Ehrlichia sp infection in carthorses of low-income owners, Southern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.cimid.2016.07.002 http://hdl.handle.net/11449/161966 |
Resumo: | Although well established in dogs, Ehrlichia sp. infection has been scarcely reported in horses. The aim was to perform a comprehensive serological and molecular survey for the detection of Ehrlichia spp. in carthorses from Southern Brazil. Blood samples from 190 carthorses from Parana State were sampled. Horses were also tested for Borrelia burgdorferi and Anaplasma phagocytophilum. Anti-Ehrlichia sp. antibodies were detected by a commercial rapid ELISA, and immunofluorescence antibody assays (IFA) with E. chaffeensis and E. canis as crude antigens. The molecular and phylogenetic analysis of Ehrlichia sp. was based on 16 S rRNA and dsb genes. A total of 52 (27.4%), 4 (2.1%), and 3 (1.6%) horses were positive for Ehrlichia spp., Anaplasma spp. and Borrelia burgdorferi, respectively, by the commercial rapid ELISA. Thirty-eight (20.0%) and 37 (19.5%) horses showed anti-E. chaffeensis and anti-E. canis antibodies by IFA, respectively. One blood sample that also showed anti-E. chaffeensis antibodies was PCR positive for the 16 S rRNA and dsb genes of Ehrlichia spp., showing an identity of >98.0% to the uncultured Ehrlichia sp. previously detected in Brazilian jaguars (Panthera onca). Anti-Ehrlichia sp. antibodies and Ehrlichia DNA were detected in carthorses from Southern Brazil, which may post public health concerns due to intimate contact with low-income owners. This is the first report of a natural infection of this bacteria in horses from South America. Clinical signs and the tick vector remain unknown. (C) 2016 Published by Elsevier Ltd. |
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Ehrlichia sp infection in carthorses of low-income owners, Southern BrazilVector-borne diseaseTick-borne diseaseEhrlichiosisAlthough well established in dogs, Ehrlichia sp. infection has been scarcely reported in horses. The aim was to perform a comprehensive serological and molecular survey for the detection of Ehrlichia spp. in carthorses from Southern Brazil. Blood samples from 190 carthorses from Parana State were sampled. Horses were also tested for Borrelia burgdorferi and Anaplasma phagocytophilum. Anti-Ehrlichia sp. antibodies were detected by a commercial rapid ELISA, and immunofluorescence antibody assays (IFA) with E. chaffeensis and E. canis as crude antigens. The molecular and phylogenetic analysis of Ehrlichia sp. was based on 16 S rRNA and dsb genes. A total of 52 (27.4%), 4 (2.1%), and 3 (1.6%) horses were positive for Ehrlichia spp., Anaplasma spp. and Borrelia burgdorferi, respectively, by the commercial rapid ELISA. Thirty-eight (20.0%) and 37 (19.5%) horses showed anti-E. chaffeensis and anti-E. canis antibodies by IFA, respectively. One blood sample that also showed anti-E. chaffeensis antibodies was PCR positive for the 16 S rRNA and dsb genes of Ehrlichia spp., showing an identity of >98.0% to the uncultured Ehrlichia sp. previously detected in Brazilian jaguars (Panthera onca). Anti-Ehrlichia sp. antibodies and Ehrlichia DNA were detected in carthorses from Southern Brazil, which may post public health concerns due to intimate contact with low-income owners. This is the first report of a natural infection of this bacteria in horses from South America. Clinical signs and the tick vector remain unknown. (C) 2016 Published by Elsevier Ltd.Fundacao Araucaria do ParanaUniv Estadual Londrina, Coll Vet Med, Dept Vet Prevent Med, Rodovia Celso Garcia Cid,Pr 445, BR-86051990 Londrina, PR, BrazilUniv Fed Parana, Dept Vet Med, BR-80035050 Curitiba, PR, BrazilUniv Sao Paulo, Coll Vet Med, Dept Prevent Vet & Anim Hlth, BR-05508270 Sao Paulo, SP, BrazilSao Paulo State Univ, Coll Vet Med, Dept Clin Surg & Anim Reprod, BR-16050680 Aracatuba, SP, BrazilSao Paulo State Univ, Coll Vet Med, Dept Clin Surg & Anim Reprod, BR-16050680 Aracatuba, SP, BrazilElsevier B.V.Universidade Estadual de Londrina (UEL)Univ Fed ParanaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Vieira, Thallitha S.Vieira, Rafael F.Krawczak, Felipe S.Soares, Herbert S.Guimaraes, Ana M.Barros-Filho, Ivan R.Marcondes, Mary [UNESP]Labruna, Marcelo B.Biondo, Alexander W.Vidotto, Odilon2018-11-26T17:06:22Z2018-11-26T17:06:22Z2016-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-5application/pdfhttp://dx.doi.org/10.1016/j.cimid.2016.07.002Comparative Immunology Microbiology And Infectious Diseases. Oxford: Elsevier Sci Ltd, v. 48, p. 1-5, 2016.0147-9571http://hdl.handle.net/11449/16196610.1016/j.cimid.2016.07.002WOS:000384145900001WOS000384145900001.pdf1817946671090010Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Immunology Microbiology And Infectious Diseases0,877info:eu-repo/semantics/openAccess2024-09-04T18:03:58Zoai:repositorio.unesp.br:11449/161966Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:03:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ehrlichia sp infection in carthorses of low-income owners, Southern Brazil |
title |
Ehrlichia sp infection in carthorses of low-income owners, Southern Brazil |
spellingShingle |
Ehrlichia sp infection in carthorses of low-income owners, Southern Brazil Vieira, Thallitha S. Vector-borne disease Tick-borne disease Ehrlichiosis |
title_short |
Ehrlichia sp infection in carthorses of low-income owners, Southern Brazil |
title_full |
Ehrlichia sp infection in carthorses of low-income owners, Southern Brazil |
title_fullStr |
Ehrlichia sp infection in carthorses of low-income owners, Southern Brazil |
title_full_unstemmed |
Ehrlichia sp infection in carthorses of low-income owners, Southern Brazil |
title_sort |
Ehrlichia sp infection in carthorses of low-income owners, Southern Brazil |
author |
Vieira, Thallitha S. |
author_facet |
Vieira, Thallitha S. Vieira, Rafael F. Krawczak, Felipe S. Soares, Herbert S. Guimaraes, Ana M. Barros-Filho, Ivan R. Marcondes, Mary [UNESP] Labruna, Marcelo B. Biondo, Alexander W. Vidotto, Odilon |
author_role |
author |
author2 |
Vieira, Rafael F. Krawczak, Felipe S. Soares, Herbert S. Guimaraes, Ana M. Barros-Filho, Ivan R. Marcondes, Mary [UNESP] Labruna, Marcelo B. Biondo, Alexander W. Vidotto, Odilon |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) Univ Fed Parana Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Vieira, Thallitha S. Vieira, Rafael F. Krawczak, Felipe S. Soares, Herbert S. Guimaraes, Ana M. Barros-Filho, Ivan R. Marcondes, Mary [UNESP] Labruna, Marcelo B. Biondo, Alexander W. Vidotto, Odilon |
dc.subject.por.fl_str_mv |
Vector-borne disease Tick-borne disease Ehrlichiosis |
topic |
Vector-borne disease Tick-borne disease Ehrlichiosis |
description |
Although well established in dogs, Ehrlichia sp. infection has been scarcely reported in horses. The aim was to perform a comprehensive serological and molecular survey for the detection of Ehrlichia spp. in carthorses from Southern Brazil. Blood samples from 190 carthorses from Parana State were sampled. Horses were also tested for Borrelia burgdorferi and Anaplasma phagocytophilum. Anti-Ehrlichia sp. antibodies were detected by a commercial rapid ELISA, and immunofluorescence antibody assays (IFA) with E. chaffeensis and E. canis as crude antigens. The molecular and phylogenetic analysis of Ehrlichia sp. was based on 16 S rRNA and dsb genes. A total of 52 (27.4%), 4 (2.1%), and 3 (1.6%) horses were positive for Ehrlichia spp., Anaplasma spp. and Borrelia burgdorferi, respectively, by the commercial rapid ELISA. Thirty-eight (20.0%) and 37 (19.5%) horses showed anti-E. chaffeensis and anti-E. canis antibodies by IFA, respectively. One blood sample that also showed anti-E. chaffeensis antibodies was PCR positive for the 16 S rRNA and dsb genes of Ehrlichia spp., showing an identity of >98.0% to the uncultured Ehrlichia sp. previously detected in Brazilian jaguars (Panthera onca). Anti-Ehrlichia sp. antibodies and Ehrlichia DNA were detected in carthorses from Southern Brazil, which may post public health concerns due to intimate contact with low-income owners. This is the first report of a natural infection of this bacteria in horses from South America. Clinical signs and the tick vector remain unknown. (C) 2016 Published by Elsevier Ltd. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-01 2018-11-26T17:06:22Z 2018-11-26T17:06:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.cimid.2016.07.002 Comparative Immunology Microbiology And Infectious Diseases. Oxford: Elsevier Sci Ltd, v. 48, p. 1-5, 2016. 0147-9571 http://hdl.handle.net/11449/161966 10.1016/j.cimid.2016.07.002 WOS:000384145900001 WOS000384145900001.pdf 1817946671090010 |
url |
http://dx.doi.org/10.1016/j.cimid.2016.07.002 http://hdl.handle.net/11449/161966 |
identifier_str_mv |
Comparative Immunology Microbiology And Infectious Diseases. Oxford: Elsevier Sci Ltd, v. 48, p. 1-5, 2016. 0147-9571 10.1016/j.cimid.2016.07.002 WOS:000384145900001 WOS000384145900001.pdf 1817946671090010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comparative Immunology Microbiology And Infectious Diseases 0,877 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-5 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
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UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021395183173632 |