Suprachiasmatic Nucleus and Subordinate Brain Oscillators: Clock Gene Desynchronization by Neuroinflammation
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1159/000484931 http://hdl.handle.net/11449/179488 |
Resumo: | Objective: The clock genes Period (per) 1 and 2 are essential components in the generation and adjustment of biological circadian rhythms by the suprachiasmatic nucleus (SCN). Both genes are also rhythmically present in extrahypothalamic areas such as the hippocampus and cerebellum, considered subordinate oscillators. Several pathological conditions alter rhythmic biological phenomena, but the mechanisms behind these changes involving the clock genes are not well defined. The current study investigated changes in PER1 and PER2 immunoreactivity in the SCN, hippocampus, and cerebellum in a neuroinflammation model. Methods: Wistar rats received lipopolysaccharide (LPS) or vehicle intracerebroventricularly. The melatonin plasmatic content was quantified by ELISA to confirm the alterations in biological rhythms, and PER1 and PER2 immunoreactivities were analyzed in brain sections by immunohistochemistry. Results: In the SCN, intracerebroventricular LPS changed PER1 expression, increasing the number of PER1-immunoreactive (IR) cells at zeitgeber time (ZT) 15, decreasing it at ZT5 and ZT20 and not changing it at ZT10. LPS also induced a decrease in PER2-IR cells at ZT5, ZT10, and ZT15 but not at ZT20 in the SCN. In the hippocampus, LPS induced a decrease in PER1-IR and PER2-IR cells at both ZTs (ZT10 and ZT15). In the cerebellum, LPS increased the number of PER1-IR cells at ZT10 and decreased it at ZT15, while the number of PER2-IR cells was reduced at both ZTs. Conclusions: These results indicate that a neuroinflammatory condition leads to desynchronization of primary and subordinate brain oscillators, supporting the existence of the integration between the immune and the circadian system. |
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Repositório Institucional da UNESP |
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Suprachiasmatic Nucleus and Subordinate Brain Oscillators: Clock Gene Desynchronization by NeuroinflammationCircadian rhythmsClock genesNeuroinflammationOscillatorsObjective: The clock genes Period (per) 1 and 2 are essential components in the generation and adjustment of biological circadian rhythms by the suprachiasmatic nucleus (SCN). Both genes are also rhythmically present in extrahypothalamic areas such as the hippocampus and cerebellum, considered subordinate oscillators. Several pathological conditions alter rhythmic biological phenomena, but the mechanisms behind these changes involving the clock genes are not well defined. The current study investigated changes in PER1 and PER2 immunoreactivity in the SCN, hippocampus, and cerebellum in a neuroinflammation model. Methods: Wistar rats received lipopolysaccharide (LPS) or vehicle intracerebroventricularly. The melatonin plasmatic content was quantified by ELISA to confirm the alterations in biological rhythms, and PER1 and PER2 immunoreactivities were analyzed in brain sections by immunohistochemistry. Results: In the SCN, intracerebroventricular LPS changed PER1 expression, increasing the number of PER1-immunoreactive (IR) cells at zeitgeber time (ZT) 15, decreasing it at ZT5 and ZT20 and not changing it at ZT10. LPS also induced a decrease in PER2-IR cells at ZT5, ZT10, and ZT15 but not at ZT20 in the SCN. In the hippocampus, LPS induced a decrease in PER1-IR and PER2-IR cells at both ZTs (ZT10 and ZT15). In the cerebellum, LPS increased the number of PER1-IR cells at ZT10 and decreased it at ZT15, while the number of PER2-IR cells was reduced at both ZTs. Conclusions: These results indicate that a neuroinflammatory condition leads to desynchronization of primary and subordinate brain oscillators, supporting the existence of the integration between the immune and the circadian system.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP)University of Marilia (UNIMAR) Câmpus Universitário, Av. Higino Muzi Filho 1001University of São Paulo (USP)São Paulo State University (UNESP)FAPESP: 2011/51495-4FAPESP: 2017/23539-3Universidade Estadual Paulista (Unesp)Câmpus UniversitárioUniversidade de São Paulo (USP)Campos, Leila Maria Guissoni [UNESP]Buchaim, Rogerio LeoneDa Silva, Nathani Cristina [UNESP]Spilla, Caio Sergio GalinaHataka, Alessandre [UNESP]Pinato, Luciana [UNESP]2018-12-11T17:35:23Z2018-12-11T17:35:23Z2018-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article231-241application/pdfhttp://dx.doi.org/10.1159/000484931NeuroImmunoModulation, v. 24, n. 4-5, p. 231-241, 2018.1423-02161021-7401http://hdl.handle.net/11449/17948810.1159/0004849312-s2.0-850400568042-s2.0-85040056804.pdf8372363591179624Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNeuroImmunoModulation1,0641,064info:eu-repo/semantics/openAccess2023-10-03T06:02:27Zoai:repositorio.unesp.br:11449/179488Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-03T06:02:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Suprachiasmatic Nucleus and Subordinate Brain Oscillators: Clock Gene Desynchronization by Neuroinflammation |
title |
Suprachiasmatic Nucleus and Subordinate Brain Oscillators: Clock Gene Desynchronization by Neuroinflammation |
spellingShingle |
Suprachiasmatic Nucleus and Subordinate Brain Oscillators: Clock Gene Desynchronization by Neuroinflammation Campos, Leila Maria Guissoni [UNESP] Circadian rhythms Clock genes Neuroinflammation Oscillators |
title_short |
Suprachiasmatic Nucleus and Subordinate Brain Oscillators: Clock Gene Desynchronization by Neuroinflammation |
title_full |
Suprachiasmatic Nucleus and Subordinate Brain Oscillators: Clock Gene Desynchronization by Neuroinflammation |
title_fullStr |
Suprachiasmatic Nucleus and Subordinate Brain Oscillators: Clock Gene Desynchronization by Neuroinflammation |
title_full_unstemmed |
Suprachiasmatic Nucleus and Subordinate Brain Oscillators: Clock Gene Desynchronization by Neuroinflammation |
title_sort |
Suprachiasmatic Nucleus and Subordinate Brain Oscillators: Clock Gene Desynchronization by Neuroinflammation |
author |
Campos, Leila Maria Guissoni [UNESP] |
author_facet |
Campos, Leila Maria Guissoni [UNESP] Buchaim, Rogerio Leone Da Silva, Nathani Cristina [UNESP] Spilla, Caio Sergio Galina Hataka, Alessandre [UNESP] Pinato, Luciana [UNESP] |
author_role |
author |
author2 |
Buchaim, Rogerio Leone Da Silva, Nathani Cristina [UNESP] Spilla, Caio Sergio Galina Hataka, Alessandre [UNESP] Pinato, Luciana [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Câmpus Universitário Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Campos, Leila Maria Guissoni [UNESP] Buchaim, Rogerio Leone Da Silva, Nathani Cristina [UNESP] Spilla, Caio Sergio Galina Hataka, Alessandre [UNESP] Pinato, Luciana [UNESP] |
dc.subject.por.fl_str_mv |
Circadian rhythms Clock genes Neuroinflammation Oscillators |
topic |
Circadian rhythms Clock genes Neuroinflammation Oscillators |
description |
Objective: The clock genes Period (per) 1 and 2 are essential components in the generation and adjustment of biological circadian rhythms by the suprachiasmatic nucleus (SCN). Both genes are also rhythmically present in extrahypothalamic areas such as the hippocampus and cerebellum, considered subordinate oscillators. Several pathological conditions alter rhythmic biological phenomena, but the mechanisms behind these changes involving the clock genes are not well defined. The current study investigated changes in PER1 and PER2 immunoreactivity in the SCN, hippocampus, and cerebellum in a neuroinflammation model. Methods: Wistar rats received lipopolysaccharide (LPS) or vehicle intracerebroventricularly. The melatonin plasmatic content was quantified by ELISA to confirm the alterations in biological rhythms, and PER1 and PER2 immunoreactivities were analyzed in brain sections by immunohistochemistry. Results: In the SCN, intracerebroventricular LPS changed PER1 expression, increasing the number of PER1-immunoreactive (IR) cells at zeitgeber time (ZT) 15, decreasing it at ZT5 and ZT20 and not changing it at ZT10. LPS also induced a decrease in PER2-IR cells at ZT5, ZT10, and ZT15 but not at ZT20 in the SCN. In the hippocampus, LPS induced a decrease in PER1-IR and PER2-IR cells at both ZTs (ZT10 and ZT15). In the cerebellum, LPS increased the number of PER1-IR cells at ZT10 and decreased it at ZT15, while the number of PER2-IR cells was reduced at both ZTs. Conclusions: These results indicate that a neuroinflammatory condition leads to desynchronization of primary and subordinate brain oscillators, supporting the existence of the integration between the immune and the circadian system. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:35:23Z 2018-12-11T17:35:23Z 2018-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1159/000484931 NeuroImmunoModulation, v. 24, n. 4-5, p. 231-241, 2018. 1423-0216 1021-7401 http://hdl.handle.net/11449/179488 10.1159/000484931 2-s2.0-85040056804 2-s2.0-85040056804.pdf 8372363591179624 |
url |
http://dx.doi.org/10.1159/000484931 http://hdl.handle.net/11449/179488 |
identifier_str_mv |
NeuroImmunoModulation, v. 24, n. 4-5, p. 231-241, 2018. 1423-0216 1021-7401 10.1159/000484931 2-s2.0-85040056804 2-s2.0-85040056804.pdf 8372363591179624 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
NeuroImmunoModulation 1,064 1,064 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
231-241 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964412874850304 |