Caracterização estrutural, funcional e diversidade de osmoprotetores no transcriptoma de Stylosanthes scabra (Vogel) sob déficit hídrico
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Texto Completo: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9507 |
Resumo: | Abiotic stress conditions like water deficit affect plant growth causing morphological, physiological, biochemical and molecular changes, changing genes expression that activate biochemical and physiological responses, such as compatible solutes accumulation. Osmoprotectants are highly soluble compounds that act primarily on cellular osmotic adjustment maintaining their turgidity (cell volume) even with low water potential. Molecular and computational approaches have made possible new perspectives on the structure and function of proteins and genes already known and have proven efficient in studies with known genome species, however, few studies have been conducted with native species. Stylosanthes scabra, a legume with high forage potencial, it is Brazilian semiarid native specie and has drought tolerance. The present work aimed to identify and characterize transcripts belonging to different classes of osmoprotectants in S. scabra transcriptome of under water deficit as well as validate its expression in RT-qPCR. Initially, candidate sequences for osmoprotectants genes were obtained from S. scabra database proceeding with data annotation, translation and identification of conserved domains through in silico methods. A total of 74 osmoprotectants sequences with conserved domains were identified for S. scabra: 40 of Trehalose (TPS and TPP), 17 of Myo inositol (MIPS and IMP), 10 of Glycine betaine (BADH and CMO) and 7 of Proline (P5CS and P5CR) equivalent results described in other plants. These were anchored in the A. duranensis genome (n = 10) revealing a total of 19 anchored loci similar to the results that has been found in soybean, another Fabaceae specie. Phenetic orthologous trees of osmoprotectants genes demonstrated the clustering of S. scabra sequences with other Fabaceae species, proving their similarity and pointing out to these protein conservation. The in silico differential gene expression analysis was performed using RNA seq data, where the 35 transcripts were differentially expressed, presenting induction and repression in both 6 h and 24 h stress periods showing that these genes participate in S. scabra water deficit response. These results prove to be valuable for genetic breeding of S. scabra and other legumes of agricultural importance. |
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CARVALHO, Reginaldo deSILVA, Roberta Lane de OliveiraBEZERRA NETO, João PacíficoMELO, Natoniel Franklin deFERREIRA NETO, José Ribamar Costahttp://lattes.cnpq.br/0466650010755074BARROS, Agnes Angélica Guedes de2024-02-19T14:16:18Z2019-07-31BARROS, Agnes Angélica Guedes de. Caracterização estrutural, funcional e diversidade de osmoprotetores no transcriptoma de Stylosanthes scabra (Vogel) sob déficit hídrico. 2019. 135 f. Dissertação (Programa de Pós-Graduação em Melhoramento Genético de Plantas) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9507Abiotic stress conditions like water deficit affect plant growth causing morphological, physiological, biochemical and molecular changes, changing genes expression that activate biochemical and physiological responses, such as compatible solutes accumulation. Osmoprotectants are highly soluble compounds that act primarily on cellular osmotic adjustment maintaining their turgidity (cell volume) even with low water potential. Molecular and computational approaches have made possible new perspectives on the structure and function of proteins and genes already known and have proven efficient in studies with known genome species, however, few studies have been conducted with native species. Stylosanthes scabra, a legume with high forage potencial, it is Brazilian semiarid native specie and has drought tolerance. The present work aimed to identify and characterize transcripts belonging to different classes of osmoprotectants in S. scabra transcriptome of under water deficit as well as validate its expression in RT-qPCR. Initially, candidate sequences for osmoprotectants genes were obtained from S. scabra database proceeding with data annotation, translation and identification of conserved domains through in silico methods. A total of 74 osmoprotectants sequences with conserved domains were identified for S. scabra: 40 of Trehalose (TPS and TPP), 17 of Myo inositol (MIPS and IMP), 10 of Glycine betaine (BADH and CMO) and 7 of Proline (P5CS and P5CR) equivalent results described in other plants. These were anchored in the A. duranensis genome (n = 10) revealing a total of 19 anchored loci similar to the results that has been found in soybean, another Fabaceae specie. Phenetic orthologous trees of osmoprotectants genes demonstrated the clustering of S. scabra sequences with other Fabaceae species, proving their similarity and pointing out to these protein conservation. The in silico differential gene expression analysis was performed using RNA seq data, where the 35 transcripts were differentially expressed, presenting induction and repression in both 6 h and 24 h stress periods showing that these genes participate in S. scabra water deficit response. These results prove to be valuable for genetic breeding of S. scabra and other legumes of agricultural importance.Estresses abióticos, como o déficit hídrico, prejudicam o desenvolvimento das plantas causando mudanças morfológicas, fisiológicas, bioquímicas e moleculares, alterando a expressão de genes que ativam respostas bioquímicas e fisiológicas, como por exemplo, o acúmulo de solutos compatíveis. Os osmoprotetores são moléculas altamente solúveis que atuam principalmente no ajuste osmótico celular mantendo a sua turgidez (volume celular) mesmo com o potencial hídrico baixo. Abordagens moleculares e computacionais têm possibilitado novas perspectivas sobre a estrutura e função de proteínas e genes já conhecidos e se mostrado eficientes em estudos com espécies de genoma conhecido, no entanto, poucos estudos têm sido conduzidos com espécies nativas. Stylosanthes scabra, leguminosa com alto potencial forrageiro, é nativa do semi-árido brasileiro e apresenta tolerância à seca. O presente projeto teve como objetivo identificar e caracterizar transcritos candidatos pertencentes a diferentes classes de osmoprotetores no transcriptoma de S. scabra sob déficit hídrico. Inicialmente, sequências candidatas para genes osmoprotetores foram obtidas no banco de dados de S. scabra, procedendo-se com a anotação dos dados, tradução e identificação dos domínios conservados por meio de ferramentas in silico. Um total de 74 sequências de osmoprotetores com domínios conservados foram identificadas em S. scabra, sendo 40 de Trealose (TPS e TPP), 17 de Mio inositol (MIPS e IMP), 10 de Glicina betaína (BADH e CMO) e 7 de Prolina (P5CS e P5CR) resultados equivalentes ao descrito em outras espécies vegetais. Estes foram ancorados no genoma de Arachis duranensis (n=10) revelando um total de 19 loci ancorados similar ao encontrado em soja, outra espécie do gênero Fabaceae. Árvores fenéticas de ortólogos de genes de osmoprotetores demonstraram o agrupamento das sequências de S. scabra com outras espécies da família Fabaceae, comprovando sua similaridade e apontando uma conservação destas proteínas. A análise de expressão gênica diferencial in silico foi realizada utilizando dados de RNA-Seq onde dos 74 transcritos 35 mostraram-se diferencialmente expressos, apresentando indução e repressão em ambos os tempos de 6 h e 24 h de estresse indicando que estes participam da resposta do vegetal ao déficit hídrico. Esses resultados mostram-se valiosos para o melhoramento genético da espécie S. scabra e de outras leguminosas de importância agrícola.Submitted by (ana.araujo@ufrpe.br) on 2024-02-19T14:16:18Z No. of bitstreams: 1 Agnes Angelica Guedes de Barros.pdf: 4177240 bytes, checksum: af5f86858eca316c47b619422be5f431 (MD5)Made available in DSpace on 2024-02-19T14:16:18Z (GMT). No. of bitstreams: 1 Agnes Angelica Guedes de Barros.pdf: 4177240 bytes, checksum: af5f86858eca316c47b619422be5f431 (MD5) Previous issue date: 2019-07-31Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Melhoramento Genético de PlantasUFRPEBrasilDepartamento de AgronomiaLeguminosaStylosanthesDéficit hídricoExpressão gênicaFITOTECNIA::MELHORAMENTO VEGETALCaracterização estrutural, funcional e diversidade de osmoprotetores no transcriptoma de Stylosanthes scabra (Vogel) sob déficit hídricoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-6234655866848882505600600600600-680055387997222920526156072994701319672075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPEORIGINALAgnes Angelica Guedes de Barros.pdfAgnes Angelica Guedes de Barros.pdfapplication/pdf4177240http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/9507/2/Agnes+Angelica+Guedes+de+Barros.pdfaf5f86858eca316c47b619422be5f431MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/9507/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede2/95072024-02-19 11:16:19.07oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2024-05-28T12:38:21.964790Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Caracterização estrutural, funcional e diversidade de osmoprotetores no transcriptoma de Stylosanthes scabra (Vogel) sob déficit hídrico |
title |
Caracterização estrutural, funcional e diversidade de osmoprotetores no transcriptoma de Stylosanthes scabra (Vogel) sob déficit hídrico |
spellingShingle |
Caracterização estrutural, funcional e diversidade de osmoprotetores no transcriptoma de Stylosanthes scabra (Vogel) sob déficit hídrico BARROS, Agnes Angélica Guedes de Leguminosa Stylosanthes Déficit hídrico Expressão gênica FITOTECNIA::MELHORAMENTO VEGETAL |
title_short |
Caracterização estrutural, funcional e diversidade de osmoprotetores no transcriptoma de Stylosanthes scabra (Vogel) sob déficit hídrico |
title_full |
Caracterização estrutural, funcional e diversidade de osmoprotetores no transcriptoma de Stylosanthes scabra (Vogel) sob déficit hídrico |
title_fullStr |
Caracterização estrutural, funcional e diversidade de osmoprotetores no transcriptoma de Stylosanthes scabra (Vogel) sob déficit hídrico |
title_full_unstemmed |
Caracterização estrutural, funcional e diversidade de osmoprotetores no transcriptoma de Stylosanthes scabra (Vogel) sob déficit hídrico |
title_sort |
Caracterização estrutural, funcional e diversidade de osmoprotetores no transcriptoma de Stylosanthes scabra (Vogel) sob déficit hídrico |
author |
BARROS, Agnes Angélica Guedes de |
author_facet |
BARROS, Agnes Angélica Guedes de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
CARVALHO, Reginaldo de |
dc.contributor.advisor-co1.fl_str_mv |
SILVA, Roberta Lane de Oliveira |
dc.contributor.advisor-co2.fl_str_mv |
BEZERRA NETO, João Pacífico |
dc.contributor.referee1.fl_str_mv |
MELO, Natoniel Franklin de |
dc.contributor.referee2.fl_str_mv |
FERREIRA NETO, José Ribamar Costa |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0466650010755074 |
dc.contributor.author.fl_str_mv |
BARROS, Agnes Angélica Guedes de |
contributor_str_mv |
CARVALHO, Reginaldo de SILVA, Roberta Lane de Oliveira BEZERRA NETO, João Pacífico MELO, Natoniel Franklin de FERREIRA NETO, José Ribamar Costa |
dc.subject.por.fl_str_mv |
Leguminosa Stylosanthes Déficit hídrico Expressão gênica |
topic |
Leguminosa Stylosanthes Déficit hídrico Expressão gênica FITOTECNIA::MELHORAMENTO VEGETAL |
dc.subject.cnpq.fl_str_mv |
FITOTECNIA::MELHORAMENTO VEGETAL |
description |
Abiotic stress conditions like water deficit affect plant growth causing morphological, physiological, biochemical and molecular changes, changing genes expression that activate biochemical and physiological responses, such as compatible solutes accumulation. Osmoprotectants are highly soluble compounds that act primarily on cellular osmotic adjustment maintaining their turgidity (cell volume) even with low water potential. Molecular and computational approaches have made possible new perspectives on the structure and function of proteins and genes already known and have proven efficient in studies with known genome species, however, few studies have been conducted with native species. Stylosanthes scabra, a legume with high forage potencial, it is Brazilian semiarid native specie and has drought tolerance. The present work aimed to identify and characterize transcripts belonging to different classes of osmoprotectants in S. scabra transcriptome of under water deficit as well as validate its expression in RT-qPCR. Initially, candidate sequences for osmoprotectants genes were obtained from S. scabra database proceeding with data annotation, translation and identification of conserved domains through in silico methods. A total of 74 osmoprotectants sequences with conserved domains were identified for S. scabra: 40 of Trehalose (TPS and TPP), 17 of Myo inositol (MIPS and IMP), 10 of Glycine betaine (BADH and CMO) and 7 of Proline (P5CS and P5CR) equivalent results described in other plants. These were anchored in the A. duranensis genome (n = 10) revealing a total of 19 anchored loci similar to the results that has been found in soybean, another Fabaceae specie. Phenetic orthologous trees of osmoprotectants genes demonstrated the clustering of S. scabra sequences with other Fabaceae species, proving their similarity and pointing out to these protein conservation. The in silico differential gene expression analysis was performed using RNA seq data, where the 35 transcripts were differentially expressed, presenting induction and repression in both 6 h and 24 h stress periods showing that these genes participate in S. scabra water deficit response. These results prove to be valuable for genetic breeding of S. scabra and other legumes of agricultural importance. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019-07-31 |
dc.date.accessioned.fl_str_mv |
2024-02-19T14:16:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BARROS, Agnes Angélica Guedes de. Caracterização estrutural, funcional e diversidade de osmoprotetores no transcriptoma de Stylosanthes scabra (Vogel) sob déficit hídrico. 2019. 135 f. Dissertação (Programa de Pós-Graduação em Melhoramento Genético de Plantas) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9507 |
identifier_str_mv |
BARROS, Agnes Angélica Guedes de. Caracterização estrutural, funcional e diversidade de osmoprotetores no transcriptoma de Stylosanthes scabra (Vogel) sob déficit hídrico. 2019. 135 f. Dissertação (Programa de Pós-Graduação em Melhoramento Genético de Plantas) - Universidade Federal Rural de Pernambuco, Recife. |
url |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/9507 |
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openAccess |
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