Produção de biossurfactante por Bacillus Cereus UCP 1615 com potencial para aplicações biotecnológicas industriais
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8772 |
Resumo: | The present study characterized and evaluated the biosurfactant produced by Bacillus cereus UCP 1615 in various industrial applications. The production of the biomolecule was carried out in a low-cost medium composed of 2% residual frying soybean oil and 0.12% peptone. The interfacial tension reducing capacity and the critical micelle concentration (CMC) were defined as 6 mN/m and 500 mg/L, respectively. Large-scale production was carried out in 1.2, 3.0 and 50 L bioreactors, reaching surface tension values of 28.7, 27.5 and 32 mN/m and yields around 4.3, 4.6 and 4.1 g/L, respectively. The biosurfactant was classified as an anionic lipopeptide from analyzes to determine ionic charge, thin layer chromatography (TLC), gas chromatography (GC) and nuclear magnetic resonance (NMR). Long-term conservation tests with the additive potassium sorbate (0.2%) demonstrated the stability of the surface-active properties of the biosurfactant, suggesting its application in a possible commercial formulation. To evaluate the application of the biosurfactant in environmental processes, it was applied in tests containing seawater, soil and rocks contaminated with petroleum derivatives and its toxicity was also evaluated against marine organisms Artemia salina (microcrustacean), Poecilia vivipara (fish) and Anomalocardia brasiliana (bivalve) and to the seeds of vegetables Brassica oleracea (cabbage), Solanum lycopersicum (tomato) and Cucumis anguria (gherkin). The biosurfactant proved to be efficient and biocompatible for bioremediation tests in seawater, promoting the growth of the microbiota, and showed removal rates of hydrophobic compounds in sandy soil and rocks of 84 and 69%, respectively. The application of the biomolecule as a bioemulsifier in food was also evaluated through its use in the composition of a cookie type cookie, with cytotoxicity using the L929 and Vero cell lines and the antioxidant activity determined. A biscuit with preserved typical characteristics and benefits compared to the standard without biosurfactant was obtained. Cytotoxicity tests reinforced the biosurfactant's biocompatibility, and the antioxidant activity indicative corroborated with the biosurfactant's contribution to the biscuit preservation. The biosurfactant was also tested as a stabilizer in the synthesis of silver nanoparticles as an antimicrobial agent. Nanoparticles of size around 20 nm were obtained using the green Tollens method. The nanoparticles showed fungicidal activity against human and plant pathogenic fungal genera. The results obtained demonstrate that the biosurfactant from Bacillus cereus UCP 1615 has potential for use in several economic sectors, proving the biotechnological versatility of this biomolecule and its industrial importance. |
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SARUBBO, Leonie AsforaRUFINO, Raquel DinizSANTOS, Valdemir Alexandre dosLUNA, Juliana Moura deSILVA, Rita de Cássia Freire Soares daGUERRA, Jenyffer Medeiros Camposhttp://lattes.cnpq.br/3893611639669082DURVAL, Italo José Batista2022-12-21T19:31:54Z2021-07-30DURVAL, Italo José Batista. Produção de biossurfactante por Bacillus Cereus UCP 1615 com potencial para aplicações biotecnológicas industriais. 2021. 150 f. Tese (Programa de Pós-Graduação em Biotecnologia (Renorbio)) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8772The present study characterized and evaluated the biosurfactant produced by Bacillus cereus UCP 1615 in various industrial applications. The production of the biomolecule was carried out in a low-cost medium composed of 2% residual frying soybean oil and 0.12% peptone. The interfacial tension reducing capacity and the critical micelle concentration (CMC) were defined as 6 mN/m and 500 mg/L, respectively. Large-scale production was carried out in 1.2, 3.0 and 50 L bioreactors, reaching surface tension values of 28.7, 27.5 and 32 mN/m and yields around 4.3, 4.6 and 4.1 g/L, respectively. The biosurfactant was classified as an anionic lipopeptide from analyzes to determine ionic charge, thin layer chromatography (TLC), gas chromatography (GC) and nuclear magnetic resonance (NMR). Long-term conservation tests with the additive potassium sorbate (0.2%) demonstrated the stability of the surface-active properties of the biosurfactant, suggesting its application in a possible commercial formulation. To evaluate the application of the biosurfactant in environmental processes, it was applied in tests containing seawater, soil and rocks contaminated with petroleum derivatives and its toxicity was also evaluated against marine organisms Artemia salina (microcrustacean), Poecilia vivipara (fish) and Anomalocardia brasiliana (bivalve) and to the seeds of vegetables Brassica oleracea (cabbage), Solanum lycopersicum (tomato) and Cucumis anguria (gherkin). The biosurfactant proved to be efficient and biocompatible for bioremediation tests in seawater, promoting the growth of the microbiota, and showed removal rates of hydrophobic compounds in sandy soil and rocks of 84 and 69%, respectively. The application of the biomolecule as a bioemulsifier in food was also evaluated through its use in the composition of a cookie type cookie, with cytotoxicity using the L929 and Vero cell lines and the antioxidant activity determined. A biscuit with preserved typical characteristics and benefits compared to the standard without biosurfactant was obtained. Cytotoxicity tests reinforced the biosurfactant's biocompatibility, and the antioxidant activity indicative corroborated with the biosurfactant's contribution to the biscuit preservation. The biosurfactant was also tested as a stabilizer in the synthesis of silver nanoparticles as an antimicrobial agent. Nanoparticles of size around 20 nm were obtained using the green Tollens method. The nanoparticles showed fungicidal activity against human and plant pathogenic fungal genera. The results obtained demonstrate that the biosurfactant from Bacillus cereus UCP 1615 has potential for use in several economic sectors, proving the biotechnological versatility of this biomolecule and its industrial importance.O presente estudo caracterizou e avaliou o biossurfactante produzido por Bacillus cereus UCP 1615 em diversas aplicações industriais. A produção da biomolécula foi feita em meio de baixo custo composto por 2% de óleo de soja residual de fritura e 0,12% de peptona. A capacidade de redução da tensão interfacial e a concentração micelar crítica (CMC) foram definidas como 6 mN/m e 500 mg/L, respectivamente. A produção em larga escala foi feita em biorreatores de 1,2, 3,0 e 50,0 L, alcançando valores de tensão superficial de 28,7, 27,5 e 32,0 mN/m e rendimentos em torno de 4,3, 4,6 e 4,1 g/L, respectivamente. O biossurfactante foi classificado como um lipopetídeo aniônico a partir de análises para determinação da carga iônica, cromatografia em camada delgada (TLC), cromatografia gasosa (GC) e ressonância magnética nuclear (RMN). Testes de conservação a longo prazo com o aditivo sorbato de potássio (0,2%) demonstraram a estabilidade das propriedades tensoativas do biossurfactante, sugerindo sua aplicação em uma possível formulação comercial. Para avaliar a aplicação do biossurfactante em processos ambientais, o mesmo foi aplicado em ensaios contendo água do mar, solo e rochas contaminadas com derivado de petróleo e sua toxicidade também foi avaliada frente ao microcrustáceo Artemia salina, ao peixe Poecilia vivipara e ao bivalve Anomalocardia brasiliana, bem como frente às sementes de vegetais Brassica oleracea (repolho), Solanum lycopersicum (tomate) e Cucumis anguria (maxixe). O biossurfactante mostrou-se eficiente e biocompatível para biorremediação em água do mar, promovendo o crescimento da microbiota, e ainda apresentou índices de remoção de compostos hidrofóbicos em solo arenoso e rochas de 84 e 69%, respectivamente. A aplicação da biomolécula como bioemulsificante em alimentos também foi avaliada através da sua utilização na composição de um biscoito tipo cookie, tendo a citotoxicidade utilizando as linhagens celulares L929 e Vero e a atividade antioxidante determinados. Um biscoito com características típicas preservadas e com benefícios em relação ao padrão sem biossurfactante foi obtido. Os testes de citotoxicidade reforçaram a biocompatibilidade do biossurfactante, e o indicativo de atividade antioxidante corroborou com a contribuição do biossurfactante para preservação do biscoito. O biossurfactante também foi avaliado como estabilizante na síntese de nanopartículas de prata como agente antimicrobiano. Nanopartículas de tamanho em torno de 20 nm foram obtidas através do método verde de Tollens. As nanopartículas apresentaram atividade fungicida diante de gêneros de fungos patogênicos humanos e vegetais. Os resultados obtidos demonstram que o biossurfactante de Bacillus cereus UCP 1615 tem potencial de utilização em diversos setores econômicos, comprovando a versatilidade biotecnológica desta biomolécula e sua importância industrial.Submitted by (lucia.rodrigues@ufrpe.br) on 2022-12-21T19:31:54Z No. of bitstreams: 1 Italo Jose Batista Durval.pdf: 1815528 bytes, checksum: cab851fb72bcd916e5a321992400269b (MD5)Made available in DSpace on 2022-12-21T19:31:54Z (GMT). No. of bitstreams: 1 Italo Jose Batista Durval.pdf: 1815528 bytes, checksum: cab851fb72bcd916e5a321992400269b (MD5) Previous issue date: 2021-07-30Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESConselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Biotecnologia (Renorbio)UFRPEBrasilRede Nordeste de BiotecnologiaBiorremediaçãoToxicidadeBiossurfactanteBacillus cereusBiotecnologiaPoluiçãoOUTROS::CIENCIASProdução de biossurfactante por Bacillus Cereus UCP 1615 com potencial para aplicações biotecnológicas industriaisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis7794227690756777355600600600600600-810457658845227642162099577914943238252075167498588264571-2555911436985713659info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALItalo Jose Batista Durval.pdfItalo Jose Batista Durval.pdfapplication/pdf1815528http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/8772/2/Italo+Jose+Batista+Durval.pdfcab851fb72bcd916e5a321992400269bMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/8772/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/87722022-12-21 16:31:54.597oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:37:24.718263Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Produção de biossurfactante por Bacillus Cereus UCP 1615 com potencial para aplicações biotecnológicas industriais |
title |
Produção de biossurfactante por Bacillus Cereus UCP 1615 com potencial para aplicações biotecnológicas industriais |
spellingShingle |
Produção de biossurfactante por Bacillus Cereus UCP 1615 com potencial para aplicações biotecnológicas industriais DURVAL, Italo José Batista Biorremediação Toxicidade Biossurfactante Bacillus cereus Biotecnologia Poluição OUTROS::CIENCIAS |
title_short |
Produção de biossurfactante por Bacillus Cereus UCP 1615 com potencial para aplicações biotecnológicas industriais |
title_full |
Produção de biossurfactante por Bacillus Cereus UCP 1615 com potencial para aplicações biotecnológicas industriais |
title_fullStr |
Produção de biossurfactante por Bacillus Cereus UCP 1615 com potencial para aplicações biotecnológicas industriais |
title_full_unstemmed |
Produção de biossurfactante por Bacillus Cereus UCP 1615 com potencial para aplicações biotecnológicas industriais |
title_sort |
Produção de biossurfactante por Bacillus Cereus UCP 1615 com potencial para aplicações biotecnológicas industriais |
author |
DURVAL, Italo José Batista |
author_facet |
DURVAL, Italo José Batista |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
SARUBBO, Leonie Asfora |
dc.contributor.advisor-co1.fl_str_mv |
RUFINO, Raquel Diniz |
dc.contributor.referee1.fl_str_mv |
SANTOS, Valdemir Alexandre dos |
dc.contributor.referee2.fl_str_mv |
LUNA, Juliana Moura de |
dc.contributor.referee3.fl_str_mv |
SILVA, Rita de Cássia Freire Soares da |
dc.contributor.referee4.fl_str_mv |
GUERRA, Jenyffer Medeiros Campos |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3893611639669082 |
dc.contributor.author.fl_str_mv |
DURVAL, Italo José Batista |
contributor_str_mv |
SARUBBO, Leonie Asfora RUFINO, Raquel Diniz SANTOS, Valdemir Alexandre dos LUNA, Juliana Moura de SILVA, Rita de Cássia Freire Soares da GUERRA, Jenyffer Medeiros Campos |
dc.subject.por.fl_str_mv |
Biorremediação Toxicidade Biossurfactante Bacillus cereus Biotecnologia Poluição |
topic |
Biorremediação Toxicidade Biossurfactante Bacillus cereus Biotecnologia Poluição OUTROS::CIENCIAS |
dc.subject.cnpq.fl_str_mv |
OUTROS::CIENCIAS |
description |
The present study characterized and evaluated the biosurfactant produced by Bacillus cereus UCP 1615 in various industrial applications. The production of the biomolecule was carried out in a low-cost medium composed of 2% residual frying soybean oil and 0.12% peptone. The interfacial tension reducing capacity and the critical micelle concentration (CMC) were defined as 6 mN/m and 500 mg/L, respectively. Large-scale production was carried out in 1.2, 3.0 and 50 L bioreactors, reaching surface tension values of 28.7, 27.5 and 32 mN/m and yields around 4.3, 4.6 and 4.1 g/L, respectively. The biosurfactant was classified as an anionic lipopeptide from analyzes to determine ionic charge, thin layer chromatography (TLC), gas chromatography (GC) and nuclear magnetic resonance (NMR). Long-term conservation tests with the additive potassium sorbate (0.2%) demonstrated the stability of the surface-active properties of the biosurfactant, suggesting its application in a possible commercial formulation. To evaluate the application of the biosurfactant in environmental processes, it was applied in tests containing seawater, soil and rocks contaminated with petroleum derivatives and its toxicity was also evaluated against marine organisms Artemia salina (microcrustacean), Poecilia vivipara (fish) and Anomalocardia brasiliana (bivalve) and to the seeds of vegetables Brassica oleracea (cabbage), Solanum lycopersicum (tomato) and Cucumis anguria (gherkin). The biosurfactant proved to be efficient and biocompatible for bioremediation tests in seawater, promoting the growth of the microbiota, and showed removal rates of hydrophobic compounds in sandy soil and rocks of 84 and 69%, respectively. The application of the biomolecule as a bioemulsifier in food was also evaluated through its use in the composition of a cookie type cookie, with cytotoxicity using the L929 and Vero cell lines and the antioxidant activity determined. A biscuit with preserved typical characteristics and benefits compared to the standard without biosurfactant was obtained. Cytotoxicity tests reinforced the biosurfactant's biocompatibility, and the antioxidant activity indicative corroborated with the biosurfactant's contribution to the biscuit preservation. The biosurfactant was also tested as a stabilizer in the synthesis of silver nanoparticles as an antimicrobial agent. Nanoparticles of size around 20 nm were obtained using the green Tollens method. The nanoparticles showed fungicidal activity against human and plant pathogenic fungal genera. The results obtained demonstrate that the biosurfactant from Bacillus cereus UCP 1615 has potential for use in several economic sectors, proving the biotechnological versatility of this biomolecule and its industrial importance. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021-07-30 |
dc.date.accessioned.fl_str_mv |
2022-12-21T19:31:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
DURVAL, Italo José Batista. Produção de biossurfactante por Bacillus Cereus UCP 1615 com potencial para aplicações biotecnológicas industriais. 2021. 150 f. Tese (Programa de Pós-Graduação em Biotecnologia (Renorbio)) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8772 |
identifier_str_mv |
DURVAL, Italo José Batista. Produção de biossurfactante por Bacillus Cereus UCP 1615 com potencial para aplicações biotecnológicas industriais. 2021. 150 f. Tese (Programa de Pós-Graduação em Biotecnologia (Renorbio)) - Universidade Federal Rural de Pernambuco, Recife. |
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openAccess |
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Universidade Federal Rural de Pernambuco |
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Programa de Pós-Graduação em Biotecnologia (Renorbio) |
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UFRPE |
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Rede Nordeste de Biotecnologia |
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Universidade Federal Rural de Pernambuco |
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