Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/jbm.b.34586 http://hdl.handle.net/11449/200070 |
Resumo: | The aim of this study was to develop a highly porous calcium-containing chitosan scaffold suitable for dentin regeneration. A calcium hydroxide (Ca[OH]2) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds. The chitosan solution concentration and freezing protocol were adjusted to optimize the porous architecture using the phase-separation technique. Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network. Scaffolds were cross-linked on glutaraldehyde (GA) vapor. Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%). Fourier-transform infrared spectroscopy indicated the establishment of Schiff base on cross-linked scaffolds, along with calcium complexation for CH-Ca. Cross-linked CH-Ca scaffold featured a sustained Ca2+ release up to 21 days in a humid environment. This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters. HDPCs seeded onto CH-Ca featured significantly higher ALP activity, and gene expressions for ALP, Col1, DMP-1, and DSPP in comparison to CH, leading to a significant 3.5 times increase in calcium-rich matrix deposition. In sum, our findings suggest that CH-Ca scaffolds are attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering. |
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Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineeringcalcium hydroxidechitosandental pulpdentinporous scaffoldsThe aim of this study was to develop a highly porous calcium-containing chitosan scaffold suitable for dentin regeneration. A calcium hydroxide (Ca[OH]2) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds. The chitosan solution concentration and freezing protocol were adjusted to optimize the porous architecture using the phase-separation technique. Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network. Scaffolds were cross-linked on glutaraldehyde (GA) vapor. Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%). Fourier-transform infrared spectroscopy indicated the establishment of Schiff base on cross-linked scaffolds, along with calcium complexation for CH-Ca. Cross-linked CH-Ca scaffold featured a sustained Ca2+ release up to 21 days in a humid environment. This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters. HDPCs seeded onto CH-Ca featured significantly higher ALP activity, and gene expressions for ALP, Col1, DMP-1, and DSPP in comparison to CH, leading to a significant 3.5 times increase in calcium-rich matrix deposition. In sum, our findings suggest that CH-Ca scaffolds are attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry Sao Paulo University - USPDepartment of Physiology and Pathology Araraquara School of Dentistry Univ. Estadual Paulista - UNESPDepartment of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry Univ. Estadual Paulista – UNESPDepartment of Physics School of Sciences Univ. Estadual Paulista – UNESPDepartment of Cariology Restorative Sciences Endodontics School of Dentistry University of MichiganDepartment of Physiology and Pathology Araraquara School of Dentistry Univ. Estadual Paulista - UNESPDepartment of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry Univ. Estadual Paulista – UNESPDepartment of Physics School of Sciences Univ. Estadual Paulista – UNESPCAPES: 001FAPESP: 2016/15674-5Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)University of MichiganSoares, Diana GabrielaBordini, Ester Alves Ferreira [UNESP]Cassiano, Fernanda Balestrero [UNESP]Bronze-Uhle, Erika SoaresPacheco, Leandro EdgarZabeo, GiovanaHebling, Josimeri [UNESP]Lisboa-Filho, Paulo Noronha [UNESP]Bottino, Marco Cicerode Souza Costa, Carlos Alberto [UNESP]2020-12-12T01:56:53Z2020-12-12T01:56:53Z2020-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2546-2559http://dx.doi.org/10.1002/jbm.b.34586Journal of Biomedical Materials Research - Part B Applied Biomaterials, v. 108, n. 6, p. 2546-2559, 2020.1552-49811552-4973http://hdl.handle.net/11449/20007010.1002/jbm.b.345862-s2.0-85079454970Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Biomedical Materials Research - Part B Applied Biomaterialsinfo:eu-repo/semantics/openAccess2021-10-23T11:51:54Zoai:repositorio.unesp.br:11449/200070Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T11:51:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering |
title |
Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering |
spellingShingle |
Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering Soares, Diana Gabriela calcium hydroxide chitosan dental pulp dentin porous scaffolds |
title_short |
Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering |
title_full |
Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering |
title_fullStr |
Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering |
title_full_unstemmed |
Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering |
title_sort |
Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering |
author |
Soares, Diana Gabriela |
author_facet |
Soares, Diana Gabriela Bordini, Ester Alves Ferreira [UNESP] Cassiano, Fernanda Balestrero [UNESP] Bronze-Uhle, Erika Soares Pacheco, Leandro Edgar Zabeo, Giovana Hebling, Josimeri [UNESP] Lisboa-Filho, Paulo Noronha [UNESP] Bottino, Marco Cicero de Souza Costa, Carlos Alberto [UNESP] |
author_role |
author |
author2 |
Bordini, Ester Alves Ferreira [UNESP] Cassiano, Fernanda Balestrero [UNESP] Bronze-Uhle, Erika Soares Pacheco, Leandro Edgar Zabeo, Giovana Hebling, Josimeri [UNESP] Lisboa-Filho, Paulo Noronha [UNESP] Bottino, Marco Cicero de Souza Costa, Carlos Alberto [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) University of Michigan |
dc.contributor.author.fl_str_mv |
Soares, Diana Gabriela Bordini, Ester Alves Ferreira [UNESP] Cassiano, Fernanda Balestrero [UNESP] Bronze-Uhle, Erika Soares Pacheco, Leandro Edgar Zabeo, Giovana Hebling, Josimeri [UNESP] Lisboa-Filho, Paulo Noronha [UNESP] Bottino, Marco Cicero de Souza Costa, Carlos Alberto [UNESP] |
dc.subject.por.fl_str_mv |
calcium hydroxide chitosan dental pulp dentin porous scaffolds |
topic |
calcium hydroxide chitosan dental pulp dentin porous scaffolds |
description |
The aim of this study was to develop a highly porous calcium-containing chitosan scaffold suitable for dentin regeneration. A calcium hydroxide (Ca[OH]2) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds. The chitosan solution concentration and freezing protocol were adjusted to optimize the porous architecture using the phase-separation technique. Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network. Scaffolds were cross-linked on glutaraldehyde (GA) vapor. Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%). Fourier-transform infrared spectroscopy indicated the establishment of Schiff base on cross-linked scaffolds, along with calcium complexation for CH-Ca. Cross-linked CH-Ca scaffold featured a sustained Ca2+ release up to 21 days in a humid environment. This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters. HDPCs seeded onto CH-Ca featured significantly higher ALP activity, and gene expressions for ALP, Col1, DMP-1, and DSPP in comparison to CH, leading to a significant 3.5 times increase in calcium-rich matrix deposition. In sum, our findings suggest that CH-Ca scaffolds are attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:56:53Z 2020-12-12T01:56:53Z 2020-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/jbm.b.34586 Journal of Biomedical Materials Research - Part B Applied Biomaterials, v. 108, n. 6, p. 2546-2559, 2020. 1552-4981 1552-4973 http://hdl.handle.net/11449/200070 10.1002/jbm.b.34586 2-s2.0-85079454970 |
url |
http://dx.doi.org/10.1002/jbm.b.34586 http://hdl.handle.net/11449/200070 |
identifier_str_mv |
Journal of Biomedical Materials Research - Part B Applied Biomaterials, v. 108, n. 6, p. 2546-2559, 2020. 1552-4981 1552-4973 10.1002/jbm.b.34586 2-s2.0-85079454970 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Biomedical Materials Research - Part B Applied Biomaterials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2546-2559 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964590109360128 |