Fitonematoides endoparasitas associados à cana-de-açúcar : interações e novo assinalamento
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7845 |
Resumo: | Meloidogyne incognita and Pratylenchus zeae are two of the most damaging nematodes of sugarcane, which are commonly present in Brazilian sugarcane fields causing severe losses. Another nematode widely known as damaging for the Brazilian agribusiness is the Meloidogyne enterolobii, which, recently, was reported as naturally occurrring in sugarcane field from Pernambuco and Rio Grande do Sul states. Aphelenchoides sp. despite being considered as a shoot parasitic nematode it is often associated with sugarcane rhizosphere, and some reports indicate its association with groundnut and some grasses which can damage these plants. This work aimed to evaluate the multiplication of M. incognita and M. enterolobii in both isolated and joint inoculation with P. zeae, to examine the effect of these interactions in initial sugarcane growing and to register the presence of Aphelenchoides spp. in sugarcane roots. Four experiments were performed, from which three were performed in parallel. In the first experiment, three different population densities of M. incognita and M. enterolobii were inoculated on both RB867515 and RB92579 sugarcane varieties. The second experiment followed the same experimental design at the first, but with previous infection of P. zeae (initial population=2000). The third experiment consisted of a prior inoculation de P. zeae followed by M. incognita and M. enterolobii inoculations in both sugarcane varieties. In the fourth experiment, roots were stained with acid fucsin and dissecated, then fixed, dehydrated, included in resin, sectioned e colored with toluidine blue. Meloidogyne enterolobii did not develop well in both sugarcane varieties even after 120 days of inoculation. Meloidogyne incognita reached high population levels when in single infections, but its population was reduced when in mixed infections with P. zeae. The mixed infections of P. zeae and M. incognita reduce both fresh and dried shoot weight. In mixed infectons P. zeae and M. incognita suppress each other. We also report Aphelenchoides sp. inside the roots in microscopy mount and histopathological sections under optical microscope, but this nematode did not induce any symptom of infection on plant. |
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GUIMARÃES, Lilian Margarete PaesPEDROSA, Elvira Maria RegisMARANHÃO, Sandra Roberta Vaz LiraGUIMARÃES, Lilian Margarete PaesPEDROSA, Elvira Maria RegisMARANHÃO, Sandra Roberta Vaz LiraLEITE, Raycenne Rosa2019-02-18T12:16:52Z2017-02-22LEITE, Raycenne Rosa. Fitonematoides endoparasitas associados à cana-de-açúcar : interações e novo assinalamento. 2017. 83 f. Dissertação (Programa de Pós-Graduação em Fitopatologia) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7845Meloidogyne incognita and Pratylenchus zeae are two of the most damaging nematodes of sugarcane, which are commonly present in Brazilian sugarcane fields causing severe losses. Another nematode widely known as damaging for the Brazilian agribusiness is the Meloidogyne enterolobii, which, recently, was reported as naturally occurrring in sugarcane field from Pernambuco and Rio Grande do Sul states. Aphelenchoides sp. despite being considered as a shoot parasitic nematode it is often associated with sugarcane rhizosphere, and some reports indicate its association with groundnut and some grasses which can damage these plants. This work aimed to evaluate the multiplication of M. incognita and M. enterolobii in both isolated and joint inoculation with P. zeae, to examine the effect of these interactions in initial sugarcane growing and to register the presence of Aphelenchoides spp. in sugarcane roots. Four experiments were performed, from which three were performed in parallel. In the first experiment, three different population densities of M. incognita and M. enterolobii were inoculated on both RB867515 and RB92579 sugarcane varieties. The second experiment followed the same experimental design at the first, but with previous infection of P. zeae (initial population=2000). The third experiment consisted of a prior inoculation de P. zeae followed by M. incognita and M. enterolobii inoculations in both sugarcane varieties. In the fourth experiment, roots were stained with acid fucsin and dissecated, then fixed, dehydrated, included in resin, sectioned e colored with toluidine blue. Meloidogyne enterolobii did not develop well in both sugarcane varieties even after 120 days of inoculation. Meloidogyne incognita reached high population levels when in single infections, but its population was reduced when in mixed infections with P. zeae. The mixed infections of P. zeae and M. incognita reduce both fresh and dried shoot weight. In mixed infectons P. zeae and M. incognita suppress each other. We also report Aphelenchoides sp. inside the roots in microscopy mount and histopathological sections under optical microscope, but this nematode did not induce any symptom of infection on plant.Meloidogyne incognita e Pratylenchus zeae estão entre os nematoides mais nocivos à cana-de-açúcar. Comumente são encontrados nos canaviais brasileiros causando sérios problemas. Outro nematoide que causa sérios danos a culturas do agronegócio nacional é Meloidogyne enterolobii e recentemente foi encontrado ocorrendo naturalmente em campos de cana-de-açúcar nos estados de Pernambuco e Rio Grande do Sul. Aphelenchoides sp., mesmo sendo um nematoide parasita de parte aérea frequentemente é encontrado associado a rizosfera de cana-de-açúcar. Havendo relatos de espécies de Aphelenchoides causando danos em raízes de amendoim e algumas gramíneas. Os objetivos deste trabalho foram avaliar a multiplicação de M. incognita e M. enterolobii em inoculação única e em inoculação conjunta com P. zeae, verificar o efeito dessa interação no desenvolvimento inicial da cana-de-açúcar e registrar a presença de Aphelenchoides spp. em raízes de cana-de-açúcar. Quatro experimentos foram montados, sendo três deles conduzidos paralelamente. No primeiro experimento, três densidades populacionais de M. incognita e M. enterolobii foram inoculados nas variedades RB867515 e RB92579 de cana-de-açúcar. O segundo experimento seguiu o mesmo delineamento do primeiro, tendo como diferença a infestação prévia com P. zeae (Pi= 2000). No terceiro experimento, foram feitas infestações com P. zeae seguidas com M. incognita e M. enterolobii nas variedades de cana-de-açúcar. No quarto experimento, as raízes foram coloridas com fucsina ácida e em seguida dissecadas, e também fixadas, desidratadas, incluídas em resina, microseccionadas e coloridas com azul de touidina. Meloidogyne enterolobii não teve bom desenvolvimento nas variedades de cana 120 dias após a infecção. Meloidogyne incognita alcançou altos níveis populacionais quando em infecções isoladas, contudo em infecções mistas com P. zeae teve uma redução populacional. Menor biomassa fresca e seca de cana-de-açúcar foi obtida na infestação conjunta de P. zeae e M. incognita. Em infecções mistas, P. zeae e M. incognita se suprimiam mutuamente. Foi possível observar Aphelenchoides sp. dentro das raízes em montagem microscópica e em cortes histopatológicos sob microscópio óptico. Nenhum sintoma da infecção deste nematoide foi encontrado na planta.Submitted by Mario BC (mario@bc.ufrpe.br) on 2019-02-18T12:16:52Z No. of bitstreams: 1 Raycenne Rosa Leite.pdf: 1146890 bytes, checksum: 7da0ff4813a79cda8b2f8f25e9acfa8c (MD5)Made available in DSpace on 2019-02-18T12:16:52Z (GMT). No. of bitstreams: 1 Raycenne Rosa Leite.pdf: 1146890 bytes, checksum: 7da0ff4813a79cda8b2f8f25e9acfa8c (MD5) Previous issue date: 2017-02-22Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em FitopatologiaUFRPEBrasilDepartamento de AgronomiaMeloidogynePratylenchusInfecção mistaCana-de-açúcarSaccharumFITOSSANIDADE::FITOPATOLOGIAFitonematoides endoparasitas associados à cana-de-açúcar : interações e novo assinalamentoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis1343367238723626701600600600600-6800553879972229205-6207026424523013504-2555911436985713659info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALRaycenne Rosa Leite.pdfRaycenne Rosa Leite.pdfapplication/pdf1146890http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7845/2/Raycenne+Rosa+Leite.pdf7da0ff4813a79cda8b2f8f25e9acfa8cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7845/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/78452019-02-18 09:16:52.779oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2019-02-18T12:16:52Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Fitonematoides endoparasitas associados à cana-de-açúcar : interações e novo assinalamento |
title |
Fitonematoides endoparasitas associados à cana-de-açúcar : interações e novo assinalamento |
spellingShingle |
Fitonematoides endoparasitas associados à cana-de-açúcar : interações e novo assinalamento LEITE, Raycenne Rosa Meloidogyne Pratylenchus Infecção mista Cana-de-açúcar Saccharum FITOSSANIDADE::FITOPATOLOGIA |
title_short |
Fitonematoides endoparasitas associados à cana-de-açúcar : interações e novo assinalamento |
title_full |
Fitonematoides endoparasitas associados à cana-de-açúcar : interações e novo assinalamento |
title_fullStr |
Fitonematoides endoparasitas associados à cana-de-açúcar : interações e novo assinalamento |
title_full_unstemmed |
Fitonematoides endoparasitas associados à cana-de-açúcar : interações e novo assinalamento |
title_sort |
Fitonematoides endoparasitas associados à cana-de-açúcar : interações e novo assinalamento |
author |
LEITE, Raycenne Rosa |
author_facet |
LEITE, Raycenne Rosa |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
GUIMARÃES, Lilian Margarete Paes |
dc.contributor.advisor-co1.fl_str_mv |
PEDROSA, Elvira Maria Regis |
dc.contributor.advisor-co2.fl_str_mv |
MARANHÃO, Sandra Roberta Vaz Lira |
dc.contributor.referee1.fl_str_mv |
GUIMARÃES, Lilian Margarete Paes |
dc.contributor.referee2.fl_str_mv |
PEDROSA, Elvira Maria Regis |
dc.contributor.referee3.fl_str_mv |
MARANHÃO, Sandra Roberta Vaz Lira |
dc.contributor.author.fl_str_mv |
LEITE, Raycenne Rosa |
contributor_str_mv |
GUIMARÃES, Lilian Margarete Paes PEDROSA, Elvira Maria Regis MARANHÃO, Sandra Roberta Vaz Lira GUIMARÃES, Lilian Margarete Paes PEDROSA, Elvira Maria Regis MARANHÃO, Sandra Roberta Vaz Lira |
dc.subject.por.fl_str_mv |
Meloidogyne Pratylenchus Infecção mista Cana-de-açúcar Saccharum |
topic |
Meloidogyne Pratylenchus Infecção mista Cana-de-açúcar Saccharum FITOSSANIDADE::FITOPATOLOGIA |
dc.subject.cnpq.fl_str_mv |
FITOSSANIDADE::FITOPATOLOGIA |
description |
Meloidogyne incognita and Pratylenchus zeae are two of the most damaging nematodes of sugarcane, which are commonly present in Brazilian sugarcane fields causing severe losses. Another nematode widely known as damaging for the Brazilian agribusiness is the Meloidogyne enterolobii, which, recently, was reported as naturally occurrring in sugarcane field from Pernambuco and Rio Grande do Sul states. Aphelenchoides sp. despite being considered as a shoot parasitic nematode it is often associated with sugarcane rhizosphere, and some reports indicate its association with groundnut and some grasses which can damage these plants. This work aimed to evaluate the multiplication of M. incognita and M. enterolobii in both isolated and joint inoculation with P. zeae, to examine the effect of these interactions in initial sugarcane growing and to register the presence of Aphelenchoides spp. in sugarcane roots. Four experiments were performed, from which three were performed in parallel. In the first experiment, three different population densities of M. incognita and M. enterolobii were inoculated on both RB867515 and RB92579 sugarcane varieties. The second experiment followed the same experimental design at the first, but with previous infection of P. zeae (initial population=2000). The third experiment consisted of a prior inoculation de P. zeae followed by M. incognita and M. enterolobii inoculations in both sugarcane varieties. In the fourth experiment, roots were stained with acid fucsin and dissecated, then fixed, dehydrated, included in resin, sectioned e colored with toluidine blue. Meloidogyne enterolobii did not develop well in both sugarcane varieties even after 120 days of inoculation. Meloidogyne incognita reached high population levels when in single infections, but its population was reduced when in mixed infections with P. zeae. The mixed infections of P. zeae and M. incognita reduce both fresh and dried shoot weight. In mixed infectons P. zeae and M. incognita suppress each other. We also report Aphelenchoides sp. inside the roots in microscopy mount and histopathological sections under optical microscope, but this nematode did not induce any symptom of infection on plant. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-02-22 |
dc.date.accessioned.fl_str_mv |
2019-02-18T12:16:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
LEITE, Raycenne Rosa. Fitonematoides endoparasitas associados à cana-de-açúcar : interações e novo assinalamento. 2017. 83 f. Dissertação (Programa de Pós-Graduação em Fitopatologia) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7845 |
identifier_str_mv |
LEITE, Raycenne Rosa. Fitonematoides endoparasitas associados à cana-de-açúcar : interações e novo assinalamento. 2017. 83 f. Dissertação (Programa de Pós-Graduação em Fitopatologia) - Universidade Federal Rural de Pernambuco, Recife. |
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http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7845 |
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Universidade Federal Rural de Pernambuco |
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