Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática

Detalhes bibliográficos
Autor(a) principal: Sossella, Francine de Souza
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF)
Texto Completo: http://tede.upf.br:8080/jspui/handle/tede/1805
Resumo: Microalgae are an alternative to fossil fuels, as they can be applied in the production of biofuels. The environmental and technical advantages are proven, showing that the productivity of microalgae, when compared to traditional crops (corn and soybean, for example), is higher. However, one of the problems in production is the high cost and the harvesting of biomass is one of the most costly stages. In this way, finding more suitable and viable methods becomes necessary. One of the indicated processes for separation is the use of coagulants. In the case of ethanol production, which involves enzymatic and fermentative processes, many factors can affect the conversion efficiency into product. Therefore, the objective of this work was to study the separation of Spirulina platensis with different coagulants and their influence on enzymatic hydrolysis processes. Cultures of Spirulina platensis LEB 52 were performed in raceways, in semi-continuous mode. The microalgae harvest was carried out with 11 types of coagulants, these being organic and inorganic, and a comparison with the centrifugation method. For the determination of the best harvesting condition, central compound designs were performed, varying two factors (pH and concentration) at two levels. With the ideal harvest conditions for each coagulant, it followed the application on a larger scale, characterization of the biomass was carried out regarding the carbohydrate, protein, ash, moisture content and SEM/EDS analysis. The biomass was broken by the freezing and thawing method followed by enzymatic hydrolysis of carbohydrates and proteins. The coagulants were efficient in separating the biomass, reaching efficiencies up to 99.5%. In the chemical characterization, high ash content was identified from the culture medium salts and retained coagulant. Ferric aluminum sulphate stood out in saccharification, converting approximately 37.52% of the carbohydrates into reducing sugar. In protein hydrolysis, low hydrolysis efficiencies were obtained (1.26 - 4.07%). The coagulation and flocculation process has a promising application area, for ease of large-scale application and high removal efficiencies. However, for use in biotechnological processes studies are suggested to separate biomass coagulants previously.
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spelling Colla, Luciane Maria02309369977http://lattes.cnpq.br/480430403645564002923382013http://lattes.cnpq.br/7614095462292601Sossella, Francine de Souza2019-10-30T19:31:39Z2019-04-12SOSSELLA, Francine de Souza. Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática. 2019. 74 f. Dissertação (Mestrado em Engenharia Civil e Ambiental) - Universidade de Passo Fundo, Passo Fundo, RS, 2019.http://tede.upf.br:8080/jspui/handle/tede/1805Microalgae are an alternative to fossil fuels, as they can be applied in the production of biofuels. The environmental and technical advantages are proven, showing that the productivity of microalgae, when compared to traditional crops (corn and soybean, for example), is higher. However, one of the problems in production is the high cost and the harvesting of biomass is one of the most costly stages. In this way, finding more suitable and viable methods becomes necessary. One of the indicated processes for separation is the use of coagulants. In the case of ethanol production, which involves enzymatic and fermentative processes, many factors can affect the conversion efficiency into product. Therefore, the objective of this work was to study the separation of Spirulina platensis with different coagulants and their influence on enzymatic hydrolysis processes. Cultures of Spirulina platensis LEB 52 were performed in raceways, in semi-continuous mode. The microalgae harvest was carried out with 11 types of coagulants, these being organic and inorganic, and a comparison with the centrifugation method. For the determination of the best harvesting condition, central compound designs were performed, varying two factors (pH and concentration) at two levels. With the ideal harvest conditions for each coagulant, it followed the application on a larger scale, characterization of the biomass was carried out regarding the carbohydrate, protein, ash, moisture content and SEM/EDS analysis. The biomass was broken by the freezing and thawing method followed by enzymatic hydrolysis of carbohydrates and proteins. The coagulants were efficient in separating the biomass, reaching efficiencies up to 99.5%. In the chemical characterization, high ash content was identified from the culture medium salts and retained coagulant. Ferric aluminum sulphate stood out in saccharification, converting approximately 37.52% of the carbohydrates into reducing sugar. In protein hydrolysis, low hydrolysis efficiencies were obtained (1.26 - 4.07%). The coagulation and flocculation process has a promising application area, for ease of large-scale application and high removal efficiencies. However, for use in biotechnological processes studies are suggested to separate biomass coagulants previously.As microalgas são apontadas como uma alternativa aos combustíveis fósseis, pois podem ser aplicadas na produção de biocombustíveis. As vantagens ambientais e técnicas já foram comprovadas, mostrando que a produtividade de cultivos de microalgas, quando comparadas com as culturas tradicionais (milho e soja, por exemplo), é superior. Entretanto, um dos problemas na produção é o elevado custo e a colheita da biomassa figura como uma das etapas mais onerosas. Desta forma, buscar métodos mais adequados e viáveis se torna necessário. Um dos processos indicados para separação é o uso de coagulantes. No caso da produção de etanol, que envolve processos enzimáticos e fermentativos, muitos fatores podem afetar a eficiência de conversão em produto. Com isso, o objetivo do trabalho foi estudar a separação da Spirulina platensis com diferentes coagulantes e as influências destes nos processos de hidrólise enzimática. Foram realizados cultivos de Spirulina platensis LEB 52 em tanques abertos, em modo semi-contínuo. A colheita da microalga foi realizada com 11 tipos de coagulantes, sendo estes orgânicos e inorgânicos, e uma comparação com o método de centrifugação. Para a determinação da melhor condição de colheita, foram realizados delineamentos compostos centrais, variando dois fatores (pH e concentração) em dois níveis. Com as condições de colheita ideais para cada coagulante, seguiu a aplicação em maior escala, caracterização da biomassa foi realizada quanto a teor de carboidratos, proteínas, cinzas, umidade e análise em MEV/EDS. A biomassa foi rompida pelo método de congelamento e descongelamento seguida de hidrólise enzimática dos carboidratos e das proteínas. Os coagulantes se mostraram eficientes na separação da biomassa, alcançando eficiências de até 99,5%. Na caracterização química foi identificado alto teor de cinzas, proveniente dos sais do meio de cultivo e coagulante retido. O sulfato de alumínio férrico se destacou na sacarificação, convertendo aproximadamente 37,52% dos carboidratos em açúcar redutor. Na hidrólise proteica, baixas eficiências de hidrólise foram obtidas (1,26 - 4,07 %). O processo de coagulação e floculação tem um campo de aplicação promissor, devida à facilidade de aplicação em cultivos de grande escala e as altas eficiências de remoção. No entanto, para utilização em processos biotecnológicos sugerem-se estudos para separação dos coagulantes da biomassa previamente.Submitted by Aline Rezende (alinerezende@upf.br) on 2019-10-30T19:31:39Z No. of bitstreams: 1 2019FrancineSouzaSossella.pdf: 2227633 bytes, checksum: da23703d730c1b74e15cc3577664c4c2 (MD5)Made available in DSpace on 2019-10-30T19:31:39Z (GMT). No. of bitstreams: 1 2019FrancineSouzaSossella.pdf: 2227633 bytes, checksum: da23703d730c1b74e15cc3577664c4c2 (MD5) Previous issue date: 2019-04-12application/pdfporUniversidade de Passo FundoPrograma de Pós-Graduação em Engenharia Civil e AmbientalUPFBrasilFaculdade de Engenharia e Arquitetura – FEARAlgaBiocombustíveisEnergia da biomassaSpirulinaENGENHARIAS::ENGENHARIA CIVILEfeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimáticaCoagulation effects on S. platensis biomass hydrolysis harvest on enzymatic hydrolysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-41729612957170071185005006008147033241558623806-6274833215046395772info:eu-repo/semantics/openAccessreponame:Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF)instname:Universidade de Passo Fundo (UPF)instacron:UPFORIGINAL2019FrancineSouzaSossella.pdf2019FrancineSouzaSossella.pdfapplication/pdf2227633http://tede.upf.br:8080/jspui/bitstream/tede/1805/2/2019FrancineSouzaSossella.pdfda23703d730c1b74e15cc3577664c4c2MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede.upf.br:8080/jspui/bitstream/tede/1805/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/18052019-10-30 16:31:39.376oai:tede.upf.br: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Biblioteca Digital de Teses e DissertaçõesPUBhttp://tede.upf.br/oai/requestbiblio@upf.br || bio@upf.br || cas@upf.br || car@upf.br || lve@upf.br || sar@upf.br || sol@upf.br || upfmundi@upf.br || jucelei@upf.bropendoar:2019-10-30T19:31:39Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) - Universidade de Passo Fundo (UPF)false
dc.title.por.fl_str_mv Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática
dc.title.alternative.eng.fl_str_mv Coagulation effects on S. platensis biomass hydrolysis harvest on enzymatic hydrolysis
title Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática
spellingShingle Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática
Sossella, Francine de Souza
Alga
Biocombustíveis
Energia da biomassa
Spirulina
ENGENHARIAS::ENGENHARIA CIVIL
title_short Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática
title_full Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática
title_fullStr Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática
title_full_unstemmed Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática
title_sort Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática
author Sossella, Francine de Souza
author_facet Sossella, Francine de Souza
author_role author
dc.contributor.advisor1.fl_str_mv Colla, Luciane Maria
dc.contributor.advisor1ID.fl_str_mv 02309369977
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4804304036455640
dc.contributor.authorID.fl_str_mv 02923382013
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7614095462292601
dc.contributor.author.fl_str_mv Sossella, Francine de Souza
contributor_str_mv Colla, Luciane Maria
dc.subject.por.fl_str_mv Alga
Biocombustíveis
Energia da biomassa
Spirulina
topic Alga
Biocombustíveis
Energia da biomassa
Spirulina
ENGENHARIAS::ENGENHARIA CIVIL
dc.subject.cnpq.fl_str_mv ENGENHARIAS::ENGENHARIA CIVIL
description Microalgae are an alternative to fossil fuels, as they can be applied in the production of biofuels. The environmental and technical advantages are proven, showing that the productivity of microalgae, when compared to traditional crops (corn and soybean, for example), is higher. However, one of the problems in production is the high cost and the harvesting of biomass is one of the most costly stages. In this way, finding more suitable and viable methods becomes necessary. One of the indicated processes for separation is the use of coagulants. In the case of ethanol production, which involves enzymatic and fermentative processes, many factors can affect the conversion efficiency into product. Therefore, the objective of this work was to study the separation of Spirulina platensis with different coagulants and their influence on enzymatic hydrolysis processes. Cultures of Spirulina platensis LEB 52 were performed in raceways, in semi-continuous mode. The microalgae harvest was carried out with 11 types of coagulants, these being organic and inorganic, and a comparison with the centrifugation method. For the determination of the best harvesting condition, central compound designs were performed, varying two factors (pH and concentration) at two levels. With the ideal harvest conditions for each coagulant, it followed the application on a larger scale, characterization of the biomass was carried out regarding the carbohydrate, protein, ash, moisture content and SEM/EDS analysis. The biomass was broken by the freezing and thawing method followed by enzymatic hydrolysis of carbohydrates and proteins. The coagulants were efficient in separating the biomass, reaching efficiencies up to 99.5%. In the chemical characterization, high ash content was identified from the culture medium salts and retained coagulant. Ferric aluminum sulphate stood out in saccharification, converting approximately 37.52% of the carbohydrates into reducing sugar. In protein hydrolysis, low hydrolysis efficiencies were obtained (1.26 - 4.07%). The coagulation and flocculation process has a promising application area, for ease of large-scale application and high removal efficiencies. However, for use in biotechnological processes studies are suggested to separate biomass coagulants previously.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-10-30T19:31:39Z
dc.date.issued.fl_str_mv 2019-04-12
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dc.identifier.citation.fl_str_mv SOSSELLA, Francine de Souza. Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática. 2019. 74 f. Dissertação (Mestrado em Engenharia Civil e Ambiental) - Universidade de Passo Fundo, Passo Fundo, RS, 2019.
dc.identifier.uri.fl_str_mv http://tede.upf.br:8080/jspui/handle/tede/1805
identifier_str_mv SOSSELLA, Francine de Souza. Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática. 2019. 74 f. Dissertação (Mestrado em Engenharia Civil e Ambiental) - Universidade de Passo Fundo, Passo Fundo, RS, 2019.
url http://tede.upf.br:8080/jspui/handle/tede/1805
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