Genetic Diversity of a Brazilian Strain Collection of Xanthomonas citri subsp citri Based on the Type III Effector Protein Genes
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1094/PDIS-04-11-0357 http://hdl.handle.net/11449/4070 |
Resumo: | Jaciani, F. J., Ferro, J. A., Ferro, M. I. T., Verniere, C., Pruvost, O., and Belasque, J., Jr. 2012. Genetic diversity of a Brazilian strain collection of Xanthomonas citri subsp. citri based on the type III effector protein genes. Plant Dis. 96:193-203.Exclusion and eradication or management based on an integrated approach with less susceptible varieties, copper-based bactericides, and windbreaks are the two main strategies used to prevent or control citrus canker. Field tolerance or resistance to citrus canker is not found in the most important commercial sweet orange cultivars, and pathogen-derived resistance has been developed and applied in different crops to obtain resistant genotypes to plant pathogens. We describe the development of DNA primers and probes based on the type III effector genes avrXacE1, avrXacE2, avrXacE3, avrBs2, pthA4, hpaF, and XAC3090 (leucine rich protein), and their application in the evaluation of the genetic diversity of the pathogen. A total of 49 haplotypes were identified in 157 strains by Southern blot analysis. No genetic polymorphism was detected by BOX elements - and enterobacterial repetitive inter-genic consensus polymerase chain reaction (ERIC-PCR) analysis, nor with the genes avrBs2, XAC3090, and hpaF. Nei's genetic diversity indexes varied from 0.65 to 0.96 for subcollections of the pathogen. One or few haplotypes were most frequent in the strain collection, but several haplotypes were represented by solely one or few strains. The PthA4 probe resulted in the higher number of haplotypes identified in the Brazilian subcollections. Greater variation in the frequency of haplotypes occurred within subcollections (93.7%) than among subcollections. Only some haplotypes were genetically distant from all others, especially those originated from Rio Grande do Sul and Santa Catarina states. These bacterial effectors are widely spread in the collections and are useful for a better understanding of the host pathogen interaction and the search for resistance genes in host and nonhost plants. |
id |
UNSP_fee561e1a259630af6484668af5cdc48 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/4070 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Genetic Diversity of a Brazilian Strain Collection of Xanthomonas citri subsp citri Based on the Type III Effector Protein GenesJaciani, F. J., Ferro, J. A., Ferro, M. I. T., Verniere, C., Pruvost, O., and Belasque, J., Jr. 2012. Genetic diversity of a Brazilian strain collection of Xanthomonas citri subsp. citri based on the type III effector protein genes. Plant Dis. 96:193-203.Exclusion and eradication or management based on an integrated approach with less susceptible varieties, copper-based bactericides, and windbreaks are the two main strategies used to prevent or control citrus canker. Field tolerance or resistance to citrus canker is not found in the most important commercial sweet orange cultivars, and pathogen-derived resistance has been developed and applied in different crops to obtain resistant genotypes to plant pathogens. We describe the development of DNA primers and probes based on the type III effector genes avrXacE1, avrXacE2, avrXacE3, avrBs2, pthA4, hpaF, and XAC3090 (leucine rich protein), and their application in the evaluation of the genetic diversity of the pathogen. A total of 49 haplotypes were identified in 157 strains by Southern blot analysis. No genetic polymorphism was detected by BOX elements - and enterobacterial repetitive inter-genic consensus polymerase chain reaction (ERIC-PCR) analysis, nor with the genes avrBs2, XAC3090, and hpaF. Nei's genetic diversity indexes varied from 0.65 to 0.96 for subcollections of the pathogen. One or few haplotypes were most frequent in the strain collection, but several haplotypes were represented by solely one or few strains. The PthA4 probe resulted in the higher number of haplotypes identified in the Brazilian subcollections. Greater variation in the frequency of haplotypes occurred within subcollections (93.7%) than among subcollections. Only some haplotypes were genetically distant from all others, especially those originated from Rio Grande do Sul and Santa Catarina states. These bacterial effectors are widely spread in the collections and are useful for a better understanding of the host pathogen interaction and the search for resistance genes in host and nonhost plants.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundo Def Citricultura, Araraquara, SP, BrazilUNESP Univ Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet, Jaboticabal, SP, BrazilUniv Reunion, CIRAD, UMR Peuplements Vegetaux & Bioagresseurs Milieu T, St Pierre 97410, ReunionUNESP Univ Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet, Jaboticabal, SP, BrazilFAPESP: 07/01431-4CNPq: 300231/2008-3Amer Phytopathological SocFundo Def CitriculturaUniversidade Estadual Paulista (Unesp)Univ ReunionJaciani, F. J. [UNESP]Ferro, J. A. [UNESP]Ferro, M. I. T. [UNESP]Verniere, C.Pruvost, O.Belasque, J.2014-05-20T13:17:42Z2014-05-20T13:17:42Z2012-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article193-203http://dx.doi.org/10.1094/PDIS-04-11-0357Plant Disease. St Paul: Amer Phytopathological Soc, v. 96, n. 2, p. 193-203, 2012.0191-2917http://hdl.handle.net/11449/407010.1094/PDIS-04-11-0357WOS:0002993949000030147241723612464Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlant Disease2.9410,575info:eu-repo/semantics/openAccess2024-06-07T15:31:59Zoai:repositorio.unesp.br:11449/4070Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:32:09.819165Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic Diversity of a Brazilian Strain Collection of Xanthomonas citri subsp citri Based on the Type III Effector Protein Genes |
title |
Genetic Diversity of a Brazilian Strain Collection of Xanthomonas citri subsp citri Based on the Type III Effector Protein Genes |
spellingShingle |
Genetic Diversity of a Brazilian Strain Collection of Xanthomonas citri subsp citri Based on the Type III Effector Protein Genes Jaciani, F. J. [UNESP] |
title_short |
Genetic Diversity of a Brazilian Strain Collection of Xanthomonas citri subsp citri Based on the Type III Effector Protein Genes |
title_full |
Genetic Diversity of a Brazilian Strain Collection of Xanthomonas citri subsp citri Based on the Type III Effector Protein Genes |
title_fullStr |
Genetic Diversity of a Brazilian Strain Collection of Xanthomonas citri subsp citri Based on the Type III Effector Protein Genes |
title_full_unstemmed |
Genetic Diversity of a Brazilian Strain Collection of Xanthomonas citri subsp citri Based on the Type III Effector Protein Genes |
title_sort |
Genetic Diversity of a Brazilian Strain Collection of Xanthomonas citri subsp citri Based on the Type III Effector Protein Genes |
author |
Jaciani, F. J. [UNESP] |
author_facet |
Jaciani, F. J. [UNESP] Ferro, J. A. [UNESP] Ferro, M. I. T. [UNESP] Verniere, C. Pruvost, O. Belasque, J. |
author_role |
author |
author2 |
Ferro, J. A. [UNESP] Ferro, M. I. T. [UNESP] Verniere, C. Pruvost, O. Belasque, J. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Fundo Def Citricultura Universidade Estadual Paulista (Unesp) Univ Reunion |
dc.contributor.author.fl_str_mv |
Jaciani, F. J. [UNESP] Ferro, J. A. [UNESP] Ferro, M. I. T. [UNESP] Verniere, C. Pruvost, O. Belasque, J. |
description |
Jaciani, F. J., Ferro, J. A., Ferro, M. I. T., Verniere, C., Pruvost, O., and Belasque, J., Jr. 2012. Genetic diversity of a Brazilian strain collection of Xanthomonas citri subsp. citri based on the type III effector protein genes. Plant Dis. 96:193-203.Exclusion and eradication or management based on an integrated approach with less susceptible varieties, copper-based bactericides, and windbreaks are the two main strategies used to prevent or control citrus canker. Field tolerance or resistance to citrus canker is not found in the most important commercial sweet orange cultivars, and pathogen-derived resistance has been developed and applied in different crops to obtain resistant genotypes to plant pathogens. We describe the development of DNA primers and probes based on the type III effector genes avrXacE1, avrXacE2, avrXacE3, avrBs2, pthA4, hpaF, and XAC3090 (leucine rich protein), and their application in the evaluation of the genetic diversity of the pathogen. A total of 49 haplotypes were identified in 157 strains by Southern blot analysis. No genetic polymorphism was detected by BOX elements - and enterobacterial repetitive inter-genic consensus polymerase chain reaction (ERIC-PCR) analysis, nor with the genes avrBs2, XAC3090, and hpaF. Nei's genetic diversity indexes varied from 0.65 to 0.96 for subcollections of the pathogen. One or few haplotypes were most frequent in the strain collection, but several haplotypes were represented by solely one or few strains. The PthA4 probe resulted in the higher number of haplotypes identified in the Brazilian subcollections. Greater variation in the frequency of haplotypes occurred within subcollections (93.7%) than among subcollections. Only some haplotypes were genetically distant from all others, especially those originated from Rio Grande do Sul and Santa Catarina states. These bacterial effectors are widely spread in the collections and are useful for a better understanding of the host pathogen interaction and the search for resistance genes in host and nonhost plants. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-02-01 2014-05-20T13:17:42Z 2014-05-20T13:17:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1094/PDIS-04-11-0357 Plant Disease. St Paul: Amer Phytopathological Soc, v. 96, n. 2, p. 193-203, 2012. 0191-2917 http://hdl.handle.net/11449/4070 10.1094/PDIS-04-11-0357 WOS:000299394900003 0147241723612464 |
url |
http://dx.doi.org/10.1094/PDIS-04-11-0357 http://hdl.handle.net/11449/4070 |
identifier_str_mv |
Plant Disease. St Paul: Amer Phytopathological Soc, v. 96, n. 2, p. 193-203, 2012. 0191-2917 10.1094/PDIS-04-11-0357 WOS:000299394900003 0147241723612464 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plant Disease 2.941 0,575 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
193-203 |
dc.publisher.none.fl_str_mv |
Amer Phytopathological Soc |
publisher.none.fl_str_mv |
Amer Phytopathological Soc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128943525462016 |