Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-017-11043-0 http://hdl.handle.net/11449/179149 |
Resumo: | This study presents an inexpensive and easy way to produce a microfluidic device that mimics a blood vessel, serving as a start point for cell culture under perfusion, cardiovascular research, and toxicological studies. Endpoint assays (i.e., MTT reduction and NO assays) were used and revealed that the components making up the microchip, which is made of polyester and toner (PT), did not induce cell death or nitric oxide (NO) production. Applying oxygen plasma and fibronectin improved the adhesion and proliferation endothelial cell along the microchannel. As expected, these treatments showed an increase in vascular endothelial growth factor (VEGF-A) concentration profiles, which is correlated with adherence and cell proliferation, thus promoting endothelialization of the device for neovascularization. Regardless the simplicity of the device, our vein-on-a-chip mimetic has a potential to serve as a powerful tool for those that demand a rapid microfabrication method in cell biology or organ-on-a-chip research. |
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Repositório Institucional da UNESP |
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Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic DeviceThis study presents an inexpensive and easy way to produce a microfluidic device that mimics a blood vessel, serving as a start point for cell culture under perfusion, cardiovascular research, and toxicological studies. Endpoint assays (i.e., MTT reduction and NO assays) were used and revealed that the components making up the microchip, which is made of polyester and toner (PT), did not induce cell death or nitric oxide (NO) production. Applying oxygen plasma and fibronectin improved the adhesion and proliferation endothelial cell along the microchannel. As expected, these treatments showed an increase in vascular endothelial growth factor (VEGF-A) concentration profiles, which is correlated with adherence and cell proliferation, thus promoting endothelialization of the device for neovascularization. Regardless the simplicity of the device, our vein-on-a-chip mimetic has a potential to serve as a powerful tool for those that demand a rapid microfabrication method in cell biology or organ-on-a-chip research.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Instituto de Química de São Carlos IQSC Universidade de São Paulo USPGHTM - Global Health and Tropical Medicine Instituto de Higiene e Medicina Tropical IHMT Universidade Nova de Lisboa UNLLaboratório de Parasitologia Departamento de Morfologia e Patologia Universidade Federal de São Carlos UFSCarEscola Paulista de Medicina Universidade Federal de São Paulo UnifespFaculdade de Ciências Farmacêuticas FCFar Universidade Estadual Paulista UNESPInstituto Nacional de Ciência e Tecnologia de Bioanalítica INCTBioFaculdade de Ciências Farmacêuticas FCFar Universidade Estadual Paulista UNESPCNPq: 150096/2014-3CNPq: 150250/2014-2CNPq: 311323/2011-1CNPq: 400168/2013-8CNPq: 482696/2011-7Universidade de São Paulo (USP)UNLUniversidade Federal de São Carlos (UFSCar)Universidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (Unesp)INCTBioUrbaczek, Ana CarolinaLeão, Paulo Augusto Gomes CarneiroDe Souza, Fayene Zeferino RibeiroAfonso, AnaVieira Alberice, JulianaCappelini, Luciana Teresa DiasCarlos, Iracilda Zeppone [UNESP]Carrilho, Emanuel2018-12-11T17:33:57Z2018-12-11T17:33:57Z2017-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1038/s41598-017-11043-0Scientific Reports, v. 7, n. 1, 2017.2045-2322http://hdl.handle.net/11449/17914910.1038/s41598-017-11043-02-s2.0-850288163222-s2.0-85028816322.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reports1,533info:eu-repo/semantics/openAccess2024-06-21T15:19:08Zoai:repositorio.unesp.br:11449/179149Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-21T15:19:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device |
title |
Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device |
spellingShingle |
Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device Urbaczek, Ana Carolina |
title_short |
Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device |
title_full |
Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device |
title_fullStr |
Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device |
title_full_unstemmed |
Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device |
title_sort |
Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device |
author |
Urbaczek, Ana Carolina |
author_facet |
Urbaczek, Ana Carolina Leão, Paulo Augusto Gomes Carneiro De Souza, Fayene Zeferino Ribeiro Afonso, Ana Vieira Alberice, Juliana Cappelini, Luciana Teresa Dias Carlos, Iracilda Zeppone [UNESP] Carrilho, Emanuel |
author_role |
author |
author2 |
Leão, Paulo Augusto Gomes Carneiro De Souza, Fayene Zeferino Ribeiro Afonso, Ana Vieira Alberice, Juliana Cappelini, Luciana Teresa Dias Carlos, Iracilda Zeppone [UNESP] Carrilho, Emanuel |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) UNL Universidade Federal de São Carlos (UFSCar) Universidade Federal de São Paulo (UNIFESP) Universidade Estadual Paulista (Unesp) INCTBio |
dc.contributor.author.fl_str_mv |
Urbaczek, Ana Carolina Leão, Paulo Augusto Gomes Carneiro De Souza, Fayene Zeferino Ribeiro Afonso, Ana Vieira Alberice, Juliana Cappelini, Luciana Teresa Dias Carlos, Iracilda Zeppone [UNESP] Carrilho, Emanuel |
description |
This study presents an inexpensive and easy way to produce a microfluidic device that mimics a blood vessel, serving as a start point for cell culture under perfusion, cardiovascular research, and toxicological studies. Endpoint assays (i.e., MTT reduction and NO assays) were used and revealed that the components making up the microchip, which is made of polyester and toner (PT), did not induce cell death or nitric oxide (NO) production. Applying oxygen plasma and fibronectin improved the adhesion and proliferation endothelial cell along the microchannel. As expected, these treatments showed an increase in vascular endothelial growth factor (VEGF-A) concentration profiles, which is correlated with adherence and cell proliferation, thus promoting endothelialization of the device for neovascularization. Regardless the simplicity of the device, our vein-on-a-chip mimetic has a potential to serve as a powerful tool for those that demand a rapid microfabrication method in cell biology or organ-on-a-chip research. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 2018-12-11T17:33:57Z 2018-12-11T17:33:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-017-11043-0 Scientific Reports, v. 7, n. 1, 2017. 2045-2322 http://hdl.handle.net/11449/179149 10.1038/s41598-017-11043-0 2-s2.0-85028816322 2-s2.0-85028816322.pdf |
url |
http://dx.doi.org/10.1038/s41598-017-11043-0 http://hdl.handle.net/11449/179149 |
identifier_str_mv |
Scientific Reports, v. 7, n. 1, 2017. 2045-2322 10.1038/s41598-017-11043-0 2-s2.0-85028816322 2-s2.0-85028816322.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports 1,533 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803649935335227392 |