Detecção do Mycobacterium avium subespécie paratuberculosis em amostras de leite de bovinos na microrregião de Garanhuns, Pernambuco
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5062 |
Resumo: | The aim of the present study was to detect the IS900 region of MAP in bovine milk samples using the Real Time Polymerase Chain Reaction (qPCR) and conventional PCR, as well as to study the agreement between the tests used. In total, 121 bovine milk samples were collected from properties considered as a focus of MAP in the micro-region of Garanhuns, Pernambuco, Brazil. Of the 121 milk samples analyzed, it was possible to detect the DNA of MAP in 20 samples (16.52%) using conventional PCR and in 34 samples (28.09%) using qPCR. The DNA of the agent was detected on all of the six properties studied, ranging from 10.00% to 23.81% in the conventional PCR and from 10.00% to 36.84% in the qPCR. The agreement between qPCR and conventional PCR was moderate (Kappa =0.53; χ2 = 38.08; p<0.000). The sensitivity and specificity of conventional PCR in relation to qPCR was 50% and 96.6%, respectively. Based on these results, it was possible to conclude that the IS900 region of MAP is present in bovine milk in the micro-region of Garanhuns, Pernambuco. It was also possible to conclude that the qPCR technique used in the present study was more sensitive than conventional PCR in terms of detecting these bacteria in milk samples. |
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PINHEIRO JUNIOR, José WiltonMOTA, Rinaldo AparecidoYAMASAKI, Elise MiyukiCOSTA, Mateus Matiuzzi dahttp://lattes.cnpq.br/2607914573863928ALBUQUERQUE, Pedro Paulo Feitosa de2016-07-25T14:39:33Z2014-02-25ALBUQUERQUE, Pedro Paulo Feitosa de. Detecção do Mycobacterium avium subespécie paratuberculosis em amostras de leite de bovinos na microrregião de Garanhuns, Pernambuco. 2014.56 f. Dissertação (Programa de Pós-Graduação em Ciência Animal Tropical) - Universidade Federal Rural de Pernambuco,Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5062The aim of the present study was to detect the IS900 region of MAP in bovine milk samples using the Real Time Polymerase Chain Reaction (qPCR) and conventional PCR, as well as to study the agreement between the tests used. In total, 121 bovine milk samples were collected from properties considered as a focus of MAP in the micro-region of Garanhuns, Pernambuco, Brazil. Of the 121 milk samples analyzed, it was possible to detect the DNA of MAP in 20 samples (16.52%) using conventional PCR and in 34 samples (28.09%) using qPCR. The DNA of the agent was detected on all of the six properties studied, ranging from 10.00% to 23.81% in the conventional PCR and from 10.00% to 36.84% in the qPCR. The agreement between qPCR and conventional PCR was moderate (Kappa =0.53; χ2 = 38.08; p<0.000). The sensitivity and specificity of conventional PCR in relation to qPCR was 50% and 96.6%, respectively. Based on these results, it was possible to conclude that the IS900 region of MAP is present in bovine milk in the micro-region of Garanhuns, Pernambuco. It was also possible to conclude that the qPCR technique used in the present study was more sensitive than conventional PCR in terms of detecting these bacteria in milk samples.Objetivou-se com este estudo detectar a região IS900 do MAP em amostras de leite bovino utilizando-se as técnicas de Reação em Cadeia da Polimerase em Tempo Real (qPCR) e a PCR convencional, e realizar um estudo de concordância entre os testes utilizados. Foram coletadas 121 amostras de leite bovino de propriedades consideradas foco para o MAP pertencentes à microrregião de Garanhuns, Pernambuco, Brasil. Das 121 amostras de leite analisadas foi possível detectar o DNA do MAP em 20 (16,52%) amostras na PCR convencional e em 34 (28,09%) na qPCR. O DNA do agente foi detectado em todas as seis propriedades estudadas, variando de 10,00% a 23,81% na PCR convencional e de 10,00% a 36,84% na qPCR. A concordância entre a qPCR e PCR foi moderada (Kappa =0,53; χ2 = 38,08; p<0,000) e a sensibilidade e especificidade da PCR convencional com relação a qPCR foi de 50% e 96,6%, respectivamente. Conclui-se que o DNA da região IS900 do MAP está presente no leite bovino na região estudada e que a qPCR é uma técnica sensível e rápida para detecção desse agente em amostras de leite.Submitted by (edna.saturno@ufrpe.br) on 2016-07-25T14:39:33Z No. of bitstreams: 1 Pedro Paulo Feitosa de Albuquerque.pdf: 705656 bytes, checksum: 3b387f372f7375364f614a52bba0c4df (MD5)Made available in DSpace on 2016-07-25T14:39:33Z (GMT). No. of bitstreams: 1 Pedro Paulo Feitosa de Albuquerque.pdf: 705656 bytes, checksum: 3b387f372f7375364f614a52bba0c4df (MD5) Previous issue date: 2014-02-25Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Ciência Animal TropicalUFRPEBrasilDepartamento de Morfologia e Fisiologia AnimalAnálise de leiteSaúde públicaBovinoParatuberculoseMilk analysisPublic healthBovineParatuberculosisCIENCIAS AGRARIAS::MEDICINA VETERINARIADetecção do Mycobacterium avium subespécie paratuberculosis em amostras de leite de bovinos na microrregião de Garanhuns, Pernambucoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-6143187600765506511600600600600600-8922364187987396204453670264235017319-25559114369857136592075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5062/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALPedro Paulo Feitosa de Albuquerque.pdfPedro Paulo Feitosa de Albuquerque.pdfapplication/pdf705656http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/5062/2/Pedro+Paulo+Feitosa+de+Albuquerque.pdf3b387f372f7375364f614a52bba0c4dfMD52tede2/50622024-02-26 12:52:46.008oai:tede2:tede2/5062Tk9UQTogQ09MT1FVRSBBUVVJIEEgU1VBIFBSw5NQUklBIExJQ0VOw4dBCkVzdGEgbGljZW7Dp2EgZGUgZXhlbXBsbyDDqSBmb3JuZWNpZGEgYXBlbmFzIHBhcmEgZmlucyBpbmZvcm1hdGl2b3MuCgpMSUNFTsOHQSBERSBESVNUUklCVUnDh8ODTyBOw4NPLUVYQ0xVU0lWQQoKQ29tIGEgYXByZXNlbnRhw6fDo28gZGVzdGEgbGljZW7Dp2EsIHZvY8OqIChvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgClhYWCAoU2lnbGEgZGEgVW5pdmVyc2lkYWRlKSBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBwb2RlLCBzZW0gYWx0ZXJhciBvIGNvbnRlw7pkbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIApwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgU2lnbGEgZGUgVW5pdmVyc2lkYWRlIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPDs3BpYSBhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyAKbmVzdGEgbGljZW7Dp2EuIFZvY8OqIHRhbWLDqW0gZGVjbGFyYSBxdWUgbyBkZXDDs3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBuw6NvLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZcO6ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEHDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSAKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBTSUdMQSBERSAKVU5JVkVSU0lEQURFLCBWT0PDiiBERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVPDg08gQ09NTyAKVEFNQsOJTSBBUyBERU1BSVMgT0JSSUdBw4fDlUVTIEVYSUdJREFTIFBPUiBDT05UUkFUTyBPVSBBQ09SRE8uCgpBIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgo=Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:32:32.756668Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Detecção do Mycobacterium avium subespécie paratuberculosis em amostras de leite de bovinos na microrregião de Garanhuns, Pernambuco |
title |
Detecção do Mycobacterium avium subespécie paratuberculosis em amostras de leite de bovinos na microrregião de Garanhuns, Pernambuco |
spellingShingle |
Detecção do Mycobacterium avium subespécie paratuberculosis em amostras de leite de bovinos na microrregião de Garanhuns, Pernambuco ALBUQUERQUE, Pedro Paulo Feitosa de Análise de leite Saúde pública Bovino Paratuberculose Milk analysis Public health Bovine Paratuberculosis CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Detecção do Mycobacterium avium subespécie paratuberculosis em amostras de leite de bovinos na microrregião de Garanhuns, Pernambuco |
title_full |
Detecção do Mycobacterium avium subespécie paratuberculosis em amostras de leite de bovinos na microrregião de Garanhuns, Pernambuco |
title_fullStr |
Detecção do Mycobacterium avium subespécie paratuberculosis em amostras de leite de bovinos na microrregião de Garanhuns, Pernambuco |
title_full_unstemmed |
Detecção do Mycobacterium avium subespécie paratuberculosis em amostras de leite de bovinos na microrregião de Garanhuns, Pernambuco |
title_sort |
Detecção do Mycobacterium avium subespécie paratuberculosis em amostras de leite de bovinos na microrregião de Garanhuns, Pernambuco |
author |
ALBUQUERQUE, Pedro Paulo Feitosa de |
author_facet |
ALBUQUERQUE, Pedro Paulo Feitosa de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
PINHEIRO JUNIOR, José Wilton |
dc.contributor.advisor-co1.fl_str_mv |
MOTA, Rinaldo Aparecido |
dc.contributor.referee1.fl_str_mv |
YAMASAKI, Elise Miyuki |
dc.contributor.referee2.fl_str_mv |
COSTA, Mateus Matiuzzi da |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2607914573863928 |
dc.contributor.author.fl_str_mv |
ALBUQUERQUE, Pedro Paulo Feitosa de |
contributor_str_mv |
PINHEIRO JUNIOR, José Wilton MOTA, Rinaldo Aparecido YAMASAKI, Elise Miyuki COSTA, Mateus Matiuzzi da |
dc.subject.por.fl_str_mv |
Análise de leite Saúde pública Bovino Paratuberculose |
topic |
Análise de leite Saúde pública Bovino Paratuberculose Milk analysis Public health Bovine Paratuberculosis CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Milk analysis Public health Bovine Paratuberculosis |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The aim of the present study was to detect the IS900 region of MAP in bovine milk samples using the Real Time Polymerase Chain Reaction (qPCR) and conventional PCR, as well as to study the agreement between the tests used. In total, 121 bovine milk samples were collected from properties considered as a focus of MAP in the micro-region of Garanhuns, Pernambuco, Brazil. Of the 121 milk samples analyzed, it was possible to detect the DNA of MAP in 20 samples (16.52%) using conventional PCR and in 34 samples (28.09%) using qPCR. The DNA of the agent was detected on all of the six properties studied, ranging from 10.00% to 23.81% in the conventional PCR and from 10.00% to 36.84% in the qPCR. The agreement between qPCR and conventional PCR was moderate (Kappa =0.53; χ2 = 38.08; p<0.000). The sensitivity and specificity of conventional PCR in relation to qPCR was 50% and 96.6%, respectively. Based on these results, it was possible to conclude that the IS900 region of MAP is present in bovine milk in the micro-region of Garanhuns, Pernambuco. It was also possible to conclude that the qPCR technique used in the present study was more sensitive than conventional PCR in terms of detecting these bacteria in milk samples. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-02-25 |
dc.date.accessioned.fl_str_mv |
2016-07-25T14:39:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
ALBUQUERQUE, Pedro Paulo Feitosa de. Detecção do Mycobacterium avium subespécie paratuberculosis em amostras de leite de bovinos na microrregião de Garanhuns, Pernambuco. 2014.56 f. Dissertação (Programa de Pós-Graduação em Ciência Animal Tropical) - Universidade Federal Rural de Pernambuco,Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5062 |
identifier_str_mv |
ALBUQUERQUE, Pedro Paulo Feitosa de. Detecção do Mycobacterium avium subespécie paratuberculosis em amostras de leite de bovinos na microrregião de Garanhuns, Pernambuco. 2014.56 f. Dissertação (Programa de Pós-Graduação em Ciência Animal Tropical) - Universidade Federal Rural de Pernambuco,Recife. |
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Universidade Federal Rural de Pernambuco |
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Universidade Federal Rural de Pernambuco |
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