Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) |
Texto Completo: | http://tede.upf.br:8080/jspui/handle/tede/1805 |
Resumo: | Microalgae are an alternative to fossil fuels, as they can be applied in the production of biofuels. The environmental and technical advantages are proven, showing that the productivity of microalgae, when compared to traditional crops (corn and soybean, for example), is higher. However, one of the problems in production is the high cost and the harvesting of biomass is one of the most costly stages. In this way, finding more suitable and viable methods becomes necessary. One of the indicated processes for separation is the use of coagulants. In the case of ethanol production, which involves enzymatic and fermentative processes, many factors can affect the conversion efficiency into product. Therefore, the objective of this work was to study the separation of Spirulina platensis with different coagulants and their influence on enzymatic hydrolysis processes. Cultures of Spirulina platensis LEB 52 were performed in raceways, in semi-continuous mode. The microalgae harvest was carried out with 11 types of coagulants, these being organic and inorganic, and a comparison with the centrifugation method. For the determination of the best harvesting condition, central compound designs were performed, varying two factors (pH and concentration) at two levels. With the ideal harvest conditions for each coagulant, it followed the application on a larger scale, characterization of the biomass was carried out regarding the carbohydrate, protein, ash, moisture content and SEM/EDS analysis. The biomass was broken by the freezing and thawing method followed by enzymatic hydrolysis of carbohydrates and proteins. The coagulants were efficient in separating the biomass, reaching efficiencies up to 99.5%. In the chemical characterization, high ash content was identified from the culture medium salts and retained coagulant. Ferric aluminum sulphate stood out in saccharification, converting approximately 37.52% of the carbohydrates into reducing sugar. In protein hydrolysis, low hydrolysis efficiencies were obtained (1.26 - 4.07%). The coagulation and flocculation process has a promising application area, for ease of large-scale application and high removal efficiencies. However, for use in biotechnological processes studies are suggested to separate biomass coagulants previously. |
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Colla, Luciane Maria02309369977http://lattes.cnpq.br/480430403645564002923382013http://lattes.cnpq.br/7614095462292601Sossella, Francine de Souza2019-10-30T19:31:39Z2019-04-12SOSSELLA, Francine de Souza. Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática. 2019. 74 f. Dissertação (Mestrado em Engenharia Civil e Ambiental) - Universidade de Passo Fundo, Passo Fundo, RS, 2019.http://tede.upf.br:8080/jspui/handle/tede/1805Microalgae are an alternative to fossil fuels, as they can be applied in the production of biofuels. The environmental and technical advantages are proven, showing that the productivity of microalgae, when compared to traditional crops (corn and soybean, for example), is higher. However, one of the problems in production is the high cost and the harvesting of biomass is one of the most costly stages. In this way, finding more suitable and viable methods becomes necessary. One of the indicated processes for separation is the use of coagulants. In the case of ethanol production, which involves enzymatic and fermentative processes, many factors can affect the conversion efficiency into product. Therefore, the objective of this work was to study the separation of Spirulina platensis with different coagulants and their influence on enzymatic hydrolysis processes. Cultures of Spirulina platensis LEB 52 were performed in raceways, in semi-continuous mode. The microalgae harvest was carried out with 11 types of coagulants, these being organic and inorganic, and a comparison with the centrifugation method. For the determination of the best harvesting condition, central compound designs were performed, varying two factors (pH and concentration) at two levels. With the ideal harvest conditions for each coagulant, it followed the application on a larger scale, characterization of the biomass was carried out regarding the carbohydrate, protein, ash, moisture content and SEM/EDS analysis. The biomass was broken by the freezing and thawing method followed by enzymatic hydrolysis of carbohydrates and proteins. The coagulants were efficient in separating the biomass, reaching efficiencies up to 99.5%. In the chemical characterization, high ash content was identified from the culture medium salts and retained coagulant. Ferric aluminum sulphate stood out in saccharification, converting approximately 37.52% of the carbohydrates into reducing sugar. In protein hydrolysis, low hydrolysis efficiencies were obtained (1.26 - 4.07%). The coagulation and flocculation process has a promising application area, for ease of large-scale application and high removal efficiencies. However, for use in biotechnological processes studies are suggested to separate biomass coagulants previously.As microalgas são apontadas como uma alternativa aos combustíveis fósseis, pois podem ser aplicadas na produção de biocombustíveis. As vantagens ambientais e técnicas já foram comprovadas, mostrando que a produtividade de cultivos de microalgas, quando comparadas com as culturas tradicionais (milho e soja, por exemplo), é superior. Entretanto, um dos problemas na produção é o elevado custo e a colheita da biomassa figura como uma das etapas mais onerosas. Desta forma, buscar métodos mais adequados e viáveis se torna necessário. Um dos processos indicados para separação é o uso de coagulantes. No caso da produção de etanol, que envolve processos enzimáticos e fermentativos, muitos fatores podem afetar a eficiência de conversão em produto. Com isso, o objetivo do trabalho foi estudar a separação da Spirulina platensis com diferentes coagulantes e as influências destes nos processos de hidrólise enzimática. Foram realizados cultivos de Spirulina platensis LEB 52 em tanques abertos, em modo semi-contínuo. A colheita da microalga foi realizada com 11 tipos de coagulantes, sendo estes orgânicos e inorgânicos, e uma comparação com o método de centrifugação. Para a determinação da melhor condição de colheita, foram realizados delineamentos compostos centrais, variando dois fatores (pH e concentração) em dois níveis. Com as condições de colheita ideais para cada coagulante, seguiu a aplicação em maior escala, caracterização da biomassa foi realizada quanto a teor de carboidratos, proteínas, cinzas, umidade e análise em MEV/EDS. A biomassa foi rompida pelo método de congelamento e descongelamento seguida de hidrólise enzimática dos carboidratos e das proteínas. Os coagulantes se mostraram eficientes na separação da biomassa, alcançando eficiências de até 99,5%. Na caracterização química foi identificado alto teor de cinzas, proveniente dos sais do meio de cultivo e coagulante retido. O sulfato de alumínio férrico se destacou na sacarificação, convertendo aproximadamente 37,52% dos carboidratos em açúcar redutor. Na hidrólise proteica, baixas eficiências de hidrólise foram obtidas (1,26 - 4,07 %). O processo de coagulação e floculação tem um campo de aplicação promissor, devida à facilidade de aplicação em cultivos de grande escala e as altas eficiências de remoção. No entanto, para utilização em processos biotecnológicos sugerem-se estudos para separação dos coagulantes da biomassa previamente.Submitted by Aline Rezende (alinerezende@upf.br) on 2019-10-30T19:31:39Z No. of bitstreams: 1 2019FrancineSouzaSossella.pdf: 2227633 bytes, checksum: da23703d730c1b74e15cc3577664c4c2 (MD5)Made available in DSpace on 2019-10-30T19:31:39Z (GMT). No. of bitstreams: 1 2019FrancineSouzaSossella.pdf: 2227633 bytes, checksum: da23703d730c1b74e15cc3577664c4c2 (MD5) Previous issue date: 2019-04-12application/pdfporUniversidade de Passo FundoPrograma de Pós-Graduação em Engenharia Civil e AmbientalUPFBrasilFaculdade de Engenharia e Arquitetura – FEARAlgaBiocombustíveisEnergia da biomassaSpirulinaENGENHARIAS::ENGENHARIA CIVILEfeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimáticaCoagulation effects on S. platensis biomass hydrolysis harvest on enzymatic hydrolysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-41729612957170071185005006008147033241558623806-6274833215046395772info:eu-repo/semantics/openAccessreponame:Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF)instname:Universidade de Passo Fundo (UPF)instacron:UPFORIGINAL2019FrancineSouzaSossella.pdf2019FrancineSouzaSossella.pdfapplication/pdf2227633http://tede.upf.br:8080/jspui/bitstream/tede/1805/2/2019FrancineSouzaSossella.pdfda23703d730c1b74e15cc3577664c4c2MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede.upf.br:8080/jspui/bitstream/tede/1805/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/18052019-10-30 16:31:39.376oai:tede.upf.br: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Biblioteca Digital de Teses e DissertaçõesPUBhttp://tede.upf.br/oai/requestbiblio@upf.br || bio@upf.br || cas@upf.br || car@upf.br || lve@upf.br || sar@upf.br || sol@upf.br || upfmundi@upf.br || jucelei@upf.bropendoar:2019-10-30T19:31:39Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) - Universidade de Passo Fundo (UPF)false |
dc.title.por.fl_str_mv |
Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática |
dc.title.alternative.eng.fl_str_mv |
Coagulation effects on S. platensis biomass hydrolysis harvest on enzymatic hydrolysis |
title |
Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática |
spellingShingle |
Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática Sossella, Francine de Souza Alga Biocombustíveis Energia da biomassa Spirulina ENGENHARIAS::ENGENHARIA CIVIL |
title_short |
Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática |
title_full |
Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática |
title_fullStr |
Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática |
title_full_unstemmed |
Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática |
title_sort |
Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática |
author |
Sossella, Francine de Souza |
author_facet |
Sossella, Francine de Souza |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Colla, Luciane Maria |
dc.contributor.advisor1ID.fl_str_mv |
02309369977 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4804304036455640 |
dc.contributor.authorID.fl_str_mv |
02923382013 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7614095462292601 |
dc.contributor.author.fl_str_mv |
Sossella, Francine de Souza |
contributor_str_mv |
Colla, Luciane Maria |
dc.subject.por.fl_str_mv |
Alga Biocombustíveis Energia da biomassa Spirulina |
topic |
Alga Biocombustíveis Energia da biomassa Spirulina ENGENHARIAS::ENGENHARIA CIVIL |
dc.subject.cnpq.fl_str_mv |
ENGENHARIAS::ENGENHARIA CIVIL |
description |
Microalgae are an alternative to fossil fuels, as they can be applied in the production of biofuels. The environmental and technical advantages are proven, showing that the productivity of microalgae, when compared to traditional crops (corn and soybean, for example), is higher. However, one of the problems in production is the high cost and the harvesting of biomass is one of the most costly stages. In this way, finding more suitable and viable methods becomes necessary. One of the indicated processes for separation is the use of coagulants. In the case of ethanol production, which involves enzymatic and fermentative processes, many factors can affect the conversion efficiency into product. Therefore, the objective of this work was to study the separation of Spirulina platensis with different coagulants and their influence on enzymatic hydrolysis processes. Cultures of Spirulina platensis LEB 52 were performed in raceways, in semi-continuous mode. The microalgae harvest was carried out with 11 types of coagulants, these being organic and inorganic, and a comparison with the centrifugation method. For the determination of the best harvesting condition, central compound designs were performed, varying two factors (pH and concentration) at two levels. With the ideal harvest conditions for each coagulant, it followed the application on a larger scale, characterization of the biomass was carried out regarding the carbohydrate, protein, ash, moisture content and SEM/EDS analysis. The biomass was broken by the freezing and thawing method followed by enzymatic hydrolysis of carbohydrates and proteins. The coagulants were efficient in separating the biomass, reaching efficiencies up to 99.5%. In the chemical characterization, high ash content was identified from the culture medium salts and retained coagulant. Ferric aluminum sulphate stood out in saccharification, converting approximately 37.52% of the carbohydrates into reducing sugar. In protein hydrolysis, low hydrolysis efficiencies were obtained (1.26 - 4.07%). The coagulation and flocculation process has a promising application area, for ease of large-scale application and high removal efficiencies. However, for use in biotechnological processes studies are suggested to separate biomass coagulants previously. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-10-30T19:31:39Z |
dc.date.issued.fl_str_mv |
2019-04-12 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
SOSSELLA, Francine de Souza. Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática. 2019. 74 f. Dissertação (Mestrado em Engenharia Civil e Ambiental) - Universidade de Passo Fundo, Passo Fundo, RS, 2019. |
dc.identifier.uri.fl_str_mv |
http://tede.upf.br:8080/jspui/handle/tede/1805 |
identifier_str_mv |
SOSSELLA, Francine de Souza. Efeitos de coagulação na colheita de hidrólise de biomassa de S. platensis sobre a hidrólise enzimática. 2019. 74 f. Dissertação (Mestrado em Engenharia Civil e Ambiental) - Universidade de Passo Fundo, Passo Fundo, RS, 2019. |
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http://tede.upf.br:8080/jspui/handle/tede/1805 |
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por |
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por |
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500 500 600 |
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UPF |
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Universidade de Passo Fundo |
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