Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1746-6148-9-83 http://hdl.handle.net/11449/75140 |
Resumo: | Background: The antibody Ki-67 is a reliable and easy tool to accurately assess the growth fraction of neoplasms in humans and animals, and it has been used to predict the clinical outcome. Therefore, the aim of the present study was to investigate the immunohistochemical expression pattern of Ki-67 in normal and neoplastic perianal glands of dogs to evaluate the possible use of this proliferation marker as an ancillary method of perianal tumor diagnosis. We studied 42 cases of perianal gland neoplasms including adenomas (n = 15), epitheliomas (n = 15), and carcinomas (n = 12). As controls, 13 tissue samples from normal perianal glands were used. A Ki-67 index was established by a computer-assisted image analysis and compared with manual counting. Results: Out of the 42 cases of perianal gland neoplasms, 34 were from males and eight from females. Recurrence was reported in 14 cases, being higher (8/12) in carcinomas. Immunostaining for Ki-67 revealed that the carcinomas showed a higher proliferation rate (9.87%) compared to groups of epitheliomas (2.66%) and adenomas (0.36%). For adenomas and epitheliomas of the perianal glands the computer-assisted counting and the manual counting gave similar results; however, only the computer-assisted image analysis was efficient to predict the perianal gland carcinoma recurrence.Conclusion: Since there were significant differences in the number of Ki-67-positive nuclei, this marker proved to be effective in helping the classification of perianal gland neoplasms and to refine the diagnosis criteria, especially in those samples with high variation in morphology/area. Also, higher Ki-67 index is related to recurrence in cases of perianal gland carcinomas. Further, the computer-assisted image analysis proved to be a fast and reliable method to assess the Ki-67 index in perianal gland neoplasms. © 2013 Pereira et al.; licensee BioMed Central Ltd. |
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Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognosticCell proliferationComputer-assisted image analysisDogsImmunohistochemistryMIB-1AnimaliaCanis familiarisBackground: The antibody Ki-67 is a reliable and easy tool to accurately assess the growth fraction of neoplasms in humans and animals, and it has been used to predict the clinical outcome. Therefore, the aim of the present study was to investigate the immunohistochemical expression pattern of Ki-67 in normal and neoplastic perianal glands of dogs to evaluate the possible use of this proliferation marker as an ancillary method of perianal tumor diagnosis. We studied 42 cases of perianal gland neoplasms including adenomas (n = 15), epitheliomas (n = 15), and carcinomas (n = 12). As controls, 13 tissue samples from normal perianal glands were used. A Ki-67 index was established by a computer-assisted image analysis and compared with manual counting. Results: Out of the 42 cases of perianal gland neoplasms, 34 were from males and eight from females. Recurrence was reported in 14 cases, being higher (8/12) in carcinomas. Immunostaining for Ki-67 revealed that the carcinomas showed a higher proliferation rate (9.87%) compared to groups of epitheliomas (2.66%) and adenomas (0.36%). For adenomas and epitheliomas of the perianal glands the computer-assisted counting and the manual counting gave similar results; however, only the computer-assisted image analysis was efficient to predict the perianal gland carcinoma recurrence.Conclusion: Since there were significant differences in the number of Ki-67-positive nuclei, this marker proved to be effective in helping the classification of perianal gland neoplasms and to refine the diagnosis criteria, especially in those samples with high variation in morphology/area. Also, higher Ki-67 index is related to recurrence in cases of perianal gland carcinomas. Further, the computer-assisted image analysis proved to be a fast and reliable method to assess the Ki-67 index in perianal gland neoplasms. © 2013 Pereira et al.; licensee BioMed Central Ltd.Universidade Estadual Paulista (UNESP) College of Veterinary Medicine Araçatuba, São PauloSchool of Veterinary Medicine Centro Universitário de Rio Preto (UNIRP) São José do Rio Preto, São PauloVETPAT - Laboratório de Patologia e Biologia Molecular Veterinária Campinas, São PauloDepartamento de Clínica, Cirurgia e Reprodução Animal Faculdade de Medicina Veterinária UNESP - Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Clóvis Pestana, 793, Araçatuba, SP CEP 16050-680Universidade Estadual Paulista (UNESP) College of Veterinary Medicine Araçatuba, São PauloDepartamento de Clínica, Cirurgia e Reprodução Animal Faculdade de Medicina Veterinária UNESP - Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Clóvis Pestana, 793, Araçatuba, SP CEP 16050-680Universidade Estadual Paulista (Unesp)São José do Rio PretoVETPAT - Laboratório de Patologia e Biologia Molecular Veterinária CampinasPereira, Rodrigo Storti [UNESP]Schweigert, Augusto [UNESP]Melo, Guilherme Dias de [UNESP]Fernandes, Fernando Vissani [UNESP]Sueiro, Felipe Augusto RuizMachado, Gisele Fabrino [UNESP]2014-05-27T11:28:56Z2014-05-27T11:28:56Z2013-04-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/1746-6148-9-83BMC Veterinary Research, v. 9.1746-6148http://hdl.handle.net/11449/7514010.1186/1746-6148-9-83WOS:0003186601000012-s2.0-848769539792-s2.0-84876953979.pdf0310405558125634Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Veterinary Research1.9580,934info:eu-repo/semantics/openAccess2024-09-04T18:03:30Zoai:repositorio.unesp.br:11449/75140Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:03:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic |
title |
Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic |
spellingShingle |
Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic Pereira, Rodrigo Storti [UNESP] Cell proliferation Computer-assisted image analysis Dogs Immunohistochemistry MIB-1 Animalia Canis familiaris |
title_short |
Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic |
title_full |
Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic |
title_fullStr |
Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic |
title_full_unstemmed |
Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic |
title_sort |
Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic |
author |
Pereira, Rodrigo Storti [UNESP] |
author_facet |
Pereira, Rodrigo Storti [UNESP] Schweigert, Augusto [UNESP] Melo, Guilherme Dias de [UNESP] Fernandes, Fernando Vissani [UNESP] Sueiro, Felipe Augusto Ruiz Machado, Gisele Fabrino [UNESP] |
author_role |
author |
author2 |
Schweigert, Augusto [UNESP] Melo, Guilherme Dias de [UNESP] Fernandes, Fernando Vissani [UNESP] Sueiro, Felipe Augusto Ruiz Machado, Gisele Fabrino [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) São José do Rio Preto VETPAT - Laboratório de Patologia e Biologia Molecular Veterinária Campinas |
dc.contributor.author.fl_str_mv |
Pereira, Rodrigo Storti [UNESP] Schweigert, Augusto [UNESP] Melo, Guilherme Dias de [UNESP] Fernandes, Fernando Vissani [UNESP] Sueiro, Felipe Augusto Ruiz Machado, Gisele Fabrino [UNESP] |
dc.subject.por.fl_str_mv |
Cell proliferation Computer-assisted image analysis Dogs Immunohistochemistry MIB-1 Animalia Canis familiaris |
topic |
Cell proliferation Computer-assisted image analysis Dogs Immunohistochemistry MIB-1 Animalia Canis familiaris |
description |
Background: The antibody Ki-67 is a reliable and easy tool to accurately assess the growth fraction of neoplasms in humans and animals, and it has been used to predict the clinical outcome. Therefore, the aim of the present study was to investigate the immunohistochemical expression pattern of Ki-67 in normal and neoplastic perianal glands of dogs to evaluate the possible use of this proliferation marker as an ancillary method of perianal tumor diagnosis. We studied 42 cases of perianal gland neoplasms including adenomas (n = 15), epitheliomas (n = 15), and carcinomas (n = 12). As controls, 13 tissue samples from normal perianal glands were used. A Ki-67 index was established by a computer-assisted image analysis and compared with manual counting. Results: Out of the 42 cases of perianal gland neoplasms, 34 were from males and eight from females. Recurrence was reported in 14 cases, being higher (8/12) in carcinomas. Immunostaining for Ki-67 revealed that the carcinomas showed a higher proliferation rate (9.87%) compared to groups of epitheliomas (2.66%) and adenomas (0.36%). For adenomas and epitheliomas of the perianal glands the computer-assisted counting and the manual counting gave similar results; however, only the computer-assisted image analysis was efficient to predict the perianal gland carcinoma recurrence.Conclusion: Since there were significant differences in the number of Ki-67-positive nuclei, this marker proved to be effective in helping the classification of perianal gland neoplasms and to refine the diagnosis criteria, especially in those samples with high variation in morphology/area. Also, higher Ki-67 index is related to recurrence in cases of perianal gland carcinomas. Further, the computer-assisted image analysis proved to be a fast and reliable method to assess the Ki-67 index in perianal gland neoplasms. © 2013 Pereira et al.; licensee BioMed Central Ltd. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04-20 2014-05-27T11:28:56Z 2014-05-27T11:28:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1746-6148-9-83 BMC Veterinary Research, v. 9. 1746-6148 http://hdl.handle.net/11449/75140 10.1186/1746-6148-9-83 WOS:000318660100001 2-s2.0-84876953979 2-s2.0-84876953979.pdf 0310405558125634 |
url |
http://dx.doi.org/10.1186/1746-6148-9-83 http://hdl.handle.net/11449/75140 |
identifier_str_mv |
BMC Veterinary Research, v. 9. 1746-6148 10.1186/1746-6148-9-83 WOS:000318660100001 2-s2.0-84876953979 2-s2.0-84876953979.pdf 0310405558125634 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Veterinary Research 1.958 0,934 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021366465822720 |