Cytotoxic and genotoxic effects of macroptilium lathyroides

Detalhes bibliográficos
Autor(a) principal: Silva, M. S.A.
Data de Publicação: 2018
Outros Autores: Yamashita, O. M., Rossi, A. A.B., Concenço, G., Carvalho, M. A.C., Felito, R. A. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-83582018360100043
http://hdl.handle.net/11449/171216
Resumo: The monitoring of phytochemicals with potentially toxic properties can be assessed by observing the physiological and cellular alterations of the test organism exposed. This present study aimed to evaluate the cytotoxic and genotoxic potential of aqueous extracts of leaves and roots of Macroptilium lathyroides weed on the cell cycle of lettuce. Bioassays were conducted in a germinator (25 °C) with aqueous extract at concentrations of 0, 5, 10, 20 and 40% p v-1, obtained from fresh leaves and roots. For biological, lettuce root meristems were used for the preparation of slides using the technique of squashing. All blades were observed with an optical microscope at a magnitude of 400x. A total of 5,000 cells were analyzed for each treatment, and the number of cells in each phase of mitosis was recorded. Possible presence of chromosomal abnormalities was verified, such as chromatid breaks, anafasic bridges, loss of whole chromosomes or micronuclei formation. These analyzes were conducted only on a qualitative level. Results showed that aqueous extracts of M. lathyroides mitotic caused reduced index with increased concentration. Genotoxic activity was also observed for both extracts tested, since composition resulted in cell cycle changes and chromosomal abnormalities.
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spelling Cytotoxic and genotoxic effects of macroptilium lathyroidesEfeito citotóxico e Genotóxico da espécie macroptilium lathyroidesAbnormalitiesBioassaysCell divisionMitotic indexPhasey beanThe monitoring of phytochemicals with potentially toxic properties can be assessed by observing the physiological and cellular alterations of the test organism exposed. This present study aimed to evaluate the cytotoxic and genotoxic potential of aqueous extracts of leaves and roots of Macroptilium lathyroides weed on the cell cycle of lettuce. Bioassays were conducted in a germinator (25 °C) with aqueous extract at concentrations of 0, 5, 10, 20 and 40% p v-1, obtained from fresh leaves and roots. For biological, lettuce root meristems were used for the preparation of slides using the technique of squashing. All blades were observed with an optical microscope at a magnitude of 400x. A total of 5,000 cells were analyzed for each treatment, and the number of cells in each phase of mitosis was recorded. Possible presence of chromosomal abnormalities was verified, such as chromatid breaks, anafasic bridges, loss of whole chromosomes or micronuclei formation. These analyzes were conducted only on a qualitative level. Results showed that aqueous extracts of M. lathyroides mitotic caused reduced index with increased concentration. Genotoxic activity was also observed for both extracts tested, since composition resulted in cell cycle changes and chromosomal abnormalities.Fundação de Amparo à Pesquisa do Estado de Mato GrossoUNEMATEMBRAPA Clima TemperadoUniversidade Estadual Paulista UNESPUniversidade Estadual Paulista UNESPUNEMATEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Silva, M. S.A.Yamashita, O. M.Rossi, A. A.B.Concenço, G.Carvalho, M. A.C.Felito, R. A. [UNESP]2018-12-11T16:54:26Z2018-12-11T16:54:26Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/S0100-83582018360100043Planta Daninha, v. 36.0100-8358http://hdl.handle.net/11449/17121610.1590/S0100-83582018360100043S0100-835820180001002442-s2.0-85049862040S0100-83582018000100244.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlanta Daninha0,365info:eu-repo/semantics/openAccess2024-01-22T06:20:40Zoai:repositorio.unesp.br:11449/171216Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:38:08.874466Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cytotoxic and genotoxic effects of macroptilium lathyroides
Efeito citotóxico e Genotóxico da espécie macroptilium lathyroides
title Cytotoxic and genotoxic effects of macroptilium lathyroides
spellingShingle Cytotoxic and genotoxic effects of macroptilium lathyroides
Silva, M. S.A.
Abnormalities
Bioassays
Cell division
Mitotic index
Phasey bean
title_short Cytotoxic and genotoxic effects of macroptilium lathyroides
title_full Cytotoxic and genotoxic effects of macroptilium lathyroides
title_fullStr Cytotoxic and genotoxic effects of macroptilium lathyroides
title_full_unstemmed Cytotoxic and genotoxic effects of macroptilium lathyroides
title_sort Cytotoxic and genotoxic effects of macroptilium lathyroides
author Silva, M. S.A.
author_facet Silva, M. S.A.
Yamashita, O. M.
Rossi, A. A.B.
Concenço, G.
Carvalho, M. A.C.
Felito, R. A. [UNESP]
author_role author
author2 Yamashita, O. M.
Rossi, A. A.B.
Concenço, G.
Carvalho, M. A.C.
Felito, R. A. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv UNEMAT
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Silva, M. S.A.
Yamashita, O. M.
Rossi, A. A.B.
Concenço, G.
Carvalho, M. A.C.
Felito, R. A. [UNESP]
dc.subject.por.fl_str_mv Abnormalities
Bioassays
Cell division
Mitotic index
Phasey bean
topic Abnormalities
Bioassays
Cell division
Mitotic index
Phasey bean
description The monitoring of phytochemicals with potentially toxic properties can be assessed by observing the physiological and cellular alterations of the test organism exposed. This present study aimed to evaluate the cytotoxic and genotoxic potential of aqueous extracts of leaves and roots of Macroptilium lathyroides weed on the cell cycle of lettuce. Bioassays were conducted in a germinator (25 °C) with aqueous extract at concentrations of 0, 5, 10, 20 and 40% p v-1, obtained from fresh leaves and roots. For biological, lettuce root meristems were used for the preparation of slides using the technique of squashing. All blades were observed with an optical microscope at a magnitude of 400x. A total of 5,000 cells were analyzed for each treatment, and the number of cells in each phase of mitosis was recorded. Possible presence of chromosomal abnormalities was verified, such as chromatid breaks, anafasic bridges, loss of whole chromosomes or micronuclei formation. These analyzes were conducted only on a qualitative level. Results showed that aqueous extracts of M. lathyroides mitotic caused reduced index with increased concentration. Genotoxic activity was also observed for both extracts tested, since composition resulted in cell cycle changes and chromosomal abnormalities.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T16:54:26Z
2018-12-11T16:54:26Z
2018-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-83582018360100043
Planta Daninha, v. 36.
0100-8358
http://hdl.handle.net/11449/171216
10.1590/S0100-83582018360100043
S0100-83582018000100244
2-s2.0-85049862040
S0100-83582018000100244.pdf
url http://dx.doi.org/10.1590/S0100-83582018360100043
http://hdl.handle.net/11449/171216
identifier_str_mv Planta Daninha, v. 36.
0100-8358
10.1590/S0100-83582018360100043
S0100-83582018000100244
2-s2.0-85049862040
S0100-83582018000100244.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Planta Daninha
0,365
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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