Three-dimensional printed poly (L-lactide) and hydroxyapatite composite for reconstruction of critical bone defect in rabbits
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/ACB360404 http://hdl.handle.net/11449/210391 |
Resumo: | Purpose: To use a 3D printed poly (L-lactide) acid (PLLA) and hydroxyapatite (HA) composite as a bone substitute for reconstruction of a critical bone defect in the radius of rabbits. Methods: A 1.5 cm ostectomy was performed in the radial diaphysis of 60 New Zealand white rabbits. The rabbits were divided into three groups according to surgical treatment of the bone defect (group I - control, group II - bone graft, group III - 3D PLLA). Each group was divided into four subgroups with different radiographic and histopathologic evaluation times (T1 - 15 days, T2 - 30 days, T3 - 60 days, T4 - 90 days). Results: The implant group had greater clinically lameness (p = 0.02), edema (p = 0.007), pain (p = 0.04) and more complications at the surgical site (p = 0.03). Histologically, this group showed greater congestion (p = 0.04), hemorrhage (p = 0.04) and inflammation. Osteogenesis was microscopically similar between days (p = 0.54) and treatments (p = 0.17), even though radiographically, more effective bone healing occurred in the graft group (II), with more callus and bone bridge formation. Conclusions: The customization of a 3D PLLA/HA scaffold was successful. However, in animals receiving the polymer-ceramic composite less bone callus and bone bridge was formed compared to the graft group. |
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Repositório Institucional da UNESP |
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Three-dimensional printed poly (L-lactide) and hydroxyapatite composite for reconstruction of critical bone defect in rabbitsBiocompatible MaterialsOsteogenesisTissue ScaffoldsTissue EngineeringRabbitsPurpose: To use a 3D printed poly (L-lactide) acid (PLLA) and hydroxyapatite (HA) composite as a bone substitute for reconstruction of a critical bone defect in the radius of rabbits. Methods: A 1.5 cm ostectomy was performed in the radial diaphysis of 60 New Zealand white rabbits. The rabbits were divided into three groups according to surgical treatment of the bone defect (group I - control, group II - bone graft, group III - 3D PLLA). Each group was divided into four subgroups with different radiographic and histopathologic evaluation times (T1 - 15 days, T2 - 30 days, T3 - 60 days, T4 - 90 days). Results: The implant group had greater clinically lameness (p = 0.02), edema (p = 0.007), pain (p = 0.04) and more complications at the surgical site (p = 0.03). Histologically, this group showed greater congestion (p = 0.04), hemorrhage (p = 0.04) and inflammation. Osteogenesis was microscopically similar between days (p = 0.54) and treatments (p = 0.17), even though radiographically, more effective bone healing occurred in the graft group (II), with more callus and bone bridge formation. Conclusions: The customization of a 3D PLLA/HA scaffold was successful. However, in animals receiving the polymer-ceramic composite less bone callus and bone bridge was formed compared to the graft group.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Fac Agr & Vet Sci, Dept Clin & Vet Surg, Jaboticabal, SP, BrazilCtr Univ Maringa, Dept Vet Anat, Maringa, Parana, BrazilUniv Estadual Paulista, Fac Agr & Vet Sci, Grad Program Vet Surg, Jaboticabal, SP, BrazilUniv Brasil, Dept Clin & Vet Surg, Descalvado, SP, BrazilCtr Univ Maringa, Dept Vet Surg, Maringa, Parana, BrazilCtr Univ Maringa, Maringa, Parana, BrazilUniv Estadual Paulista, Fac Agr & Vet Sci, Dept Vet Clin Sci, Botucatu, SP, BrazilUniv Fed Vales Jequitinhonha & Mucuri, Inst Sci, Dept Vet Clin Sci, Unai, MG, BrazilUniv Estadual Paulista Julio de Mesquita Filho UN, Fac Agr & Vet Sci, Dept Clin & Vet Surg, Jaboticabal, SP, BrazilUniv Estadual Paulista, Fac Agr & Vet Sci, Dept Clin & Vet Surg, Jaboticabal, SP, BrazilUniv Estadual Paulista, Fac Agr & Vet Sci, Grad Program Vet Surg, Jaboticabal, SP, BrazilUniv Estadual Paulista, Fac Agr & Vet Sci, Dept Vet Clin Sci, Botucatu, SP, BrazilUniv Estadual Paulista Julio de Mesquita Filho UN, Fac Agr & Vet Sci, Dept Clin & Vet Surg, Jaboticabal, SP, BrazilFAPESP: 2015/10139-1Acta Cirurgica BrasileiraUniversidade Estadual Paulista (Unesp)Ctr Univ MaringaUniv BrasilUniversidade Federal de Viçosa (UFV)Minto, Bruno Watanabe [UNESP]Sprada, Aricia Gomes [UNESP]Goncalves Neto, Jose Aloizio [UNESP]Alcantara, Brenda Mendonca de [UNESP]Sa Rocha, Thiago Andre Salvitti de [UNESP]Valentim Hespanha, Ana Carolina [UNESP]Quarterone, Carolina [UNESP]Sartori, Maressa da Rocha [UNESP]Hataka, Alessandre [UNESP]Ramirez Uscategui, Ricardo Andres [UNESP]Gosuen Goncalves Dias, Luis Gustavo [UNESP]2021-06-25T15:07:00Z2021-06-25T15:07:00Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://dx.doi.org/10.1590/ACB360404Acta Cirurgica Brasileira. Sao Paulo: Acta Cirurgica Brasileira, v. 36, n. 4, 9 p., 2021.0102-8650http://hdl.handle.net/11449/21039110.1590/ACB360404S0102-86502021000400203WOS:000657155200001S0102-86502021000400203.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccess2024-06-06T14:09:53Zoai:repositorio.unesp.br:11449/210391Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:06:09.812426Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Three-dimensional printed poly (L-lactide) and hydroxyapatite composite for reconstruction of critical bone defect in rabbits |
title |
Three-dimensional printed poly (L-lactide) and hydroxyapatite composite for reconstruction of critical bone defect in rabbits |
spellingShingle |
Three-dimensional printed poly (L-lactide) and hydroxyapatite composite for reconstruction of critical bone defect in rabbits Minto, Bruno Watanabe [UNESP] Biocompatible Materials Osteogenesis Tissue Scaffolds Tissue Engineering Rabbits |
title_short |
Three-dimensional printed poly (L-lactide) and hydroxyapatite composite for reconstruction of critical bone defect in rabbits |
title_full |
Three-dimensional printed poly (L-lactide) and hydroxyapatite composite for reconstruction of critical bone defect in rabbits |
title_fullStr |
Three-dimensional printed poly (L-lactide) and hydroxyapatite composite for reconstruction of critical bone defect in rabbits |
title_full_unstemmed |
Three-dimensional printed poly (L-lactide) and hydroxyapatite composite for reconstruction of critical bone defect in rabbits |
title_sort |
Three-dimensional printed poly (L-lactide) and hydroxyapatite composite for reconstruction of critical bone defect in rabbits |
author |
Minto, Bruno Watanabe [UNESP] |
author_facet |
Minto, Bruno Watanabe [UNESP] Sprada, Aricia Gomes [UNESP] Goncalves Neto, Jose Aloizio [UNESP] Alcantara, Brenda Mendonca de [UNESP] Sa Rocha, Thiago Andre Salvitti de [UNESP] Valentim Hespanha, Ana Carolina [UNESP] Quarterone, Carolina [UNESP] Sartori, Maressa da Rocha [UNESP] Hataka, Alessandre [UNESP] Ramirez Uscategui, Ricardo Andres [UNESP] Gosuen Goncalves Dias, Luis Gustavo [UNESP] |
author_role |
author |
author2 |
Sprada, Aricia Gomes [UNESP] Goncalves Neto, Jose Aloizio [UNESP] Alcantara, Brenda Mendonca de [UNESP] Sa Rocha, Thiago Andre Salvitti de [UNESP] Valentim Hespanha, Ana Carolina [UNESP] Quarterone, Carolina [UNESP] Sartori, Maressa da Rocha [UNESP] Hataka, Alessandre [UNESP] Ramirez Uscategui, Ricardo Andres [UNESP] Gosuen Goncalves Dias, Luis Gustavo [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Ctr Univ Maringa Univ Brasil Universidade Federal de Viçosa (UFV) |
dc.contributor.author.fl_str_mv |
Minto, Bruno Watanabe [UNESP] Sprada, Aricia Gomes [UNESP] Goncalves Neto, Jose Aloizio [UNESP] Alcantara, Brenda Mendonca de [UNESP] Sa Rocha, Thiago Andre Salvitti de [UNESP] Valentim Hespanha, Ana Carolina [UNESP] Quarterone, Carolina [UNESP] Sartori, Maressa da Rocha [UNESP] Hataka, Alessandre [UNESP] Ramirez Uscategui, Ricardo Andres [UNESP] Gosuen Goncalves Dias, Luis Gustavo [UNESP] |
dc.subject.por.fl_str_mv |
Biocompatible Materials Osteogenesis Tissue Scaffolds Tissue Engineering Rabbits |
topic |
Biocompatible Materials Osteogenesis Tissue Scaffolds Tissue Engineering Rabbits |
description |
Purpose: To use a 3D printed poly (L-lactide) acid (PLLA) and hydroxyapatite (HA) composite as a bone substitute for reconstruction of a critical bone defect in the radius of rabbits. Methods: A 1.5 cm ostectomy was performed in the radial diaphysis of 60 New Zealand white rabbits. The rabbits were divided into three groups according to surgical treatment of the bone defect (group I - control, group II - bone graft, group III - 3D PLLA). Each group was divided into four subgroups with different radiographic and histopathologic evaluation times (T1 - 15 days, T2 - 30 days, T3 - 60 days, T4 - 90 days). Results: The implant group had greater clinically lameness (p = 0.02), edema (p = 0.007), pain (p = 0.04) and more complications at the surgical site (p = 0.03). Histologically, this group showed greater congestion (p = 0.04), hemorrhage (p = 0.04) and inflammation. Osteogenesis was microscopically similar between days (p = 0.54) and treatments (p = 0.17), even though radiographically, more effective bone healing occurred in the graft group (II), with more callus and bone bridge formation. Conclusions: The customization of a 3D PLLA/HA scaffold was successful. However, in animals receiving the polymer-ceramic composite less bone callus and bone bridge was formed compared to the graft group. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T15:07:00Z 2021-06-25T15:07:00Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/ACB360404 Acta Cirurgica Brasileira. Sao Paulo: Acta Cirurgica Brasileira, v. 36, n. 4, 9 p., 2021. 0102-8650 http://hdl.handle.net/11449/210391 10.1590/ACB360404 S0102-86502021000400203 WOS:000657155200001 S0102-86502021000400203.pdf |
url |
http://dx.doi.org/10.1590/ACB360404 http://hdl.handle.net/11449/210391 |
identifier_str_mv |
Acta Cirurgica Brasileira. Sao Paulo: Acta Cirurgica Brasileira, v. 36, n. 4, 9 p., 2021. 0102-8650 10.1590/ACB360404 S0102-86502021000400203 WOS:000657155200001 S0102-86502021000400203.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Cirurgica Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 application/pdf |
dc.publisher.none.fl_str_mv |
Acta Cirurgica Brasileira |
publisher.none.fl_str_mv |
Acta Cirurgica Brasileira |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128755703480320 |