Inteins in pathogenic fungi: a phylogenetic tool and perspectives for therapeutic applications
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0074-02762009000300017 http://hdl.handle.net/11449/18130 |
Resumo: | Inteins or internal proteins are coding sequences that are transcribed and translated with flanking sequences (exteins). After translation, the inteins are excised by an autocatalytic process and the host protein assumes its normal conformation and develops its expected function. These parasitic genetic elements have been found in important, conserved proteins in all three domains of life. Most of the eukaryotic inteins are present in the fungi kingdom and the PRP8 intein is one of the most widespread inteins, occurring in important pathogens such as Cryptococcus neoformans (varieties grubii and neoformans), Cryptococcus gattii, Histoplasma capsulatum and Paracoccidioides brasiliensis. The knowledge of conserved and non-conserved domains in inteins have opened up new opportunities for the study of population variability in pathogenic fungi, including their phylogenetic relationships and recognition or diagnoses of species. Furthermore, inteins in pathogenic fungi should also be considered a promising therapeutic drug target, since once the autocatalytic splicing is inhibited, the host protein, which is typically vital, will not be able to perform its normal function and the fungal cell will not survive or reproduce. |
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Repositório Institucional da UNESP |
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Inteins in pathogenic fungi: a phylogenetic tool and perspectives for therapeutic applicationsInteinPRP8 speciespathogenic fungitherapeutic targetInteins or internal proteins are coding sequences that are transcribed and translated with flanking sequences (exteins). After translation, the inteins are excised by an autocatalytic process and the host protein assumes its normal conformation and develops its expected function. These parasitic genetic elements have been found in important, conserved proteins in all three domains of life. Most of the eukaryotic inteins are present in the fungi kingdom and the PRP8 intein is one of the most widespread inteins, occurring in important pathogens such as Cryptococcus neoformans (varieties grubii and neoformans), Cryptococcus gattii, Histoplasma capsulatum and Paracoccidioides brasiliensis. The knowledge of conserved and non-conserved domains in inteins have opened up new opportunities for the study of population variability in pathogenic fungi, including their phylogenetic relationships and recognition or diagnoses of species. Furthermore, inteins in pathogenic fungi should also be considered a promising therapeutic drug target, since once the autocatalytic splicing is inhibited, the host protein, which is typically vital, will not be able to perform its normal function and the fungal cell will not survive or reproduce.UNESP Instituto de Biociências Departamento de Microbiologia e Imunologia, BotucatuUNESP Instituto de Biociências Departamento de Microbiologia e Imunologia, BotucatuInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade Estadual Paulista (Unesp)Theodoro, Raquel Cordeiro [UNESP]Bagagli, Eduardo [UNESP]2014-05-20T13:50:48Z2014-05-20T13:50:48Z2009-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article497-504application/pdfhttp://dx.doi.org/10.1590/S0074-02762009000300017Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 104, n. 3, p. 497-504, 2009.0074-0276http://hdl.handle.net/11449/1813010.1590/S0074-02762009000300017S0074-02762009000300017WOS:000267332000017S0074-02762009000300017.pdf33203275704295390000-0002-8003-4109SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2023-10-08T06:05:56Zoai:repositorio.unesp.br:11449/18130Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:20:03.961752Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Inteins in pathogenic fungi: a phylogenetic tool and perspectives for therapeutic applications |
title |
Inteins in pathogenic fungi: a phylogenetic tool and perspectives for therapeutic applications |
spellingShingle |
Inteins in pathogenic fungi: a phylogenetic tool and perspectives for therapeutic applications Theodoro, Raquel Cordeiro [UNESP] Intein PRP8 species pathogenic fungi therapeutic target |
title_short |
Inteins in pathogenic fungi: a phylogenetic tool and perspectives for therapeutic applications |
title_full |
Inteins in pathogenic fungi: a phylogenetic tool and perspectives for therapeutic applications |
title_fullStr |
Inteins in pathogenic fungi: a phylogenetic tool and perspectives for therapeutic applications |
title_full_unstemmed |
Inteins in pathogenic fungi: a phylogenetic tool and perspectives for therapeutic applications |
title_sort |
Inteins in pathogenic fungi: a phylogenetic tool and perspectives for therapeutic applications |
author |
Theodoro, Raquel Cordeiro [UNESP] |
author_facet |
Theodoro, Raquel Cordeiro [UNESP] Bagagli, Eduardo [UNESP] |
author_role |
author |
author2 |
Bagagli, Eduardo [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Theodoro, Raquel Cordeiro [UNESP] Bagagli, Eduardo [UNESP] |
dc.subject.por.fl_str_mv |
Intein PRP8 species pathogenic fungi therapeutic target |
topic |
Intein PRP8 species pathogenic fungi therapeutic target |
description |
Inteins or internal proteins are coding sequences that are transcribed and translated with flanking sequences (exteins). After translation, the inteins are excised by an autocatalytic process and the host protein assumes its normal conformation and develops its expected function. These parasitic genetic elements have been found in important, conserved proteins in all three domains of life. Most of the eukaryotic inteins are present in the fungi kingdom and the PRP8 intein is one of the most widespread inteins, occurring in important pathogens such as Cryptococcus neoformans (varieties grubii and neoformans), Cryptococcus gattii, Histoplasma capsulatum and Paracoccidioides brasiliensis. The knowledge of conserved and non-conserved domains in inteins have opened up new opportunities for the study of population variability in pathogenic fungi, including their phylogenetic relationships and recognition or diagnoses of species. Furthermore, inteins in pathogenic fungi should also be considered a promising therapeutic drug target, since once the autocatalytic splicing is inhibited, the host protein, which is typically vital, will not be able to perform its normal function and the fungal cell will not survive or reproduce. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-05-01 2014-05-20T13:50:48Z 2014-05-20T13:50:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0074-02762009000300017 Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 104, n. 3, p. 497-504, 2009. 0074-0276 http://hdl.handle.net/11449/18130 10.1590/S0074-02762009000300017 S0074-02762009000300017 WOS:000267332000017 S0074-02762009000300017.pdf 3320327570429539 0000-0002-8003-4109 |
url |
http://dx.doi.org/10.1590/S0074-02762009000300017 http://hdl.handle.net/11449/18130 |
identifier_str_mv |
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 104, n. 3, p. 497-504, 2009. 0074-0276 10.1590/S0074-02762009000300017 S0074-02762009000300017 WOS:000267332000017 S0074-02762009000300017.pdf 3320327570429539 0000-0002-8003-4109 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz 2.833 1,172 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
497-504 application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128348824535040 |