Preservação das funções executivas em ratos expostos ao Status Epilepticus neonatal

• Autor(a) principal: Barbosa, Geraldo
• Data de Publicação: 2017
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações do Mackenzie
• Texto Completo: http://dspace.mackenzie.br/handle/10899/24554
• topic: convusões neonatais; pilocarpina; memória de trabalho; memória referência; aprendizagem associativa; flexibilidade comportamental; CNPQ::CIENCIAS DA SAUDE

Status Epilepticus (SE) is a transient occurrence of signs and / or symptoms due to excessive neuronal activity or abnormal synchrony in the brain. The transient term is used as a time path for the beginning and end of the crisis. The neonatal crisis is the most frequent manifestation of neurological dysfunction in the newborn, with a higher incidence in the first year of life, and presents risk of death, neuronal injury, neuronal death, neural network alterations or neurological and cognitive impairment of long depending on the type and duration of the crisis. However, about 40-50% of affected children develop normally. Studies in animal models demonstrate that neonatal seizures in rodents produce long-term neurological adverse effects such as: deficits in learning, visuospatial memory, impairment in cognitive exibility, autistic behavior, characterized by low preference for social novelty, discrimination deficit social and anxious type behavior. Based on the set of changes triggered by neonatal seizures in rodents, the model may be useful for inves- tigating the relationship between seizures in early development and executive functions. Thus, this project intends to evaluate behaviors related to the executive functions of animals that were exposed to neonatal SE. Male Wistar rats at the ninth day of life (P9) were submitted to SE by the administration of pilocarpine (350 mg / kg, ip) and controls received 0.9% saline (0.1 mL / 10 g). In P60 the animals were evaluated by the following paradigms: the operant conditioning box and the T. maze. The sample was composed of 25 animals, 11 controls and 14 experimental. These tests evaluate: visuospatial associative learning, memory consolidation, working memory, persevering behavior, inhibitory control and behavioral exibility. The data suggest that the experimental group in the operant conditioning box showed no impairment in visuospatial associative learning, that there was memory consolidation, did not present perseverant behavior, impairment in working memory and persevering behavior. In the T-maze, the experimental group maintained their working memory, reference memory and motivation, and it showed no impairment in behavioral exibility. The data suggest that a single neonatal SE episode does not impact associative learning, long-term memory, working and reference memories, and behavioral exibility.