Serological and molecular survey of Leptospira spp. among cart horses from an endemic area of human leptospirosis in Curitiba, southern Brazil

Detalhes bibliográficos
Autor(a) principal: Finger, Mariane Angelica
Data de Publicação: 2014
Outros Autores: Barros Filho, Ivan Roque de, Leutenegger, Christian, Estrada, Marko, Ullmann, Leila Sabrina [UNESP], Langoni, Helio [UNESP], Kikuti, Mariana [UNESP], Dornbush, Peterson Triches, Deconto, Ivan, Biondo, Alexander Welker
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652014000600473&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/129914
Resumo: Introduction: Cart horses are a re-emerging population employed to carry recyclable material in cities. Methods: Sixty-two horses were sampled in an endemic area of human leptospirosis. The microscopic agglutination test (MAT) and real-time polymerase chain reaction (qPCR) were performed. Results: A seropositivity of 75.8% with serovar Icterohaemorrhagiae in 80.8% of the horses was observed. Blood and urine were qPCR negative. MAT showed positive correlations with rainfall (p = 0.02) and flooding (p = 0.03). Conclusions: Although horses may be constantly exposed to Leptospira spp. in the environment mostly because of rainfall and flooding, no leptospiremia or leptospiruria were observed in this study.
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spelling Serological and molecular survey of Leptospira spp. among cart horses from an endemic area of human leptospirosis in Curitiba, southern BrazilLeptospira spp.MATqPCRHorsesIntroduction: Cart horses are a re-emerging population employed to carry recyclable material in cities. Methods: Sixty-two horses were sampled in an endemic area of human leptospirosis. The microscopic agglutination test (MAT) and real-time polymerase chain reaction (qPCR) were performed. Results: A seropositivity of 75.8% with serovar Icterohaemorrhagiae in 80.8% of the horses was observed. Blood and urine were qPCR negative. MAT showed positive correlations with rainfall (p = 0.02) and flooding (p = 0.03). Conclusions: Although horses may be constantly exposed to Leptospira spp. in the environment mostly because of rainfall and flooding, no leptospiremia or leptospiruria were observed in this study.Introdução: Cavalos carroceiros são uma população reemergente empregada para transportar materiais recicláveis em cidades. Métodos: Em área endêmica para leptospirose humana foram amostrados 62 cavalos. Soroaglutinação microscópica e reação em cadeia da polimerase em tempo real foram empregadas. Resultados: Observou-se soropositividade em 75,8% com sorovar Icterohaemorrhagiae em 80,8% cavalos. Amostras de sangue e urina foram negativas no qPCR. Observou-se correlação positiva entre SAM e pluviosidade (p = 0,02) e alagamentos (p = 0,03). Conclusão: Embora cavalos possam estar constantemente expostos a Leptospira spp. no ambiente, principalmente por chuvas e inundações, leptospiremia e leptospiruria não foram encontradas neste estudo.Real Time PCR Core Facility, Idexx Reference Laboratories. 2825 Kovr Dr, West Sacramento, CA, 95605, USADepartment of Veterinary Medicine, Federal University of Paraná (UFPR), 80035 Curitiba, PR, Brazil. Dr. Biondo is also a visiting professor at the Department of Pathobiology, University of Illinois, Urbana, IL, 61802, USA.Department of Veterinary Hygiene and Public Health, Veterinary School, São Paulo State University, 18618 Botucatu, SP, BrazilInst Medicina Tropical Sao PauloUniversidade Federal do Paraná (UFPR)University of IllinoisUniversidade Estadual Paulista (Unesp)Finger, Mariane AngelicaBarros Filho, Ivan Roque deLeutenegger, ChristianEstrada, MarkoUllmann, Leila Sabrina [UNESP]Langoni, Helio [UNESP]Kikuti, Mariana [UNESP]Dornbush, Peterson TrichesDeconto, IvanBiondo, Alexander Welker2015-11-03T15:27:45Z2015-11-03T15:27:45Z2014-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article473-476application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652014000600473&lng=en&nrm=iso&tlng=enRevista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo: Inst Medicina Tropical Sao Paulo, v. 56, n. 6, p. 473-476, 2014.0036-4665http://hdl.handle.net/11449/12991410.1590/S0036-46652014000600003S0036-46652014000600473WOS:000344333000003S0036-46652014000600473.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Do Instituto De Medicina Tropical De Sao Paulo1.4890,669info:eu-repo/semantics/openAccess2024-09-03T14:12:03Zoai:repositorio.unesp.br:11449/129914Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T14:12:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Serological and molecular survey of Leptospira spp. among cart horses from an endemic area of human leptospirosis in Curitiba, southern Brazil
title Serological and molecular survey of Leptospira spp. among cart horses from an endemic area of human leptospirosis in Curitiba, southern Brazil
spellingShingle Serological and molecular survey of Leptospira spp. among cart horses from an endemic area of human leptospirosis in Curitiba, southern Brazil
Finger, Mariane Angelica
Leptospira spp.
MAT
qPCR
Horses
title_short Serological and molecular survey of Leptospira spp. among cart horses from an endemic area of human leptospirosis in Curitiba, southern Brazil
title_full Serological and molecular survey of Leptospira spp. among cart horses from an endemic area of human leptospirosis in Curitiba, southern Brazil
title_fullStr Serological and molecular survey of Leptospira spp. among cart horses from an endemic area of human leptospirosis in Curitiba, southern Brazil
title_full_unstemmed Serological and molecular survey of Leptospira spp. among cart horses from an endemic area of human leptospirosis in Curitiba, southern Brazil
title_sort Serological and molecular survey of Leptospira spp. among cart horses from an endemic area of human leptospirosis in Curitiba, southern Brazil
author Finger, Mariane Angelica
author_facet Finger, Mariane Angelica
Barros Filho, Ivan Roque de
Leutenegger, Christian
Estrada, Marko
Ullmann, Leila Sabrina [UNESP]
Langoni, Helio [UNESP]
Kikuti, Mariana [UNESP]
Dornbush, Peterson Triches
Deconto, Ivan
Biondo, Alexander Welker
author_role author
author2 Barros Filho, Ivan Roque de
Leutenegger, Christian
Estrada, Marko
Ullmann, Leila Sabrina [UNESP]
Langoni, Helio [UNESP]
Kikuti, Mariana [UNESP]
Dornbush, Peterson Triches
Deconto, Ivan
Biondo, Alexander Welker
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal do Paraná (UFPR)
University of Illinois
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Finger, Mariane Angelica
Barros Filho, Ivan Roque de
Leutenegger, Christian
Estrada, Marko
Ullmann, Leila Sabrina [UNESP]
Langoni, Helio [UNESP]
Kikuti, Mariana [UNESP]
Dornbush, Peterson Triches
Deconto, Ivan
Biondo, Alexander Welker
dc.subject.por.fl_str_mv Leptospira spp.
MAT
qPCR
Horses
topic Leptospira spp.
MAT
qPCR
Horses
description Introduction: Cart horses are a re-emerging population employed to carry recyclable material in cities. Methods: Sixty-two horses were sampled in an endemic area of human leptospirosis. The microscopic agglutination test (MAT) and real-time polymerase chain reaction (qPCR) were performed. Results: A seropositivity of 75.8% with serovar Icterohaemorrhagiae in 80.8% of the horses was observed. Blood and urine were qPCR negative. MAT showed positive correlations with rainfall (p = 0.02) and flooding (p = 0.03). Conclusions: Although horses may be constantly exposed to Leptospira spp. in the environment mostly because of rainfall and flooding, no leptospiremia or leptospiruria were observed in this study.
publishDate 2014
dc.date.none.fl_str_mv 2014-11-01
2015-11-03T15:27:45Z
2015-11-03T15:27:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652014000600473&lng=en&nrm=iso&tlng=en
Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo: Inst Medicina Tropical Sao Paulo, v. 56, n. 6, p. 473-476, 2014.
0036-4665
http://hdl.handle.net/11449/129914
10.1590/S0036-46652014000600003
S0036-46652014000600473
WOS:000344333000003
S0036-46652014000600473.pdf
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652014000600473&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/129914
identifier_str_mv Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo: Inst Medicina Tropical Sao Paulo, v. 56, n. 6, p. 473-476, 2014.
0036-4665
10.1590/S0036-46652014000600003
S0036-46652014000600473
WOS:000344333000003
S0036-46652014000600473.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Do Instituto De Medicina Tropical De Sao Paulo
1.489
0,669
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 473-476
application/pdf
dc.publisher.none.fl_str_mv Inst Medicina Tropical Sao Paulo
publisher.none.fl_str_mv Inst Medicina Tropical Sao Paulo
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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