Função dos tricomas vesiculares na tolerância à salinidade em Atriplex nummularia

Detalhes bibliográficos
Autor(a) principal: PAULINO, Martha Katharinne Silva Souza
Data de Publicação: 2020
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9102
Resumo: Epidermal bladders play a fundamental role in the exclusion of toxic ions in some halophyte species. To better understand the role of these structures in the tolerance to salinity, an experiment was carried out with plants of A. nummularia irrigated with waters of three levels of osmotic potential (-0.1; -1.4; -2.7MPa), formulated from NaCl (0; 300 and 600mmol L-1), having plants with Epidermal bladders and plants partially absent from these bladders cells. A randomized block design in a 3 x 2 factorial scheme with five replications was adopted, totaling 30 experimental units. Physiological evaluations were performed (hydric Potential - Ѱw, Osmotic Potential - Ѱo and Pressure - Ѱp, Relative Water Content, Osmotic Adjustment, Pressure-Volume Curve - PV, Liquid photosynthesis, Stomatal conductance, Sweating and Water use efficiency), Extravasation electrolyte, lipid peroxidation and enzyme activity (superoxide dismutase, ascorbate peroxidase, catalase). The experiment was carried out in a protected environment over a period of 30 days. The evaluated results allowed to identify the strong contribution of epidermal bladders in physiological and biochemical relationships. The Ѱo values obtained on the osmometer confirmed that the leakage of inorganic solutes from the bladders cells through maceration are responsible for the most negative osmotic potentials. The parameters obtained with the PV curve allowed to confirm that brushing performed to remove the bladders cells managed to exclude solutes concentrated in the apoplastic space and corrected the discrepancy that occurred between the values accessed by PV curve and by osmometry. Influence of vesicular trichomes on photosynthetic and antioxidant metabolism in Atriplex nummularia plants subjected to salt stress. Chlorophyll fluorescence data demonstrated the integrity of the photosystem component (FSII) even with the removal of bladders cells and elevated salinity. The evaluation of electrolyte leakage, together with the increase in the content of MDA suggest that the partial removal of the vesicles presents a higher probability of oxidative stress. Among the enzyme activities evaluated, APX activity is one of the main mechanisms adopted by Atriplex nummularia in the detoxification of H2O2.
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spelling SOUZA, Edivan Rodrigues deSILVA, Cláudia Ulisses de CarvalhoLINS, Cintia Maria TeixeiraSILVA, Ênio Farias de França eCASTRO, Natália Maria Corte Real dehttp://lattes.cnpq.br/9032644849818857PAULINO, Martha Katharinne Silva Souza2023-06-20T21:25:16Z2020-02-28PAULINO, Martha Katharinne Silva Souza. Função dos tricomas vesiculares na tolerância à salinidade em Atriplex nummularia. 2020. 87 f. Dissertação (Programa de Pós-Graduação em Engenharia Agrícola) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9102Epidermal bladders play a fundamental role in the exclusion of toxic ions in some halophyte species. To better understand the role of these structures in the tolerance to salinity, an experiment was carried out with plants of A. nummularia irrigated with waters of three levels of osmotic potential (-0.1; -1.4; -2.7MPa), formulated from NaCl (0; 300 and 600mmol L-1), having plants with Epidermal bladders and plants partially absent from these bladders cells. A randomized block design in a 3 x 2 factorial scheme with five replications was adopted, totaling 30 experimental units. Physiological evaluations were performed (hydric Potential - Ѱw, Osmotic Potential - Ѱo and Pressure - Ѱp, Relative Water Content, Osmotic Adjustment, Pressure-Volume Curve - PV, Liquid photosynthesis, Stomatal conductance, Sweating and Water use efficiency), Extravasation electrolyte, lipid peroxidation and enzyme activity (superoxide dismutase, ascorbate peroxidase, catalase). The experiment was carried out in a protected environment over a period of 30 days. The evaluated results allowed to identify the strong contribution of epidermal bladders in physiological and biochemical relationships. The Ѱo values obtained on the osmometer confirmed that the leakage of inorganic solutes from the bladders cells through maceration are responsible for the most negative osmotic potentials. The parameters obtained with the PV curve allowed to confirm that brushing performed to remove the bladders cells managed to exclude solutes concentrated in the apoplastic space and corrected the discrepancy that occurred between the values accessed by PV curve and by osmometry. Influence of vesicular trichomes on photosynthetic and antioxidant metabolism in Atriplex nummularia plants subjected to salt stress. Chlorophyll fluorescence data demonstrated the integrity of the photosystem component (FSII) even with the removal of bladders cells and elevated salinity. The evaluation of electrolyte leakage, together with the increase in the content of MDA suggest that the partial removal of the vesicles presents a higher probability of oxidative stress. Among the enzyme activities evaluated, APX activity is one of the main mechanisms adopted by Atriplex nummularia in the detoxification of H2O2.Os tricomas vesiculares desempenham um papel fundamental na exclusão de íons tóxicos em algumas espécies halófitas. Para entender melhor o papel destas estruturas na tolerância a salinidade foi realizado um experimento com plantas de Atriplex nummularia irrigadas com águas de três níveis de potencial osmótico (-0,1;-1,4;-2,7MPa), formulados a partir de NaCl (0; 300 e 600mmol L-1) tendo plantas com tricomas vesiculares e plantas com ausência parcial de tricomas. Adotou-se o delineamento em blocos casualizados em esquema fatorial 3 x 2 com cinco repetições, totalizando 30 unidades experimentais. Foram realizadas avaliações fisiológicas (Potencial Hídrico - Ѱw, Osmótico - Ѱo e de Pressão - Ѱp, Teor Relativo de Água, Ajustamento Osmótico, Curva Pressão-Volume - PV, Fluorescência da clorofila, Fotossíntese líquida, Condutância estomática, Transpiração e Eficiência do uso da água), Extravasamento de eletrólitos, Peroxidação lipídica e Atividade enzimática (Superóxido Dismutase, Ascorbato Peroxidase, Catalase). O experimento foi conduzido em ambiente protegido num período de 30 dias. Os resultados avaliados permitiram identificar a forte contribuição dos tricomas vesiculares nos parâmetros fisiológicos e bioquímicos. Os valores de Ѱo obtidos no osmômetro confirmaram que o vazamento dos solutos inorgânico dos tricomas através da maceração são os responsáveis pelos potenciais osmóticos mais negativos. Os parâmetros obtidos com a curva PV permitiram confirmar que escovação realizada para remoção dos tricomas conseguiu excluir solutos concentrados no espaço apoplástico e corrigiu a discrepância ocorrida entre os valores acessados por curva PV e por osmometria. A presença dos tricomas foram capazes de regular os processos estomáticos de forma que a planta mantenha seu desempenho fotossintético. Dados de fluorescência da clorofila demonstraram a integridade do componente do fotossistema (FSII) mesmo com a remoção dos tricomas e elevação da salinidade. A avaliação de extravasamento de eletrólitos, junto com o aumento do conteúdo de malondialdeído sugerem que a remoção parcial das vesículas apresenta maior probabilidade de estresse oxidativo. A atividade da APX é um dos principais mecanismos adotados pela A. nummularia na desintoxicação de H2O2.Submitted by (ana.araujo@ufrpe.br) on 2023-06-20T21:25:16Z No. of bitstreams: 1 Martha Katharinne Silva Souza Paulino.pdf: 1308391 bytes, checksum: b9f8a346c0c672985d49e45016dadc8e (MD5)Made available in DSpace on 2023-06-20T21:25:16Z (GMT). No. of bitstreams: 1 Martha Katharinne Silva Souza Paulino.pdf: 1308391 bytes, checksum: b9f8a346c0c672985d49e45016dadc8e (MD5) Previous issue date: 2020-02-28Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESConselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Engenharia AgrícolaUFRPEBrasilDepartamento de Engenharia AgrícolaHalófitasAtriplex nummulariaErva-salSalinidadeCIENCIAS AGRARIAS::ENGENHARIA AGRICOLAFunção dos tricomas vesiculares na tolerância à salinidade em Atriplex nummulariaFunction epidermal bladder cells in tolerance to salinity in Atriplex nummulariainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-5347692450416052129600600600600600-286211619635507967491854457215887615552075167498588264571-2555911436985713659info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALMartha Katharinne Silva Souza Paulino.pdfMartha Katharinne Silva Souza Paulino.pdfapplication/pdf1308391http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/9102/2/Martha+Katharinne+Silva+Souza+Paulino.pdfb9f8a346c0c672985d49e45016dadc8eMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/9102/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/91022023-06-20 18:25:16.885oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:37:50.141282Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false
dc.title.por.fl_str_mv Função dos tricomas vesiculares na tolerância à salinidade em Atriplex nummularia
dc.title.alternative.eng.fl_str_mv Function epidermal bladder cells in tolerance to salinity in Atriplex nummularia
title Função dos tricomas vesiculares na tolerância à salinidade em Atriplex nummularia
spellingShingle Função dos tricomas vesiculares na tolerância à salinidade em Atriplex nummularia
PAULINO, Martha Katharinne Silva Souza
Halófitas
Atriplex nummularia
Erva-sal
Salinidade
CIENCIAS AGRARIAS::ENGENHARIA AGRICOLA
title_short Função dos tricomas vesiculares na tolerância à salinidade em Atriplex nummularia
title_full Função dos tricomas vesiculares na tolerância à salinidade em Atriplex nummularia
title_fullStr Função dos tricomas vesiculares na tolerância à salinidade em Atriplex nummularia
title_full_unstemmed Função dos tricomas vesiculares na tolerância à salinidade em Atriplex nummularia
title_sort Função dos tricomas vesiculares na tolerância à salinidade em Atriplex nummularia
author PAULINO, Martha Katharinne Silva Souza
author_facet PAULINO, Martha Katharinne Silva Souza
author_role author
dc.contributor.advisor1.fl_str_mv SOUZA, Edivan Rodrigues de
dc.contributor.advisor-co1.fl_str_mv SILVA, Cláudia Ulisses de Carvalho
dc.contributor.advisor-co2.fl_str_mv LINS, Cintia Maria Teixeira
dc.contributor.referee1.fl_str_mv SILVA, Ênio Farias de França e
dc.contributor.referee2.fl_str_mv CASTRO, Natália Maria Corte Real de
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9032644849818857
dc.contributor.author.fl_str_mv PAULINO, Martha Katharinne Silva Souza
contributor_str_mv SOUZA, Edivan Rodrigues de
SILVA, Cláudia Ulisses de Carvalho
LINS, Cintia Maria Teixeira
SILVA, Ênio Farias de França e
CASTRO, Natália Maria Corte Real de
dc.subject.por.fl_str_mv Halófitas
Atriplex nummularia
Erva-sal
Salinidade
topic Halófitas
Atriplex nummularia
Erva-sal
Salinidade
CIENCIAS AGRARIAS::ENGENHARIA AGRICOLA
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::ENGENHARIA AGRICOLA
description Epidermal bladders play a fundamental role in the exclusion of toxic ions in some halophyte species. To better understand the role of these structures in the tolerance to salinity, an experiment was carried out with plants of A. nummularia irrigated with waters of three levels of osmotic potential (-0.1; -1.4; -2.7MPa), formulated from NaCl (0; 300 and 600mmol L-1), having plants with Epidermal bladders and plants partially absent from these bladders cells. A randomized block design in a 3 x 2 factorial scheme with five replications was adopted, totaling 30 experimental units. Physiological evaluations were performed (hydric Potential - Ѱw, Osmotic Potential - Ѱo and Pressure - Ѱp, Relative Water Content, Osmotic Adjustment, Pressure-Volume Curve - PV, Liquid photosynthesis, Stomatal conductance, Sweating and Water use efficiency), Extravasation electrolyte, lipid peroxidation and enzyme activity (superoxide dismutase, ascorbate peroxidase, catalase). The experiment was carried out in a protected environment over a period of 30 days. The evaluated results allowed to identify the strong contribution of epidermal bladders in physiological and biochemical relationships. The Ѱo values obtained on the osmometer confirmed that the leakage of inorganic solutes from the bladders cells through maceration are responsible for the most negative osmotic potentials. The parameters obtained with the PV curve allowed to confirm that brushing performed to remove the bladders cells managed to exclude solutes concentrated in the apoplastic space and corrected the discrepancy that occurred between the values accessed by PV curve and by osmometry. Influence of vesicular trichomes on photosynthetic and antioxidant metabolism in Atriplex nummularia plants subjected to salt stress. Chlorophyll fluorescence data demonstrated the integrity of the photosystem component (FSII) even with the removal of bladders cells and elevated salinity. The evaluation of electrolyte leakage, together with the increase in the content of MDA suggest that the partial removal of the vesicles presents a higher probability of oxidative stress. Among the enzyme activities evaluated, APX activity is one of the main mechanisms adopted by Atriplex nummularia in the detoxification of H2O2.
publishDate 2020
dc.date.issued.fl_str_mv 2020-02-28
dc.date.accessioned.fl_str_mv 2023-06-20T21:25:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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status_str publishedVersion
dc.identifier.citation.fl_str_mv PAULINO, Martha Katharinne Silva Souza. Função dos tricomas vesiculares na tolerância à salinidade em Atriplex nummularia. 2020. 87 f. Dissertação (Programa de Pós-Graduação em Engenharia Agrícola) - Universidade Federal Rural de Pernambuco, Recife.
dc.identifier.uri.fl_str_mv http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9102
identifier_str_mv PAULINO, Martha Katharinne Silva Souza. Função dos tricomas vesiculares na tolerância à salinidade em Atriplex nummularia. 2020. 87 f. Dissertação (Programa de Pós-Graduação em Engenharia Agrícola) - Universidade Federal Rural de Pernambuco, Recife.
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