Potencial patogênico de Escherichia coli multirresistentes a antibióticos isoladas de carcaças de frango aprovadas para o consumo humano no Estado de Pernambuco, Brasil

• Autor(a) principal: VAZ, Renata Valença
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRPE
• Texto Completo: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4641
• topic: Potencial patogênico; Escherichia coli; Carcaça de frango; Pathogenic potential; Carcass chicken; CIENCIAS AGRARIAS::MEDICINA VETERINARIA

In the present study, we investigated the pathogenic potential of Escherichia coli isolates from samples of chicken livers from carcasses approved for human consumption. The samples ( n = 110 ) were obtained from an abattoir in the State of Pernambuco , Brazil. The bacterial isolates were presumptively identified form Agar Eosin Methylene Blue. The antibiotic resistance profile of the isolates was assessed by the disk diffusion method, according to criteria established by the Committee for Clinical Laboratory Standards Institute (CLSI). Hereafter, ten isolates resistant to three or more antibiotics and ten susceptible isolates were selected and tested for resistance to serum of chicken and human tests. Our results demonstrated the existence of isolates with resistance phenotypes to streptomycin (84.04 %) , tetracycline (44.68 %) , Amikacin (29.78% ) Ceftazidina (21.27 %) and gentamicin (21.27%) . Likewise, in general , the multiresistant isolates showed resistance to the bactericidal effects of serum and human serum birds. The multiresistant isolates (n = 20 ) were phylogenetically investigated and screened for the presence of ISS gene (Increased serum survival). It was observed that the strains were distributed between the four main phylogenetical groups (B2, D, B1, A) and seven isolates of groups B2, B1 and D had the gene iss . In conclusion, our results indicate the presence of E. coli strains multiresistant to antibiotics and potentially pathogenic in chicken carcasses approved for human consumption.