Polimorfismo da beta-lactoglobulina não afeta as caracterí sticas físico-químicas e a estabilidade do leite bovino
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-204X2007000500019 http://hdl.handle.net/11449/69640 |
Resumo: | The objective of this study was to evaluate the effect of beta-lactoglobulin polymorphism, breed and seasonality on physico-chemical characteristics and stability of bovine milk. Six Holstein-Zebu (H-Z) and five Holstein dairy herds were selected, of which 660 Holstein and 293 crossbred Holstein-Zebu milk and blood samples were collected, adding up 953 samples, distributed in two collecting periods at rainy and two at dry seasons. Each milk sample was analyzed for titratable acidity, pH, freezing point and milk ethanol stability, at the following ethanol concentrations: 70, 76, 80 and 84GL. Individual blood samples were submitted to polymerase chain reaction (PCR) for the determination of beta-lactoglobulin polymorphism. No effect of beta-lactoglobulin polymorphism was observed in physico-chemical characteristics of milk, in both studied breed. Breed effect for Holstein and H-Z, respectively, was observed on titratable acidity (16.16 and 17.07°D, while effect of seasonality (for rainy and dry seasons, respectively) was also observed on freezing point (-0.5411 and -0.5376°H). Effects of breed and seasonality on milk stability were observed (Holstein-Zebu milk was less stable on dry season), however, no effect of beta-lactoglobulin on milk stability was observed. |
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Polimorfismo da beta-lactoglobulina não afeta as caracterí sticas físico-químicas e a estabilidade do leite bovinoBeta-lactoglobulin polymorphism does not affect physico-chemical characteristics and milk stabilityDairy cowEthanol stability testGenetic variantsMilk physico-chemical propertiesBosBos indicusBovinaeThe objective of this study was to evaluate the effect of beta-lactoglobulin polymorphism, breed and seasonality on physico-chemical characteristics and stability of bovine milk. Six Holstein-Zebu (H-Z) and five Holstein dairy herds were selected, of which 660 Holstein and 293 crossbred Holstein-Zebu milk and blood samples were collected, adding up 953 samples, distributed in two collecting periods at rainy and two at dry seasons. Each milk sample was analyzed for titratable acidity, pH, freezing point and milk ethanol stability, at the following ethanol concentrations: 70, 76, 80 and 84GL. Individual blood samples were submitted to polymerase chain reaction (PCR) for the determination of beta-lactoglobulin polymorphism. No effect of beta-lactoglobulin polymorphism was observed in physico-chemical characteristics of milk, in both studied breed. Breed effect for Holstein and H-Z, respectively, was observed on titratable acidity (16.16 and 17.07°D, while effect of seasonality (for rainy and dry seasons, respectively) was also observed on freezing point (-0.5411 and -0.5376°H). Effects of breed and seasonality on milk stability were observed (Holstein-Zebu milk was less stable on dry season), however, no effect of beta-lactoglobulin on milk stability was observed.O objetivo deste trabalho foi avaliar o efeito do polimorfismo genético da beta-lactoglobulina, da raça e da sazonalidade sobre as características físico-químicas e estabilidade do leite bovino. Foram selecionados 5 rebanhos da raça Holandesa e 6 da Girolando. Amostras de leite e sangue foram coletadas de 660 vacas Holandesas e 293 Girolandos, num total de 953 amostras, obtidas em duas coletas na estação seca e duas na estação chuvosa. As amostras de leite foram submetidas à análise de acidez titulável, pH, crioscopia, e ao teste de estabilidade ao etanol (70, 76, 80 e 84ºGL). As amostras de sangue foram submetidas à reação em cadeia de polimerase, para determinação do polimorfismo da beta-lactoglobulina em ambas as raças estudadas. Não houve efeito do polimorfismo da beta-lactoglobulina sobre as características físico-químicas do leite. Observou-se efeito de raça (Holandesa e Girolando, respectivamente) sobre a acidez titulável (16,16 e 17,07°D), e da sazonalidade (estações chuvosa e seca, respectivamente) sobre a crioscopia (-0,5411 e -0,5376°H). Nas condições do estudo, observou-se efeito de raça e sazonalidade sobre a estabilidade do leite, com maior instabilidade do leite de Girolando, durante a estação seca. Não houve efeito do polimorfismo da beta-lactoglobulina sobre essa característica.Universidade de São Paulo Fac. de Medicina Veterinária e Zootecnia, Av. Duque de Caxias Norte, 225, CEP 13635-900 Pirassununga, SPCentro Universitário Anhanguera Fac. de Medicina Veterinária, R. Waldemar Silenci, 340, CEP 13614-370 Leme, SPUniversidade Federal de Lavras Fac. de Zootecnia, Caixa Postal 3037, CEP 37200-000 Lavras, MGUniversidade Estadual Paulista Júlio de Mesquita Filho Laboratório de Bioquímica e Biologia Molecular Animal, R. Clóvis Pestana, 793, CEP 16050-680 Araçatuba, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Laboratório de Bioquímica e Biologia Molecular Animal, R. Clóvis Pestana, 793, CEP 16050-680 Araçatuba, SPUniversidade de São Paulo (USP)Fac. de Medicina VeterináriaUniversidade Federal de Lavras (UFLA)Universidade Estadual Paulista (Unesp)Botaro, Bruno GarciaDe Lima, Ygor Vinícius RealAquino, Adriana AugustoFernandes, Raquel Helena RochaGarcia, José Fernando [UNESP]Dos Santos, Marcos Veiga2014-05-27T11:22:27Z2014-05-27T11:22:27Z2007-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article747-753application/pdfhttp://dx.doi.org/10.1590/S0100-204X2007000500019Pesquisa Agropecuaria Brasileira, v. 42, n. 5, p. 747-753, 2007.0100-204X1678-3921http://hdl.handle.net/11449/6964010.1590/S0100-204X2007000500019S0100-204X2007000500019WOS:0002475655000192-s2.0-343473543712-s2.0-34347354371.pdf9991374083045897Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporPesquisa Agropecuária Brasileira0.5460,469info:eu-repo/semantics/openAccess2024-09-04T19:15:11Zoai:repositorio.unesp.br:11449/69640Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Polimorfismo da beta-lactoglobulina não afeta as caracterí sticas físico-químicas e a estabilidade do leite bovino Beta-lactoglobulin polymorphism does not affect physico-chemical characteristics and milk stability |
title |
Polimorfismo da beta-lactoglobulina não afeta as caracterí sticas físico-químicas e a estabilidade do leite bovino |
spellingShingle |
Polimorfismo da beta-lactoglobulina não afeta as caracterí sticas físico-químicas e a estabilidade do leite bovino Botaro, Bruno Garcia Dairy cow Ethanol stability test Genetic variants Milk physico-chemical properties Bos Bos indicus Bovinae |
title_short |
Polimorfismo da beta-lactoglobulina não afeta as caracterí sticas físico-químicas e a estabilidade do leite bovino |
title_full |
Polimorfismo da beta-lactoglobulina não afeta as caracterí sticas físico-químicas e a estabilidade do leite bovino |
title_fullStr |
Polimorfismo da beta-lactoglobulina não afeta as caracterí sticas físico-químicas e a estabilidade do leite bovino |
title_full_unstemmed |
Polimorfismo da beta-lactoglobulina não afeta as caracterí sticas físico-químicas e a estabilidade do leite bovino |
title_sort |
Polimorfismo da beta-lactoglobulina não afeta as caracterí sticas físico-químicas e a estabilidade do leite bovino |
author |
Botaro, Bruno Garcia |
author_facet |
Botaro, Bruno Garcia De Lima, Ygor Vinícius Real Aquino, Adriana Augusto Fernandes, Raquel Helena Rocha Garcia, José Fernando [UNESP] Dos Santos, Marcos Veiga |
author_role |
author |
author2 |
De Lima, Ygor Vinícius Real Aquino, Adriana Augusto Fernandes, Raquel Helena Rocha Garcia, José Fernando [UNESP] Dos Santos, Marcos Veiga |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Fac. de Medicina Veterinária Universidade Federal de Lavras (UFLA) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Botaro, Bruno Garcia De Lima, Ygor Vinícius Real Aquino, Adriana Augusto Fernandes, Raquel Helena Rocha Garcia, José Fernando [UNESP] Dos Santos, Marcos Veiga |
dc.subject.por.fl_str_mv |
Dairy cow Ethanol stability test Genetic variants Milk physico-chemical properties Bos Bos indicus Bovinae |
topic |
Dairy cow Ethanol stability test Genetic variants Milk physico-chemical properties Bos Bos indicus Bovinae |
description |
The objective of this study was to evaluate the effect of beta-lactoglobulin polymorphism, breed and seasonality on physico-chemical characteristics and stability of bovine milk. Six Holstein-Zebu (H-Z) and five Holstein dairy herds were selected, of which 660 Holstein and 293 crossbred Holstein-Zebu milk and blood samples were collected, adding up 953 samples, distributed in two collecting periods at rainy and two at dry seasons. Each milk sample was analyzed for titratable acidity, pH, freezing point and milk ethanol stability, at the following ethanol concentrations: 70, 76, 80 and 84GL. Individual blood samples were submitted to polymerase chain reaction (PCR) for the determination of beta-lactoglobulin polymorphism. No effect of beta-lactoglobulin polymorphism was observed in physico-chemical characteristics of milk, in both studied breed. Breed effect for Holstein and H-Z, respectively, was observed on titratable acidity (16.16 and 17.07°D, while effect of seasonality (for rainy and dry seasons, respectively) was also observed on freezing point (-0.5411 and -0.5376°H). Effects of breed and seasonality on milk stability were observed (Holstein-Zebu milk was less stable on dry season), however, no effect of beta-lactoglobulin on milk stability was observed. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-05-01 2014-05-27T11:22:27Z 2014-05-27T11:22:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-204X2007000500019 Pesquisa Agropecuaria Brasileira, v. 42, n. 5, p. 747-753, 2007. 0100-204X 1678-3921 http://hdl.handle.net/11449/69640 10.1590/S0100-204X2007000500019 S0100-204X2007000500019 WOS:000247565500019 2-s2.0-34347354371 2-s2.0-34347354371.pdf 9991374083045897 |
url |
http://dx.doi.org/10.1590/S0100-204X2007000500019 http://hdl.handle.net/11449/69640 |
identifier_str_mv |
Pesquisa Agropecuaria Brasileira, v. 42, n. 5, p. 747-753, 2007. 0100-204X 1678-3921 10.1590/S0100-204X2007000500019 S0100-204X2007000500019 WOS:000247565500019 2-s2.0-34347354371 2-s2.0-34347354371.pdf 9991374083045897 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Pesquisa Agropecuária Brasileira 0.546 0,469 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
747-753 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021369137594368 |