Circadian clock proteins and melatonin receptors in neurons and glia of the Sapajus apella cerebellum

Detalhes bibliográficos
Autor(a) principal: Campos, Leila M. Guissoni [UNESP]
Data de Publicação: 2018
Outros Autores: Hataka, Alessandre [UNESP], Vieira, Isis Z. [UNESP], Buchaim, Rogério L., Robalinho, Isadora F., Arantes, Giovanna E.P.S., Viégas, Joyce S., Bosso, Henrique, Bravos, Rafael M., Pinato, Luciana [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fphys.2018.00005
http://hdl.handle.net/11449/179578
Resumo: Oscillations of brain proteins in circadian rhythms are important for determining several cellular and physiological processes in anticipation of daily and seasonal environmental rhythms. In addition to the suprachiasmatic nucleus, the primary central oscillator, the cerebellum shows oscillations in gene and protein expression. The variety of local circuit rhythms that the cerebellar cortex contains influences functions such as motivational processes, regulation of feeding, food anticipation, language, and working memory. The molecular basis of the cerebellar oscillator has been demonstrated by clock gene expression within cells of the cerebellar layers. Genetic and epidemiological evidence suggests that disruption of circadian rhythms in humans can lead to many pathological conditions. Despite this importance, data about clock gene and protein expression in the cerebellum of diurnal (day-active) species, specifically primates, is currently poorly explored, mainly in regard to cellular identity, as well as the relationship with other molecules also involved in cerebellar functions. These studies could contribute to clarification of the possible mechanisms behind cerebellar rhythmicity. Considering that calcium binding proteins (CaBPs) play crucial roles in preserving and modulating cerebellar functions and that clock gene expression can be controlled by afferent projections or paracrine circadian signals such as the hormone melatonin, the present study aimed to describe cellular identities, distribution patterns and day/night expression changes in PER1, PER2, CaBPs, and MT1 and MT2 melatonin receptors in the cerebellar cortex of a diurnal primate using conventional fluorescence and peroxidase-antiperoxidase immunocytochemical techniques. PER1 and PER2 immunoreactive (IR) cells were observed in the Purkinje cells of the cerebellum, and MT1 and MT2 receptors were localized around Purkinje cells in the Pj layer in Bergmann cells. This identity was confirmed by the S100β-IR of these cells. The highest expression of PER seen in the daytime analysis coincided with the highest expression of melatonin receptors. CaBPs showed day/night morphological and density changes in the cerebellar cortex. The presence of the same temporal variations in the expression of PER in the Pj neurons and in MT1 and MT2 receptors in Bergmann cells indicates a possible relation between these cells during the rhythmic processing of the cerebellum, in addition to the CaBP temporal morphological and density changes.
id UNSP_8ac5d154d5c19d1d63594005f5a1ffb6
oai_identifier_str oai:repositorio.unesp.br:11449/179578
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Circadian clock proteins and melatonin receptors in neurons and glia of the Sapajus apella cerebellumBiological rhythmsCerebellumClock genesCognitionLanguageMelatoninMemoryMotorOscillations of brain proteins in circadian rhythms are important for determining several cellular and physiological processes in anticipation of daily and seasonal environmental rhythms. In addition to the suprachiasmatic nucleus, the primary central oscillator, the cerebellum shows oscillations in gene and protein expression. The variety of local circuit rhythms that the cerebellar cortex contains influences functions such as motivational processes, regulation of feeding, food anticipation, language, and working memory. The molecular basis of the cerebellar oscillator has been demonstrated by clock gene expression within cells of the cerebellar layers. Genetic and epidemiological evidence suggests that disruption of circadian rhythms in humans can lead to many pathological conditions. Despite this importance, data about clock gene and protein expression in the cerebellum of diurnal (day-active) species, specifically primates, is currently poorly explored, mainly in regard to cellular identity, as well as the relationship with other molecules also involved in cerebellar functions. These studies could contribute to clarification of the possible mechanisms behind cerebellar rhythmicity. Considering that calcium binding proteins (CaBPs) play crucial roles in preserving and modulating cerebellar functions and that clock gene expression can be controlled by afferent projections or paracrine circadian signals such as the hormone melatonin, the present study aimed to describe cellular identities, distribution patterns and day/night expression changes in PER1, PER2, CaBPs, and MT1 and MT2 melatonin receptors in the cerebellar cortex of a diurnal primate using conventional fluorescence and peroxidase-antiperoxidase immunocytochemical techniques. PER1 and PER2 immunoreactive (IR) cells were observed in the Purkinje cells of the cerebellum, and MT1 and MT2 receptors were localized around Purkinje cells in the Pj layer in Bergmann cells. This identity was confirmed by the S100β-IR of these cells. The highest expression of PER seen in the daytime analysis coincided with the highest expression of melatonin receptors. CaBPs showed day/night morphological and density changes in the cerebellar cortex. The presence of the same temporal variations in the expression of PER in the Pj neurons and in MT1 and MT2 receptors in Bergmann cells indicates a possible relation between these cells during the rhythmic processing of the cerebellum, in addition to the CaBP temporal morphological and density changes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP)University of Marília (UNIMAR)São Paulo State University - UNESPUniversity of São Paulo - USPSão Paulo State University (UNESP)São Paulo State University - UNESPFAPESP: 2011/51495-4Universidade Estadual Paulista (Unesp)University of Marília (UNIMAR)Universidade de São Paulo (USP)Campos, Leila M. Guissoni [UNESP]Hataka, Alessandre [UNESP]Vieira, Isis Z. [UNESP]Buchaim, Rogério L.Robalinho, Isadora F.Arantes, Giovanna E.P.S.Viégas, Joyce S.Bosso, HenriqueBravos, Rafael M.Pinato, Luciana [UNESP]2018-12-11T17:35:52Z2018-12-11T17:35:52Z2018-02-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.3389/fphys.2018.00005Frontiers in Physiology, v. 9, n. FEB, 2018.1664-042Xhttp://hdl.handle.net/11449/17957810.3389/fphys.2018.000052-s2.0-850418333122-s2.0-85041833312.pdf8372363591179624Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Physiologyinfo:eu-repo/semantics/openAccess2023-11-16T06:09:20Zoai:repositorio.unesp.br:11449/179578Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-16T06:09:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Circadian clock proteins and melatonin receptors in neurons and glia of the Sapajus apella cerebellum
title Circadian clock proteins and melatonin receptors in neurons and glia of the Sapajus apella cerebellum
spellingShingle Circadian clock proteins and melatonin receptors in neurons and glia of the Sapajus apella cerebellum
Campos, Leila M. Guissoni [UNESP]
Biological rhythms
Cerebellum
Clock genes
Cognition
Language
Melatonin
Memory
Motor
title_short Circadian clock proteins and melatonin receptors in neurons and glia of the Sapajus apella cerebellum
title_full Circadian clock proteins and melatonin receptors in neurons and glia of the Sapajus apella cerebellum
title_fullStr Circadian clock proteins and melatonin receptors in neurons and glia of the Sapajus apella cerebellum
title_full_unstemmed Circadian clock proteins and melatonin receptors in neurons and glia of the Sapajus apella cerebellum
title_sort Circadian clock proteins and melatonin receptors in neurons and glia of the Sapajus apella cerebellum
author Campos, Leila M. Guissoni [UNESP]
author_facet Campos, Leila M. Guissoni [UNESP]
Hataka, Alessandre [UNESP]
Vieira, Isis Z. [UNESP]
Buchaim, Rogério L.
Robalinho, Isadora F.
Arantes, Giovanna E.P.S.
Viégas, Joyce S.
Bosso, Henrique
Bravos, Rafael M.
Pinato, Luciana [UNESP]
author_role author
author2 Hataka, Alessandre [UNESP]
Vieira, Isis Z. [UNESP]
Buchaim, Rogério L.
Robalinho, Isadora F.
Arantes, Giovanna E.P.S.
Viégas, Joyce S.
Bosso, Henrique
Bravos, Rafael M.
Pinato, Luciana [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
University of Marília (UNIMAR)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Campos, Leila M. Guissoni [UNESP]
Hataka, Alessandre [UNESP]
Vieira, Isis Z. [UNESP]
Buchaim, Rogério L.
Robalinho, Isadora F.
Arantes, Giovanna E.P.S.
Viégas, Joyce S.
Bosso, Henrique
Bravos, Rafael M.
Pinato, Luciana [UNESP]
dc.subject.por.fl_str_mv Biological rhythms
Cerebellum
Clock genes
Cognition
Language
Melatonin
Memory
Motor
topic Biological rhythms
Cerebellum
Clock genes
Cognition
Language
Melatonin
Memory
Motor
description Oscillations of brain proteins in circadian rhythms are important for determining several cellular and physiological processes in anticipation of daily and seasonal environmental rhythms. In addition to the suprachiasmatic nucleus, the primary central oscillator, the cerebellum shows oscillations in gene and protein expression. The variety of local circuit rhythms that the cerebellar cortex contains influences functions such as motivational processes, regulation of feeding, food anticipation, language, and working memory. The molecular basis of the cerebellar oscillator has been demonstrated by clock gene expression within cells of the cerebellar layers. Genetic and epidemiological evidence suggests that disruption of circadian rhythms in humans can lead to many pathological conditions. Despite this importance, data about clock gene and protein expression in the cerebellum of diurnal (day-active) species, specifically primates, is currently poorly explored, mainly in regard to cellular identity, as well as the relationship with other molecules also involved in cerebellar functions. These studies could contribute to clarification of the possible mechanisms behind cerebellar rhythmicity. Considering that calcium binding proteins (CaBPs) play crucial roles in preserving and modulating cerebellar functions and that clock gene expression can be controlled by afferent projections or paracrine circadian signals such as the hormone melatonin, the present study aimed to describe cellular identities, distribution patterns and day/night expression changes in PER1, PER2, CaBPs, and MT1 and MT2 melatonin receptors in the cerebellar cortex of a diurnal primate using conventional fluorescence and peroxidase-antiperoxidase immunocytochemical techniques. PER1 and PER2 immunoreactive (IR) cells were observed in the Purkinje cells of the cerebellum, and MT1 and MT2 receptors were localized around Purkinje cells in the Pj layer in Bergmann cells. This identity was confirmed by the S100β-IR of these cells. The highest expression of PER seen in the daytime analysis coincided with the highest expression of melatonin receptors. CaBPs showed day/night morphological and density changes in the cerebellar cortex. The presence of the same temporal variations in the expression of PER in the Pj neurons and in MT1 and MT2 receptors in Bergmann cells indicates a possible relation between these cells during the rhythmic processing of the cerebellum, in addition to the CaBP temporal morphological and density changes.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:35:52Z
2018-12-11T17:35:52Z
2018-02-09
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fphys.2018.00005
Frontiers in Physiology, v. 9, n. FEB, 2018.
1664-042X
http://hdl.handle.net/11449/179578
10.3389/fphys.2018.00005
2-s2.0-85041833312
2-s2.0-85041833312.pdf
8372363591179624
url http://dx.doi.org/10.3389/fphys.2018.00005
http://hdl.handle.net/11449/179578
identifier_str_mv Frontiers in Physiology, v. 9, n. FEB, 2018.
1664-042X
10.3389/fphys.2018.00005
2-s2.0-85041833312
2-s2.0-85041833312.pdf
8372363591179624
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Physiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799964953233326080