An Anopheles aquasalis GATA factor Serpent is required for immunity against Plasmodium and bacteria
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pntd.0006785 http://hdl.handle.net/11449/186928 |
Resumo: | Innate immunity is an ancient and conserved defense system that provides an early effective response against invaders. Many immune genes of Anopheles mosquitoes have been implicated in defense against a variety of pathogens, including plasmodia. Nevertheless, only recent work identified some immune genes of Anopheles aquasalis mosquitoes upon P. vivax infection. Among these was a GATA transcription factor gene, which is described here. This is an ortholog of GATA factor Serpent genes described in Drosophila melanogaster and Anopheles gambiae. Gene expression analyses showed an increase of GATA-Serpent mRNA in P. vivax-infected A. aquasalis and functional RNAi experiments identified this transcription factor as an important immune gene of A. aquasalis against both bacteria and P. vivax. Besides, we were able to identify an effect of GATA-Serpent knockdown on A. aquasalis hemocyte proliferation and differentiation. These findings expand our understanding of the poorly studied A. aquasalis-P. vivax interactions and uncover GATA-Serpent as a key player of the mosquito innate immune response. |
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An Anopheles aquasalis GATA factor Serpent is required for immunity against Plasmodium and bacteriaInnate immunity is an ancient and conserved defense system that provides an early effective response against invaders. Many immune genes of Anopheles mosquitoes have been implicated in defense against a variety of pathogens, including plasmodia. Nevertheless, only recent work identified some immune genes of Anopheles aquasalis mosquitoes upon P. vivax infection. Among these was a GATA transcription factor gene, which is described here. This is an ortholog of GATA factor Serpent genes described in Drosophila melanogaster and Anopheles gambiae. Gene expression analyses showed an increase of GATA-Serpent mRNA in P. vivax-infected A. aquasalis and functional RNAi experiments identified this transcription factor as an important immune gene of A. aquasalis against both bacteria and P. vivax. Besides, we were able to identify an effect of GATA-Serpent knockdown on A. aquasalis hemocyte proliferation and differentiation. These findings expand our understanding of the poorly studied A. aquasalis-P. vivax interactions and uncover GATA-Serpent as a key player of the mosquito innate immune response.Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Laboratório de Biologia Molecular de Parasitas e Vetores Instituto Oswaldo Cruz FiocruzCentro de Desenvolvimento Tecnológico em Saúde (CDTS) Instituto Oswaldo CruzLaboratório de Fisiologia de Insetos Hematófagos Instituto de Biologia Universidade Federal de Minas GeraisLaboratório de Bioquímica de Artrópodes Hematófagos Instituto de Bioquímica Médica Leopoldo De Meis Universidade Federal do Rio de JaneiroLaboratório de Entomologia Médica Instituto René Rachou FiocruzLaboratório de Ecologia de Doenças Transmissíveis na Amazônia Instituto Leônidas & Maria Deane FiocruzFundação de Medicina Tropical Dr. Heitor Vieira DouradoLaboratório de Biodiversidade em Saúde Centro de Pesquisa Leônidas & Maria Deane FiocruzLaboratório de Bioquímica de Parasitos e Vetores Instituto de Biofísica Carlos Chagas FilhoSão Paulo State University (UNESP) School of Agricultural Sciences Department of Bioprocesses and BiotechnologySão Paulo State University (UNESP) School of Agricultural Sciences Department of Bioprocesses and BiotechnologyFiocruzInstituto Oswaldo CruzUniversidade Federal de Minas Gerais (UFMG)Universidade Federal do Rio de Janeiro (UFRJ)Fundação de Medicina Tropical Dr. Heitor Vieira DouradoInstituto de Biofísica Carlos Chagas FilhoUniversidade Estadual Paulista (Unesp)Bahia, Ana C.Kubota, Marina S.Souza-Neto, Jayme A. [UNESP]Koerich, Leonardo B.Barletta, Ana BeatrizAraújo, Helena R. C.Gonçalves, Caroline M.Ríos-Velásquez, Cláudia M.Pimenta, Paulo F. P.Traub-Csekö, Yara M.2019-10-06T15:20:08Z2019-10-06T15:20:08Z2018-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pntd.0006785PLoS Neglected Tropical Diseases, v. 12, n. 9, 2018.1935-27351935-2727http://hdl.handle.net/11449/18692810.1371/journal.pntd.00067852-s2.0-85054467562Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS Neglected Tropical Diseasesinfo:eu-repo/semantics/openAccess2021-10-23T11:59:56Zoai:repositorio.unesp.br:11449/186928Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T11:59:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
An Anopheles aquasalis GATA factor Serpent is required for immunity against Plasmodium and bacteria |
title |
An Anopheles aquasalis GATA factor Serpent is required for immunity against Plasmodium and bacteria |
spellingShingle |
An Anopheles aquasalis GATA factor Serpent is required for immunity against Plasmodium and bacteria Bahia, Ana C. |
title_short |
An Anopheles aquasalis GATA factor Serpent is required for immunity against Plasmodium and bacteria |
title_full |
An Anopheles aquasalis GATA factor Serpent is required for immunity against Plasmodium and bacteria |
title_fullStr |
An Anopheles aquasalis GATA factor Serpent is required for immunity against Plasmodium and bacteria |
title_full_unstemmed |
An Anopheles aquasalis GATA factor Serpent is required for immunity against Plasmodium and bacteria |
title_sort |
An Anopheles aquasalis GATA factor Serpent is required for immunity against Plasmodium and bacteria |
author |
Bahia, Ana C. |
author_facet |
Bahia, Ana C. Kubota, Marina S. Souza-Neto, Jayme A. [UNESP] Koerich, Leonardo B. Barletta, Ana Beatriz Araújo, Helena R. C. Gonçalves, Caroline M. Ríos-Velásquez, Cláudia M. Pimenta, Paulo F. P. Traub-Csekö, Yara M. |
author_role |
author |
author2 |
Kubota, Marina S. Souza-Neto, Jayme A. [UNESP] Koerich, Leonardo B. Barletta, Ana Beatriz Araújo, Helena R. C. Gonçalves, Caroline M. Ríos-Velásquez, Cláudia M. Pimenta, Paulo F. P. Traub-Csekö, Yara M. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Fiocruz Instituto Oswaldo Cruz Universidade Federal de Minas Gerais (UFMG) Universidade Federal do Rio de Janeiro (UFRJ) Fundação de Medicina Tropical Dr. Heitor Vieira Dourado Instituto de Biofísica Carlos Chagas Filho Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bahia, Ana C. Kubota, Marina S. Souza-Neto, Jayme A. [UNESP] Koerich, Leonardo B. Barletta, Ana Beatriz Araújo, Helena R. C. Gonçalves, Caroline M. Ríos-Velásquez, Cláudia M. Pimenta, Paulo F. P. Traub-Csekö, Yara M. |
description |
Innate immunity is an ancient and conserved defense system that provides an early effective response against invaders. Many immune genes of Anopheles mosquitoes have been implicated in defense against a variety of pathogens, including plasmodia. Nevertheless, only recent work identified some immune genes of Anopheles aquasalis mosquitoes upon P. vivax infection. Among these was a GATA transcription factor gene, which is described here. This is an ortholog of GATA factor Serpent genes described in Drosophila melanogaster and Anopheles gambiae. Gene expression analyses showed an increase of GATA-Serpent mRNA in P. vivax-infected A. aquasalis and functional RNAi experiments identified this transcription factor as an important immune gene of A. aquasalis against both bacteria and P. vivax. Besides, we were able to identify an effect of GATA-Serpent knockdown on A. aquasalis hemocyte proliferation and differentiation. These findings expand our understanding of the poorly studied A. aquasalis-P. vivax interactions and uncover GATA-Serpent as a key player of the mosquito innate immune response. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-09-01 2019-10-06T15:20:08Z 2019-10-06T15:20:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pntd.0006785 PLoS Neglected Tropical Diseases, v. 12, n. 9, 2018. 1935-2735 1935-2727 http://hdl.handle.net/11449/186928 10.1371/journal.pntd.0006785 2-s2.0-85054467562 |
url |
http://dx.doi.org/10.1371/journal.pntd.0006785 http://hdl.handle.net/11449/186928 |
identifier_str_mv |
PLoS Neglected Tropical Diseases, v. 12, n. 9, 2018. 1935-2735 1935-2727 10.1371/journal.pntd.0006785 2-s2.0-85054467562 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS Neglected Tropical Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965164449038336 |