Análise morfométrica e imunohistoquímica das gônadas da prole de matrizes induzidas ao hipotireoidismo e tratadas com melatonina
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8147 |
Resumo: | Fetal development is vulnerable to complications of hypothyroidism in pregnancy, leading to a high rate of miscarriages, premature births, and weight changes. Melatonin is a hormone that is closely related to the maintenance of gestation, in addition to regulating thyroid physiology. Our objective was to investigate the effect of the action of melatonin at 10 mg / kg in rats induced hypothyroidism during gestation, as well as in the gonads of their offspring. Fifteen pregnant rats were used, divided into 3 groups: GC: rats without induction to hypothyroidism; GH: rats induced hypothyroidism; GHM: rats induced hypothyroidism and treated with melatonin. Hypothyroidism was induced by oral administration of propylthiouracil (1mg / animal) and melatonin was administered subcutaneously (10mg / kg). Both treatments were performed during gestation and lactation. The gonads of the pups were collected for morphometric analysis and immunohistochemistry of cell proliferation and the thyroids of pups and matrices for morphometric analysis. The results showed that TSH dosing confirmed hypothyroidism in the matrices of the untreated group, where there was a significant reduction (p <0.05) when compared to the control and treated groups with melatonin. In the pups, there was no significant difference (p> 0.05) in TSH levels between groups. The weight of the ovaries, thyroids and the number of pups of the matrices with hypothyroidism presented a significant reduction (p <0.05) when compared to the other groups. Similarly in the thyroid, there was a decrease (p <0.05) in follicular diameter, height and epithelial proportion in the hypothyroid group. Body weight gain, however, showed a significant reduction (p <0.05) in the hypothyroid group when compared to the control group, but the melatonin group did not differ from any of the groups. The offspring of the matrices of the hypothyroid group showed a significant (p <0.05) reduction in body weight, gonads and thyroid weight, as well as the gonad cell proliferation index, when compared to controls and hypothalamus groups treated with melatonin. In view of the results, it can be concluded that the treatment with melatonin proved to be beneficial to prevent the effects of maternal hypothyroidism on the offspring of rats, reinforcing their ability to regulate organogenesis during gestation. |
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TEIXEIRA, Álvaro Aguiar CoelhoTEIXEIRA, Valéria WanderleyTEIXEIRA, Valéria WanderleySOUZA, Francisco de Assis LeiteSILVA, Welma Emídio dahttp://lattes.cnpq.br/0166078877642246ALBUQUERQUE, Yuri Mateus Lima de2019-07-12T15:11:07Z2019-01-18ALBUQUERQUE, Yuri Mateus Lima de. Análise morfométrica e imunohistoquímica das gônadas da prole de matrizes induzidas ao hipotireoidismo e tratadas com melatonina. 2019. 70 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8147Fetal development is vulnerable to complications of hypothyroidism in pregnancy, leading to a high rate of miscarriages, premature births, and weight changes. Melatonin is a hormone that is closely related to the maintenance of gestation, in addition to regulating thyroid physiology. Our objective was to investigate the effect of the action of melatonin at 10 mg / kg in rats induced hypothyroidism during gestation, as well as in the gonads of their offspring. Fifteen pregnant rats were used, divided into 3 groups: GC: rats without induction to hypothyroidism; GH: rats induced hypothyroidism; GHM: rats induced hypothyroidism and treated with melatonin. Hypothyroidism was induced by oral administration of propylthiouracil (1mg / animal) and melatonin was administered subcutaneously (10mg / kg). Both treatments were performed during gestation and lactation. The gonads of the pups were collected for morphometric analysis and immunohistochemistry of cell proliferation and the thyroids of pups and matrices for morphometric analysis. The results showed that TSH dosing confirmed hypothyroidism in the matrices of the untreated group, where there was a significant reduction (p <0.05) when compared to the control and treated groups with melatonin. In the pups, there was no significant difference (p> 0.05) in TSH levels between groups. The weight of the ovaries, thyroids and the number of pups of the matrices with hypothyroidism presented a significant reduction (p <0.05) when compared to the other groups. Similarly in the thyroid, there was a decrease (p <0.05) in follicular diameter, height and epithelial proportion in the hypothyroid group. Body weight gain, however, showed a significant reduction (p <0.05) in the hypothyroid group when compared to the control group, but the melatonin group did not differ from any of the groups. The offspring of the matrices of the hypothyroid group showed a significant (p <0.05) reduction in body weight, gonads and thyroid weight, as well as the gonad cell proliferation index, when compared to controls and hypothalamus groups treated with melatonin. In view of the results, it can be concluded that the treatment with melatonin proved to be beneficial to prevent the effects of maternal hypothyroidism on the offspring of rats, reinforcing their ability to regulate organogenesis during gestation.O desenvolvimento fetal é vulnerável a complicações originadas pelo hipotireoidismo na gestação, levando à uma taxa elevada de abortos, partos prematuros e alteração no peso. A melatonina é um hormônio que está intimamente relacionado à manutenção da gestação, além de regular a fisiologia tireoidiana. Nosso objetivo foi investigar o efeito da ação da melatonina, na dose 10mg/kg, em ratas induzidas ao hipotireoidismo durante a gestação, bem como nas gônadas da sua prole. Foram utilizadas 15 ratas prenhes, divididas em 3 grupos: GC: ratas sem indução ao hipotireoidismo; GH: ratas induzidas ao hipotireoidismo; GHM: ratas induzidas ao hipotireoidismo e tratadas com melatonina. O hipotireoidismo foi induzido pela administração oral de propiltiouracil (1mg/animal) e a melatonina foi administrada via subcutânea (10mg/kg). Ambos os tratamentos foram realizados durante a gestação e lactação. Foram coletadas as gônadas dos filhotes para análise morfométrica e imunohistoquímica de proliferação celular e as tireóides dos filhotes e matrizes para análise morfométrica. Os resultados mostraram que a dosagem de TSH confirmou o hipotireoidismo nas matrizes do grupo sem tratamento, onde houve uma redução significativa (p<0,05) quando comparados com os grupos controle e tratado com melatonina. Já nos filhotes, não houve diferença significativa (p>0,05) nos níveis de TSH entre os grupos. O peso dos ovários, tireóides e o número de filhotes das matrizes com hipotireoidismo apresentaram redução signiticativa (p<0,05) quando comparados aos demais grupos. Da mesma forma na tireóide houve diminuição (p<0,05) do diâmetro folicular, da altura e proporção epitelial no grupo hipotireoidético. Já o ganho de peso corporal mostrou uma redução significativa (p<0,05) do grupo hipotireoidético quando comparado ao grupo controle, porém o grupo tratado com melatonina não diferiu de nenhum dos grupos. A prole das matrizes do grupo hipotireoidismo apresentou redução significativa (p<0,05) do peso corporal, peso das gônadas e da tireóide, além do índice de proliferação celular das gônadas, quando comparados aos filhotes dos grupos controle e hipotireoidismo tratado com melatonina. Diante dos resultados, pode-se concluir que o tratamento com melatonina se mostrou benéfico para prevenir os efeitos do hipotireoidismo materno na prole de ratos, reforçando sua capacidade de regular a organogênese durante a gestação.Submitted by Mario BC (mario@bc.ufrpe.br) on 2019-07-12T15:11:07Z No. of bitstreams: 1 Yuri Mateus Lima de Albuquerque.pdf: 3317780 bytes, checksum: e7b715b1405bc25764cc47521eebb8f2 (MD5)Made available in DSpace on 2019-07-12T15:11:07Z (GMT). No. of bitstreams: 1 Yuri Mateus Lima de Albuquerque.pdf: 3317780 bytes, checksum: e7b715b1405bc25764cc47521eebb8f2 (MD5) Previous issue date: 2019-01-18application/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Biociência AnimalUFRPEBrasilDepartamento de Morfologia e Fisiologia AnimalMorfometriaImunohistoquímicaMelatoninaTireóideGestaçãoCIENCIAS AGRARIAS::MEDICINA VETERINARIAAnálise morfométrica e imunohistoquímica das gônadas da prole de matrizes induzidas ao hipotireoidismo e tratadas com melatoninainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1510757014399315592600600600-8922364187987396204453670264235017319info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALYuri Mateus Lima de Albuquerque.pdfYuri Mateus Lima de Albuquerque.pdfapplication/pdf3317780http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/8147/2/Yuri+Mateus+Lima+de+Albuquerque.pdfe7b715b1405bc25764cc47521eebb8f2MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/8147/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/81472019-07-12 12:11:07.256oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:36:35.606536Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Análise morfométrica e imunohistoquímica das gônadas da prole de matrizes induzidas ao hipotireoidismo e tratadas com melatonina |
title |
Análise morfométrica e imunohistoquímica das gônadas da prole de matrizes induzidas ao hipotireoidismo e tratadas com melatonina |
spellingShingle |
Análise morfométrica e imunohistoquímica das gônadas da prole de matrizes induzidas ao hipotireoidismo e tratadas com melatonina ALBUQUERQUE, Yuri Mateus Lima de Morfometria Imunohistoquímica Melatonina Tireóide Gestação CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Análise morfométrica e imunohistoquímica das gônadas da prole de matrizes induzidas ao hipotireoidismo e tratadas com melatonina |
title_full |
Análise morfométrica e imunohistoquímica das gônadas da prole de matrizes induzidas ao hipotireoidismo e tratadas com melatonina |
title_fullStr |
Análise morfométrica e imunohistoquímica das gônadas da prole de matrizes induzidas ao hipotireoidismo e tratadas com melatonina |
title_full_unstemmed |
Análise morfométrica e imunohistoquímica das gônadas da prole de matrizes induzidas ao hipotireoidismo e tratadas com melatonina |
title_sort |
Análise morfométrica e imunohistoquímica das gônadas da prole de matrizes induzidas ao hipotireoidismo e tratadas com melatonina |
author |
ALBUQUERQUE, Yuri Mateus Lima de |
author_facet |
ALBUQUERQUE, Yuri Mateus Lima de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
TEIXEIRA, Álvaro Aguiar Coelho |
dc.contributor.advisor-co1.fl_str_mv |
TEIXEIRA, Valéria Wanderley |
dc.contributor.referee1.fl_str_mv |
TEIXEIRA, Valéria Wanderley |
dc.contributor.referee2.fl_str_mv |
SOUZA, Francisco de Assis Leite |
dc.contributor.referee3.fl_str_mv |
SILVA, Welma Emídio da |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0166078877642246 |
dc.contributor.author.fl_str_mv |
ALBUQUERQUE, Yuri Mateus Lima de |
contributor_str_mv |
TEIXEIRA, Álvaro Aguiar Coelho TEIXEIRA, Valéria Wanderley TEIXEIRA, Valéria Wanderley SOUZA, Francisco de Assis Leite SILVA, Welma Emídio da |
dc.subject.por.fl_str_mv |
Morfometria Imunohistoquímica Melatonina Tireóide Gestação |
topic |
Morfometria Imunohistoquímica Melatonina Tireóide Gestação CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Fetal development is vulnerable to complications of hypothyroidism in pregnancy, leading to a high rate of miscarriages, premature births, and weight changes. Melatonin is a hormone that is closely related to the maintenance of gestation, in addition to regulating thyroid physiology. Our objective was to investigate the effect of the action of melatonin at 10 mg / kg in rats induced hypothyroidism during gestation, as well as in the gonads of their offspring. Fifteen pregnant rats were used, divided into 3 groups: GC: rats without induction to hypothyroidism; GH: rats induced hypothyroidism; GHM: rats induced hypothyroidism and treated with melatonin. Hypothyroidism was induced by oral administration of propylthiouracil (1mg / animal) and melatonin was administered subcutaneously (10mg / kg). Both treatments were performed during gestation and lactation. The gonads of the pups were collected for morphometric analysis and immunohistochemistry of cell proliferation and the thyroids of pups and matrices for morphometric analysis. The results showed that TSH dosing confirmed hypothyroidism in the matrices of the untreated group, where there was a significant reduction (p <0.05) when compared to the control and treated groups with melatonin. In the pups, there was no significant difference (p> 0.05) in TSH levels between groups. The weight of the ovaries, thyroids and the number of pups of the matrices with hypothyroidism presented a significant reduction (p <0.05) when compared to the other groups. Similarly in the thyroid, there was a decrease (p <0.05) in follicular diameter, height and epithelial proportion in the hypothyroid group. Body weight gain, however, showed a significant reduction (p <0.05) in the hypothyroid group when compared to the control group, but the melatonin group did not differ from any of the groups. The offspring of the matrices of the hypothyroid group showed a significant (p <0.05) reduction in body weight, gonads and thyroid weight, as well as the gonad cell proliferation index, when compared to controls and hypothalamus groups treated with melatonin. In view of the results, it can be concluded that the treatment with melatonin proved to be beneficial to prevent the effects of maternal hypothyroidism on the offspring of rats, reinforcing their ability to regulate organogenesis during gestation. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-07-12T15:11:07Z |
dc.date.issued.fl_str_mv |
2019-01-18 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
ALBUQUERQUE, Yuri Mateus Lima de. Análise morfométrica e imunohistoquímica das gônadas da prole de matrizes induzidas ao hipotireoidismo e tratadas com melatonina. 2019. 70 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8147 |
identifier_str_mv |
ALBUQUERQUE, Yuri Mateus Lima de. Análise morfométrica e imunohistoquímica das gônadas da prole de matrizes induzidas ao hipotireoidismo e tratadas com melatonina. 2019. 70 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife. |
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http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8147 |
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por |
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openAccess |
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Universidade Federal Rural de Pernambuco |
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UFRPE |
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Universidade Federal Rural de Pernambuco |
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