santos, Andréa [UNESP], Tavano, Olga Luisa, César Vinueza, Julio [UNESP], Contiero, Jonas [UNESP], Monti, Rubens [UNESP]
Tipo de documento: Artigo
Idioma: eng
por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/2570
http://hdl.handle.net/11449/75722
Resumo: Invertase from Saccharomyces cerevisiae was immobilized on agarose beads, activated with various groups (glyoxyl, MANAE or glutaraldehyde), and on some commercial epoxy supports (Eupergit and Sepabeads). Very active and stable invertase derivatives were produced by the adsorption of the enzyme on MANAE-agarose, MANAE-agarose treated with glutaraldhyde and glutaraldehyde-agarose supports. At pH 5.0, these derivatives retained full activity after 24h at 40°C and 50 °C. When assayed at 40°C and 50°C, with the pH adjusted to 7.0, the invertase-MANAE-agarose derivative treated with glutaraldehyde retained 80% of the initial activity. Recovered activities of the derivatives produced with MANAE, MANAE treated with glutaraldehyde and glutaraldehyde alone were 73.5%, 44.4% and 36.8%, respectively. These three preparations were successfully employed to produce glucose and fructose in 3 cycles of sucrose hydrolysis.
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spelling Produção de glicose e frutose por invertase de Saccharomyces cerevisiae imobilizada em suporte MANAE-agaroseGlucose and fructose production by Saccharomyces cerevisiae invertase immobilized on MANAE-agarose supportGlucose and fructose productionInvertaseInvertase immobilizationSaccharomyces cerevisiaeagarosebeta fructofuranosidaseepoxy resinfructoseglucoseglutaraldehydesucroseadsorptionbiotechnological productionenzyme activityenzyme assayenzyme immobilizationenzyme stabilityhydrolysiskineticsnonhumanpHtemperaturethermostabilityInvertase from Saccharomyces cerevisiae was immobilized on agarose beads, activated with various groups (glyoxyl, MANAE or glutaraldehyde), and on some commercial epoxy supports (Eupergit and Sepabeads). Very active and stable invertase derivatives were produced by the adsorption of the enzyme on MANAE-agarose, MANAE-agarose treated with glutaraldhyde and glutaraldehyde-agarose supports. At pH 5.0, these derivatives retained full activity after 24h at 40°C and 50 °C. When assayed at 40°C and 50°C, with the pH adjusted to 7.0, the invertase-MANAE-agarose derivative treated with glutaraldehyde retained 80% of the initial activity. Recovered activities of the derivatives produced with MANAE, MANAE treated with glutaraldehyde and glutaraldehyde alone were 73.5%, 44.4% and 36.8%, respectively. These three preparations were successfully employed to produce glucose and fructose in 3 cycles of sucrose hydrolysis.Department of Food and Nutrition Universidade Estadual Paulista (UNESP), Araraquara, SPDepartment of Biochemistry and Technological Chemistry Instituto de Química Universidade Estadual Paulista (UNESP), Araraquara, SPUniversidade Federal do Triangulo Mineiro, Uberaba, MGDepartment of Biochemistry and Microbiology Instituto de Biociências Universidade Estadual Paulista (UNESP), Rio Claro, SPDepartment of Food and Nutrition Universidade Estadual Paulista (UNESP), Araraquara, SPDepartment of Biochemistry and Technological Chemistry Instituto de Química Universidade Estadual Paulista (UNESP), Araraquara, SPDepartment of Biochemistry and Microbiology Instituto de Biociências Universidade Estadual Paulista (UNESP), Rio Claro, SPUniversidade Estadual Paulista (Unesp)Universidade Federal do Triângulo Mineiro (UFTM)Goulart, Antonio José [UNESP]Francisco dos Santos, Andréa [UNESP]Tavano, Olga LuisaCésar Vinueza, Julio [UNESP]Contiero, Jonas [UNESP]Monti, Rubens [UNESP]2014-05-27T11:29:47Z2014-05-27T11:29:47Z2013-06-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article169-175application/pdfhttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/2570Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 34, n. 2, p. 169-175, 2013.1808-4532http://hdl.handle.net/11449/757222-s2.0-848792549132-s2.0-84879254913.pdf9859154979447005Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporRevista de Ciências Farmacêuticas Básica e Aplicada0,131info:eu-repo/semantics/openAccess2024-06-21T12:46:30Zoai:repositorio.unesp.br:11449/75722Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:35:00.825240Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Produção de glicose e frutose por invertase de Saccharomyces cerevisiae imobilizada em suporte MANAE-agarose
Glucose and fructose production by Saccharomyces cerevisiae invertase immobilized on MANAE-agarose support
title Produção de glicose e frutose por invertase de Saccharomyces cerevisiae imobilizada em suporte MANAE-agarose
spellingShingle Produção de glicose e frutose por invertase de Saccharomyces cerevisiae imobilizada em suporte MANAE-agarose
Goulart, Antonio José [UNESP]
Glucose and fructose production
Invertase
Invertase immobilization
Saccharomyces cerevisiae
agarose
beta fructofuranosidase
epoxy resin
fructose
glucose
glutaraldehyde
sucrose
adsorption
biotechnological production
enzyme activity
enzyme assay
enzyme immobilization
enzyme stability
hydrolysis
kinetics
nonhuman
pH
temperature
thermostability
title_short Produção de glicose e frutose por invertase de Saccharomyces cerevisiae imobilizada em suporte MANAE-agarose
title_full Produção de glicose e frutose por invertase de Saccharomyces cerevisiae imobilizada em suporte MANAE-agarose
title_fullStr Produção de glicose e frutose por invertase de Saccharomyces cerevisiae imobilizada em suporte MANAE-agarose
title_full_unstemmed Produção de glicose e frutose por invertase de Saccharomyces cerevisiae imobilizada em suporte MANAE-agarose
title_sort Produção de glicose e frutose por invertase de Saccharomyces cerevisiae imobilizada em suporte MANAE-agarose
author Goulart, Antonio José [UNESP]
author_facet Goulart, Antonio José [UNESP]
Francisco dos Santos, Andréa [UNESP]
Tavano, Olga Luisa
César Vinueza, Julio [UNESP]
Contiero, Jonas [UNESP]
Monti, Rubens [UNESP]
author_role author
author2 Francisco dos Santos, Andréa [UNESP]
Tavano, Olga Luisa
César Vinueza, Julio [UNESP]
Contiero, Jonas [UNESP]
Monti, Rubens [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal do Triângulo Mineiro (UFTM)
dc.contributor.author.fl_str_mv Goulart, Antonio José [UNESP]
Francisco dos Santos, Andréa [UNESP]
Tavano, Olga Luisa
César Vinueza, Julio [UNESP]
Contiero, Jonas [UNESP]
Monti, Rubens [UNESP]
dc.subject.por.fl_str_mv Glucose and fructose production
Invertase
Invertase immobilization
Saccharomyces cerevisiae
agarose
beta fructofuranosidase
epoxy resin
fructose
glucose
glutaraldehyde
sucrose
adsorption
biotechnological production
enzyme activity
enzyme assay
enzyme immobilization
enzyme stability
hydrolysis
kinetics
nonhuman
pH
temperature
thermostability
topic Glucose and fructose production
Invertase
Invertase immobilization
Saccharomyces cerevisiae
agarose
beta fructofuranosidase
epoxy resin
fructose
glucose
glutaraldehyde
sucrose
adsorption
biotechnological production
enzyme activity
enzyme assay
enzyme immobilization
enzyme stability
hydrolysis
kinetics
nonhuman
pH
temperature
thermostability
description Invertase from Saccharomyces cerevisiae was immobilized on agarose beads, activated with various groups (glyoxyl, MANAE or glutaraldehyde), and on some commercial epoxy supports (Eupergit and Sepabeads). Very active and stable invertase derivatives were produced by the adsorption of the enzyme on MANAE-agarose, MANAE-agarose treated with glutaraldhyde and glutaraldehyde-agarose supports. At pH 5.0, these derivatives retained full activity after 24h at 40°C and 50 °C. When assayed at 40°C and 50°C, with the pH adjusted to 7.0, the invertase-MANAE-agarose derivative treated with glutaraldehyde retained 80% of the initial activity. Recovered activities of the derivatives produced with MANAE, MANAE treated with glutaraldehyde and glutaraldehyde alone were 73.5%, 44.4% and 36.8%, respectively. These three preparations were successfully employed to produce glucose and fructose in 3 cycles of sucrose hydrolysis.
publishDate 2013
dc.date.none.fl_str_mv 2013-06-27
2014-05-27T11:29:47Z
2014-05-27T11:29:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/2570
Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 34, n. 2, p. 169-175, 2013.
1808-4532
http://hdl.handle.net/11449/75722
2-s2.0-84879254913
2-s2.0-84879254913.pdf
9859154979447005
url http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/2570
http://hdl.handle.net/11449/75722
identifier_str_mv Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 34, n. 2, p. 169-175, 2013.
1808-4532
2-s2.0-84879254913
2-s2.0-84879254913.pdf
9859154979447005
dc.language.iso.fl_str_mv eng
por
language eng
por
dc.relation.none.fl_str_mv Revista de Ciências Farmacêuticas Básica e Aplicada
0,131
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 169-175
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128250200719360

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