Caracterização citogenética clássica e molecular de trigos brasileiros

Detalhes bibliográficos
Autor(a) principal: Urio, Elisandra Andreia
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF)
Texto Completo: http://10.0.217.128:8080/jspui/handle/tede/554
Resumo: The present study sought to characterize cytogenetically and infer the genetic stability of Brazilian wheat cultivars. The biological material consisted of old and modern cultivars, all kept in the Active Germplasm Bank of Embrapa Wheat, consisting of two distinct groups. In group 1, it was selected 17 cultivars launched after the year 2000, which were evaluated for the presence of micronuclei in tetrads, and the pollen viability. In group 2, it was selected 70 cultivars launched before the year 2000, which were evaluated for pollen viability,. For tetrad analysis, they were classified as normal (absence of micronuclei), triads and micronuclei. To check pollen viability, pollen grains it was classified as viable (two and three nuclei and the presence of starch) and nonviable (empty), by the size and presence of more than one pore. Specifically for group 1, cultivars BR 24, Timbaúva BRS, BRS 208 and BRS 220 were selected by greater variability estimated, determined by Tocher method, aiming the chromosomal characterization through In Situ Hybridization (FISH and GISH). For FISH, probles were used as synthetic oligonucleotides (AAC) 5 and (AAG) 5, present in the three wheat genomes. For GISH, the genomic DNA of rye was used as probe to verify the translocation 1BL.1RS. Data were analyzed by Scott-Knott 5% test. For group 1, the analysis of tetrads found a significant difference in the presence of micronuclei and on the pollen viability, only on the variable size difference. In group 2, it was found nonviable, viable and size difference pollen grains. For FISH analysis, all cultivars showed distinct marking sites for both (AAC) 5 and for (AAG) 5. However, the oligonucleotide (AAC) 5 was more strongly marked in all materials, demonstrating variability among cultivars. For GISH, BRS 208 was the only one that presented the translocation 1BL.1RS. Therefore, the results generated can serve as support in genetic improvement projects, particularly in the incorporation of desirable agronomic characteristics present in the cultivars analyzed, as well as to produce hybrids between T.aestivum and different Triticeae species
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spelling Brammer, Sandra PatussiCPF:88888888888Heinsfeld, AdelarCPF:46922660900http://lattes.cnpq.br/1441337158224362CPF:93360630068http://lattes.cnpq.br/3556778886313983Urio, Elisandra Andreia2018-01-10T18:03:07Z2015-09-032013-05-03URIO, Elisandra Andreia. Caracterização citogenética clássica e molecular de trigos brasileiros. 2013. 123 f. Dissertação (Mestrado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, 2013.http://10.0.217.128:8080/jspui/handle/tede/554The present study sought to characterize cytogenetically and infer the genetic stability of Brazilian wheat cultivars. The biological material consisted of old and modern cultivars, all kept in the Active Germplasm Bank of Embrapa Wheat, consisting of two distinct groups. In group 1, it was selected 17 cultivars launched after the year 2000, which were evaluated for the presence of micronuclei in tetrads, and the pollen viability. In group 2, it was selected 70 cultivars launched before the year 2000, which were evaluated for pollen viability,. For tetrad analysis, they were classified as normal (absence of micronuclei), triads and micronuclei. To check pollen viability, pollen grains it was classified as viable (two and three nuclei and the presence of starch) and nonviable (empty), by the size and presence of more than one pore. Specifically for group 1, cultivars BR 24, Timbaúva BRS, BRS 208 and BRS 220 were selected by greater variability estimated, determined by Tocher method, aiming the chromosomal characterization through In Situ Hybridization (FISH and GISH). For FISH, probles were used as synthetic oligonucleotides (AAC) 5 and (AAG) 5, present in the three wheat genomes. For GISH, the genomic DNA of rye was used as probe to verify the translocation 1BL.1RS. Data were analyzed by Scott-Knott 5% test. For group 1, the analysis of tetrads found a significant difference in the presence of micronuclei and on the pollen viability, only on the variable size difference. In group 2, it was found nonviable, viable and size difference pollen grains. For FISH analysis, all cultivars showed distinct marking sites for both (AAC) 5 and for (AAG) 5. However, the oligonucleotide (AAC) 5 was more strongly marked in all materials, demonstrating variability among cultivars. For GISH, BRS 208 was the only one that presented the translocation 1BL.1RS. Therefore, the results generated can serve as support in genetic improvement projects, particularly in the incorporation of desirable agronomic characteristics present in the cultivars analyzed, as well as to produce hybrids between T.aestivum and different Triticeae speciesO presente estudo teve por finalidade caracterizar citogeneticamente e inferir sobre a estabilidade genética de cultivares de trigos brasileiros. O material biológico foi constituído de cultivares antigas e modernas, todas mantidas no Banco Ativo de Germoplasma da Embrapa Trigo, consistindo de dois grupos distintos. No grupo 1 foram selecionadas 17 cultivares lançadas posteriormente ao ano de 2000, as quais foram avaliadas pela presença de micronúcleos em tétrades e pela viabilidade polínica. No grupo 2, foram selecionadas 70 cultivares, lançadas antes do ano de 2000, avaliadas quanto à viabilidade polínica. Para a análise de tétrades, as mesmas foram classificadas como normais (ausência de micronúcleos), tríades e presença de micronúcleos. Para verificar a viabilidade polínica, os grãos de pólen foram classificados como viáveis (bi e trinucleados e presença de amido) e inviáveis (vazios), pelo tamanho e presença de mais de um poro. Especificamente para o grupo 1, as cultivares BR 24, BRS Timbaúva, BRS 208 e BRS 220 foram selecionadas mediante a estimativa da maior variabilidade, determinadas pelo método de Tocher, visando à caracterização cromossômica via Hibridização In Situ (FISH e GISH). Para a FISH, foram utilizados como sondas os oligonucleotídeos sintéticos (AAC)5 e (AAG)5, presentes nos três genomas do trigo. Para a GISH, utilizou-se a sonda de DNA genômico de centeio para verificar a translocação 1BL.1RS. Os dados foram analisados pelo teste estatístico Scott e Knott 5%. Para o grupo 1, na análise das tétrades constatou-se diferença significativa para a presença de micronúcleos e quanto à viabilidade polínica, apenas para a variável diferença de tamanho. No grupo 2, foi verificado grãos de pólen inviáveis, viáveis e com diferença de tamanho. Para as análises de FISH, todas as cultivares apresentaram sítios de marcação distintos tanto para (AAC)5 como para (AAG)5. Entretanto, o oligonucleotídeo (AAC)5 foi mais fortemente marcado em todos os materiais, demonstrando variabilidade entre as cultivares. Para a GISH, a cultivar BRS 208 apresentou a translocação 1BL.1RS. Portanto, os resultados gerados poderão servir de subsídios em projetos de melhoramento genético, principalmente na incorporação de características de interesse agronômico presentes nas cultivares analisadas, bem como na obtenção de híbridos entre T. aestivum e diferentes espécies de TriticeaeMade available in DSpace on 2018-01-10T18:03:07Z (GMT). No. of bitstreams: 1 2013ElisandraAndreiaUrio.pdf: 2350599 bytes, checksum: 2ebdb6fe4bf065c9670d0d54431ed75f (MD5) Previous issue date: 2013-05-03application/pdfporUniversidade de Passo FundoPrograma de Pós-Graduação em AgronomiaUPFBRFaculdade de Agronomia e Medicina Veterinária – FAMVTrigo - Melhoramento genéticoTrigo - CultivoWheat - BreedingWheat - GrowingCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETALCaracterização citogenética clássica e molecular de trigos brasileirosClassical and molecular cytogenetic characterization of Brazilian wheatsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis321486006006005458779698989info:eu-repo/semantics/openAccessreponame:Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF)instname:Universidade de Passo Fundo (UPF)instacron:UPFORIGINAL2013ElisandraAndreiaUrio.pdfapplication/pdf2350599http://tede.upf.br:8080/jspui/bitstream/tede/554/1/2013ElisandraAndreiaUrio.pdf2ebdb6fe4bf065c9670d0d54431ed75fMD51tede/5542018-09-05 18:41:29.566oai:tede.upf.br:tede/554Biblioteca Digital de Teses e DissertaçõesPUBhttp://tede.upf.br/oai/requestbiblio@upf.br || bio@upf.br || cas@upf.br || car@upf.br || lve@upf.br || sar@upf.br || sol@upf.br || upfmundi@upf.br || jucelei@upf.bropendoar:2018-09-05T21:41:29Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) - Universidade de Passo Fundo (UPF)false
dc.title.por.fl_str_mv Caracterização citogenética clássica e molecular de trigos brasileiros
dc.title.alternative.eng.fl_str_mv Classical and molecular cytogenetic characterization of Brazilian wheats
title Caracterização citogenética clássica e molecular de trigos brasileiros
spellingShingle Caracterização citogenética clássica e molecular de trigos brasileiros
Urio, Elisandra Andreia
Trigo - Melhoramento genético
Trigo - Cultivo
Wheat - Breeding
Wheat - Growing
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL
title_short Caracterização citogenética clássica e molecular de trigos brasileiros
title_full Caracterização citogenética clássica e molecular de trigos brasileiros
title_fullStr Caracterização citogenética clássica e molecular de trigos brasileiros
title_full_unstemmed Caracterização citogenética clássica e molecular de trigos brasileiros
title_sort Caracterização citogenética clássica e molecular de trigos brasileiros
author Urio, Elisandra Andreia
author_facet Urio, Elisandra Andreia
author_role author
dc.contributor.advisor1.fl_str_mv Brammer, Sandra Patussi
dc.contributor.advisor1ID.fl_str_mv CPF:88888888888
dc.contributor.advisor-co1.fl_str_mv Heinsfeld, Adelar
dc.contributor.advisor-co1ID.fl_str_mv CPF:46922660900
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/1441337158224362
dc.contributor.authorID.fl_str_mv CPF:93360630068
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3556778886313983
dc.contributor.author.fl_str_mv Urio, Elisandra Andreia
contributor_str_mv Brammer, Sandra Patussi
Heinsfeld, Adelar
dc.subject.por.fl_str_mv Trigo - Melhoramento genético
Trigo - Cultivo
topic Trigo - Melhoramento genético
Trigo - Cultivo
Wheat - Breeding
Wheat - Growing
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL
dc.subject.eng.fl_str_mv Wheat - Breeding
Wheat - Growing
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL
description The present study sought to characterize cytogenetically and infer the genetic stability of Brazilian wheat cultivars. The biological material consisted of old and modern cultivars, all kept in the Active Germplasm Bank of Embrapa Wheat, consisting of two distinct groups. In group 1, it was selected 17 cultivars launched after the year 2000, which were evaluated for the presence of micronuclei in tetrads, and the pollen viability. In group 2, it was selected 70 cultivars launched before the year 2000, which were evaluated for pollen viability,. For tetrad analysis, they were classified as normal (absence of micronuclei), triads and micronuclei. To check pollen viability, pollen grains it was classified as viable (two and three nuclei and the presence of starch) and nonviable (empty), by the size and presence of more than one pore. Specifically for group 1, cultivars BR 24, Timbaúva BRS, BRS 208 and BRS 220 were selected by greater variability estimated, determined by Tocher method, aiming the chromosomal characterization through In Situ Hybridization (FISH and GISH). For FISH, probles were used as synthetic oligonucleotides (AAC) 5 and (AAG) 5, present in the three wheat genomes. For GISH, the genomic DNA of rye was used as probe to verify the translocation 1BL.1RS. Data were analyzed by Scott-Knott 5% test. For group 1, the analysis of tetrads found a significant difference in the presence of micronuclei and on the pollen viability, only on the variable size difference. In group 2, it was found nonviable, viable and size difference pollen grains. For FISH analysis, all cultivars showed distinct marking sites for both (AAC) 5 and for (AAG) 5. However, the oligonucleotide (AAC) 5 was more strongly marked in all materials, demonstrating variability among cultivars. For GISH, BRS 208 was the only one that presented the translocation 1BL.1RS. Therefore, the results generated can serve as support in genetic improvement projects, particularly in the incorporation of desirable agronomic characteristics present in the cultivars analyzed, as well as to produce hybrids between T.aestivum and different Triticeae species
publishDate 2013
dc.date.issued.fl_str_mv 2013-05-03
dc.date.available.fl_str_mv 2015-09-03
dc.date.accessioned.fl_str_mv 2018-01-10T18:03:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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status_str publishedVersion
dc.identifier.citation.fl_str_mv URIO, Elisandra Andreia. Caracterização citogenética clássica e molecular de trigos brasileiros. 2013. 123 f. Dissertação (Mestrado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, 2013.
dc.identifier.uri.fl_str_mv http://10.0.217.128:8080/jspui/handle/tede/554
identifier_str_mv URIO, Elisandra Andreia. Caracterização citogenética clássica e molecular de trigos brasileiros. 2013. 123 f. Dissertação (Mestrado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, 2013.
url http://10.0.217.128:8080/jspui/handle/tede/554
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 32148
dc.relation.confidence.fl_str_mv 600
600
600
dc.relation.department.fl_str_mv 545877
dc.relation.cnpq.fl_str_mv 9698989
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Agronomia
dc.publisher.initials.fl_str_mv UPF
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Faculdade de Agronomia e Medicina Veterinária – FAMV
publisher.none.fl_str_mv Universidade de Passo Fundo
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