Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/226097 |
Resumo: | Previously, we have demonstrated that treatment of experimental diabetes with a decoction of Bauhinia forficata leaves is beneficial. In this study, we prepared a two-fold concentrate of this extract and tested its effects on physiological, biochemical and toxicity markers in streptozotocin-diabetic rats. Dried and ground leaves were extracted with warm 70% hydroethanol and the filtrate concentrated by evaporation at 50°C. This solution was mixed with colloidal silicon dioxide (Tixosil-333®) and dried in a spouted bed (BfT). Rats were treated with water, insulin and Tixosil particles at low or high doses, alone or coated with dried BfT. Animals were periodically weighed and monitored for water and food intake; urinary volume, glucose, urea and protein; blood glucose, serum lipids, liver toxicity markers transaminase and phosphatase and masses of adipose tissue and skeletal muscle. Insulin treatment gave best rat growth and lowest values for all other markers. No other treatment affected any diabetic marker, but the enzyme activities were changed by diabetes and BfT. Thus, BfT toxicity could arise from secondary products of plant constituents or Tixosil interaction. Therefore, BfT prepared in the spouted bed as described, is unsuitable for treatment of diabetes, which implies that the method of preparation of any medicine is critical for its efficacy and toxicity. © 2010 Academic Journals. |
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Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in ratsBauhinia forficataEnzymesPhysiological variablesPlasma glucoseSerum lipidsSerum toxicity markersSpouted bed dryingPreviously, we have demonstrated that treatment of experimental diabetes with a decoction of Bauhinia forficata leaves is beneficial. In this study, we prepared a two-fold concentrate of this extract and tested its effects on physiological, biochemical and toxicity markers in streptozotocin-diabetic rats. Dried and ground leaves were extracted with warm 70% hydroethanol and the filtrate concentrated by evaporation at 50°C. This solution was mixed with colloidal silicon dioxide (Tixosil-333®) and dried in a spouted bed (BfT). Rats were treated with water, insulin and Tixosil particles at low or high doses, alone or coated with dried BfT. Animals were periodically weighed and monitored for water and food intake; urinary volume, glucose, urea and protein; blood glucose, serum lipids, liver toxicity markers transaminase and phosphatase and masses of adipose tissue and skeletal muscle. Insulin treatment gave best rat growth and lowest values for all other markers. No other treatment affected any diabetic marker, but the enzyme activities were changed by diabetes and BfT. Thus, BfT toxicity could arise from secondary products of plant constituents or Tixosil interaction. Therefore, BfT prepared in the spouted bed as described, is unsuitable for treatment of diabetes, which implies that the method of preparation of any medicine is critical for its efficacy and toxicity. © 2010 Academic Journals.Departamento de Análises Clínicas Faculdade de Ciências Farmacêuticas, UNESP Universidade Estadual Paulista, São PauloLaboratório de Processos Farmacêuticos Faculdade de Ciências Farmacêuticas de Ribeirão Preto, USP Universidade de São PauloDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas, UNESP Universidade Estadual Paulista, São PauloUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Pepato, M. T. [UNESP]Conceição, C. Q. [UNESP]Gutierres, V. O. [UNESP]Vendramini, R. C. [UNESP]Souza, C. R.F.Oliveira, W. P.Brunetti, I. L. [UNESP]2022-04-28T21:25:15Z2022-04-28T21:25:15Z2010-10-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7165-7173African Journal of Biotechnology, v. 9, n. 42, p. 7165-7173, 2010.1684-5315http://hdl.handle.net/11449/2260972-s2.0-78049473493Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAfrican Journal of Biotechnologyinfo:eu-repo/semantics/openAccess2022-04-28T21:25:15Zoai:repositorio.unesp.br:11449/226097Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T21:25:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats |
title |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats |
spellingShingle |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats Pepato, M. T. [UNESP] Bauhinia forficata Enzymes Physiological variables Plasma glucose Serum lipids Serum toxicity markers Spouted bed drying |
title_short |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats |
title_full |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats |
title_fullStr |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats |
title_full_unstemmed |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats |
title_sort |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats |
author |
Pepato, M. T. [UNESP] |
author_facet |
Pepato, M. T. [UNESP] Conceição, C. Q. [UNESP] Gutierres, V. O. [UNESP] Vendramini, R. C. [UNESP] Souza, C. R.F. Oliveira, W. P. Brunetti, I. L. [UNESP] |
author_role |
author |
author2 |
Conceição, C. Q. [UNESP] Gutierres, V. O. [UNESP] Vendramini, R. C. [UNESP] Souza, C. R.F. Oliveira, W. P. Brunetti, I. L. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Pepato, M. T. [UNESP] Conceição, C. Q. [UNESP] Gutierres, V. O. [UNESP] Vendramini, R. C. [UNESP] Souza, C. R.F. Oliveira, W. P. Brunetti, I. L. [UNESP] |
dc.subject.por.fl_str_mv |
Bauhinia forficata Enzymes Physiological variables Plasma glucose Serum lipids Serum toxicity markers Spouted bed drying |
topic |
Bauhinia forficata Enzymes Physiological variables Plasma glucose Serum lipids Serum toxicity markers Spouted bed drying |
description |
Previously, we have demonstrated that treatment of experimental diabetes with a decoction of Bauhinia forficata leaves is beneficial. In this study, we prepared a two-fold concentrate of this extract and tested its effects on physiological, biochemical and toxicity markers in streptozotocin-diabetic rats. Dried and ground leaves were extracted with warm 70% hydroethanol and the filtrate concentrated by evaporation at 50°C. This solution was mixed with colloidal silicon dioxide (Tixosil-333®) and dried in a spouted bed (BfT). Rats were treated with water, insulin and Tixosil particles at low or high doses, alone or coated with dried BfT. Animals were periodically weighed and monitored for water and food intake; urinary volume, glucose, urea and protein; blood glucose, serum lipids, liver toxicity markers transaminase and phosphatase and masses of adipose tissue and skeletal muscle. Insulin treatment gave best rat growth and lowest values for all other markers. No other treatment affected any diabetic marker, but the enzyme activities were changed by diabetes and BfT. Thus, BfT toxicity could arise from secondary products of plant constituents or Tixosil interaction. Therefore, BfT prepared in the spouted bed as described, is unsuitable for treatment of diabetes, which implies that the method of preparation of any medicine is critical for its efficacy and toxicity. © 2010 Academic Journals. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10-18 2022-04-28T21:25:15Z 2022-04-28T21:25:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
African Journal of Biotechnology, v. 9, n. 42, p. 7165-7173, 2010. 1684-5315 http://hdl.handle.net/11449/226097 2-s2.0-78049473493 |
identifier_str_mv |
African Journal of Biotechnology, v. 9, n. 42, p. 7165-7173, 2010. 1684-5315 2-s2.0-78049473493 |
url |
http://hdl.handle.net/11449/226097 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
African Journal of Biotechnology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7165-7173 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965522276646912 |