Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic

Detalhes bibliográficos
Autor(a) principal: Pereira, Rodrigo Storti [UNESP]
Data de Publicação: 2013
Outros Autores: Schweigert, Augusto [UNESP], Melo, Guilherme Dias de [UNESP], Fernandes, Fernando Vissani [UNESP], Sueiro, Felipe Augusto Ruiz, Machado, Gisele Fabrino [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/1746-6148-9-83
http://hdl.handle.net/11449/75140
Resumo: Background: The antibody Ki-67 is a reliable and easy tool to accurately assess the growth fraction of neoplasms in humans and animals, and it has been used to predict the clinical outcome. Therefore, the aim of the present study was to investigate the immunohistochemical expression pattern of Ki-67 in normal and neoplastic perianal glands of dogs to evaluate the possible use of this proliferation marker as an ancillary method of perianal tumor diagnosis. We studied 42 cases of perianal gland neoplasms including adenomas (n = 15), epitheliomas (n = 15), and carcinomas (n = 12). As controls, 13 tissue samples from normal perianal glands were used. A Ki-67 index was established by a computer-assisted image analysis and compared with manual counting. Results: Out of the 42 cases of perianal gland neoplasms, 34 were from males and eight from females. Recurrence was reported in 14 cases, being higher (8/12) in carcinomas. Immunostaining for Ki-67 revealed that the carcinomas showed a higher proliferation rate (9.87%) compared to groups of epitheliomas (2.66%) and adenomas (0.36%). For adenomas and epitheliomas of the perianal glands the computer-assisted counting and the manual counting gave similar results; however, only the computer-assisted image analysis was efficient to predict the perianal gland carcinoma recurrence.Conclusion: Since there were significant differences in the number of Ki-67-positive nuclei, this marker proved to be effective in helping the classification of perianal gland neoplasms and to refine the diagnosis criteria, especially in those samples with high variation in morphology/area. Also, higher Ki-67 index is related to recurrence in cases of perianal gland carcinomas. Further, the computer-assisted image analysis proved to be a fast and reliable method to assess the Ki-67 index in perianal gland neoplasms. © 2013 Pereira et al.; licensee BioMed Central Ltd.
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spelling Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognosticCell proliferationComputer-assisted image analysisDogsImmunohistochemistryMIB-1AnimaliaCanis familiarisBackground: The antibody Ki-67 is a reliable and easy tool to accurately assess the growth fraction of neoplasms in humans and animals, and it has been used to predict the clinical outcome. Therefore, the aim of the present study was to investigate the immunohistochemical expression pattern of Ki-67 in normal and neoplastic perianal glands of dogs to evaluate the possible use of this proliferation marker as an ancillary method of perianal tumor diagnosis. We studied 42 cases of perianal gland neoplasms including adenomas (n = 15), epitheliomas (n = 15), and carcinomas (n = 12). As controls, 13 tissue samples from normal perianal glands were used. A Ki-67 index was established by a computer-assisted image analysis and compared with manual counting. Results: Out of the 42 cases of perianal gland neoplasms, 34 were from males and eight from females. Recurrence was reported in 14 cases, being higher (8/12) in carcinomas. Immunostaining for Ki-67 revealed that the carcinomas showed a higher proliferation rate (9.87%) compared to groups of epitheliomas (2.66%) and adenomas (0.36%). For adenomas and epitheliomas of the perianal glands the computer-assisted counting and the manual counting gave similar results; however, only the computer-assisted image analysis was efficient to predict the perianal gland carcinoma recurrence.Conclusion: Since there were significant differences in the number of Ki-67-positive nuclei, this marker proved to be effective in helping the classification of perianal gland neoplasms and to refine the diagnosis criteria, especially in those samples with high variation in morphology/area. Also, higher Ki-67 index is related to recurrence in cases of perianal gland carcinomas. Further, the computer-assisted image analysis proved to be a fast and reliable method to assess the Ki-67 index in perianal gland neoplasms. © 2013 Pereira et al.; licensee BioMed Central Ltd.Universidade Estadual Paulista (UNESP) College of Veterinary Medicine Araçatuba, São PauloSchool of Veterinary Medicine Centro Universitário de Rio Preto (UNIRP) São José do Rio Preto, São PauloVETPAT - Laboratório de Patologia e Biologia Molecular Veterinária Campinas, São PauloDepartamento de Clínica, Cirurgia e Reprodução Animal Faculdade de Medicina Veterinária UNESP - Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Clóvis Pestana, 793, Araçatuba, SP CEP 16050-680Universidade Estadual Paulista (UNESP) College of Veterinary Medicine Araçatuba, São PauloDepartamento de Clínica, Cirurgia e Reprodução Animal Faculdade de Medicina Veterinária UNESP - Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Clóvis Pestana, 793, Araçatuba, SP CEP 16050-680Universidade Estadual Paulista (Unesp)São José do Rio PretoVETPAT - Laboratório de Patologia e Biologia Molecular Veterinária CampinasPereira, Rodrigo Storti [UNESP]Schweigert, Augusto [UNESP]Melo, Guilherme Dias de [UNESP]Fernandes, Fernando Vissani [UNESP]Sueiro, Felipe Augusto RuizMachado, Gisele Fabrino [UNESP]2014-05-27T11:28:56Z2014-05-27T11:28:56Z2013-04-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/1746-6148-9-83BMC Veterinary Research, v. 9.1746-6148http://hdl.handle.net/11449/7514010.1186/1746-6148-9-83WOS:0003186601000012-s2.0-848769539792-s2.0-84876953979.pdf0310405558125634Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Veterinary Research1.9580,934info:eu-repo/semantics/openAccess2024-09-04T18:03:30Zoai:repositorio.unesp.br:11449/75140Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:03:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic
title Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic
spellingShingle Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic
Pereira, Rodrigo Storti [UNESP]
Cell proliferation
Computer-assisted image analysis
Dogs
Immunohistochemistry
MIB-1
Animalia
Canis familiaris
title_short Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic
title_full Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic
title_fullStr Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic
title_full_unstemmed Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic
title_sort Ki-67 labeling in canine perianal glands neoplasms: A novel approach for immunohistological diagnostic and prognostic
author Pereira, Rodrigo Storti [UNESP]
author_facet Pereira, Rodrigo Storti [UNESP]
Schweigert, Augusto [UNESP]
Melo, Guilherme Dias de [UNESP]
Fernandes, Fernando Vissani [UNESP]
Sueiro, Felipe Augusto Ruiz
Machado, Gisele Fabrino [UNESP]
author_role author
author2 Schweigert, Augusto [UNESP]
Melo, Guilherme Dias de [UNESP]
Fernandes, Fernando Vissani [UNESP]
Sueiro, Felipe Augusto Ruiz
Machado, Gisele Fabrino [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
São José do Rio Preto
VETPAT - Laboratório de Patologia e Biologia Molecular Veterinária Campinas
dc.contributor.author.fl_str_mv Pereira, Rodrigo Storti [UNESP]
Schweigert, Augusto [UNESP]
Melo, Guilherme Dias de [UNESP]
Fernandes, Fernando Vissani [UNESP]
Sueiro, Felipe Augusto Ruiz
Machado, Gisele Fabrino [UNESP]
dc.subject.por.fl_str_mv Cell proliferation
Computer-assisted image analysis
Dogs
Immunohistochemistry
MIB-1
Animalia
Canis familiaris
topic Cell proliferation
Computer-assisted image analysis
Dogs
Immunohistochemistry
MIB-1
Animalia
Canis familiaris
description Background: The antibody Ki-67 is a reliable and easy tool to accurately assess the growth fraction of neoplasms in humans and animals, and it has been used to predict the clinical outcome. Therefore, the aim of the present study was to investigate the immunohistochemical expression pattern of Ki-67 in normal and neoplastic perianal glands of dogs to evaluate the possible use of this proliferation marker as an ancillary method of perianal tumor diagnosis. We studied 42 cases of perianal gland neoplasms including adenomas (n = 15), epitheliomas (n = 15), and carcinomas (n = 12). As controls, 13 tissue samples from normal perianal glands were used. A Ki-67 index was established by a computer-assisted image analysis and compared with manual counting. Results: Out of the 42 cases of perianal gland neoplasms, 34 were from males and eight from females. Recurrence was reported in 14 cases, being higher (8/12) in carcinomas. Immunostaining for Ki-67 revealed that the carcinomas showed a higher proliferation rate (9.87%) compared to groups of epitheliomas (2.66%) and adenomas (0.36%). For adenomas and epitheliomas of the perianal glands the computer-assisted counting and the manual counting gave similar results; however, only the computer-assisted image analysis was efficient to predict the perianal gland carcinoma recurrence.Conclusion: Since there were significant differences in the number of Ki-67-positive nuclei, this marker proved to be effective in helping the classification of perianal gland neoplasms and to refine the diagnosis criteria, especially in those samples with high variation in morphology/area. Also, higher Ki-67 index is related to recurrence in cases of perianal gland carcinomas. Further, the computer-assisted image analysis proved to be a fast and reliable method to assess the Ki-67 index in perianal gland neoplasms. © 2013 Pereira et al.; licensee BioMed Central Ltd.
publishDate 2013
dc.date.none.fl_str_mv 2013-04-20
2014-05-27T11:28:56Z
2014-05-27T11:28:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/1746-6148-9-83
BMC Veterinary Research, v. 9.
1746-6148
http://hdl.handle.net/11449/75140
10.1186/1746-6148-9-83
WOS:000318660100001
2-s2.0-84876953979
2-s2.0-84876953979.pdf
0310405558125634
url http://dx.doi.org/10.1186/1746-6148-9-83
http://hdl.handle.net/11449/75140
identifier_str_mv BMC Veterinary Research, v. 9.
1746-6148
10.1186/1746-6148-9-83
WOS:000318660100001
2-s2.0-84876953979
2-s2.0-84876953979.pdf
0310405558125634
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv BMC Veterinary Research
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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