Influência do diâmetro folicular e do hormônio antimulleriano (AMH) na taxa de concepção e produção leiteria de fêmeas da raça Girolando inseminadas
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8809 |
Resumo: | One of the main biotechnologies to improve reproductive rates generating greater economic return for cattle production is artificial insemination, but these indexes are still low, mainly caused by problems in the detection of estrous, late puberty, and postpartum anestrus that affect directly reproductive performance. The size of the ovulatory follicle at the time of insemination among other factors influences the conception rate and reproductive efficiency in fixed-time Artificial Insemination (FTAI) programs. The anti-Müllerian hormone (AMH) is a component of the β transforming growth factor family (TGF-β), known for its attributions in sexual differentiation in males and females, secreted by granulosa and cumulus cells, for exerting impacts on the follicular sensitivity to FSH hormone and initial recruitment of follicles. They regulate the efficiency of follicular selection and recruitment. The objective of this work was to evaluate whether there is a relationship between AMH concentrations with the conception rate in Girolando cows inseminated FTAI in blocks. A total of 327 Girolando cows (Bos taurus indicus x Bos taurus taurus) was used in the experiment. The females were submitted to the following synchronization protocols: for primiparous and multiparous females, the protocol began in D0 with the application of intravaginal progesterone device (CIDR®, 1.9 g, Zoetis) and intramuscular application (IM) 2 mg estradiol benzoate (Gonadiol®, 2 mL, Zoetis); in the D7, application (IM) of 25 mg dinoprost (Lutalyse® 5 mL, Zoetis); D9: application (IM) of 12.5 mg dinoprost (Lutalyse®, 2.5 mL, Zoetis) and 1 mg estradiol cypionate (E.C.P® 0.5 mL Zoetis). For nulliparous cows, changes in the previous protocol were in the Application D7 (IM) of only 12.5 mg dinoprost (Lutalyse®, 2.5mL) and D9 (IM): Application of 12.5 mg dinoprost (Lutalyse®, 2.5 mL) and 0.6 mg of estradiol cypionate (E.C.P® 0.3 mL). After eleven days from the beginning of the protocol (D11), the animals were randomly divided into two groups. Control Group (n:150) and Block Group (n:179). Ultrasound evaluations were initiated an hour earlier from conventional FTAI. After ultrasound evaluation, animals of the Block Group were divided into 4 blocks according to the size of the dominant follicle. Blood collections were performed on the D11 of the protocol, by the coccygeal vein, with heparinized vacuntainer tube. After collection, blood was centrifuged (900 x G for 15 minutes) for plasma removal, which was stored in freezer at -80º C until the analyses were performed. Serum AMH concentrations (ng/mL) were analyzed using bovine anti-Mullerian hormone (Webster, TX). The data were submitted to ANOVA, when significant effect was observed, tukey's test was performed at 5% significance for this, the statistical program RBio was used. The pregnancy rate of the Block Group (70.99%) was 28.71% higher than the Control Group (42.28%). The AMH assay has a measurable analytical interval of 20 ng/mL-0.312 ng/mL. Intraassay coefficients were <= 8% and interassay was <= 12%. As conclusion, AMH concentration cannot be used as a marker for fertility determination in Girolando cows. Further studies will be necessary to use AMH as a molecular marker for milk production. |
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CARNEIRO, Gustavo FerrerGUERRA, Maria Madalena PessoaNETTO MAIA, VictorDUARTE, Sandra SilvaARRUDA, Lúcia Cristina Pereirahttp://lattes.cnpq.br/7625892658716820FRAGA JUNIOR, Antonio Matos2023-02-02T19:24:06Z2020-03-11FRAGA JUNIOR, Antonio Matos. Influência do diâmetro folicular e do hormônio antimulleriano (AMH) na taxa de concepção e produção leiteria de fêmeas da raça Girolando inseminadas. 2020. 75 f. Tese (Programa de Pós-Graduação em Ciência Animal Tropical) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8809One of the main biotechnologies to improve reproductive rates generating greater economic return for cattle production is artificial insemination, but these indexes are still low, mainly caused by problems in the detection of estrous, late puberty, and postpartum anestrus that affect directly reproductive performance. The size of the ovulatory follicle at the time of insemination among other factors influences the conception rate and reproductive efficiency in fixed-time Artificial Insemination (FTAI) programs. The anti-Müllerian hormone (AMH) is a component of the β transforming growth factor family (TGF-β), known for its attributions in sexual differentiation in males and females, secreted by granulosa and cumulus cells, for exerting impacts on the follicular sensitivity to FSH hormone and initial recruitment of follicles. They regulate the efficiency of follicular selection and recruitment. The objective of this work was to evaluate whether there is a relationship between AMH concentrations with the conception rate in Girolando cows inseminated FTAI in blocks. A total of 327 Girolando cows (Bos taurus indicus x Bos taurus taurus) was used in the experiment. The females were submitted to the following synchronization protocols: for primiparous and multiparous females, the protocol began in D0 with the application of intravaginal progesterone device (CIDR®, 1.9 g, Zoetis) and intramuscular application (IM) 2 mg estradiol benzoate (Gonadiol®, 2 mL, Zoetis); in the D7, application (IM) of 25 mg dinoprost (Lutalyse® 5 mL, Zoetis); D9: application (IM) of 12.5 mg dinoprost (Lutalyse®, 2.5 mL, Zoetis) and 1 mg estradiol cypionate (E.C.P® 0.5 mL Zoetis). For nulliparous cows, changes in the previous protocol were in the Application D7 (IM) of only 12.5 mg dinoprost (Lutalyse®, 2.5mL) and D9 (IM): Application of 12.5 mg dinoprost (Lutalyse®, 2.5 mL) and 0.6 mg of estradiol cypionate (E.C.P® 0.3 mL). After eleven days from the beginning of the protocol (D11), the animals were randomly divided into two groups. Control Group (n:150) and Block Group (n:179). Ultrasound evaluations were initiated an hour earlier from conventional FTAI. After ultrasound evaluation, animals of the Block Group were divided into 4 blocks according to the size of the dominant follicle. Blood collections were performed on the D11 of the protocol, by the coccygeal vein, with heparinized vacuntainer tube. After collection, blood was centrifuged (900 x G for 15 minutes) for plasma removal, which was stored in freezer at -80º C until the analyses were performed. Serum AMH concentrations (ng/mL) were analyzed using bovine anti-Mullerian hormone (Webster, TX). The data were submitted to ANOVA, when significant effect was observed, tukey's test was performed at 5% significance for this, the statistical program RBio was used. The pregnancy rate of the Block Group (70.99%) was 28.71% higher than the Control Group (42.28%). The AMH assay has a measurable analytical interval of 20 ng/mL-0.312 ng/mL. Intraassay coefficients were <= 8% and interassay was <= 12%. As conclusion, AMH concentration cannot be used as a marker for fertility determination in Girolando cows. Further studies will be necessary to use AMH as a molecular marker for milk production.Uma das principais biotecnologias para aperfeiçoar os índices reprodutivos e assim gerar maior retorno econômico para a produção de bovinos é a inseminação artificial, porém estes índices ainda se encontram baixos, ocasionados, principalmente, por problemas na detecção do estro, puberdade tardia, e anestro pós-parto que afetam o desempenho reprodutivo. O tamanho do folículo ovulatório no momento da inseminação dentre outros fatores acabam influenciando a taxa de concepção e a eficiência reprodutiva em programas de IATF. O hormônio anti-Mülleriano (AMH) é componente da família do fator de crescimento transformador β (TGF-β), conhecido por suas atribuições na diferenciação sexual em machos e fêmeas, secretado por células da granulosa e cumulus, por exercer impactos sobre a sensibilidade folicular ao hormônio FSH e recrutamento inicial de folículos. Regulam a eficiência do recrutamento e seleção folicular. Objetivou-se com este trabalho avaliar se existe relação entre as concentrações do hormônio antimulleriano com a taxa de concepção em vacas Girolando inseminadas em tempo fixo em blocos. Foram utilizadas 327 vacas da raça Girolando (Bos taurus indicus x Bos taurus taurus) no experimento. As fêmeas foram submetidas aos seguintes protocolos de sincronização: para as fêmeas primíparas, secundíparas e pluríparas o protocolo iniciou-se no D0 com a aplicação do dispositivo intravaginal de progesterona (CIDR®, 1,9 g, Zoetis) e aplicação intramuscular (IM) de 2 mg de benzoato de estradiol (Gonadiol®, 2 mL, Zoetis); no D7 aplicação (IM) de 25 mg de dinoprost (Lutalyse® 5 mL, Zoetis); D9: aplicação (IM) de 12,5 mg de dinoprost (Lutalyse®, 2,5 mL, Zoetis) e 1 mg de cipionato de estradiol (E.C.P® 0,5 mL Zoetis). Para as nulíparas as modificações no protocolo anterior foram no D7 aplicação (IM) de apenas 12,5 mg de dinoprost (Lutalyse®, 2,5mL) e D9- aplicação de (IM) de 12,5 mg de dinoprost (Lutalyse®, 2,5 mL) e 0,6 mg de cipionato de estradiol (E.C.P® 0,3 mL). Decorridos onze dias do início do protocolo (D11) os animais foram divididos randomicamente em dois grupos. Grupo Controle (n:150) e Grupo Bloco (n:179). Avaliações ultrassonográficas foram iniciadas, uma hora antes, da IATF convencional. Após a avaliação ultrassonográfica os animais do Grupo Bloco foram divididos em 4 blocos de acordo com o tamanho do folículo dominante. As coletas de sangue foram realizadas no dia 11 do protocolo, pela veia coccígea, com tubo vacuntainer heparinizado. Após a coleta, o sangue foi centrifugado (900Xg durante 15 minutos) para a retirada do plasma, que foi armazenado em freezer a -80º C até a realização das análises. As concentrações séricas de AMH (ng/mL) foram analisadas utilizando o Bovine Anti-Mullerian hormone (Webster, TX). Os dados foram submetidos a análise de variância (ANOVA), e quando constatado efeito significativo foi realizado teste de Tukey a 5% de significância para tal utilizou-se o programa estatístico RBio. A taxa de concepção do grupo bloco (70,99%) foi 28,71% maior que o grupo controle (42,28%). O ensaio para dosagem do AMH tem um intervalo analítico mensurável de 20 ng/mL-0.312 ng/mL. Os coeficientes de variação intraensaio foi de <= 8% e interensaio foram <= 12%. Os níveis de AMH não influenciaram no diâmetro do folículo dominante (p>0,05), assim como na taxa de concepção, entretanto exerceram influência positiva (p<0,05) sobre a produção de leite. Concluímos que a dosagem do AMH não pode ser utilizada como um marcador para determinação da fertilidade em vacas da raça Girolando. Maiores estudos serão necessários visando a utilização do AMH como marcador molecular para produção de leite.Submitted by (lucia.rodrigues@ufrpe.br) on 2023-02-02T19:24:06Z No. of bitstreams: 1 Antonio Matos Fraga Junior.pdf: 630876 bytes, checksum: d3b2d1c4f4b9cace9af897d0713b3d3f (MD5)Made available in DSpace on 2023-02-02T19:24:06Z (GMT). No. of bitstreams: 1 Antonio Matos Fraga Junior.pdf: 630876 bytes, checksum: d3b2d1c4f4b9cace9af897d0713b3d3f (MD5) Previous issue date: 2020-03-11Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Ciência Animal TropicalUFRPEBrasilDepartamento de Morfologia e Fisiologia AnimalGirolandoFertilidadeHormônio antimullerianoProdução de leiteInseminação artificialCIENCIAS AGRARIAS::MEDICINA VETERINARIAInfluência do diâmetro folicular e do hormônio antimulleriano (AMH) na taxa de concepção e produção leiteria de fêmeas da raça Girolando inseminadasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-6143187600765506511600600600600-89223641879873962044536702642350173192075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALAntonio Matos Fraga Junior.pdfAntonio Matos Fraga Junior.pdfapplication/pdf630876http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/8809/2/Antonio+Matos+Fraga+Junior.pdfd3b2d1c4f4b9cace9af897d0713b3d3fMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/8809/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/88092023-02-02 16:24:06.947oai:tede2:tede2/8809Tk9UQTogQ09MT1FVRSBBUVVJIEEgU1VBIFBSw5NQUklBIExJQ0VOw4dBCkVzdGEgbGljZW7Dp2EgZGUgZXhlbXBsbyDDqSBmb3JuZWNpZGEgYXBlbmFzIHBhcmEgZmlucyBpbmZvcm1hdGl2b3MuCgpMSUNFTsOHQSBERSBESVNUUklCVUnDh8ODTyBOw4NPLUVYQ0xVU0lWQQoKQ29tIGEgYXByZXNlbnRhw6fDo28gZGVzdGEgbGljZW7Dp2EsIHZvY8OqIChvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgClhYWCAoU2lnbGEgZGEgVW5pdmVyc2lkYWRlKSBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBwb2RlLCBzZW0gYWx0ZXJhciBvIGNvbnRlw7pkbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIApwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgU2lnbGEgZGUgVW5pdmVyc2lkYWRlIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPDs3BpYSBhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyAKbmVzdGEgbGljZW7Dp2EuIFZvY8OqIHRhbWLDqW0gZGVjbGFyYSBxdWUgbyBkZXDDs3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBuw6NvLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZcO6ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEHDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSAKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBTSUdMQSBERSAKVU5JVkVSU0lEQURFLCBWT0PDiiBERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVPDg08gQ09NTyAKVEFNQsOJTSBBUyBERU1BSVMgT0JSSUdBw4fDlUVTIEVYSUdJREFTIFBPUiBDT05UUkFUTyBPVSBBQ09SRE8uCgpBIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgo=Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2023-02-02T19:24:06Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Influência do diâmetro folicular e do hormônio antimulleriano (AMH) na taxa de concepção e produção leiteria de fêmeas da raça Girolando inseminadas |
title |
Influência do diâmetro folicular e do hormônio antimulleriano (AMH) na taxa de concepção e produção leiteria de fêmeas da raça Girolando inseminadas |
spellingShingle |
Influência do diâmetro folicular e do hormônio antimulleriano (AMH) na taxa de concepção e produção leiteria de fêmeas da raça Girolando inseminadas FRAGA JUNIOR, Antonio Matos Girolando Fertilidade Hormônio antimulleriano Produção de leite Inseminação artificial CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Influência do diâmetro folicular e do hormônio antimulleriano (AMH) na taxa de concepção e produção leiteria de fêmeas da raça Girolando inseminadas |
title_full |
Influência do diâmetro folicular e do hormônio antimulleriano (AMH) na taxa de concepção e produção leiteria de fêmeas da raça Girolando inseminadas |
title_fullStr |
Influência do diâmetro folicular e do hormônio antimulleriano (AMH) na taxa de concepção e produção leiteria de fêmeas da raça Girolando inseminadas |
title_full_unstemmed |
Influência do diâmetro folicular e do hormônio antimulleriano (AMH) na taxa de concepção e produção leiteria de fêmeas da raça Girolando inseminadas |
title_sort |
Influência do diâmetro folicular e do hormônio antimulleriano (AMH) na taxa de concepção e produção leiteria de fêmeas da raça Girolando inseminadas |
author |
FRAGA JUNIOR, Antonio Matos |
author_facet |
FRAGA JUNIOR, Antonio Matos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
CARNEIRO, Gustavo Ferrer |
dc.contributor.referee1.fl_str_mv |
GUERRA, Maria Madalena Pessoa |
dc.contributor.referee2.fl_str_mv |
NETTO MAIA, Victor |
dc.contributor.referee3.fl_str_mv |
DUARTE, Sandra Silva |
dc.contributor.referee4.fl_str_mv |
ARRUDA, Lúcia Cristina Pereira |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7625892658716820 |
dc.contributor.author.fl_str_mv |
FRAGA JUNIOR, Antonio Matos |
contributor_str_mv |
CARNEIRO, Gustavo Ferrer GUERRA, Maria Madalena Pessoa NETTO MAIA, Victor DUARTE, Sandra Silva ARRUDA, Lúcia Cristina Pereira |
dc.subject.por.fl_str_mv |
Girolando Fertilidade Hormônio antimulleriano Produção de leite Inseminação artificial |
topic |
Girolando Fertilidade Hormônio antimulleriano Produção de leite Inseminação artificial CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
One of the main biotechnologies to improve reproductive rates generating greater economic return for cattle production is artificial insemination, but these indexes are still low, mainly caused by problems in the detection of estrous, late puberty, and postpartum anestrus that affect directly reproductive performance. The size of the ovulatory follicle at the time of insemination among other factors influences the conception rate and reproductive efficiency in fixed-time Artificial Insemination (FTAI) programs. The anti-Müllerian hormone (AMH) is a component of the β transforming growth factor family (TGF-β), known for its attributions in sexual differentiation in males and females, secreted by granulosa and cumulus cells, for exerting impacts on the follicular sensitivity to FSH hormone and initial recruitment of follicles. They regulate the efficiency of follicular selection and recruitment. The objective of this work was to evaluate whether there is a relationship between AMH concentrations with the conception rate in Girolando cows inseminated FTAI in blocks. A total of 327 Girolando cows (Bos taurus indicus x Bos taurus taurus) was used in the experiment. The females were submitted to the following synchronization protocols: for primiparous and multiparous females, the protocol began in D0 with the application of intravaginal progesterone device (CIDR®, 1.9 g, Zoetis) and intramuscular application (IM) 2 mg estradiol benzoate (Gonadiol®, 2 mL, Zoetis); in the D7, application (IM) of 25 mg dinoprost (Lutalyse® 5 mL, Zoetis); D9: application (IM) of 12.5 mg dinoprost (Lutalyse®, 2.5 mL, Zoetis) and 1 mg estradiol cypionate (E.C.P® 0.5 mL Zoetis). For nulliparous cows, changes in the previous protocol were in the Application D7 (IM) of only 12.5 mg dinoprost (Lutalyse®, 2.5mL) and D9 (IM): Application of 12.5 mg dinoprost (Lutalyse®, 2.5 mL) and 0.6 mg of estradiol cypionate (E.C.P® 0.3 mL). After eleven days from the beginning of the protocol (D11), the animals were randomly divided into two groups. Control Group (n:150) and Block Group (n:179). Ultrasound evaluations were initiated an hour earlier from conventional FTAI. After ultrasound evaluation, animals of the Block Group were divided into 4 blocks according to the size of the dominant follicle. Blood collections were performed on the D11 of the protocol, by the coccygeal vein, with heparinized vacuntainer tube. After collection, blood was centrifuged (900 x G for 15 minutes) for plasma removal, which was stored in freezer at -80º C until the analyses were performed. Serum AMH concentrations (ng/mL) were analyzed using bovine anti-Mullerian hormone (Webster, TX). The data were submitted to ANOVA, when significant effect was observed, tukey's test was performed at 5% significance for this, the statistical program RBio was used. The pregnancy rate of the Block Group (70.99%) was 28.71% higher than the Control Group (42.28%). The AMH assay has a measurable analytical interval of 20 ng/mL-0.312 ng/mL. Intraassay coefficients were <= 8% and interassay was <= 12%. As conclusion, AMH concentration cannot be used as a marker for fertility determination in Girolando cows. Further studies will be necessary to use AMH as a molecular marker for milk production. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-03-11 |
dc.date.accessioned.fl_str_mv |
2023-02-02T19:24:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FRAGA JUNIOR, Antonio Matos. Influência do diâmetro folicular e do hormônio antimulleriano (AMH) na taxa de concepção e produção leiteria de fêmeas da raça Girolando inseminadas. 2020. 75 f. Tese (Programa de Pós-Graduação em Ciência Animal Tropical) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8809 |
identifier_str_mv |
FRAGA JUNIOR, Antonio Matos. Influência do diâmetro folicular e do hormônio antimulleriano (AMH) na taxa de concepção e produção leiteria de fêmeas da raça Girolando inseminadas. 2020. 75 f. Tese (Programa de Pós-Graduação em Ciência Animal Tropical) - Universidade Federal Rural de Pernambuco, Recife. |
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http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8809 |
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openAccess |
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Universidade Federal Rural de Pernambuco |
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Programa de Pós-Graduação em Ciência Animal Tropical |
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UFRPE |
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Departamento de Morfologia e Fisiologia Animal |
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Universidade Federal Rural de Pernambuco |
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Biblioteca Digital de Teses e Dissertações da UFRPE |
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Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE) |
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bdtd@ufrpe.br ||bdtd@ufrpe.br |
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