Caracterização de fontes de resistência genética contra espécies de Berkeleyomyces e Septoria lactucae em Lactuca sativa

Detalhes bibliográficos
Autor(a) principal: PERDOMO, David Nataren
Data de Publicação: 2022
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9412
Resumo: Lettuce (Lactuca sativa L.) is the most important leafy vegetable in Brazil and across the world. Lettuce cultivars are grouped into different morphotypes according to their morphological characteristics with special emphasis on the segments ‘Butterhead’, ‘Crispy loose-leaf’, ‘Iceberg’, ‘Cos/Romaine’, ‘Batavian’, and ‘Mimosa’. The yield of lettuce cultivars from the morphotypes ‘Iceberg’ and ‘Butterhead’ have been severely affected by two fungal diseases: Septoria leaf spot (caused by isolates of Septoria lactucae) and black root rot disease (caused by isolates of Berkeleyomyces basicola and B. rouxiae). The present investigation aimed to identify and characterize sources of resistance to S. lactucae as well as against the two Berkeleyomyces species in L. sativa germplasm. Genetic inheritance studies of resistance against isolates of the two Berkeleyomyces species were also performed. Three sets of experiments were carried out at Embrapa Hortaliças in Brasilia-DF during 2021 and 2022. In Chapter 2, forty-two (42) lettuce accessions were evaluated under field conditions (during the rainy season) for reaction to S. lactucae. Nine cultivars categorized as resistant under field conditions were subsequently inoculated with four isolates of S. lactucae under greenhouse conditions. The Area Under the Disease Progress Curve (AACPD) values were significantly lower for the cultivars ‘BRS Mediterrânea’ and ‘Vanda’ (from the ‘Crispy loose-leaf’ morphotype). The other cultivars displayed a tolerance reaction, with intermediate AACPD values, except for the cultivar ‘Rubi’ (control), which displayed moderate susceptibility to the pathogen. In Chapter 3, 68 lettuce cultivars were initially inoculated with an isolate of B. basicola. A second experiment was conducted using 33 cultivars classified as resistant in the first experiment. These accessions were subsequently inoculated with four isolates (two of B. basicola and two of B. rouxiae). A strong correlation was observed between resistance/susceptibility to B. basicola and B. rouxiae. The morphotypes ‘Cos/Romaine’ and ‘Batavian’ showed a higher frequency of resistant accessions. Variation in the reaction to these pathogens was observed among accessions of the morphotypes ‘Crispy loose-leaf’, ‘Mimosa’ and ‘Iceberg’. Most of the lettuces of the ‘Butterhead’ morphotype displayed a susceptible reaction. In Chapter 4, genetic inheritance studies for resistance to both Berkeleyomyces species were carried out under controlled conditions. Different crosses were carried out between the resistant parent ‘La Brillante’ (from the ‘Batavian’ morphotype) and the cultivar ‘Elisa’ (susceptible parent from the ‘Butterhead’ morphotype). Morphological analysis and molecular assays with RAPD markers were used to confirm the hybrid origin of lettuce plants obtained via controlled crosses. Two individual plants were confirmed as F1 hybrids via genotyping and then self-fertilized to obtain two segregating F2 populations. A total of 413 F2 plants were inoculated separately with B. rouxiae (247 plants) and B. basicola (166 plants). Chi-square (χ2) tests at 5% were performed to estimate the best fit of the observed segregation patterns to the predicted Mendelian ratios. Highly contrasting responses were observed between the parents ‘La Brillante’ (resistant) and ‘Elisa’ (susceptible) after inoculation with the two fungi. Segregations in the F2 population displayed a good fit to a dominant monogenic model for resistance against both fungal species. In conclusion, the results obtained in the present work indicate that there are different resistance/susceptibility levels across the lettuce morphotypes in relation to Berkeleyomyces species and S. lactucae. The identification and genetic characterization of novel sources of resistance against these pathogens represents an important contribution to lettuce breeding. The management of these diseases using resistant cultivars can assure higher levels of sustainability in the cultivation of this vegetable in tropical and subtropical regions of Brazil and across the world.
id URPE_225c127bb2734f5cf4a9ba3feaa9f0eb
oai_identifier_str oai:tede2:tede2/9412
network_acronym_str URPE
network_name_str Biblioteca Digital de Teses e Dissertações da UFRPE
repository_id_str
spelling BOITEUX, Leonardo SilvaREIS, AiltonCABRAL, Cléia SantosCÂMARA, Marcos Paz SaraivaGOMES, André Angelo MedeirosNASCIMENTO, Warley MarcosGONZÁLEZ ARCOS, Matiashttp://lattes.cnpq.br/9708022704070422PERDOMO, David Nataren2023-10-18T20:51:00Z2022-11-18PERDOMO, David Nataren. Caracterização de fontes de resistência genética contra espécies de Berkeleyomyces e Septoria lactucae em Lactuca sativa. 2022. 102 f. Tese (Programa de Pós-Graduação em Fitopatologia) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9412Lettuce (Lactuca sativa L.) is the most important leafy vegetable in Brazil and across the world. Lettuce cultivars are grouped into different morphotypes according to their morphological characteristics with special emphasis on the segments ‘Butterhead’, ‘Crispy loose-leaf’, ‘Iceberg’, ‘Cos/Romaine’, ‘Batavian’, and ‘Mimosa’. The yield of lettuce cultivars from the morphotypes ‘Iceberg’ and ‘Butterhead’ have been severely affected by two fungal diseases: Septoria leaf spot (caused by isolates of Septoria lactucae) and black root rot disease (caused by isolates of Berkeleyomyces basicola and B. rouxiae). The present investigation aimed to identify and characterize sources of resistance to S. lactucae as well as against the two Berkeleyomyces species in L. sativa germplasm. Genetic inheritance studies of resistance against isolates of the two Berkeleyomyces species were also performed. Three sets of experiments were carried out at Embrapa Hortaliças in Brasilia-DF during 2021 and 2022. In Chapter 2, forty-two (42) lettuce accessions were evaluated under field conditions (during the rainy season) for reaction to S. lactucae. Nine cultivars categorized as resistant under field conditions were subsequently inoculated with four isolates of S. lactucae under greenhouse conditions. The Area Under the Disease Progress Curve (AACPD) values were significantly lower for the cultivars ‘BRS Mediterrânea’ and ‘Vanda’ (from the ‘Crispy loose-leaf’ morphotype). The other cultivars displayed a tolerance reaction, with intermediate AACPD values, except for the cultivar ‘Rubi’ (control), which displayed moderate susceptibility to the pathogen. In Chapter 3, 68 lettuce cultivars were initially inoculated with an isolate of B. basicola. A second experiment was conducted using 33 cultivars classified as resistant in the first experiment. These accessions were subsequently inoculated with four isolates (two of B. basicola and two of B. rouxiae). A strong correlation was observed between resistance/susceptibility to B. basicola and B. rouxiae. The morphotypes ‘Cos/Romaine’ and ‘Batavian’ showed a higher frequency of resistant accessions. Variation in the reaction to these pathogens was observed among accessions of the morphotypes ‘Crispy loose-leaf’, ‘Mimosa’ and ‘Iceberg’. Most of the lettuces of the ‘Butterhead’ morphotype displayed a susceptible reaction. In Chapter 4, genetic inheritance studies for resistance to both Berkeleyomyces species were carried out under controlled conditions. Different crosses were carried out between the resistant parent ‘La Brillante’ (from the ‘Batavian’ morphotype) and the cultivar ‘Elisa’ (susceptible parent from the ‘Butterhead’ morphotype). Morphological analysis and molecular assays with RAPD markers were used to confirm the hybrid origin of lettuce plants obtained via controlled crosses. Two individual plants were confirmed as F1 hybrids via genotyping and then self-fertilized to obtain two segregating F2 populations. A total of 413 F2 plants were inoculated separately with B. rouxiae (247 plants) and B. basicola (166 plants). Chi-square (χ2) tests at 5% were performed to estimate the best fit of the observed segregation patterns to the predicted Mendelian ratios. Highly contrasting responses were observed between the parents ‘La Brillante’ (resistant) and ‘Elisa’ (susceptible) after inoculation with the two fungi. Segregations in the F2 population displayed a good fit to a dominant monogenic model for resistance against both fungal species. In conclusion, the results obtained in the present work indicate that there are different resistance/susceptibility levels across the lettuce morphotypes in relation to Berkeleyomyces species and S. lactucae. The identification and genetic characterization of novel sources of resistance against these pathogens represents an important contribution to lettuce breeding. The management of these diseases using resistant cultivars can assure higher levels of sustainability in the cultivation of this vegetable in tropical and subtropical regions of Brazil and across the world.A alface (Lactuca sativa L.) é a hortaliça folhosa mais importante no Brasil e no mundo. As cultivares de alface são agrupadas em diferentes morfotipos com especial destaque para os segmentos ‘Lisa’, ‘Crespa’, ‘Americana’, ‘Romana’, ‘Batávia’ e ‘Mimosa’. Os níveis de produtividade desta hortaliça e, em especial, das cultivares dos morfotipos ‘Americana’ e ‘Lisa’ vêm sendo afetados por duas doenças de etiologia fúngica: a septoriose causada por isolados de Septoria lactucae e murchadeira (causada por isolados de Berkeleyomyces basicola e B. rouxiae). A presente investigação objetivou identificar e caracterizar fontes de resistência contra S. lactucae e contra as duas espécies de Berkeleyomyces em germoplasma de L. sativa. Foram também realizados estudos de herança genética da resistência contra isolados das duas espécies de Berkeleyomyces. Foram desenvolvidos grupos de experimentos no Centro Nacional de Pesquisa de Hortaliças (Embrapa Hortaliças), em Brasília-DF durante os anos 2021 e 2022. No Capítulo 2, foram avaliadas 42 cultivares de alface em condições de campo (durante a estação chuvosa) para reação ao fungo S. lactucae. Nove cultivares categorizadas como resistentes em condições de campo foram posteriormente inoculadas com quatro isolados de S. lactucae em condições de casa de vegetação. Os valores de Área Abaixo da Curva de Progresso da Doença (AACPD) foram significativamente menores para as cultivares ‘BRS Mediterrânea’ e ‘Vanda’ (do grupo varietal ‘Crespa’). As demais cultivares mostraram reação de tolerância, com valores intermediários de AACPD, com a exceção da cultivar ‘Rubi’ (testemunha) que mostrou moderada suscetibilidade ao patógeno. No Capítulo 3 foram avaliadas 68 cultivares de alface que foram inicialmente inoculadas com um isolado de B. basicola. Um segundo ensaio foi conduzido utilizando 33 cultivares classificadas como resistentes no primeiro ensaio. As plantas foram analisadas e inoculadas com quatro isolados (dois de B. basicola e dois de B. rouxiae). Foi observada uma forte correlação entre resistência/suscetibilidade para B. basicola e B. rouxiae. Os grupos varietais do tipo ‘Romana’ e ‘Batávia’ apresentaram uma frequência mais elevada de acessos resistentes. Houve variação intervarietal entre acessos dos morfotipos ‘Crespa’, ‘Mimosa’ e ‘Americana’. A maioria das alfaces do segmento ‘Lisa’ se mostrou suscetível. No Capítulo 4, foram realizados, em condições controladas, estudos de herança da resistência genética contra as duas espécies de Berkeleyomyces em alface. Foram realizados distintos cruzamentos entre a cultivar ‘La Brillante’ (parental resistente do segmento ‘Batávia’) com ‘Elisa’ (parental suscetível do segmento ‘Lisa’). Marcadores moleculares do tipo RAPD e análise morfológica foram utilizados para confirmar a origem híbrida de plantas obtidas via cruzamentos controlados. Duas plantas individuais foram confirmadas via genotipagem como sendo híbridas F1 e então autofecundadas para obtenção de duas populações F2 segregantes. Ao todo, 413 plantas F2 foram inoculadas separadamente com B. rouxiae (247 plantas) e B. basicola (166 plantas). Testes de qui-quadrado (χ2) a 5% foram conduzidos para estimar a adequação dos padrões de segregação observados às proporções Mendelianas previstas. Respostas altamente contrastantes foram confirmadas entre os parentais ‘La Brillante’ (resistente) e ‘Elisa’ (suscetível) após a inoculação com os dois fungos. Segregações na população F2 se ajustaram melhor a um modelo monogênico dominante para a resistência contra as duas espécies fúngicas. Em conclusão, os resultados obtidos no presente trabalho indicam haver distintos níveis de resistência/suscetibilidade em relação aos patógenos Berkeleyomyces e S. lactucae nos diferentes morfotipos de alface. A caracterização genética de novas fontes de resistência contra esses patógenos representa uma importante contribuição para melhoramento da alface. O manejo destas doenças via utilização de cultivares resistentes pode garantir níveis maiores de sustentabilidade do cultivo desta hortaliça em regiões tropicais e subtropicais do Brasil e do mundo.Submitted by (ana.araujo@ufrpe.br) on 2023-10-18T20:51:00Z No. of bitstreams: 1 David Nataren Perdomo.pdf: 1303095 bytes, checksum: de7a87a79f10738e1db0c24d4e4f3316 (MD5)Made available in DSpace on 2023-10-18T20:51:00Z (GMT). No. of bitstreams: 1 David Nataren Perdomo.pdf: 1303095 bytes, checksum: de7a87a79f10738e1db0c24d4e4f3316 (MD5) Previous issue date: 2022-11-18Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em FitopatologiaUFRPEBrasilDepartamento de AgronomiaAlfaceLactuca sativaResistência genéticaFungos fitopatogênicosSeptoria lactucaeBerkeleyomycesMelhoramento genético vegetalFITOSSANIDADE::FITOPATOLOGIACaracterização de fontes de resistência genética contra espécies de Berkeleyomyces e Septoria lactucae em Lactuca sativainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis1343367238723626701600600600600-6800553879972229205-62070264245230135042075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALDavid Nataren Perdomo.pdfDavid Nataren Perdomo.pdfapplication/pdf1303095http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/9412/2/David+Nataren+Perdomo.pdfde7a87a79f10738e1db0c24d4e4f3316MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/9412/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/94122023-10-18 17:51:00.828oai:tede2:tede2/9412Tk9UQTogQ09MT1FVRSBBUVVJIEEgU1VBIFBSw5NQUklBIExJQ0VOw4dBCkVzdGEgbGljZW7Dp2EgZGUgZXhlbXBsbyDDqSBmb3JuZWNpZGEgYXBlbmFzIHBhcmEgZmlucyBpbmZvcm1hdGl2b3MuCgpMSUNFTsOHQSBERSBESVNUUklCVUnDh8ODTyBOw4NPLUVYQ0xVU0lWQQoKQ29tIGEgYXByZXNlbnRhw6fDo28gZGVzdGEgbGljZW7Dp2EsIHZvY8OqIChvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgClhYWCAoU2lnbGEgZGEgVW5pdmVyc2lkYWRlKSBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBwb2RlLCBzZW0gYWx0ZXJhciBvIGNvbnRlw7pkbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIApwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgU2lnbGEgZGUgVW5pdmVyc2lkYWRlIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPDs3BpYSBhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyAKbmVzdGEgbGljZW7Dp2EuIFZvY8OqIHRhbWLDqW0gZGVjbGFyYSBxdWUgbyBkZXDDs3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBuw6NvLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZcO6ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEHDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSAKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBTSUdMQSBERSAKVU5JVkVSU0lEQURFLCBWT0PDiiBERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVPDg08gQ09NTyAKVEFNQsOJTSBBUyBERU1BSVMgT0JSSUdBw4fDlUVTIEVYSUdJREFTIFBPUiBDT05UUkFUTyBPVSBBQ09SRE8uCgpBIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgo=Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:38:14.493371Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false
dc.title.por.fl_str_mv Caracterização de fontes de resistência genética contra espécies de Berkeleyomyces e Septoria lactucae em Lactuca sativa
title Caracterização de fontes de resistência genética contra espécies de Berkeleyomyces e Septoria lactucae em Lactuca sativa
spellingShingle Caracterização de fontes de resistência genética contra espécies de Berkeleyomyces e Septoria lactucae em Lactuca sativa
PERDOMO, David Nataren
Alface
Lactuca sativa
Resistência genética
Fungos fitopatogênicos
Septoria lactucae
Berkeleyomyces
Melhoramento genético vegetal
FITOSSANIDADE::FITOPATOLOGIA
title_short Caracterização de fontes de resistência genética contra espécies de Berkeleyomyces e Septoria lactucae em Lactuca sativa
title_full Caracterização de fontes de resistência genética contra espécies de Berkeleyomyces e Septoria lactucae em Lactuca sativa
title_fullStr Caracterização de fontes de resistência genética contra espécies de Berkeleyomyces e Septoria lactucae em Lactuca sativa
title_full_unstemmed Caracterização de fontes de resistência genética contra espécies de Berkeleyomyces e Septoria lactucae em Lactuca sativa
title_sort Caracterização de fontes de resistência genética contra espécies de Berkeleyomyces e Septoria lactucae em Lactuca sativa
author PERDOMO, David Nataren
author_facet PERDOMO, David Nataren
author_role author
dc.contributor.advisor1.fl_str_mv BOITEUX, Leonardo Silva
dc.contributor.advisor-co1.fl_str_mv REIS, Ailton
dc.contributor.advisor-co2.fl_str_mv CABRAL, Cléia Santos
dc.contributor.referee1.fl_str_mv CÂMARA, Marcos Paz Saraiva
dc.contributor.referee2.fl_str_mv GOMES, André Angelo Medeiros
dc.contributor.referee3.fl_str_mv NASCIMENTO, Warley Marcos
dc.contributor.referee4.fl_str_mv GONZÁLEZ ARCOS, Matias
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9708022704070422
dc.contributor.author.fl_str_mv PERDOMO, David Nataren
contributor_str_mv BOITEUX, Leonardo Silva
REIS, Ailton
CABRAL, Cléia Santos
CÂMARA, Marcos Paz Saraiva
GOMES, André Angelo Medeiros
NASCIMENTO, Warley Marcos
GONZÁLEZ ARCOS, Matias
dc.subject.por.fl_str_mv Alface
Lactuca sativa
Resistência genética
Fungos fitopatogênicos
Septoria lactucae
Berkeleyomyces
Melhoramento genético vegetal
topic Alface
Lactuca sativa
Resistência genética
Fungos fitopatogênicos
Septoria lactucae
Berkeleyomyces
Melhoramento genético vegetal
FITOSSANIDADE::FITOPATOLOGIA
dc.subject.cnpq.fl_str_mv FITOSSANIDADE::FITOPATOLOGIA
description Lettuce (Lactuca sativa L.) is the most important leafy vegetable in Brazil and across the world. Lettuce cultivars are grouped into different morphotypes according to their morphological characteristics with special emphasis on the segments ‘Butterhead’, ‘Crispy loose-leaf’, ‘Iceberg’, ‘Cos/Romaine’, ‘Batavian’, and ‘Mimosa’. The yield of lettuce cultivars from the morphotypes ‘Iceberg’ and ‘Butterhead’ have been severely affected by two fungal diseases: Septoria leaf spot (caused by isolates of Septoria lactucae) and black root rot disease (caused by isolates of Berkeleyomyces basicola and B. rouxiae). The present investigation aimed to identify and characterize sources of resistance to S. lactucae as well as against the two Berkeleyomyces species in L. sativa germplasm. Genetic inheritance studies of resistance against isolates of the two Berkeleyomyces species were also performed. Three sets of experiments were carried out at Embrapa Hortaliças in Brasilia-DF during 2021 and 2022. In Chapter 2, forty-two (42) lettuce accessions were evaluated under field conditions (during the rainy season) for reaction to S. lactucae. Nine cultivars categorized as resistant under field conditions were subsequently inoculated with four isolates of S. lactucae under greenhouse conditions. The Area Under the Disease Progress Curve (AACPD) values were significantly lower for the cultivars ‘BRS Mediterrânea’ and ‘Vanda’ (from the ‘Crispy loose-leaf’ morphotype). The other cultivars displayed a tolerance reaction, with intermediate AACPD values, except for the cultivar ‘Rubi’ (control), which displayed moderate susceptibility to the pathogen. In Chapter 3, 68 lettuce cultivars were initially inoculated with an isolate of B. basicola. A second experiment was conducted using 33 cultivars classified as resistant in the first experiment. These accessions were subsequently inoculated with four isolates (two of B. basicola and two of B. rouxiae). A strong correlation was observed between resistance/susceptibility to B. basicola and B. rouxiae. The morphotypes ‘Cos/Romaine’ and ‘Batavian’ showed a higher frequency of resistant accessions. Variation in the reaction to these pathogens was observed among accessions of the morphotypes ‘Crispy loose-leaf’, ‘Mimosa’ and ‘Iceberg’. Most of the lettuces of the ‘Butterhead’ morphotype displayed a susceptible reaction. In Chapter 4, genetic inheritance studies for resistance to both Berkeleyomyces species were carried out under controlled conditions. Different crosses were carried out between the resistant parent ‘La Brillante’ (from the ‘Batavian’ morphotype) and the cultivar ‘Elisa’ (susceptible parent from the ‘Butterhead’ morphotype). Morphological analysis and molecular assays with RAPD markers were used to confirm the hybrid origin of lettuce plants obtained via controlled crosses. Two individual plants were confirmed as F1 hybrids via genotyping and then self-fertilized to obtain two segregating F2 populations. A total of 413 F2 plants were inoculated separately with B. rouxiae (247 plants) and B. basicola (166 plants). Chi-square (χ2) tests at 5% were performed to estimate the best fit of the observed segregation patterns to the predicted Mendelian ratios. Highly contrasting responses were observed between the parents ‘La Brillante’ (resistant) and ‘Elisa’ (susceptible) after inoculation with the two fungi. Segregations in the F2 population displayed a good fit to a dominant monogenic model for resistance against both fungal species. In conclusion, the results obtained in the present work indicate that there are different resistance/susceptibility levels across the lettuce morphotypes in relation to Berkeleyomyces species and S. lactucae. The identification and genetic characterization of novel sources of resistance against these pathogens represents an important contribution to lettuce breeding. The management of these diseases using resistant cultivars can assure higher levels of sustainability in the cultivation of this vegetable in tropical and subtropical regions of Brazil and across the world.
publishDate 2022
dc.date.issued.fl_str_mv 2022-11-18
dc.date.accessioned.fl_str_mv 2023-10-18T20:51:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv PERDOMO, David Nataren. Caracterização de fontes de resistência genética contra espécies de Berkeleyomyces e Septoria lactucae em Lactuca sativa. 2022. 102 f. Tese (Programa de Pós-Graduação em Fitopatologia) - Universidade Federal Rural de Pernambuco, Recife.
dc.identifier.uri.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9412
identifier_str_mv PERDOMO, David Nataren. Caracterização de fontes de resistência genética contra espécies de Berkeleyomyces e Septoria lactucae em Lactuca sativa. 2022. 102 f. Tese (Programa de Pós-Graduação em Fitopatologia) - Universidade Federal Rural de Pernambuco, Recife.
url http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9412
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 1343367238723626701
dc.relation.confidence.fl_str_mv 600
600
600
600
dc.relation.department.fl_str_mv -6800553879972229205
dc.relation.cnpq.fl_str_mv -6207026424523013504
dc.relation.sponsorship.fl_str_mv 2075167498588264571
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Fitopatologia
dc.publisher.initials.fl_str_mv UFRPE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Departamento de Agronomia
publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFRPE
instname:Universidade Federal Rural de Pernambuco (UFRPE)
instacron:UFRPE
instname_str Universidade Federal Rural de Pernambuco (UFRPE)
instacron_str UFRPE
institution UFRPE
reponame_str Biblioteca Digital de Teses e Dissertações da UFRPE
collection Biblioteca Digital de Teses e Dissertações da UFRPE
bitstream.url.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/9412/2/David+Nataren+Perdomo.pdf
http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/9412/1/license.txt
bitstream.checksum.fl_str_mv de7a87a79f10738e1db0c24d4e4f3316
bd3efa91386c1718a7f26a329fdcb468
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)
repository.mail.fl_str_mv bdtd@ufrpe.br ||bdtd@ufrpe.br
_version_ 1810102275782213632