Expression of differential genes involved in the maintenance of water balance in human skin by Piptadenia colubrina extract
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/j.1473-2165.2009.00458.x http://hdl.handle.net/11449/225962 |
Resumo: | Background: Hydration and integrity of the stratum corneum (SC) is an important determinant of skin appearance, metabolism, mechanical properties, and barrier function. The presence of aquaglyceroporins and envelope proteins are crucial to provide greater corneocyte cohesion to keep water and other moisturizers in the skin. Aims: In this study, we evaluated the ability of Piptadenia colubrina, a plant native of South American rain forests, in the expression of genes involved in skin capacitance and SC integrity. Methods: The expression of genes for aquaporin-3 (AQP3), loricrin, involucrin (INV), and filaggrin (FLG) was measured by real-time PCR, using an in vitro model of human keratinocytes incubated with concentrations of 2.5, 5, 10, and 20 mg/mL of a hydroglycolic extract of P. colubrina (HEPC). The amount of AQP3 protein was also tested by immunohistochemistry in human skin explants. Clinical trials were conducted to evaluate the effects of a gel-cream containing HEPC on the glycerol index and skin capacitance. Results: Hydroglycolic extract of P. colubrina increased both the expression and immunoreactivity of AQP3 in cultured keratinocytes and human skin explants. The gene induction to envelope proteins FLG and INV was also observed after cell incubation with HEPC. Skin capacitance was significantly improved in human volunteers under treatment with HEPC-containing cream. Conclusions: The extract of P. colubrina promotes cellular hydration and induces gene expression of envelope proteins providing greater corneocyte cohesion to keep water and other moisturizers in the skin and an appropriate epidermal adhesion. The in vitro findings were clinically confirmed and encourage the clinical use of this compound in skin care products. © 2010 Wiley Periodicals, Inc. |
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Repositório Institucional da UNESP |
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Expression of differential genes involved in the maintenance of water balance in human skin by Piptadenia colubrina extractAquaporin-3Cornified envelopeFilaggrinGlycerolInvolucrinPiptadenia colubrinaBackground: Hydration and integrity of the stratum corneum (SC) is an important determinant of skin appearance, metabolism, mechanical properties, and barrier function. The presence of aquaglyceroporins and envelope proteins are crucial to provide greater corneocyte cohesion to keep water and other moisturizers in the skin. Aims: In this study, we evaluated the ability of Piptadenia colubrina, a plant native of South American rain forests, in the expression of genes involved in skin capacitance and SC integrity. Methods: The expression of genes for aquaporin-3 (AQP3), loricrin, involucrin (INV), and filaggrin (FLG) was measured by real-time PCR, using an in vitro model of human keratinocytes incubated with concentrations of 2.5, 5, 10, and 20 mg/mL of a hydroglycolic extract of P. colubrina (HEPC). The amount of AQP3 protein was also tested by immunohistochemistry in human skin explants. Clinical trials were conducted to evaluate the effects of a gel-cream containing HEPC on the glycerol index and skin capacitance. Results: Hydroglycolic extract of P. colubrina increased both the expression and immunoreactivity of AQP3 in cultured keratinocytes and human skin explants. The gene induction to envelope proteins FLG and INV was also observed after cell incubation with HEPC. Skin capacitance was significantly improved in human volunteers under treatment with HEPC-containing cream. Conclusions: The extract of P. colubrina promotes cellular hydration and induces gene expression of envelope proteins providing greater corneocyte cohesion to keep water and other moisturizers in the skin and an appropriate epidermal adhesion. The in vitro findings were clinically confirmed and encourage the clinical use of this compound in skin care products. © 2010 Wiley Periodicals, Inc.Department of Pharmacology/Hemocenter University of Campinas (UNICAMP), CampinasResearch and Development Department Chemyunion Química Ltda, SorocabaDepartment of Genetics São Paulo State University (UNESP), BotucatuDepartment of Pharmacology São Paulo State University (UNESP), BotucatuDepartment of Genetics São Paulo State University (UNESP), BotucatuDepartment of Pharmacology São Paulo State University (UNESP), BotucatuUniversidade Estadual de Campinas (UNICAMP)Chemyunion Química LtdaUniversidade Estadual Paulista (UNESP)Del Carmen Velazquez Pereda, Mariade Campos Dieamant, GustavoEberlin, SamaraWerka, Rejane MariaColombi, Débora [UNESP]de Souza Queiroz, Mary LuciDi Stasi, Luiz Claudio [UNESP]2022-04-28T21:02:10Z2022-04-28T21:02:10Z2010-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article35-43http://dx.doi.org/10.1111/j.1473-2165.2009.00458.xJournal of Cosmetic Dermatology, v. 9, n. 1, p. 35-43, 2010.1473-21301473-2165http://hdl.handle.net/11449/22596210.1111/j.1473-2165.2009.00458.x2-s2.0-77954381159Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Cosmetic Dermatologyinfo:eu-repo/semantics/openAccess2022-04-28T21:02:10Zoai:repositorio.unesp.br:11449/225962Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:12:15.686376Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Expression of differential genes involved in the maintenance of water balance in human skin by Piptadenia colubrina extract |
title |
Expression of differential genes involved in the maintenance of water balance in human skin by Piptadenia colubrina extract |
spellingShingle |
Expression of differential genes involved in the maintenance of water balance in human skin by Piptadenia colubrina extract Del Carmen Velazquez Pereda, Maria Aquaporin-3 Cornified envelope Filaggrin Glycerol Involucrin Piptadenia colubrina |
title_short |
Expression of differential genes involved in the maintenance of water balance in human skin by Piptadenia colubrina extract |
title_full |
Expression of differential genes involved in the maintenance of water balance in human skin by Piptadenia colubrina extract |
title_fullStr |
Expression of differential genes involved in the maintenance of water balance in human skin by Piptadenia colubrina extract |
title_full_unstemmed |
Expression of differential genes involved in the maintenance of water balance in human skin by Piptadenia colubrina extract |
title_sort |
Expression of differential genes involved in the maintenance of water balance in human skin by Piptadenia colubrina extract |
author |
Del Carmen Velazquez Pereda, Maria |
author_facet |
Del Carmen Velazquez Pereda, Maria de Campos Dieamant, Gustavo Eberlin, Samara Werka, Rejane Maria Colombi, Débora [UNESP] de Souza Queiroz, Mary Luci Di Stasi, Luiz Claudio [UNESP] |
author_role |
author |
author2 |
de Campos Dieamant, Gustavo Eberlin, Samara Werka, Rejane Maria Colombi, Débora [UNESP] de Souza Queiroz, Mary Luci Di Stasi, Luiz Claudio [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Chemyunion Química Ltda Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Del Carmen Velazquez Pereda, Maria de Campos Dieamant, Gustavo Eberlin, Samara Werka, Rejane Maria Colombi, Débora [UNESP] de Souza Queiroz, Mary Luci Di Stasi, Luiz Claudio [UNESP] |
dc.subject.por.fl_str_mv |
Aquaporin-3 Cornified envelope Filaggrin Glycerol Involucrin Piptadenia colubrina |
topic |
Aquaporin-3 Cornified envelope Filaggrin Glycerol Involucrin Piptadenia colubrina |
description |
Background: Hydration and integrity of the stratum corneum (SC) is an important determinant of skin appearance, metabolism, mechanical properties, and barrier function. The presence of aquaglyceroporins and envelope proteins are crucial to provide greater corneocyte cohesion to keep water and other moisturizers in the skin. Aims: In this study, we evaluated the ability of Piptadenia colubrina, a plant native of South American rain forests, in the expression of genes involved in skin capacitance and SC integrity. Methods: The expression of genes for aquaporin-3 (AQP3), loricrin, involucrin (INV), and filaggrin (FLG) was measured by real-time PCR, using an in vitro model of human keratinocytes incubated with concentrations of 2.5, 5, 10, and 20 mg/mL of a hydroglycolic extract of P. colubrina (HEPC). The amount of AQP3 protein was also tested by immunohistochemistry in human skin explants. Clinical trials were conducted to evaluate the effects of a gel-cream containing HEPC on the glycerol index and skin capacitance. Results: Hydroglycolic extract of P. colubrina increased both the expression and immunoreactivity of AQP3 in cultured keratinocytes and human skin explants. The gene induction to envelope proteins FLG and INV was also observed after cell incubation with HEPC. Skin capacitance was significantly improved in human volunteers under treatment with HEPC-containing cream. Conclusions: The extract of P. colubrina promotes cellular hydration and induces gene expression of envelope proteins providing greater corneocyte cohesion to keep water and other moisturizers in the skin and an appropriate epidermal adhesion. The in vitro findings were clinically confirmed and encourage the clinical use of this compound in skin care products. © 2010 Wiley Periodicals, Inc. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-03-01 2022-04-28T21:02:10Z 2022-04-28T21:02:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/j.1473-2165.2009.00458.x Journal of Cosmetic Dermatology, v. 9, n. 1, p. 35-43, 2010. 1473-2130 1473-2165 http://hdl.handle.net/11449/225962 10.1111/j.1473-2165.2009.00458.x 2-s2.0-77954381159 |
url |
http://dx.doi.org/10.1111/j.1473-2165.2009.00458.x http://hdl.handle.net/11449/225962 |
identifier_str_mv |
Journal of Cosmetic Dermatology, v. 9, n. 1, p. 35-43, 2010. 1473-2130 1473-2165 10.1111/j.1473-2165.2009.00458.x 2-s2.0-77954381159 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Cosmetic Dermatology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
35-43 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128907822497792 |