Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0103-64402008000100007 http://hdl.handle.net/11449/212759 |
Resumo: | This study performed a histological analysis of the effect of formocresol associated to endotoxin (LPS) in the subcutaneous connective tissue of mice. Ninety mice were randomly assigned to 3 groups (n=30). Each animal received one plastic tube implant containing endotoxin solution (10 mg/mL), formocresol (original formula) or a mixture of endotoxin and formocresol. The endotoxin and formocresol groups served as controls. The periods of analysis were 7, 15 and 30 days. At each experimental period, tissue samples were collected and submitted to routine processing for histological analysis. Endotoxin and formocresol produced necrosis and chronic inflammation at 7 and 15 days. At 30 days, the endotoxin group showed no necrosis, while in the formocresol group necrosis persisted. The formocresol-endotoxin association produced necrosis and chronic inflammation in the same way as observed with formocresol at all experimental periods. In conclusion, formocresol seems not to be able to inactive the toxic effects of endotoxin in connective tissues. |
id |
UNSP_fb1cdb35f9ccec74195d2bb70a1d9e16 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/212759 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of miceformocresolendotoxinhistological analysismiceThis study performed a histological analysis of the effect of formocresol associated to endotoxin (LPS) in the subcutaneous connective tissue of mice. Ninety mice were randomly assigned to 3 groups (n=30). Each animal received one plastic tube implant containing endotoxin solution (10 mg/mL), formocresol (original formula) or a mixture of endotoxin and formocresol. The endotoxin and formocresol groups served as controls. The periods of analysis were 7, 15 and 30 days. At each experimental period, tissue samples were collected and submitted to routine processing for histological analysis. Endotoxin and formocresol produced necrosis and chronic inflammation at 7 and 15 days. At 30 days, the endotoxin group showed no necrosis, while in the formocresol group necrosis persisted. The formocresol-endotoxin association produced necrosis and chronic inflammation in the same way as observed with formocresol at all experimental periods. In conclusion, formocresol seems not to be able to inactive the toxic effects of endotoxin in connective tissues.O objetivo deste estudo foi avaliar histologicamente o efeito da associação do formocresol com endotoxina (LPS) em tecido conjuntivo de camundongos. Noventa camundongos foram divididos em três grupos de 30 camundongos cada. Cada camundongo recebeu um implante subcutâneo de tubo plástico contendo solução de endotoxina (10 mg/ml), formocresol (fórmula original), ou uma mistura de formocresol com endotoxina. Os grupos da endotoxina e formocresol foram considerados grupos controle. Os períodos de análise foram 7, 15 e 30 dias. Após os períodos experimentais, os tecidos foram removidos e submetidos a processamento histológico. Os resultados obtidos indicam que a endotoxina e o formocresol produzem necrose e inflamação tecidual crônica aos 7 e 15 dias e aos 30 dias o grupo da endotoxina não mostrava necrose e no grupo do formocresol a necrose persistiu. A combinação formocresol e endotoxina mostrou necrose e inflamação crônica com resultados semelhantes ao do grupo formocresol para todos os períodos experimentais. Pode-se concluir que o formocresol parece não ser capaz de inativar os efeitos tóxicos da endotoxina.Universidade Estadual Paulista, School of Dentistry of AraraquaraUniversidade Estadual Paulista, School of Dentistry of AraraquaraFundação Odontológica de Ribeirão PretoUniversidade Estadual Paulista (Unesp)Sant'anna, Ana Teresa [UNESP]Spolidório, Luis Carlos [UNESP]Ramalho, Lizeti Toledo Oliveira [UNESP]2021-07-14T10:44:44Z2021-07-14T10:44:44Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article40-45application/pdfhttp://dx.doi.org/10.1590/S0103-64402008000100007Brazilian Dental Journal. Ribeirão Preto, SP, Brazil: Fundação Odontológica de Ribeirão Preto, v. 19, n. 1, p. 40-45, 2008.0103-64401806-4760http://hdl.handle.net/11449/21275910.1590/S0103-64402008000100007S0103-64402008000100007S0103-64402008000100007.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journalinfo:eu-repo/semantics/openAccess2023-11-01T06:14:54Zoai:repositorio.unesp.br:11449/212759Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-01T06:14:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice |
title |
Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice |
spellingShingle |
Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice Sant'anna, Ana Teresa [UNESP] formocresol endotoxin histological analysis mice |
title_short |
Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice |
title_full |
Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice |
title_fullStr |
Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice |
title_full_unstemmed |
Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice |
title_sort |
Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice |
author |
Sant'anna, Ana Teresa [UNESP] |
author_facet |
Sant'anna, Ana Teresa [UNESP] Spolidório, Luis Carlos [UNESP] Ramalho, Lizeti Toledo Oliveira [UNESP] |
author_role |
author |
author2 |
Spolidório, Luis Carlos [UNESP] Ramalho, Lizeti Toledo Oliveira [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Sant'anna, Ana Teresa [UNESP] Spolidório, Luis Carlos [UNESP] Ramalho, Lizeti Toledo Oliveira [UNESP] |
dc.subject.por.fl_str_mv |
formocresol endotoxin histological analysis mice |
topic |
formocresol endotoxin histological analysis mice |
description |
This study performed a histological analysis of the effect of formocresol associated to endotoxin (LPS) in the subcutaneous connective tissue of mice. Ninety mice were randomly assigned to 3 groups (n=30). Each animal received one plastic tube implant containing endotoxin solution (10 mg/mL), formocresol (original formula) or a mixture of endotoxin and formocresol. The endotoxin and formocresol groups served as controls. The periods of analysis were 7, 15 and 30 days. At each experimental period, tissue samples were collected and submitted to routine processing for histological analysis. Endotoxin and formocresol produced necrosis and chronic inflammation at 7 and 15 days. At 30 days, the endotoxin group showed no necrosis, while in the formocresol group necrosis persisted. The formocresol-endotoxin association produced necrosis and chronic inflammation in the same way as observed with formocresol at all experimental periods. In conclusion, formocresol seems not to be able to inactive the toxic effects of endotoxin in connective tissues. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2021-07-14T10:44:44Z 2021-07-14T10:44:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0103-64402008000100007 Brazilian Dental Journal. Ribeirão Preto, SP, Brazil: Fundação Odontológica de Ribeirão Preto, v. 19, n. 1, p. 40-45, 2008. 0103-6440 1806-4760 http://hdl.handle.net/11449/212759 10.1590/S0103-64402008000100007 S0103-64402008000100007 S0103-64402008000100007.pdf |
url |
http://dx.doi.org/10.1590/S0103-64402008000100007 http://hdl.handle.net/11449/212759 |
identifier_str_mv |
Brazilian Dental Journal. Ribeirão Preto, SP, Brazil: Fundação Odontológica de Ribeirão Preto, v. 19, n. 1, p. 40-45, 2008. 0103-6440 1806-4760 10.1590/S0103-64402008000100007 S0103-64402008000100007 S0103-64402008000100007.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
40-45 application/pdf |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964785037541376 |