Expressão de MicroRNAs e dos genes da interleucina 2 e fator de necrose tumoral e suas correlações com o H. pylori

• Autor(a) principal: Zabaglia, Luanna Munhoz
• Data de Publicação: 2017
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da USC
• Texto Completo: http://tede2.usc.br:8080/jspui/handle/tede/339
• topic: Doenças gástricas; Helicobacter pylori; Interleucina 2; MicroRNAs; TNF-α; Gastric disease; Helicobacter pylori; Interleukin 2; MicroRNAs; TNF-α; CIENCIAS EXATAS E DA TERRA

The miRNAs appear to play an important role in controlling the expression of several genes, including IL-2 and TNF-α. Thus, 294 samples of gastric biopsies from dyspeptic patients were submitted to expression analysis by PCR in Real Time and polymorphisms by PCR-RFLP, rs2069762 (IL-2: + 114T> G), rs2069762 (IL- -330T> G) and rs1800629 (TNF-α: -308G> A), of the IL-2 and TNF-α genes. In parallel, the expression levels of miRNAs 146a, 155, and 181c were also evaluated. We found a statistically significant association between the presence of H. pylori and the development of gastritis and gastric cancer (CA). As for IL-2 polymorphisms, the genotype T/T of the +114 T>G and the G/G of the -330 T>G showed to be a risk factor for CA. The haplotype + 114T / -330G was also associated with CA. Allied to the above, the A/A genotype of the -308 G>A polymorphism of the TNF-α gene was also associated with the risk of CA development. As for gene expression, for IL-2, there was no statistically significant difference. On the other hand, we observed a statistically significant increase in the expression of TNF-α in the gastritis group in relation to the control, and a statistically significant decrease in the CA group also in relation to the control. Similar results were found considering the presence of H. pylori. There was also a statistically significant increase in the expression of miRNA 146a and 155 in the gastritis group in relation to the control, in contrast there was a statistically significant decrease in the expression of miRNA 181c in the cancer group. Finally, our data revealed the involvement of miRNA 146a and 155 in the control of IL-2 and TNF-α genes as well as the involvement of miRNA 181c in the etiology of gastric cancer